Escherichia phage ID32

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Alphatrevirus; Escherichia virus ID32

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2LMA9|Q2LMA9_9VIRU GpC OS=Escherichia phage ID32 OX=338102 GN=C PE=4 SV=1
MM1 pKa = 7.44SNLVATDD8 pKa = 3.45VNFATSVAALKK19 pKa = 9.45MLQASAVLDD28 pKa = 3.38ITEE31 pKa = 4.35EE32 pKa = 4.21DD33 pKa = 3.65FDD35 pKa = 5.19FLTGDD40 pKa = 4.49KK41 pKa = 10.3IWIATDD47 pKa = 3.1RR48 pKa = 11.84NRR50 pKa = 11.84ARR52 pKa = 11.84RR53 pKa = 11.84CVEE56 pKa = 3.39ACVYY60 pKa = 8.38GTLDD64 pKa = 3.27FVGYY68 pKa = 9.24PRR70 pKa = 11.84FPAPVEE76 pKa = 4.43FIAAVIAYY84 pKa = 7.7YY85 pKa = 8.79VHH87 pKa = 6.96PVNIQTACLIMEE99 pKa = 4.57GAEE102 pKa = 3.88FTEE105 pKa = 4.67NIVNGVEE112 pKa = 4.24RR113 pKa = 11.84PVKK116 pKa = 10.06AAEE119 pKa = 3.93LFAYY123 pKa = 7.45TLRR126 pKa = 11.84VKK128 pKa = 10.74AGFKK132 pKa = 9.24EE133 pKa = 4.59TVLSAEE139 pKa = 3.87EE140 pKa = 3.75NARR143 pKa = 11.84QKK145 pKa = 10.81LRR147 pKa = 11.84ASGVMM152 pKa = 3.55

Molecular weight: 16.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2LMA7|Q2LMA7_9VIRU GPE OS=Escherichia phage ID32 OX=338102 GN=E PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.16KK3 pKa = 10.29ARR5 pKa = 11.84RR6 pKa = 11.84SPSRR10 pKa = 11.84KK11 pKa = 8.81KK12 pKa = 10.43GARR15 pKa = 11.84LWYY18 pKa = 10.44VGGSQFF24 pKa = 4.73

Molecular weight: 2.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

1917

24

494

191.7

21.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.181 ± 1.482

1.043 ± 0.243

5.686 ± 0.527

4.851 ± 0.705

4.538 ± 0.827

5.686 ± 1.09

2.191 ± 0.526

3.912 ± 0.379

5.738 ± 1.185

8.555 ± 1.73

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.547 ± 0.362

4.434 ± 0.595

4.225 ± 0.778

5.477 ± 1.026

6.155 ± 0.772

7.825 ± 1.083

5.477 ± 0.34

6.677 ± 1.411

1.565 ± 0.415

3.234 ± 0.601
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski