Barbel circovirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus

Average proteome isoelectric point is 8.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F5BSB5|F5BSB5_9CIRC Capsid protein OS=Barbel circovirus OX=759938 GN=cap PE=3 SV=1
MM1 pKa = 7.75PKK3 pKa = 9.01ATSKK7 pKa = 9.18TRR9 pKa = 11.84HH10 pKa = 5.44LQNPRR15 pKa = 11.84QDD17 pKa = 3.35GPRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 4.15QPVKK26 pKa = 10.25RR27 pKa = 11.84WCFTLNNPTAEE38 pKa = 4.32EE39 pKa = 4.01RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 4.71IQQIITADD50 pKa = 3.52SVDD53 pKa = 4.0FAVIGNEE60 pKa = 3.95IGDD63 pKa = 3.75SGTPHH68 pKa = 6.49LQGFLNMKK76 pKa = 7.28TKK78 pKa = 10.51RR79 pKa = 11.84RR80 pKa = 11.84LGTMKK85 pKa = 10.1KK86 pKa = 8.63WFNARR91 pKa = 11.84AQYY94 pKa = 9.66EE95 pKa = 4.27AAKK98 pKa = 9.29GTDD101 pKa = 3.62LQNDD105 pKa = 4.57EE106 pKa = 4.7YY107 pKa = 9.78CTKK110 pKa = 10.96GGDD113 pKa = 3.06TYY115 pKa = 11.6LRR117 pKa = 11.84IGEE120 pKa = 4.24PGKK123 pKa = 10.12EE124 pKa = 3.56RR125 pKa = 11.84CRR127 pKa = 11.84NDD129 pKa = 3.11LQKK132 pKa = 11.12AIDD135 pKa = 3.72VVKK138 pKa = 10.5RR139 pKa = 11.84SSGSMRR145 pKa = 11.84AVAEE149 pKa = 4.09ACPATFIRR157 pKa = 11.84YY158 pKa = 8.59GRR160 pKa = 11.84GLRR163 pKa = 11.84DD164 pKa = 3.42YY165 pKa = 10.83ANVMQYY171 pKa = 10.48RR172 pKa = 11.84KK173 pKa = 9.61PRR175 pKa = 11.84DD176 pKa = 3.24FKK178 pKa = 11.06TEE180 pKa = 3.72VKK182 pKa = 10.61VYY184 pKa = 10.72VGDD187 pKa = 4.13PGCSKK192 pKa = 10.46SRR194 pKa = 11.84KK195 pKa = 9.41ASEE198 pKa = 4.2LCAGTTVYY206 pKa = 10.58YY207 pKa = 10.42KK208 pKa = 10.5PRR210 pKa = 11.84GMWWDD215 pKa = 4.0GYY217 pKa = 11.01DD218 pKa = 3.41GQEE221 pKa = 3.94NVIVDD226 pKa = 4.48DD227 pKa = 4.59FYY229 pKa = 11.78GWMPCDD235 pKa = 3.13EE236 pKa = 4.86LLRR239 pKa = 11.84VFDD242 pKa = 5.32RR243 pKa = 11.84YY244 pKa = 9.32PCKK247 pKa = 10.88VPVKK251 pKa = 8.87GAYY254 pKa = 9.61VEE256 pKa = 4.72FVSTAIYY263 pKa = 8.36VTSNKK268 pKa = 9.79HH269 pKa = 3.04VWQWYY274 pKa = 8.76KK275 pKa = 11.04FEE277 pKa = 4.81GFDD280 pKa = 3.3PAAVMRR286 pKa = 11.84RR287 pKa = 11.84VNVYY291 pKa = 10.17LVYY294 pKa = 10.96DD295 pKa = 3.64NAGEE299 pKa = 4.02RR300 pKa = 11.84FVNLRR305 pKa = 11.84EE306 pKa = 3.93SAMYY310 pKa = 10.6DD311 pKa = 3.67PVTMRR316 pKa = 11.84YY317 pKa = 9.75CYY319 pKa = 10.8

Molecular weight: 36.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F5BSB5|F5BSB5_9CIRC Capsid protein OS=Barbel circovirus OX=759938 GN=cap PE=3 SV=1
MM1 pKa = 7.59LLPRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84ARR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84AGRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84TRR19 pKa = 11.84GWRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 8.38YY26 pKa = 10.49VRR28 pKa = 11.84RR29 pKa = 11.84QRR31 pKa = 11.84HH32 pKa = 5.21RR33 pKa = 11.84GRR35 pKa = 11.84YY36 pKa = 8.33SYY38 pKa = 11.06FRR40 pKa = 11.84LRR42 pKa = 11.84AIKK45 pKa = 9.94HH46 pKa = 6.29DD47 pKa = 3.7HH48 pKa = 5.8LTWNASNGMSSGIVFLMADD67 pKa = 3.43MSYY70 pKa = 10.67PKK72 pKa = 10.51NYY74 pKa = 8.12WDD76 pKa = 3.73YY77 pKa = 9.92YY78 pKa = 9.79KK79 pKa = 10.27ISKK82 pKa = 10.0VVVQLWPEE90 pKa = 4.15FNNVPINADD99 pKa = 3.65RR100 pKa = 11.84DD101 pKa = 3.74KK102 pKa = 11.47VLYY105 pKa = 10.65GSTAVDD111 pKa = 3.4YY112 pKa = 11.31DD113 pKa = 4.18DD114 pKa = 4.76ATMATSTADD123 pKa = 3.3PFKK126 pKa = 10.68DD127 pKa = 3.48YY128 pKa = 11.09SSRR131 pKa = 11.84KK132 pKa = 8.2MFISNRR138 pKa = 11.84THH140 pKa = 6.61IRR142 pKa = 11.84IFTPRR147 pKa = 11.84PNIEE151 pKa = 4.08IYY153 pKa = 10.44KK154 pKa = 10.47SSASTNYY161 pKa = 9.48GFLNIRR167 pKa = 11.84SGAWINTVHH176 pKa = 7.32DD177 pKa = 4.47GVPHH181 pKa = 6.13YY182 pKa = 10.42GLKK185 pKa = 9.62IWLPSNTGAPSAIGYY200 pKa = 9.61KK201 pKa = 10.42LIIKK205 pKa = 9.47YY206 pKa = 10.12YY207 pKa = 8.4VLFRR211 pKa = 11.84NRR213 pKa = 11.84II214 pKa = 3.49

Molecular weight: 25.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

533

214

319

266.5

31.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.754 ± 0.135

1.689 ± 1.075

6.004 ± 0.55

3.565 ± 1.675

3.752 ± 0.009

6.754 ± 0.73

1.876 ± 0.591

4.878 ± 1.655

6.379 ± 0.491

4.878 ± 0.465

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.002 ± 0.126

5.066 ± 0.643

4.878 ± 0.131

2.627 ± 1.078

10.882 ± 1.7

5.441 ± 1.594

5.441 ± 0.192

6.942 ± 1.148

2.439 ± 0.232

6.754 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski