Amino acid dipepetide frequency for Synechococcus phage Syn19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.006AlaAla: 7.006 ± 0.493
0.495AlaCys: 0.495 ± 0.126
4.069AlaAsp: 4.069 ± 0.33
4.158AlaGlu: 4.158 ± 0.36
3.521AlaPhe: 3.521 ± 0.281
6.174AlaGly: 6.174 ± 0.382
1.008AlaHis: 1.008 ± 0.136
3.981AlaIle: 3.981 ± 0.261
3.698AlaLys: 3.698 ± 0.291
4.989AlaLeu: 4.989 ± 0.331
1.433AlaMet: 1.433 ± 0.227
3.963AlaAsn: 3.963 ± 0.353
2.795AlaPro: 2.795 ± 0.235
2.901AlaGln: 2.901 ± 0.205
2.76AlaArg: 2.76 ± 0.288
5.148AlaSer: 5.148 ± 0.424
5.962AlaThr: 5.962 ± 0.729
4.759AlaVal: 4.759 ± 0.327
0.478AlaTrp: 0.478 ± 0.085
2.211AlaTyr: 2.211 ± 0.174
0.0AlaXaa: 0.0 ± 0.0
Cys
0.389CysAla: 0.389 ± 0.087
0.071CysCys: 0.071 ± 0.05
0.69CysAsp: 0.69 ± 0.134
0.566CysGlu: 0.566 ± 0.125
0.354CysPhe: 0.354 ± 0.079
0.513CysGly: 0.513 ± 0.114
0.212CysHis: 0.212 ± 0.064
0.389CysIle: 0.389 ± 0.095
0.548CysLys: 0.548 ± 0.123
0.655CysLeu: 0.655 ± 0.11
0.265CysMet: 0.265 ± 0.087
0.318CysAsn: 0.318 ± 0.105
0.248CysPro: 0.248 ± 0.072
0.354CysGln: 0.354 ± 0.104
0.354CysArg: 0.354 ± 0.074
0.602CysSer: 0.602 ± 0.127
0.425CysThr: 0.425 ± 0.088
0.548CysVal: 0.548 ± 0.106
0.177CysTrp: 0.177 ± 0.059
0.407CysTyr: 0.407 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
5.06AspAla: 5.06 ± 0.316
0.743AspCys: 0.743 ± 0.151
4.246AspAsp: 4.246 ± 0.304
3.928AspGlu: 3.928 ± 0.28
3.131AspPhe: 3.131 ± 0.256
6.139AspGly: 6.139 ± 0.418
1.15AspHis: 1.15 ± 0.181
3.804AspIle: 3.804 ± 0.325
3.379AspLys: 3.379 ± 0.335
4.458AspLeu: 4.458 ± 0.327
1.486AspMet: 1.486 ± 0.231
3.379AspAsn: 3.379 ± 0.28
3.485AspPro: 3.485 ± 0.273
2.282AspGln: 2.282 ± 0.201
2.548AspArg: 2.548 ± 0.279
3.804AspSer: 3.804 ± 0.278
4.564AspThr: 4.564 ± 0.416
4.034AspVal: 4.034 ± 0.374
1.008AspTrp: 1.008 ± 0.13
3.184AspTyr: 3.184 ± 0.266
0.0AspXaa: 0.0 ± 0.0
Glu
3.538GluAla: 3.538 ± 0.337
0.513GluCys: 0.513 ± 0.116
4.175GluAsp: 4.175 ± 0.334
4.724GluGlu: 4.724 ± 0.607
2.954GluPhe: 2.954 ± 0.276
4.051GluGly: 4.051 ± 0.322
1.044GluHis: 1.044 ± 0.19
4.246GluIle: 4.246 ± 0.332
3.662GluLys: 3.662 ± 0.478
4.954GluLeu: 4.954 ± 0.405
1.557GluMet: 1.557 ± 0.243
3.025GluAsn: 3.025 ± 0.258
1.645GluPro: 1.645 ± 0.207
2.495GluGln: 2.495 ± 0.267
2.884GluArg: 2.884 ± 0.282
3.644GluSer: 3.644 ± 0.301
3.574GluThr: 3.574 ± 0.304
4.299GluVal: 4.299 ± 0.304
0.796GluTrp: 0.796 ± 0.126
2.512GluTyr: 2.512 ± 0.204
0.0GluXaa: 0.0 ± 0.0
Phe
2.866PheAla: 2.866 ± 0.249
0.478PheCys: 0.478 ± 0.104
3.698PheAsp: 3.698 ± 0.223
2.601PheGlu: 2.601 ± 0.229
1.999PhePhe: 1.999 ± 0.254
3.273PheGly: 3.273 ± 0.401
0.725PheHis: 0.725 ± 0.133
2.742PheIle: 2.742 ± 0.25
2.495PheLys: 2.495 ± 0.285
3.131PheLeu: 3.131 ± 0.255
1.026PheMet: 1.026 ± 0.179
3.025PheAsn: 3.025 ± 0.236
1.663PhePro: 1.663 ± 0.195
1.716PheGln: 1.716 ± 0.16
1.751PheArg: 1.751 ± 0.158
3.361PheSer: 3.361 ± 0.258
3.273PheThr: 3.273 ± 0.343
2.76PheVal: 2.76 ± 0.258
0.354PheTrp: 0.354 ± 0.084
1.539PheTyr: 1.539 ± 0.134
0.0PheXaa: 0.0 ± 0.0
Gly
5.944GlyAla: 5.944 ± 0.505
0.548GlyCys: 0.548 ± 0.115
4.883GlyAsp: 4.883 ± 0.473
4.158GlyGlu: 4.158 ± 0.241
3.291GlyPhe: 3.291 ± 0.235
9.111GlyGly: 9.111 ± 1.422
1.451GlyHis: 1.451 ± 0.184
4.405GlyIle: 4.405 ± 0.388
4.016GlyLys: 4.016 ± 0.429
5.148GlyLeu: 5.148 ± 0.443
1.769GlyMet: 1.769 ± 0.284
4.547GlyAsn: 4.547 ± 0.437
2.035GlyPro: 2.035 ± 0.266
3.149GlyGln: 3.149 ± 0.276
2.972GlyArg: 2.972 ± 0.258
6.935GlySer: 6.935 ± 0.628
6.847GlyThr: 6.847 ± 0.699
5.077GlyVal: 5.077 ± 0.351
1.026GlyTrp: 1.026 ± 0.123
3.485GlyTyr: 3.485 ± 0.27
0.0GlyXaa: 0.0 ± 0.0
His
0.743HisAla: 0.743 ± 0.142
0.177HisCys: 0.177 ± 0.062
0.991HisAsp: 0.991 ± 0.176
1.026HisGlu: 1.026 ± 0.179
0.743HisPhe: 0.743 ± 0.135
1.15HisGly: 1.15 ± 0.159
0.407HisHis: 0.407 ± 0.104
0.832HisIle: 0.832 ± 0.139
0.778HisLys: 0.778 ± 0.143
1.238HisLeu: 1.238 ± 0.207
0.513HisMet: 0.513 ± 0.118
0.814HisAsn: 0.814 ± 0.157
0.832HisPro: 0.832 ± 0.134
0.637HisGln: 0.637 ± 0.115
0.778HisArg: 0.778 ± 0.129
1.008HisSer: 1.008 ± 0.153
1.026HisThr: 1.026 ± 0.135
0.902HisVal: 0.902 ± 0.126
0.23HisTrp: 0.23 ± 0.073
0.761HisTyr: 0.761 ± 0.13
0.0HisXaa: 0.0 ± 0.0
Ile
4.281IleAla: 4.281 ± 0.255
0.478IleCys: 0.478 ± 0.105
4.706IleAsp: 4.706 ± 0.289
3.68IleGlu: 3.68 ± 0.265
2.441IlePhe: 2.441 ± 0.214
4.069IleGly: 4.069 ± 0.367
0.69IleHis: 0.69 ± 0.123
3.538IleIle: 3.538 ± 0.269
3.874IleLys: 3.874 ± 0.276
4.281IleLeu: 4.281 ± 0.305
1.168IleMet: 1.168 ± 0.174
3.857IleAsn: 3.857 ± 0.305
2.848IlePro: 2.848 ± 0.263
2.229IleGln: 2.229 ± 0.268
2.53IleArg: 2.53 ± 0.21
4.034IleSer: 4.034 ± 0.378
5.148IleThr: 5.148 ± 0.661
3.91IleVal: 3.91 ± 0.429
0.655IleTrp: 0.655 ± 0.125
2.052IleTyr: 2.052 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
3.768LysAla: 3.768 ± 0.464
0.531LysCys: 0.531 ± 0.114
3.043LysAsp: 3.043 ± 0.292
3.786LysGlu: 3.786 ± 0.567
2.406LysPhe: 2.406 ± 0.259
3.556LysGly: 3.556 ± 0.371
0.778LysHis: 0.778 ± 0.187
3.91LysIle: 3.91 ± 0.252
4.388LysLys: 4.388 ± 0.661
4.6LysLeu: 4.6 ± 0.356
1.539LysMet: 1.539 ± 0.253
2.99LysAsn: 2.99 ± 0.289
2.229LysPro: 2.229 ± 0.29
2.176LysGln: 2.176 ± 0.297
2.406LysArg: 2.406 ± 0.288
3.945LysSer: 3.945 ± 0.329
4.087LysThr: 4.087 ± 0.243
4.158LysVal: 4.158 ± 0.341
0.796LysTrp: 0.796 ± 0.131
2.778LysTyr: 2.778 ± 0.293
0.0LysXaa: 0.0 ± 0.0
Leu
5.042LeuAla: 5.042 ± 0.309
0.69LeuCys: 0.69 ± 0.141
5.909LeuAsp: 5.909 ± 0.334
4.352LeuGlu: 4.352 ± 0.342
2.901LeuPhe: 2.901 ± 0.271
4.936LeuGly: 4.936 ± 0.347
1.256LeuHis: 1.256 ± 0.185
3.981LeuIle: 3.981 ± 0.303
4.741LeuLys: 4.741 ± 0.383
5.361LeuLeu: 5.361 ± 0.427
1.61LeuMet: 1.61 ± 0.244
4.352LeuAsn: 4.352 ± 0.242
3.149LeuPro: 3.149 ± 0.321
2.954LeuGln: 2.954 ± 0.212
3.308LeuArg: 3.308 ± 0.256
4.954LeuSer: 4.954 ± 0.323
5.767LeuThr: 5.767 ± 0.394
3.981LeuVal: 3.981 ± 0.251
0.584LeuTrp: 0.584 ± 0.112
2.884LeuTyr: 2.884 ± 0.212
0.0LeuXaa: 0.0 ± 0.0
Met
1.451MetAla: 1.451 ± 0.233
0.159MetCys: 0.159 ± 0.072
1.026MetAsp: 1.026 ± 0.179
1.486MetGlu: 1.486 ± 0.241
1.008MetPhe: 1.008 ± 0.167
1.327MetGly: 1.327 ± 0.253
0.389MetHis: 0.389 ± 0.112
1.256MetIle: 1.256 ± 0.214
1.946MetLys: 1.946 ± 0.33
1.716MetLeu: 1.716 ± 0.251
0.69MetMet: 0.69 ± 0.154
1.221MetAsn: 1.221 ± 0.182
0.885MetPro: 0.885 ± 0.139
1.044MetGln: 1.044 ± 0.154
1.132MetArg: 1.132 ± 0.191
1.521MetSer: 1.521 ± 0.246
1.645MetThr: 1.645 ± 0.235
1.079MetVal: 1.079 ± 0.174
0.265MetTrp: 0.265 ± 0.081
0.725MetTyr: 0.725 ± 0.129
0.0MetXaa: 0.0 ± 0.0
Asn
4.193AsnAla: 4.193 ± 0.291
0.389AsnCys: 0.389 ± 0.095
3.149AsnAsp: 3.149 ± 0.279
3.025AsnGlu: 3.025 ± 0.238
2.831AsnPhe: 2.831 ± 0.199
4.246AsnGly: 4.246 ± 0.521
0.725AsnHis: 0.725 ± 0.116
3.698AsnIle: 3.698 ± 0.325
2.848AsnLys: 2.848 ± 0.275
4.547AsnLeu: 4.547 ± 0.402
0.973AsnMet: 0.973 ± 0.159
3.485AsnAsn: 3.485 ± 0.246
3.255AsnPro: 3.255 ± 0.229
2.318AsnGln: 2.318 ± 0.198
2.406AsnArg: 2.406 ± 0.171
4.034AsnSer: 4.034 ± 0.278
4.246AsnThr: 4.246 ± 0.468
3.91AsnVal: 3.91 ± 0.332
0.708AsnTrp: 0.708 ± 0.107
2.282AsnTyr: 2.282 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
2.76ProAla: 2.76 ± 0.281
0.212ProCys: 0.212 ± 0.06
2.654ProAsp: 2.654 ± 0.232
2.778ProGlu: 2.778 ± 0.318
1.769ProPhe: 1.769 ± 0.208
3.344ProGly: 3.344 ± 0.319
0.725ProHis: 0.725 ± 0.108
2.318ProIle: 2.318 ± 0.271
2.229ProLys: 2.229 ± 0.319
2.601ProLeu: 2.601 ± 0.238
0.69ProMet: 0.69 ± 0.153
1.999ProAsn: 1.999 ± 0.221
1.716ProPro: 1.716 ± 0.193
1.097ProGln: 1.097 ± 0.122
1.645ProArg: 1.645 ± 0.161
3.114ProSer: 3.114 ± 0.209
2.831ProThr: 2.831 ± 0.223
2.53ProVal: 2.53 ± 0.253
0.584ProTrp: 0.584 ± 0.097
1.698ProTyr: 1.698 ± 0.177
0.0ProXaa: 0.0 ± 0.0
Gln
2.335GlnAla: 2.335 ± 0.203
0.336GlnCys: 0.336 ± 0.109
2.353GlnAsp: 2.353 ± 0.218
2.371GlnGlu: 2.371 ± 0.215
1.84GlnPhe: 1.84 ± 0.161
2.424GlnGly: 2.424 ± 0.216
0.708GlnHis: 0.708 ± 0.122
2.441GlnIle: 2.441 ± 0.273
2.424GlnLys: 2.424 ± 0.249
2.795GlnLeu: 2.795 ± 0.21
0.902GlnMet: 0.902 ± 0.156
2.088GlnAsn: 2.088 ± 0.18
1.309GlnPro: 1.309 ± 0.167
1.645GlnGln: 1.645 ± 0.27
1.592GlnArg: 1.592 ± 0.175
2.477GlnSer: 2.477 ± 0.188
2.725GlnThr: 2.725 ± 0.271
2.884GlnVal: 2.884 ± 0.227
0.495GlnTrp: 0.495 ± 0.109
1.893GlnTyr: 1.893 ± 0.214
0.0GlnXaa: 0.0 ± 0.0
Arg
2.512ArgAla: 2.512 ± 0.256
0.372ArgCys: 0.372 ± 0.079
2.406ArgAsp: 2.406 ± 0.207
2.565ArgGlu: 2.565 ± 0.31
2.035ArgPhe: 2.035 ± 0.16
3.096ArgGly: 3.096 ± 0.322
0.832ArgHis: 0.832 ± 0.147
3.043ArgIle: 3.043 ± 0.234
2.654ArgLys: 2.654 ± 0.347
3.538ArgLeu: 3.538 ± 0.275
1.221ArgMet: 1.221 ± 0.205
2.07ArgAsn: 2.07 ± 0.184
1.415ArgPro: 1.415 ± 0.14
1.628ArgGln: 1.628 ± 0.189
2.123ArgArg: 2.123 ± 0.328
2.229ArgSer: 2.229 ± 0.219
2.477ArgThr: 2.477 ± 0.262
2.831ArgVal: 2.831 ± 0.217
0.46ArgTrp: 0.46 ± 0.116
2.495ArgTyr: 2.495 ± 0.245
0.0ArgXaa: 0.0 ± 0.0
Ser
5.502SerAla: 5.502 ± 0.466
0.513SerCys: 0.513 ± 0.118
3.874SerAsp: 3.874 ± 0.34
3.379SerGlu: 3.379 ± 0.253
3.025SerPhe: 3.025 ± 0.238
7.572SerGly: 7.572 ± 0.77
0.832SerHis: 0.832 ± 0.104
4.087SerIle: 4.087 ± 0.411
3.468SerLys: 3.468 ± 0.334
5.077SerLeu: 5.077 ± 0.326
1.592SerMet: 1.592 ± 0.256
4.211SerAsn: 4.211 ± 0.35
2.353SerPro: 2.353 ± 0.238
2.406SerGln: 2.406 ± 0.222
2.53SerArg: 2.53 ± 0.246
5.661SerSer: 5.661 ± 0.515
5.272SerThr: 5.272 ± 0.486
4.936SerVal: 4.936 ± 0.431
0.725SerTrp: 0.725 ± 0.154
3.043SerTyr: 3.043 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
5.997ThrAla: 5.997 ± 0.734
0.407ThrCys: 0.407 ± 0.089
4.281ThrAsp: 4.281 ± 0.418
4.016ThrGlu: 4.016 ± 0.24
3.291ThrPhe: 3.291 ± 0.489
7.466ThrGly: 7.466 ± 0.836
0.885ThrHis: 0.885 ± 0.142
4.547ThrIle: 4.547 ± 0.373
3.715ThrLys: 3.715 ± 0.275
6.086ThrLeu: 6.086 ± 0.555
1.15ThrMet: 1.15 ± 0.15
4.511ThrAsn: 4.511 ± 0.566
2.972ThrPro: 2.972 ± 0.22
2.441ThrGln: 2.441 ± 0.231
2.813ThrArg: 2.813 ± 0.203
5.307ThrSer: 5.307 ± 0.602
6.281ThrThr: 6.281 ± 0.74
5.325ThrVal: 5.325 ± 0.634
0.814ThrTrp: 0.814 ± 0.114
2.689ThrTyr: 2.689 ± 0.23
0.0ThrXaa: 0.0 ± 0.0
Val
4.794ValAla: 4.794 ± 0.32
0.336ValCys: 0.336 ± 0.089
5.431ValAsp: 5.431 ± 0.453
4.423ValGlu: 4.423 ± 0.322
2.583ValPhe: 2.583 ± 0.238
5.113ValGly: 5.113 ± 0.427
0.92ValHis: 0.92 ± 0.142
3.998ValIle: 3.998 ± 0.441
3.928ValLys: 3.928 ± 0.31
3.874ValLeu: 3.874 ± 0.311
1.203ValMet: 1.203 ± 0.183
3.998ValAsn: 3.998 ± 0.303
2.654ValPro: 2.654 ± 0.263
2.424ValGln: 2.424 ± 0.205
2.654ValArg: 2.654 ± 0.256
4.83ValSer: 4.83 ± 0.263
5.52ValThr: 5.52 ± 0.657
4.228ValVal: 4.228 ± 0.342
0.655ValTrp: 0.655 ± 0.096
2.424ValTyr: 2.424 ± 0.239
0.0ValXaa: 0.0 ± 0.0
Trp
0.619TrpAla: 0.619 ± 0.113
0.124TrpCys: 0.124 ± 0.045
0.832TrpAsp: 0.832 ± 0.145
0.814TrpGlu: 0.814 ± 0.148
0.478TrpPhe: 0.478 ± 0.096
0.672TrpGly: 0.672 ± 0.126
0.318TrpHis: 0.318 ± 0.104
0.602TrpIle: 0.602 ± 0.11
0.885TrpLys: 0.885 ± 0.184
0.796TrpLeu: 0.796 ± 0.13
0.212TrpMet: 0.212 ± 0.075
0.743TrpAsn: 0.743 ± 0.116
0.248TrpPro: 0.248 ± 0.055
0.442TrpGln: 0.442 ± 0.094
0.513TrpArg: 0.513 ± 0.107
0.902TrpSer: 0.902 ± 0.119
0.743TrpThr: 0.743 ± 0.118
0.832TrpVal: 0.832 ± 0.101
0.142TrpTrp: 0.142 ± 0.052
0.495TrpTyr: 0.495 ± 0.086
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.707TyrAla: 2.707 ± 0.262
0.513TyrCys: 0.513 ± 0.12
3.397TyrAsp: 3.397 ± 0.265
2.459TyrGlu: 2.459 ± 0.309
1.875TyrPhe: 1.875 ± 0.181
2.583TyrGly: 2.583 ± 0.24
0.531TyrHis: 0.531 ± 0.112
2.654TyrIle: 2.654 ± 0.23
2.194TyrLys: 2.194 ± 0.227
2.866TyrLeu: 2.866 ± 0.222
0.955TyrMet: 0.955 ± 0.168
2.671TyrAsn: 2.671 ± 0.215
1.557TyrPro: 1.557 ± 0.177
1.698TyrGln: 1.698 ± 0.174
2.3TyrArg: 2.3 ± 0.256
2.548TyrSer: 2.548 ± 0.232
2.583TyrThr: 2.583 ± 0.372
3.008TyrVal: 3.008 ± 0.241
0.46TyrTrp: 0.46 ± 0.116
1.805TyrTyr: 1.805 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 215 proteins (56525 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski