Gulbenkiania indica
Average proteome isoelectric point is 6.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2697 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K6H6S7|A0A0K6H6S7_9NEIS Uncharacterized protein OS=Gulbenkiania indica OX=375574 GN=Ga0061063_2760 PE=4 SV=1
MM1 pKa = 7.36 NAPTEE6 pKa = 4.29 MPTPINFTNSACDD19 pKa = 3.37 KK20 pKa = 10.56 VRR22 pKa = 11.84 DD23 pKa = 4.4 LIAEE27 pKa = 4.23 EE28 pKa = 4.66 GNPDD32 pKa = 2.99 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.94 GFTFDD54 pKa = 4.65 EE55 pKa = 4.88 IANEE59 pKa = 4.13 DD60 pKa = 3.34 DD61 pKa = 3.58 TAIEE65 pKa = 4.16 RR66 pKa = 11.84 EE67 pKa = 4.44 GVTFLVDD74 pKa = 3.53 PMSYY78 pKa = 10.19 QYY80 pKa = 11.57 LVGAEE85 pKa = 3.7 IDD87 pKa = 3.88 YY88 pKa = 11.15 QEE90 pKa = 4.55 SLEE93 pKa = 4.16 GSQFVIRR100 pKa = 11.84 NPNAQTTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.859
Toseland 3.668
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.973
Patrickios 0.846
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A0K6GY63|A0A0K6GY63_9NEIS Biotin synthase OS=Gulbenkiania indica OX=375574 GN=bioB PE=3 SV=1
MM1 pKa = 8.13 RR2 pKa = 11.84 MLTPMRR8 pKa = 11.84 MLTPMRR14 pKa = 11.84 MLTPMRR20 pKa = 11.84 MLTPMPTLTLTRR32 pKa = 11.84 MLTPMLMPMPMLTRR46 pKa = 11.84 MLTRR50 pKa = 11.84 MPMLTRR56 pKa = 11.84 MPMLTPMPMLTPMLTLTLTPMLTPMLTPMPMPMPTPMLTPMPTLVV101 pKa = 3.46
Molecular weight: 11.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.485
IPC2_protein 11.067
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.32
Rodwell 12.34
Grimsley 12.881
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.106
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.163
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2697
0
2697
858314
34
1603
318.2
34.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.195 ± 0.065
0.925 ± 0.016
5.132 ± 0.034
5.953 ± 0.047
3.562 ± 0.035
8.012 ± 0.04
2.269 ± 0.023
4.33 ± 0.035
3.204 ± 0.043
11.425 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.467 ± 0.023
2.619 ± 0.027
5.202 ± 0.037
3.783 ± 0.034
7.195 ± 0.046
5.064 ± 0.036
5.158 ± 0.033
7.682 ± 0.044
1.386 ± 0.021
2.436 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here