Vibrio phage JSF33

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Enhodamvirus; unclassified Enhodamvirus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0YKK1|A0A2D0YKK1_9CAUD Uncharacterized protein OS=Vibrio phage JSF33 OX=1983610 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 10.53SIAEE6 pKa = 3.95YY7 pKa = 10.01AARR10 pKa = 11.84GVEE13 pKa = 4.12INDD16 pKa = 3.73LALVEE21 pKa = 3.97WDD23 pKa = 3.24MDD25 pKa = 3.73GGIAAWNGDD34 pKa = 3.33HH35 pKa = 6.7NLQVVAEE42 pKa = 4.25RR43 pKa = 11.84NDD45 pKa = 3.67DD46 pKa = 3.29GSYY49 pKa = 10.23TMEE52 pKa = 4.16GLVDD56 pKa = 3.62GEE58 pKa = 4.39RR59 pKa = 11.84YY60 pKa = 9.69SVLFTVDD67 pKa = 3.06HH68 pKa = 7.75DD69 pKa = 4.26EE70 pKa = 4.45LQEE73 pKa = 3.98EE74 pKa = 4.35ALDD77 pKa = 3.96VAFTAYY83 pKa = 10.64VAMLGEE89 pKa = 4.3

Molecular weight: 9.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D0YME1|A0A2D0YME1_9CAUD Uncharacterized protein OS=Vibrio phage JSF33 OX=1983610 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.11KK3 pKa = 10.2YY4 pKa = 9.94EE5 pKa = 4.21PNYY8 pKa = 9.44KK9 pKa = 10.07VSIIAGDD16 pKa = 3.15KK17 pKa = 9.69GRR19 pKa = 11.84FYY21 pKa = 11.36VVRR24 pKa = 11.84LKK26 pKa = 10.92DD27 pKa = 3.55SNGGYY32 pKa = 10.33EE33 pKa = 4.24GGTFIISRR41 pKa = 11.84MVGTRR46 pKa = 11.84NYY48 pKa = 8.89TRR50 pKa = 11.84FYY52 pKa = 11.13KK53 pKa = 9.14VTPDD57 pKa = 3.53SKK59 pKa = 11.53NKK61 pKa = 9.09TSYY64 pKa = 10.83PLFPTLRR71 pKa = 11.84DD72 pKa = 3.31AIMAVVAA79 pKa = 4.67

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

11748

53

782

255.4

28.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.457 ± 0.431

0.86 ± 0.118

6.376 ± 0.325

6.639 ± 0.539

3.303 ± 0.193

8.231 ± 0.766

1.719 ± 0.174

5.363 ± 0.21

5.848 ± 0.485

7.022 ± 0.237

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.243 ± 0.286

4.733 ± 0.296

3.83 ± 0.178

3.958 ± 0.228

5.337 ± 0.269

6.759 ± 0.408

5.626 ± 0.38

6.708 ± 0.347

1.762 ± 0.168

3.226 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski