Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Thermococcus; Thermococcus kodakarensis

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q5JHL8|PSB2_THEKO Proteasome subunit beta 2 OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=psmB2 PE=3 SV=1
MM1 pKa = 7.62SKK3 pKa = 9.19FTGLLLILILLGAAYY18 pKa = 9.31IYY20 pKa = 7.64YY21 pKa = 8.47TNPHH25 pKa = 6.83IIEE28 pKa = 5.5DD29 pKa = 3.87LTKK32 pKa = 10.45MKK34 pKa = 10.81DD35 pKa = 3.24SFPNGFDD42 pKa = 3.21SGGTTTHH49 pKa = 6.52SSGGSGFSEE58 pKa = 4.23TSSGNHH64 pKa = 5.44SEE66 pKa = 4.19SFPGEE71 pKa = 4.25KK72 pKa = 10.22GWVDD76 pKa = 3.21SDD78 pKa = 3.37NDD80 pKa = 3.5GRR82 pKa = 11.84KK83 pKa = 9.5DD84 pKa = 3.54VYY86 pKa = 10.9IGDD89 pKa = 4.83DD90 pKa = 3.62YY91 pKa = 12.23AEE93 pKa = 4.22VPTTNGSVLFVDD105 pKa = 4.19RR106 pKa = 11.84NKK108 pKa = 10.83DD109 pKa = 3.38GKK111 pKa = 10.4IDD113 pKa = 5.02AIYY116 pKa = 10.87LDD118 pKa = 3.83TTGDD122 pKa = 3.43GSYY125 pKa = 9.6DD126 pKa = 3.31TAYY129 pKa = 11.05LDD131 pKa = 3.51EE132 pKa = 5.91DD133 pKa = 4.81YY134 pKa = 11.37NGKK137 pKa = 8.12TDD139 pKa = 3.1TWRR142 pKa = 11.84TTFNGVDD149 pKa = 3.36SYY151 pKa = 12.18AWDD154 pKa = 3.36ITGDD158 pKa = 4.56GIPDD162 pKa = 3.59VYY164 pKa = 11.21DD165 pKa = 3.55SNGDD169 pKa = 3.69GKK171 pKa = 11.13VDD173 pKa = 3.23AWDD176 pKa = 3.86TNSDD180 pKa = 3.97GIIDD184 pKa = 4.24EE185 pKa = 4.78RR186 pKa = 11.84DD187 pKa = 2.87VDD189 pKa = 4.15YY190 pKa = 11.51DD191 pKa = 3.76GTPDD195 pKa = 3.23LHH197 pKa = 8.36DD198 pKa = 3.83YY199 pKa = 11.48DD200 pKa = 4.44FDD202 pKa = 4.2GVFDD206 pKa = 3.72EE207 pKa = 5.16FEE209 pKa = 4.97RR210 pKa = 11.84NVTLYY215 pKa = 10.23PPEE218 pKa = 4.4SANGTSGAFLCPDD231 pKa = 3.99NKK233 pKa = 10.86DD234 pKa = 3.01DD235 pKa = 4.38AYY237 pKa = 11.41RR238 pKa = 11.84LFVQAYY244 pKa = 9.11NNVTSLQSSGASDD257 pKa = 4.21EE258 pKa = 4.93EE259 pKa = 4.1IQEE262 pKa = 4.82AYY264 pKa = 10.35AKK266 pKa = 8.71YY267 pKa = 9.87QKK269 pKa = 10.83ARR271 pKa = 11.84ACYY274 pKa = 9.64EE275 pKa = 3.98SFLTSTTSAASSSGMRR291 pKa = 11.84EE292 pKa = 3.8IKK294 pKa = 9.63TVTLDD299 pKa = 3.46TEE301 pKa = 4.27GTAGVKK307 pKa = 10.58FSTGEE312 pKa = 3.66LKK314 pKa = 10.61GYY316 pKa = 10.44NEE318 pKa = 3.93ISDD321 pKa = 4.34NVWEE325 pKa = 4.73DD326 pKa = 2.91VDD328 pKa = 5.49LVAEE332 pKa = 4.78PWCVEE337 pKa = 3.81YY338 pKa = 9.39PALLGHH344 pKa = 7.24WIDD347 pKa = 4.2LGEE350 pKa = 4.56GNLDD354 pKa = 3.48TLDD357 pKa = 3.86PSEE360 pKa = 5.16IPTSGYY366 pKa = 6.73PTGEE370 pKa = 3.63IAEE373 pKa = 4.55EE374 pKa = 3.67IKK376 pKa = 10.28MGHH379 pKa = 6.1VYY381 pKa = 10.92VNLNSDD387 pKa = 3.59GTLTAFEE394 pKa = 4.63LVSHH398 pKa = 6.96EE399 pKa = 4.12KK400 pKa = 10.13TGDD403 pKa = 3.29CSHH406 pKa = 7.53RR407 pKa = 11.84ITIRR411 pKa = 11.84YY412 pKa = 9.04SNLGGSS418 pKa = 3.77

Molecular weight: 45.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5JEZ6|Q5JEZ6_THEKO Non-specific serine/threonine protein kinase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=TK0679 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.23RR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84KK7 pKa = 7.59WKK9 pKa = 10.02KK10 pKa = 8.58KK11 pKa = 10.27GRR13 pKa = 11.84MRR15 pKa = 11.84WKK17 pKa = 9.51WIKK20 pKa = 10.46KK21 pKa = 8.96RR22 pKa = 11.84IRR24 pKa = 11.84RR25 pKa = 11.84LKK27 pKa = 8.25RR28 pKa = 11.84QRR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 10.18EE33 pKa = 3.73RR34 pKa = 11.84GLII37 pKa = 3.73

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2301

0

2301

637680

33

1798

277.1

31.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.419 ± 0.052

0.531 ± 0.016

4.733 ± 0.041

8.826 ± 0.078

4.33 ± 0.039

7.584 ± 0.052

1.592 ± 0.019

6.961 ± 0.049

6.868 ± 0.055

10.472 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.023

3.214 ± 0.034

4.388 ± 0.03

1.823 ± 0.027

5.84 ± 0.05

5.047 ± 0.043

4.615 ± 0.042

8.292 ± 0.043

1.296 ± 0.02

3.832 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski