Synechococcus phage Syn19
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 215 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E3SQ31|E3SQ31_9CAUD Uncharacterized protein OS=Synechococcus phage Syn19 OX=445684 GN=Syn19_066 PE=4 SV=1
MM1 pKa = 7.85 FDD3 pKa = 3.11 TTLDD7 pKa = 4.04 LFCHH11 pKa = 6.47 NDD13 pKa = 3.15 NLNDD17 pKa = 4.04 RR18 pKa = 11.84 DD19 pKa = 3.95 HH20 pKa = 7.14 MIDD23 pKa = 4.92 QMAQLYY29 pKa = 8.69 FRR31 pKa = 11.84 AMTTNANKK39 pKa = 10.7 GEE41 pKa = 4.12 NFDD44 pKa = 5.86 AIACYY49 pKa = 9.72 EE50 pKa = 4.09 EE51 pKa = 4.53 WVVDD55 pKa = 4.35 GVDD58 pKa = 3.81 PQDD61 pKa = 2.99 VDD63 pKa = 3.83 YY64 pKa = 11.22 EE65 pKa = 4.76 FIFAPDD71 pKa = 3.22 LTEE74 pKa = 3.87 EE75 pKa = 4.24 RR76 pKa = 11.84 EE77 pKa = 4.26 EE78 pKa = 4.09 NN79 pKa = 3.44
Molecular weight: 9.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.49
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|E3SQC5|E3SQC5_9CAUD Uncharacterized protein OS=Synechococcus phage Syn19 OX=445684 GN=Syn19_160 PE=4 SV=1
MM1 pKa = 7.95 RR2 pKa = 11.84 DD3 pKa = 2.96 RR4 pKa = 11.84 YY5 pKa = 9.93 KK6 pKa = 11.07 RR7 pKa = 11.84 NIRR10 pKa = 11.84 YY11 pKa = 9.4 YY12 pKa = 8.29 EE13 pKa = 3.97 TGFTTRR19 pKa = 11.84 LFLYY23 pKa = 10.5 NLLNTIGCLRR33 pKa = 11.84 GPHH36 pKa = 5.92 NKK38 pKa = 8.0 TRR40 pKa = 11.84 LL41 pKa = 3.44
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 10.116
IPC_protein 10.95
Toseland 10.584
ProMoST 10.496
Dawson 10.774
Bjellqvist 10.584
Wikipedia 11.067
Rodwell 10.833
Grimsley 10.877
Solomon 10.891
Lehninger 10.847
Nozaki 10.584
DTASelect 10.584
Thurlkill 10.643
EMBOSS 11.008
Sillero 10.701
Patrickios 10.599
IPC_peptide 10.891
IPC2_peptide 9.78
IPC2.peptide.svr19 7.943
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
215
0
215
56525
25
6483
262.9
29.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.151 ± 0.262
0.808 ± 0.094
6.581 ± 0.152
6.029 ± 0.281
4.448 ± 0.133
8.081 ± 0.482
1.559 ± 0.133
6.047 ± 0.215
5.787 ± 0.357
7.271 ± 0.182
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.197 ± 0.207
5.727 ± 0.171
3.929 ± 0.165
3.804 ± 0.099
4.2 ± 0.16
7.006 ± 0.219
7.499 ± 0.429
6.599 ± 0.184
1.141 ± 0.087
4.136 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here