Amino acid dipepetide frequency for Vibrio phage JSF33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.896AlaAla: 10.896 ± 1.424
0.596AlaCys: 0.596 ± 0.242
5.193AlaAsp: 5.193 ± 0.701
7.491AlaGlu: 7.491 ± 1.172
2.979AlaPhe: 2.979 ± 0.358
6.299AlaGly: 6.299 ± 0.693
1.617AlaHis: 1.617 ± 0.393
5.108AlaIle: 5.108 ± 0.662
6.64AlaLys: 6.64 ± 1.265
6.725AlaLeu: 6.725 ± 0.817
3.15AlaMet: 3.15 ± 0.476
2.639AlaAsn: 2.639 ± 0.408
3.065AlaPro: 3.065 ± 0.522
4.342AlaGln: 4.342 ± 0.613
4.512AlaArg: 4.512 ± 0.489
6.47AlaSer: 6.47 ± 0.819
5.789AlaThr: 5.789 ± 0.783
6.895AlaVal: 6.895 ± 0.721
1.447AlaTrp: 1.447 ± 0.382
2.724AlaTyr: 2.724 ± 0.546
0.0AlaXaa: 0.0 ± 0.0
Cys
0.511CysAla: 0.511 ± 0.18
0.17CysCys: 0.17 ± 0.116
0.255CysAsp: 0.255 ± 0.152
0.426CysGlu: 0.426 ± 0.19
0.341CysPhe: 0.341 ± 0.131
0.936CysGly: 0.936 ± 0.269
0.341CysHis: 0.341 ± 0.144
0.596CysIle: 0.596 ± 0.228
0.681CysLys: 0.681 ± 0.254
0.596CysLeu: 0.596 ± 0.261
0.17CysMet: 0.17 ± 0.114
0.17CysAsn: 0.17 ± 0.116
0.426CysPro: 0.426 ± 0.23
0.511CysGln: 0.511 ± 0.196
0.426CysArg: 0.426 ± 0.193
0.511CysSer: 0.511 ± 0.221
0.936CysThr: 0.936 ± 0.33
0.426CysVal: 0.426 ± 0.189
0.085CysTrp: 0.085 ± 0.074
0.085CysTyr: 0.085 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
5.363AspAla: 5.363 ± 0.692
0.851AspCys: 0.851 ± 0.274
3.831AspAsp: 3.831 ± 0.698
4.767AspGlu: 4.767 ± 0.897
2.809AspPhe: 2.809 ± 0.497
5.704AspGly: 5.704 ± 0.941
0.511AspHis: 0.511 ± 0.219
3.575AspIle: 3.575 ± 0.54
4.256AspLys: 4.256 ± 0.616
5.448AspLeu: 5.448 ± 0.756
2.043AspMet: 2.043 ± 0.435
1.958AspAsn: 1.958 ± 0.419
3.575AspPro: 3.575 ± 0.522
1.022AspGln: 1.022 ± 0.315
3.065AspArg: 3.065 ± 0.661
4.342AspSer: 4.342 ± 0.68
3.575AspThr: 3.575 ± 0.573
4.256AspVal: 4.256 ± 0.551
1.022AspTrp: 1.022 ± 0.354
1.788AspTyr: 1.788 ± 0.435
0.0AspXaa: 0.0 ± 0.0
Glu
5.533GluAla: 5.533 ± 0.678
0.511GluCys: 0.511 ± 0.208
4.682GluAsp: 4.682 ± 0.51
5.363GluGlu: 5.363 ± 0.912
1.873GluPhe: 1.873 ± 0.364
3.831GluGly: 3.831 ± 0.735
1.617GluHis: 1.617 ± 0.433
3.575GluIle: 3.575 ± 0.654
3.235GluLys: 3.235 ± 0.588
6.214GluLeu: 6.214 ± 0.789
2.809GluMet: 2.809 ± 0.492
2.639GluAsn: 2.639 ± 0.484
1.788GluPro: 1.788 ± 0.479
3.661GluGln: 3.661 ± 0.522
4.086GluArg: 4.086 ± 0.719
3.405GluSer: 3.405 ± 0.573
3.065GluThr: 3.065 ± 0.519
4.852GluVal: 4.852 ± 0.651
1.703GluTrp: 1.703 ± 0.382
1.958GluTyr: 1.958 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
3.065PheAla: 3.065 ± 0.503
0.255PheCys: 0.255 ± 0.127
2.469PheAsp: 2.469 ± 0.378
1.532PheGlu: 1.532 ± 0.423
0.851PhePhe: 0.851 ± 0.4
3.661PheGly: 3.661 ± 0.686
0.341PheHis: 0.341 ± 0.186
1.277PheIle: 1.277 ± 0.25
1.788PheLys: 1.788 ± 0.455
2.384PheLeu: 2.384 ± 0.371
0.936PheMet: 0.936 ± 0.344
2.213PheAsn: 2.213 ± 0.427
1.107PhePro: 1.107 ± 0.272
1.447PheGln: 1.447 ± 0.441
1.788PheArg: 1.788 ± 0.419
2.469PheSer: 2.469 ± 0.519
2.213PheThr: 2.213 ± 0.423
1.958PheVal: 1.958 ± 0.512
0.255PheTrp: 0.255 ± 0.146
1.022PheTyr: 1.022 ± 0.332
0.0PheXaa: 0.0 ± 0.0
Gly
6.129GlyAla: 6.129 ± 0.93
0.681GlyCys: 0.681 ± 0.326
5.108GlyAsp: 5.108 ± 0.567
3.661GlyGlu: 3.661 ± 0.468
2.554GlyPhe: 2.554 ± 0.455
10.896GlyGly: 10.896 ± 4.177
1.532GlyHis: 1.532 ± 0.373
3.746GlyIle: 3.746 ± 0.641
4.427GlyLys: 4.427 ± 0.727
5.023GlyLeu: 5.023 ± 0.519
2.213GlyMet: 2.213 ± 0.6
5.533GlyAsn: 5.533 ± 1.198
1.617GlyPro: 1.617 ± 0.375
3.405GlyGln: 3.405 ± 0.427
4.086GlyArg: 4.086 ± 0.541
6.214GlySer: 6.214 ± 1.052
5.448GlyThr: 5.448 ± 0.985
5.108GlyVal: 5.108 ± 0.661
1.107GlyTrp: 1.107 ± 0.281
3.831GlyTyr: 3.831 ± 0.493
0.0GlyXaa: 0.0 ± 0.0
His
1.022HisAla: 1.022 ± 0.366
0.17HisCys: 0.17 ± 0.127
1.022HisAsp: 1.022 ± 0.284
1.022HisGlu: 1.022 ± 0.278
0.511HisPhe: 0.511 ± 0.246
1.703HisGly: 1.703 ± 0.488
0.17HisHis: 0.17 ± 0.117
0.766HisIle: 0.766 ± 0.275
0.681HisLys: 0.681 ± 0.253
1.447HisLeu: 1.447 ± 0.394
0.681HisMet: 0.681 ± 0.241
0.766HisAsn: 0.766 ± 0.22
0.766HisPro: 0.766 ± 0.3
0.766HisGln: 0.766 ± 0.262
1.703HisArg: 1.703 ± 0.433
0.936HisSer: 0.936 ± 0.293
0.851HisThr: 0.851 ± 0.209
1.277HisVal: 1.277 ± 0.34
0.17HisTrp: 0.17 ± 0.119
0.766HisTyr: 0.766 ± 0.313
0.0HisXaa: 0.0 ± 0.0
Ile
6.214IleAla: 6.214 ± 0.686
0.341IleCys: 0.341 ± 0.159
4.001IleAsp: 4.001 ± 0.661
3.916IleGlu: 3.916 ± 0.716
0.851IlePhe: 0.851 ± 0.403
3.916IleGly: 3.916 ± 0.599
0.511IleHis: 0.511 ± 0.216
2.724IleIle: 2.724 ± 0.452
3.405IleLys: 3.405 ± 0.533
2.384IleLeu: 2.384 ± 0.49
1.532IleMet: 1.532 ± 0.347
2.809IleAsn: 2.809 ± 0.462
3.065IlePro: 3.065 ± 0.399
3.065IleGln: 3.065 ± 0.523
3.661IleArg: 3.661 ± 0.705
3.916IleSer: 3.916 ± 0.56
2.554IleThr: 2.554 ± 0.654
2.894IleVal: 2.894 ± 0.511
1.107IleTrp: 1.107 ± 0.367
0.766IleTyr: 0.766 ± 0.249
0.0IleXaa: 0.0 ± 0.0
Lys
5.789LysAla: 5.789 ± 0.999
0.255LysCys: 0.255 ± 0.123
3.49LysAsp: 3.49 ± 0.679
3.661LysGlu: 3.661 ± 0.606
1.703LysPhe: 1.703 ± 0.382
5.448LysGly: 5.448 ± 0.809
1.192LysHis: 1.192 ± 0.335
2.894LysIle: 2.894 ± 0.449
4.682LysLys: 4.682 ± 0.784
3.575LysLeu: 3.575 ± 0.564
2.554LysMet: 2.554 ± 0.559
2.724LysAsn: 2.724 ± 0.574
2.298LysPro: 2.298 ± 0.465
1.873LysGln: 1.873 ± 0.43
2.809LysArg: 2.809 ± 0.595
4.001LysSer: 4.001 ± 0.587
2.128LysThr: 2.128 ± 0.504
3.746LysVal: 3.746 ± 0.542
0.936LysTrp: 0.936 ± 0.325
2.724LysTyr: 2.724 ± 0.502
0.0LysXaa: 0.0 ± 0.0
Leu
6.895LeuAla: 6.895 ± 0.761
0.681LeuCys: 0.681 ± 0.202
5.023LeuAsp: 5.023 ± 0.656
4.001LeuGlu: 4.001 ± 0.666
1.873LeuPhe: 1.873 ± 0.362
6.385LeuGly: 6.385 ± 0.751
1.532LeuHis: 1.532 ± 0.34
4.512LeuIle: 4.512 ± 0.728
3.831LeuLys: 3.831 ± 0.592
3.746LeuLeu: 3.746 ± 0.476
2.554LeuMet: 2.554 ± 0.51
3.49LeuAsn: 3.49 ± 0.554
3.15LeuPro: 3.15 ± 0.53
2.384LeuGln: 2.384 ± 0.367
4.427LeuArg: 4.427 ± 0.528
4.171LeuSer: 4.171 ± 0.466
4.171LeuThr: 4.171 ± 0.5
5.278LeuVal: 5.278 ± 0.699
1.022LeuTrp: 1.022 ± 0.376
1.107LeuTyr: 1.107 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
3.661MetAla: 3.661 ± 0.843
0.596MetCys: 0.596 ± 0.219
1.788MetAsp: 1.788 ± 0.312
2.554MetGlu: 2.554 ± 0.448
0.851MetPhe: 0.851 ± 0.263
2.213MetGly: 2.213 ± 0.469
0.766MetHis: 0.766 ± 0.205
1.192MetIle: 1.192 ± 0.305
1.958MetLys: 1.958 ± 0.327
2.128MetLeu: 2.128 ± 0.384
1.362MetMet: 1.362 ± 0.322
1.362MetAsn: 1.362 ± 0.329
1.277MetPro: 1.277 ± 0.378
1.362MetGln: 1.362 ± 0.432
1.703MetArg: 1.703 ± 0.492
2.724MetSer: 2.724 ± 0.525
1.532MetThr: 1.532 ± 0.323
1.958MetVal: 1.958 ± 0.541
0.341MetTrp: 0.341 ± 0.192
1.107MetTyr: 1.107 ± 0.332
0.0MetXaa: 0.0 ± 0.0
Asn
4.852AsnAla: 4.852 ± 0.692
0.426AsnCys: 0.426 ± 0.165
2.128AsnAsp: 2.128 ± 0.506
3.15AsnGlu: 3.15 ± 0.583
1.362AsnPhe: 1.362 ± 0.278
5.704AsnGly: 5.704 ± 1.347
0.681AsnHis: 0.681 ± 0.217
2.213AsnIle: 2.213 ± 0.396
2.384AsnLys: 2.384 ± 0.387
2.639AsnLeu: 2.639 ± 0.35
1.362AsnMet: 1.362 ± 0.312
2.639AsnAsn: 2.639 ± 0.451
1.617AsnPro: 1.617 ± 0.443
1.958AsnGln: 1.958 ± 0.377
2.894AsnArg: 2.894 ± 0.592
3.32AsnSer: 3.32 ± 0.691
2.894AsnThr: 2.894 ± 0.449
2.043AsnVal: 2.043 ± 0.315
1.532AsnTrp: 1.532 ± 0.451
1.532AsnTyr: 1.532 ± 0.337
0.0AsnXaa: 0.0 ± 0.0
Pro
4.171ProAla: 4.171 ± 0.635
0.341ProCys: 0.341 ± 0.182
3.916ProAsp: 3.916 ± 0.458
3.065ProGlu: 3.065 ± 0.577
1.703ProPhe: 1.703 ± 0.343
0.255ProGly: 0.255 ± 0.161
0.766ProHis: 0.766 ± 0.274
2.128ProIle: 2.128 ± 0.367
1.447ProLys: 1.447 ± 0.361
2.298ProLeu: 2.298 ± 0.517
0.851ProMet: 0.851 ± 0.396
2.213ProAsn: 2.213 ± 0.403
1.362ProPro: 1.362 ± 0.452
1.447ProGln: 1.447 ± 0.297
1.617ProArg: 1.617 ± 0.44
3.916ProSer: 3.916 ± 0.589
3.15ProThr: 3.15 ± 0.577
1.873ProVal: 1.873 ± 0.447
0.596ProTrp: 0.596 ± 0.296
1.107ProTyr: 1.107 ± 0.434
0.0ProXaa: 0.0 ± 0.0
Gln
4.086GlnAla: 4.086 ± 0.642
0.085GlnCys: 0.085 ± 0.084
1.873GlnAsp: 1.873 ± 0.382
2.894GlnGlu: 2.894 ± 0.535
1.022GlnPhe: 1.022 ± 0.26
2.554GlnGly: 2.554 ± 0.37
0.766GlnHis: 0.766 ± 0.239
2.384GlnIle: 2.384 ± 0.409
1.532GlnLys: 1.532 ± 0.264
3.15GlnLeu: 3.15 ± 0.563
1.617GlnMet: 1.617 ± 0.37
1.447GlnAsn: 1.447 ± 0.413
1.703GlnPro: 1.703 ± 0.414
1.703GlnGln: 1.703 ± 0.426
2.213GlnArg: 2.213 ± 0.574
2.469GlnSer: 2.469 ± 0.406
2.979GlnThr: 2.979 ± 0.433
2.298GlnVal: 2.298 ± 0.374
1.192GlnTrp: 1.192 ± 0.264
1.617GlnTyr: 1.617 ± 0.387
0.0GlnXaa: 0.0 ± 0.0
Arg
4.427ArgAla: 4.427 ± 0.651
0.596ArgCys: 0.596 ± 0.228
2.979ArgAsp: 2.979 ± 0.439
3.32ArgGlu: 3.32 ± 0.449
2.809ArgPhe: 2.809 ± 0.698
4.086ArgGly: 4.086 ± 0.744
0.596ArgHis: 0.596 ± 0.325
3.235ArgIle: 3.235 ± 0.492
3.405ArgLys: 3.405 ± 0.589
5.108ArgLeu: 5.108 ± 0.656
2.469ArgMet: 2.469 ± 0.413
2.809ArgAsn: 2.809 ± 0.73
1.788ArgPro: 1.788 ± 0.436
1.617ArgGln: 1.617 ± 0.378
2.809ArgArg: 2.809 ± 0.408
2.979ArgSer: 2.979 ± 0.492
2.213ArgThr: 2.213 ± 0.437
3.065ArgVal: 3.065 ± 0.507
1.277ArgTrp: 1.277 ± 0.45
1.788ArgTyr: 1.788 ± 0.398
0.0ArgXaa: 0.0 ± 0.0
Ser
5.789SerAla: 5.789 ± 0.907
0.341SerCys: 0.341 ± 0.171
5.023SerAsp: 5.023 ± 0.68
3.916SerGlu: 3.916 ± 0.531
2.724SerPhe: 2.724 ± 0.461
5.193SerGly: 5.193 ± 0.834
1.107SerHis: 1.107 ± 0.29
4.001SerIle: 4.001 ± 0.52
3.916SerLys: 3.916 ± 0.658
4.597SerLeu: 4.597 ± 0.582
2.384SerMet: 2.384 ± 0.361
3.746SerAsn: 3.746 ± 0.864
3.32SerPro: 3.32 ± 0.601
2.213SerGln: 2.213 ± 0.338
2.894SerArg: 2.894 ± 0.561
4.597SerSer: 4.597 ± 0.683
3.235SerThr: 3.235 ± 0.828
5.363SerVal: 5.363 ± 0.748
1.022SerTrp: 1.022 ± 0.323
2.213SerTyr: 2.213 ± 0.484
0.0SerXaa: 0.0 ± 0.0
Thr
5.959ThrAla: 5.959 ± 0.837
0.426ThrCys: 0.426 ± 0.209
3.065ThrAsp: 3.065 ± 0.575
3.49ThrGlu: 3.49 ± 0.564
2.298ThrPhe: 2.298 ± 0.432
5.533ThrGly: 5.533 ± 0.716
0.851ThrHis: 0.851 ± 0.296
3.065ThrIle: 3.065 ± 0.524
3.235ThrLys: 3.235 ± 0.462
4.597ThrLeu: 4.597 ± 0.571
1.362ThrMet: 1.362 ± 0.409
2.213ThrAsn: 2.213 ± 0.497
2.724ThrPro: 2.724 ± 0.403
2.469ThrGln: 2.469 ± 0.444
2.384ThrArg: 2.384 ± 0.447
2.979ThrSer: 2.979 ± 0.493
2.639ThrThr: 2.639 ± 0.562
4.512ThrVal: 4.512 ± 0.606
0.681ThrTrp: 0.681 ± 0.441
1.788ThrTyr: 1.788 ± 0.428
0.0ThrXaa: 0.0 ± 0.0
Val
5.618ValAla: 5.618 ± 0.693
0.511ValCys: 0.511 ± 0.196
4.682ValAsp: 4.682 ± 0.647
4.852ValGlu: 4.852 ± 0.565
1.873ValPhe: 1.873 ± 0.522
5.023ValGly: 5.023 ± 0.669
1.277ValHis: 1.277 ± 0.297
3.746ValIle: 3.746 ± 0.611
4.171ValLys: 4.171 ± 0.817
4.852ValLeu: 4.852 ± 0.624
1.703ValMet: 1.703 ± 0.466
2.979ValAsn: 2.979 ± 0.698
2.043ValPro: 2.043 ± 0.362
2.469ValGln: 2.469 ± 0.466
3.405ValArg: 3.405 ± 0.587
4.937ValSer: 4.937 ± 0.926
3.575ValThr: 3.575 ± 0.647
5.448ValVal: 5.448 ± 0.6
1.277ValTrp: 1.277 ± 0.303
2.639ValTyr: 2.639 ± 0.545
0.0ValXaa: 0.0 ± 0.0
Trp
1.192TrpAla: 1.192 ± 0.276
0.341TrpCys: 0.341 ± 0.161
0.936TrpAsp: 0.936 ± 0.275
0.936TrpGlu: 0.936 ± 0.319
0.851TrpPhe: 0.851 ± 0.418
0.681TrpGly: 0.681 ± 0.207
0.426TrpHis: 0.426 ± 0.211
1.107TrpIle: 1.107 ± 0.268
1.022TrpLys: 1.022 ± 0.242
1.532TrpLeu: 1.532 ± 0.425
0.426TrpMet: 0.426 ± 0.193
0.681TrpAsn: 0.681 ± 0.258
0.255TrpPro: 0.255 ± 0.17
0.426TrpGln: 0.426 ± 0.185
1.277TrpArg: 1.277 ± 0.308
1.362TrpSer: 1.362 ± 0.275
1.788TrpThr: 1.788 ± 0.372
1.362TrpVal: 1.362 ± 0.312
0.341TrpTrp: 0.341 ± 0.131
0.681TrpTyr: 0.681 ± 0.209
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.405TyrAla: 3.405 ± 0.505
0.426TyrCys: 0.426 ± 0.218
2.298TyrAsp: 2.298 ± 0.392
2.384TyrGlu: 2.384 ± 0.603
1.192TyrPhe: 1.192 ± 0.263
2.298TyrGly: 2.298 ± 0.412
0.596TyrHis: 0.596 ± 0.201
1.788TyrIle: 1.788 ± 0.391
1.788TyrLys: 1.788 ± 0.512
1.788TyrLeu: 1.788 ± 0.414
0.17TyrMet: 0.17 ± 0.106
2.298TyrAsn: 2.298 ± 0.391
1.362TyrPro: 1.362 ± 0.293
1.447TyrGln: 1.447 ± 0.312
1.617TyrArg: 1.617 ± 0.422
1.873TyrSer: 1.873 ± 0.51
1.532TyrThr: 1.532 ± 0.284
2.469TyrVal: 2.469 ± 0.375
0.511TyrTrp: 0.511 ± 0.239
1.022TyrTyr: 1.022 ± 0.312
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 46 proteins (11748 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski