Amino acid dipepetide frequency for Flavobacterium phage FPSV-S1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.534AlaAla: 6.534 ± 1.009
0.467AlaCys: 0.467 ± 0.245
3.174AlaAsp: 3.174 ± 0.634
3.08AlaGlu: 3.08 ± 0.633
3.36AlaPhe: 3.36 ± 0.485
5.787AlaGly: 5.787 ± 1.01
1.12AlaHis: 1.12 ± 0.344
4.947AlaIle: 4.947 ± 0.692
6.067AlaLys: 6.067 ± 0.727
6.721AlaLeu: 6.721 ± 0.88
1.4AlaMet: 1.4 ± 0.447
2.614AlaAsn: 2.614 ± 0.444
1.68AlaPro: 1.68 ± 0.432
2.894AlaGln: 2.894 ± 0.632
1.867AlaArg: 1.867 ± 0.473
4.761AlaSer: 4.761 ± 0.818
4.014AlaThr: 4.014 ± 0.676
5.041AlaVal: 5.041 ± 0.715
0.747AlaTrp: 0.747 ± 0.261
2.147AlaTyr: 2.147 ± 0.456
0.0AlaXaa: 0.0 ± 0.0
Cys
0.28CysAla: 0.28 ± 0.159
0.0CysCys: 0.0 ± 0.0
0.187CysAsp: 0.187 ± 0.133
0.747CysGlu: 0.747 ± 0.265
0.56CysPhe: 0.56 ± 0.226
0.84CysGly: 0.84 ± 0.269
0.0CysHis: 0.0 ± 0.0
1.12CysIle: 1.12 ± 0.291
0.56CysLys: 0.56 ± 0.204
0.747CysLeu: 0.747 ± 0.242
0.187CysMet: 0.187 ± 0.132
0.653CysAsn: 0.653 ± 0.249
0.187CysPro: 0.187 ± 0.124
0.373CysGln: 0.373 ± 0.152
0.187CysArg: 0.187 ± 0.137
0.28CysSer: 0.28 ± 0.211
0.187CysThr: 0.187 ± 0.129
0.373CysVal: 0.373 ± 0.223
0.28CysTrp: 0.28 ± 0.177
0.56CysTyr: 0.56 ± 0.209
0.0CysXaa: 0.0 ± 0.0
Asp
5.227AspAla: 5.227 ± 0.899
0.28AspCys: 0.28 ± 0.149
2.894AspAsp: 2.894 ± 0.575
5.041AspGlu: 5.041 ± 0.721
4.667AspPhe: 4.667 ± 0.641
4.574AspGly: 4.574 ± 0.702
0.747AspHis: 0.747 ± 0.263
5.787AspIle: 5.787 ± 0.865
6.161AspLys: 6.161 ± 0.642
5.694AspLeu: 5.694 ± 0.724
1.12AspMet: 1.12 ± 0.327
3.174AspAsn: 3.174 ± 0.511
2.52AspPro: 2.52 ± 0.374
1.12AspGln: 1.12 ± 0.332
1.027AspArg: 1.027 ± 0.287
2.054AspSer: 2.054 ± 0.417
3.734AspThr: 3.734 ± 0.638
3.64AspVal: 3.64 ± 0.526
1.12AspTrp: 1.12 ± 0.36
2.334AspTyr: 2.334 ± 0.531
0.0AspXaa: 0.0 ± 0.0
Glu
5.974GluAla: 5.974 ± 0.755
0.467GluCys: 0.467 ± 0.226
4.481GluAsp: 4.481 ± 0.689
4.481GluGlu: 4.481 ± 0.67
3.36GluPhe: 3.36 ± 0.585
3.547GluGly: 3.547 ± 0.485
0.56GluHis: 0.56 ± 0.196
7.001GluIle: 7.001 ± 0.902
5.227GluLys: 5.227 ± 0.928
5.507GluLeu: 5.507 ± 0.709
1.213GluMet: 1.213 ± 0.32
5.694GluAsn: 5.694 ± 0.691
1.027GluPro: 1.027 ± 0.321
1.867GluGln: 1.867 ± 0.322
2.24GluArg: 2.24 ± 0.457
2.427GluSer: 2.427 ± 0.442
3.454GluThr: 3.454 ± 0.388
5.227GluVal: 5.227 ± 0.579
0.653GluTrp: 0.653 ± 0.249
2.334GluTyr: 2.334 ± 0.359
0.0GluXaa: 0.0 ± 0.0
Phe
2.427PheAla: 2.427 ± 0.52
0.653PheCys: 0.653 ± 0.199
4.761PheAsp: 4.761 ± 0.536
3.547PheGlu: 3.547 ± 0.621
2.147PhePhe: 2.147 ± 0.455
3.174PheGly: 3.174 ± 0.682
0.467PheHis: 0.467 ± 0.229
3.92PheIle: 3.92 ± 0.637
4.947PheLys: 4.947 ± 0.864
4.667PheLeu: 4.667 ± 0.665
0.84PheMet: 0.84 ± 0.234
3.92PheAsn: 3.92 ± 0.596
1.027PhePro: 1.027 ± 0.294
1.12PheGln: 1.12 ± 0.264
1.774PheArg: 1.774 ± 0.389
3.92PheSer: 3.92 ± 0.57
3.08PheThr: 3.08 ± 0.635
2.334PheVal: 2.334 ± 0.398
0.467PheTrp: 0.467 ± 0.156
2.054PheTyr: 2.054 ± 0.448
0.0PheXaa: 0.0 ± 0.0
Gly
4.481GlyAla: 4.481 ± 1.017
0.56GlyCys: 0.56 ± 0.167
4.014GlyAsp: 4.014 ± 0.712
3.267GlyGlu: 3.267 ± 0.613
4.294GlyPhe: 4.294 ± 0.532
5.414GlyGly: 5.414 ± 1.302
0.747GlyHis: 0.747 ± 0.294
3.827GlyIle: 3.827 ± 0.432
4.854GlyLys: 4.854 ± 0.668
6.441GlyLeu: 6.441 ± 0.714
1.12GlyMet: 1.12 ± 0.349
2.427GlyAsn: 2.427 ± 0.512
0.093GlyPro: 0.093 ± 0.105
1.774GlyGln: 1.774 ± 0.446
1.867GlyArg: 1.867 ± 0.42
4.387GlySer: 4.387 ± 0.507
3.827GlyThr: 3.827 ± 0.619
5.601GlyVal: 5.601 ± 0.679
0.56GlyTrp: 0.56 ± 0.205
2.707GlyTyr: 2.707 ± 0.562
0.0GlyXaa: 0.0 ± 0.0
His
0.28HisAla: 0.28 ± 0.16
0.093HisCys: 0.093 ± 0.083
0.373HisAsp: 0.373 ± 0.155
0.56HisGlu: 0.56 ± 0.224
0.84HisPhe: 0.84 ± 0.297
0.747HisGly: 0.747 ± 0.276
0.0HisHis: 0.0 ± 0.0
0.747HisIle: 0.747 ± 0.314
0.747HisLys: 0.747 ± 0.225
1.12HisLeu: 1.12 ± 0.291
0.093HisMet: 0.093 ± 0.088
0.653HisAsn: 0.653 ± 0.264
0.56HisPro: 0.56 ± 0.205
0.28HisGln: 0.28 ± 0.136
0.373HisArg: 0.373 ± 0.254
0.467HisSer: 0.467 ± 0.189
0.373HisThr: 0.373 ± 0.156
0.933HisVal: 0.933 ± 0.287
0.373HisTrp: 0.373 ± 0.17
0.28HisTyr: 0.28 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
5.321IleAla: 5.321 ± 0.712
0.933IleCys: 0.933 ± 0.252
6.814IleAsp: 6.814 ± 0.874
7.374IleGlu: 7.374 ± 0.7
4.294IlePhe: 4.294 ± 0.515
4.294IleGly: 4.294 ± 0.914
0.373IleHis: 0.373 ± 0.153
5.227IleIle: 5.227 ± 0.65
8.588IleLys: 8.588 ± 0.983
7.094IleLeu: 7.094 ± 1.019
1.027IleMet: 1.027 ± 0.267
6.534IleAsn: 6.534 ± 0.671
2.707IlePro: 2.707 ± 0.462
2.707IleGln: 2.707 ± 0.474
2.614IleArg: 2.614 ± 0.525
5.227IleSer: 5.227 ± 0.514
4.387IleThr: 4.387 ± 0.63
4.014IleVal: 4.014 ± 0.597
0.56IleTrp: 0.56 ± 0.251
2.614IleTyr: 2.614 ± 0.608
0.0IleXaa: 0.0 ± 0.0
Lys
6.814LysAla: 6.814 ± 1.027
0.84LysCys: 0.84 ± 0.25
6.441LysAsp: 6.441 ± 0.701
7.188LysGlu: 7.188 ± 0.959
3.64LysPhe: 3.64 ± 0.513
4.667LysGly: 4.667 ± 0.783
1.12LysHis: 1.12 ± 0.308
8.774LysIle: 8.774 ± 0.905
9.148LysLys: 9.148 ± 1.121
5.134LysLeu: 5.134 ± 0.636
1.774LysMet: 1.774 ± 0.354
5.787LysAsn: 5.787 ± 0.727
2.8LysPro: 2.8 ± 0.638
3.64LysGln: 3.64 ± 0.511
4.014LysArg: 4.014 ± 0.59
4.014LysSer: 4.014 ± 0.686
6.627LysThr: 6.627 ± 0.853
4.761LysVal: 4.761 ± 0.599
0.84LysTrp: 0.84 ± 0.228
2.894LysTyr: 2.894 ± 0.404
0.0LysXaa: 0.0 ± 0.0
Leu
3.92LeuAla: 3.92 ± 0.578
0.56LeuCys: 0.56 ± 0.258
5.227LeuAsp: 5.227 ± 0.769
6.161LeuGlu: 6.161 ± 0.673
3.454LeuPhe: 3.454 ± 0.569
5.041LeuGly: 5.041 ± 0.685
1.213LeuHis: 1.213 ± 0.265
7.001LeuIle: 7.001 ± 0.926
8.121LeuLys: 8.121 ± 0.883
7.748LeuLeu: 7.748 ± 0.776
1.4LeuMet: 1.4 ± 0.354
6.067LeuAsn: 6.067 ± 0.852
4.107LeuPro: 4.107 ± 0.669
4.761LeuGln: 4.761 ± 0.604
3.454LeuArg: 3.454 ± 0.652
5.601LeuSer: 5.601 ± 0.637
5.787LeuThr: 5.787 ± 0.61
4.387LeuVal: 4.387 ± 0.493
0.84LeuTrp: 0.84 ± 0.289
3.454LeuTyr: 3.454 ± 0.463
0.0LeuXaa: 0.0 ± 0.0
Met
1.494MetAla: 1.494 ± 0.28
0.093MetCys: 0.093 ± 0.095
0.933MetAsp: 0.933 ± 0.254
0.56MetGlu: 0.56 ± 0.234
0.747MetPhe: 0.747 ± 0.298
1.027MetGly: 1.027 ± 0.31
0.093MetHis: 0.093 ± 0.096
1.027MetIle: 1.027 ± 0.324
1.774MetLys: 1.774 ± 0.474
1.494MetLeu: 1.494 ± 0.418
0.093MetMet: 0.093 ± 0.094
0.84MetAsn: 0.84 ± 0.273
0.28MetPro: 0.28 ± 0.16
0.84MetGln: 0.84 ± 0.258
0.56MetArg: 0.56 ± 0.245
1.68MetSer: 1.68 ± 0.421
0.747MetThr: 0.747 ± 0.227
1.12MetVal: 1.12 ± 0.374
0.093MetTrp: 0.093 ± 0.091
0.56MetTyr: 0.56 ± 0.239
0.0MetXaa: 0.0 ± 0.0
Asn
3.92AsnAla: 3.92 ± 0.573
0.56AsnCys: 0.56 ± 0.206
4.481AsnAsp: 4.481 ± 0.662
3.64AsnGlu: 3.64 ± 0.551
4.014AsnPhe: 4.014 ± 0.735
4.481AsnGly: 4.481 ± 0.623
0.653AsnHis: 0.653 ± 0.221
5.134AsnIle: 5.134 ± 0.586
5.041AsnLys: 5.041 ± 0.588
5.041AsnLeu: 5.041 ± 0.652
1.4AsnMet: 1.4 ± 0.403
4.201AsnAsn: 4.201 ± 0.769
2.334AsnPro: 2.334 ± 0.529
2.054AsnGln: 2.054 ± 0.482
2.334AsnArg: 2.334 ± 0.492
3.267AsnSer: 3.267 ± 0.458
3.08AsnThr: 3.08 ± 0.456
5.414AsnVal: 5.414 ± 0.83
0.84AsnTrp: 0.84 ± 0.221
2.894AsnTyr: 2.894 ± 0.631
0.0AsnXaa: 0.0 ± 0.0
Pro
2.147ProAla: 2.147 ± 0.496
0.28ProCys: 0.28 ± 0.147
1.774ProAsp: 1.774 ± 0.4
2.52ProGlu: 2.52 ± 0.498
1.307ProPhe: 1.307 ± 0.379
0.0ProGly: 0.0 ± 0.0
0.373ProHis: 0.373 ± 0.18
2.054ProIle: 2.054 ± 0.384
3.174ProLys: 3.174 ± 0.479
2.24ProLeu: 2.24 ± 0.601
0.373ProMet: 0.373 ± 0.176
2.427ProAsn: 2.427 ± 0.449
1.027ProPro: 1.027 ± 0.338
1.027ProGln: 1.027 ± 0.247
1.4ProArg: 1.4 ± 0.405
1.587ProSer: 1.587 ± 0.364
1.587ProThr: 1.587 ± 0.399
2.334ProVal: 2.334 ± 0.401
0.373ProTrp: 0.373 ± 0.162
0.933ProTyr: 0.933 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
2.334GlnAla: 2.334 ± 0.485
0.373GlnCys: 0.373 ± 0.15
2.52GlnAsp: 2.52 ± 0.456
1.587GlnGlu: 1.587 ± 0.339
1.307GlnPhe: 1.307 ± 0.348
1.774GlnGly: 1.774 ± 0.339
0.28GlnHis: 0.28 ± 0.158
3.734GlnIle: 3.734 ± 0.525
2.52GlnLys: 2.52 ± 0.489
2.894GlnLeu: 2.894 ± 0.509
1.12GlnMet: 1.12 ± 0.256
3.454GlnAsn: 3.454 ± 0.702
0.84GlnPro: 0.84 ± 0.239
1.027GlnGln: 1.027 ± 0.248
2.054GlnArg: 2.054 ± 0.446
2.054GlnSer: 2.054 ± 0.44
2.427GlnThr: 2.427 ± 0.431
2.054GlnVal: 2.054 ± 0.522
0.373GlnTrp: 0.373 ± 0.14
1.68GlnTyr: 1.68 ± 0.427
0.0GlnXaa: 0.0 ± 0.0
Arg
1.774ArgAla: 1.774 ± 0.333
0.56ArgCys: 0.56 ± 0.272
2.614ArgAsp: 2.614 ± 0.503
2.427ArgGlu: 2.427 ± 0.65
1.867ArgPhe: 1.867 ± 0.417
1.96ArgGly: 1.96 ± 0.433
0.093ArgHis: 0.093 ± 0.094
2.987ArgIle: 2.987 ± 0.52
2.707ArgLys: 2.707 ± 0.512
2.894ArgLeu: 2.894 ± 0.66
0.56ArgMet: 0.56 ± 0.284
2.147ArgAsn: 2.147 ± 0.468
1.494ArgPro: 1.494 ± 0.367
2.054ArgGln: 2.054 ± 0.408
1.68ArgArg: 1.68 ± 0.378
1.213ArgSer: 1.213 ± 0.361
1.587ArgThr: 1.587 ± 0.342
2.427ArgVal: 2.427 ± 0.504
0.467ArgTrp: 0.467 ± 0.229
1.587ArgTyr: 1.587 ± 0.42
0.0ArgXaa: 0.0 ± 0.0
Ser
4.201SerAla: 4.201 ± 0.689
0.467SerCys: 0.467 ± 0.235
2.614SerAsp: 2.614 ± 0.462
3.36SerGlu: 3.36 ± 0.524
2.987SerPhe: 2.987 ± 0.674
4.201SerGly: 4.201 ± 0.56
0.187SerHis: 0.187 ± 0.153
4.201SerIle: 4.201 ± 0.578
4.667SerLys: 4.667 ± 0.498
5.881SerLeu: 5.881 ± 0.472
0.84SerMet: 0.84 ± 0.262
3.08SerAsn: 3.08 ± 0.509
1.494SerPro: 1.494 ± 0.344
2.334SerGln: 2.334 ± 0.4
2.427SerArg: 2.427 ± 0.463
2.894SerSer: 2.894 ± 0.574
2.894SerThr: 2.894 ± 0.466
4.667SerVal: 4.667 ± 0.787
0.933SerTrp: 0.933 ± 0.218
1.96SerTyr: 1.96 ± 0.441
0.0SerXaa: 0.0 ± 0.0
Thr
5.694ThrAla: 5.694 ± 0.908
0.373ThrCys: 0.373 ± 0.183
3.827ThrAsp: 3.827 ± 0.59
4.107ThrGlu: 4.107 ± 0.574
2.147ThrPhe: 2.147 ± 0.382
3.734ThrGly: 3.734 ± 0.447
0.467ThrHis: 0.467 ± 0.248
5.414ThrIle: 5.414 ± 0.825
5.414ThrLys: 5.414 ± 0.772
5.227ThrLeu: 5.227 ± 0.729
0.373ThrMet: 0.373 ± 0.181
3.454ThrAsn: 3.454 ± 0.533
1.96ThrPro: 1.96 ± 0.453
2.8ThrGln: 2.8 ± 0.448
1.587ThrArg: 1.587 ± 0.388
3.36ThrSer: 3.36 ± 0.654
2.52ThrThr: 2.52 ± 0.385
3.36ThrVal: 3.36 ± 0.576
0.28ThrTrp: 0.28 ± 0.139
2.427ThrTyr: 2.427 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
3.454ValAla: 3.454 ± 0.577
0.187ValCys: 0.187 ± 0.125
2.334ValAsp: 2.334 ± 0.47
4.387ValGlu: 4.387 ± 0.568
3.454ValPhe: 3.454 ± 0.6
3.827ValGly: 3.827 ± 0.581
0.933ValHis: 0.933 ± 0.264
6.627ValIle: 6.627 ± 0.827
5.974ValLys: 5.974 ± 0.709
5.881ValLeu: 5.881 ± 0.759
0.56ValMet: 0.56 ± 0.228
4.294ValAsn: 4.294 ± 0.58
1.494ValPro: 1.494 ± 0.383
2.24ValGln: 2.24 ± 0.454
2.054ValArg: 2.054 ± 0.405
4.014ValSer: 4.014 ± 0.683
5.227ValThr: 5.227 ± 0.75
3.734ValVal: 3.734 ± 0.505
1.12ValTrp: 1.12 ± 0.337
2.334ValTyr: 2.334 ± 0.385
0.0ValXaa: 0.0 ± 0.0
Trp
0.467TrpAla: 0.467 ± 0.172
0.187TrpCys: 0.187 ± 0.122
1.027TrpAsp: 1.027 ± 0.291
0.56TrpGlu: 0.56 ± 0.239
0.467TrpPhe: 0.467 ± 0.203
0.84TrpGly: 0.84 ± 0.3
0.0TrpHis: 0.0 ± 0.0
0.747TrpIle: 0.747 ± 0.215
0.653TrpLys: 0.653 ± 0.237
1.96TrpLeu: 1.96 ± 0.393
0.0TrpMet: 0.0 ± 0.0
0.467TrpAsn: 0.467 ± 0.197
0.0TrpPro: 0.0 ± 0.0
0.56TrpGln: 0.56 ± 0.224
0.56TrpArg: 0.56 ± 0.183
0.84TrpSer: 0.84 ± 0.326
0.653TrpThr: 0.653 ± 0.264
1.307TrpVal: 1.307 ± 0.306
0.0TrpTrp: 0.0 ± 0.0
0.093TrpTyr: 0.093 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.68TyrAla: 1.68 ± 0.35
0.373TyrCys: 0.373 ± 0.181
2.427TyrAsp: 2.427 ± 0.479
2.24TyrGlu: 2.24 ± 0.411
2.334TyrPhe: 2.334 ± 0.491
2.334TyrGly: 2.334 ± 0.447
0.467TyrHis: 0.467 ± 0.21
2.427TyrIle: 2.427 ± 0.646
4.574TyrLys: 4.574 ± 0.579
3.92TyrLeu: 3.92 ± 0.627
0.467TyrMet: 0.467 ± 0.163
2.894TyrAsn: 2.894 ± 0.536
1.307TyrPro: 1.307 ± 0.296
0.933TyrGln: 0.933 ± 0.349
1.027TyrArg: 1.027 ± 0.291
2.24TyrSer: 2.24 ± 0.404
2.334TyrThr: 2.334 ± 0.426
1.4TyrVal: 1.4 ± 0.318
0.467TyrTrp: 0.467 ± 0.196
0.84TyrTyr: 0.84 ± 0.317
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (10714 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski