Amino acid dipepetide frequency for Escherichia phage ID32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.916AlaAla: 9.916 ± 3.705
2.088AlaCys: 2.088 ± 1.353
2.61AlaAsp: 2.61 ± 0.671
5.741AlaGlu: 5.741 ± 2.096
3.132AlaPhe: 3.132 ± 1.264
8.351AlaGly: 8.351 ± 4.406
1.566AlaHis: 1.566 ± 0.758
3.132AlaIle: 3.132 ± 0.765
5.219AlaLys: 5.219 ± 2.245
8.351AlaLeu: 8.351 ± 1.487
0.522AlaMet: 0.522 ± 0.406
2.61AlaAsn: 2.61 ± 1.33
5.219AlaPro: 5.219 ± 2.188
4.697AlaGln: 4.697 ± 1.306
4.697AlaArg: 4.697 ± 2.429
8.873AlaSer: 8.873 ± 2.474
7.307AlaThr: 7.307 ± 2.064
4.697AlaVal: 4.697 ± 1.571
1.044AlaTrp: 1.044 ± 0.56
2.088AlaTyr: 2.088 ± 1.12
0.0AlaXaa: 0.0 ± 0.0
Cys
1.044CysAla: 1.044 ± 0.786
0.0CysCys: 0.0 ± 0.0
0.522CysAsp: 0.522 ± 0.463
0.522CysGlu: 0.522 ± 0.69
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
0.522CysLys: 0.522 ± 0.406
1.044CysLeu: 1.044 ± 0.659
0.0CysMet: 0.0 ± 0.0
0.522CysAsn: 0.522 ± 0.463
1.044CysPro: 1.044 ± 0.479
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
0.522CysSer: 0.522 ± 0.463
0.522CysThr: 0.522 ± 0.463
3.653CysVal: 3.653 ± 1.814
0.0CysTrp: 0.0 ± 0.0
0.522CysTyr: 0.522 ± 0.406
0.0CysXaa: 0.0 ± 0.0
Asp
4.697AspAla: 4.697 ± 1.506
1.044AspCys: 1.044 ± 0.68
3.132AspAsp: 3.132 ± 1.744
3.132AspGlu: 3.132 ± 1.224
3.132AspPhe: 3.132 ± 2.327
3.132AspGly: 3.132 ± 1.597
1.566AspHis: 1.566 ± 0.937
3.653AspIle: 3.653 ± 1.108
1.566AspLys: 1.566 ± 0.706
5.741AspLeu: 5.741 ± 1.796
1.566AspMet: 1.566 ± 0.665
2.61AspAsn: 2.61 ± 0.842
2.088AspPro: 2.088 ± 0.849
2.088AspGln: 2.088 ± 0.885
2.088AspArg: 2.088 ± 0.556
6.263AspSer: 6.263 ± 1.489
2.61AspThr: 2.61 ± 0.957
3.653AspVal: 3.653 ± 0.729
1.044AspTrp: 1.044 ± 0.812
2.088AspTyr: 2.088 ± 0.697
0.0AspXaa: 0.0 ± 0.0
Glu
2.088GluAla: 2.088 ± 1.083
0.522GluCys: 0.522 ± 0.406
2.61GluAsp: 2.61 ± 0.923
1.566GluGlu: 1.566 ± 1.203
4.697GluPhe: 4.697 ± 1.364
2.61GluGly: 2.61 ± 0.993
1.044GluHis: 1.044 ± 0.778
1.566GluIle: 1.566 ± 1.018
5.741GluLys: 5.741 ± 3.352
5.741GluLeu: 5.741 ± 2.17
2.61GluMet: 2.61 ± 0.842
2.088GluAsn: 2.088 ± 1.318
0.522GluPro: 0.522 ± 0.757
1.044GluGln: 1.044 ± 0.926
4.175GluArg: 4.175 ± 1.533
3.653GluSer: 3.653 ± 1.839
3.653GluThr: 3.653 ± 1.027
2.61GluVal: 2.61 ± 1.011
0.0GluTrp: 0.0 ± 0.0
0.0GluTyr: 0.0 ± 0.0
0.0GluXaa: 0.0 ± 0.0
Phe
1.566PheAla: 1.566 ± 1.44
0.522PheCys: 0.522 ± 0.463
4.175PheAsp: 4.175 ± 1.325
1.566PheGlu: 1.566 ± 0.685
2.61PhePhe: 2.61 ± 0.788
4.175PheGly: 4.175 ± 1.037
1.044PheHis: 1.044 ± 0.778
1.566PheIle: 1.566 ± 1.087
3.132PheLys: 3.132 ± 1.102
2.61PheLeu: 2.61 ± 0.975
2.61PheMet: 2.61 ± 1.365
1.566PheAsn: 1.566 ± 0.758
2.088PhePro: 2.088 ± 0.942
2.088PheGln: 2.088 ± 1.126
2.61PheArg: 2.61 ± 1.329
4.175PheSer: 4.175 ± 3.052
3.132PheThr: 3.132 ± 1.002
2.088PheVal: 2.088 ± 1.385
0.522PheTrp: 0.522 ± 0.463
1.566PheTyr: 1.566 ± 1.033
0.0PheXaa: 0.0 ± 0.0
Gly
6.263GlyAla: 6.263 ± 2.515
0.522GlyCys: 0.522 ± 0.757
1.566GlyAsp: 1.566 ± 0.609
1.566GlyGlu: 1.566 ± 1.056
2.088GlyPhe: 2.088 ± 0.825
5.219GlyGly: 5.219 ± 2.973
0.522GlyHis: 0.522 ± 0.757
3.132GlyIle: 3.132 ± 1.971
4.697GlyLys: 4.697 ± 1.798
2.61GlyLeu: 2.61 ± 1.011
1.566GlyMet: 1.566 ± 0.838
3.653GlyAsn: 3.653 ± 1.926
0.0GlyPro: 0.0 ± 0.0
3.653GlyGln: 3.653 ± 1.831
3.653GlyArg: 3.653 ± 1.498
4.697GlySer: 4.697 ± 1.421
1.566GlyThr: 1.566 ± 0.986
6.263GlyVal: 6.263 ± 1.721
1.566GlyTrp: 1.566 ± 0.758
2.088GlyTyr: 2.088 ± 1.091
0.0GlyXaa: 0.0 ± 0.0
His
1.044HisAla: 1.044 ± 0.724
0.0HisCys: 0.0 ± 0.0
2.088HisAsp: 2.088 ± 0.852
1.566HisGlu: 1.566 ± 0.693
0.522HisPhe: 0.522 ± 0.757
1.044HisGly: 1.044 ± 0.479
0.0HisHis: 0.0 ± 0.0
0.0HisIle: 0.0 ± 0.0
0.522HisLys: 0.522 ± 0.605
3.132HisLeu: 3.132 ± 0.999
1.044HisMet: 1.044 ± 0.811
0.0HisAsn: 0.0 ± 0.0
1.044HisPro: 1.044 ± 0.659
1.566HisGln: 1.566 ± 0.502
1.566HisArg: 1.566 ± 0.708
2.088HisSer: 2.088 ± 1.851
0.522HisThr: 0.522 ± 0.463
2.61HisVal: 2.61 ± 0.933
1.044HisTrp: 1.044 ± 0.479
0.522HisTyr: 0.522 ± 0.463
0.0HisXaa: 0.0 ± 0.0
Ile
6.785IleAla: 6.785 ± 2.241
1.566IleCys: 1.566 ± 0.949
2.61IleAsp: 2.61 ± 0.741
1.044IleGlu: 1.044 ± 0.717
0.0IlePhe: 0.0 ± 0.0
3.132IleGly: 3.132 ± 0.921
1.044IleHis: 1.044 ± 0.83
0.522IleIle: 0.522 ± 0.406
1.044IleLys: 1.044 ± 0.584
1.044IleLeu: 1.044 ± 0.56
3.653IleMet: 3.653 ± 1.356
1.044IleAsn: 1.044 ± 0.811
4.175IlePro: 4.175 ± 1.612
2.61IleGln: 2.61 ± 1.643
1.566IleArg: 1.566 ± 0.758
1.566IleSer: 1.566 ± 0.838
1.566IleThr: 1.566 ± 0.909
2.088IleVal: 2.088 ± 0.825
1.044IleTrp: 1.044 ± 0.676
1.044IleTyr: 1.044 ± 0.926
0.0IleXaa: 0.0 ± 0.0
Lys
5.741LysAla: 5.741 ± 1.771
1.044LysCys: 1.044 ± 0.811
4.175LysAsp: 4.175 ± 2.413
2.61LysGlu: 2.61 ± 0.669
1.566LysPhe: 1.566 ± 1.389
2.61LysGly: 2.61 ± 1.366
2.088LysHis: 2.088 ± 0.849
2.61LysIle: 2.61 ± 1.123
5.741LysLys: 5.741 ± 2.495
4.175LysLeu: 4.175 ± 1.84
2.61LysMet: 2.61 ± 1.095
2.61LysAsn: 2.61 ± 1.571
2.61LysPro: 2.61 ± 0.932
3.653LysGln: 3.653 ± 1.054
2.61LysArg: 2.61 ± 1.198
0.522LysSer: 0.522 ± 0.504
3.132LysThr: 3.132 ± 1.543
3.132LysVal: 3.132 ± 1.062
1.044LysTrp: 1.044 ± 1.082
2.61LysTyr: 2.61 ± 1.748
0.0LysXaa: 0.0 ± 0.0
Leu
6.263LeuAla: 6.263 ± 2.422
0.522LeuCys: 0.522 ± 0.406
7.307LeuAsp: 7.307 ± 1.504
3.132LeuGlu: 3.132 ± 1.376
2.61LeuPhe: 2.61 ± 0.669
2.61LeuGly: 2.61 ± 0.958
2.088LeuHis: 2.088 ± 0.958
2.61LeuIle: 2.61 ± 1.173
6.785LeuLys: 6.785 ± 1.805
8.873LeuLeu: 8.873 ± 5.296
3.653LeuMet: 3.653 ± 1.034
3.132LeuAsn: 3.132 ± 1.042
3.653LeuPro: 3.653 ± 1.486
3.132LeuGln: 3.132 ± 0.842
6.785LeuArg: 6.785 ± 2.605
9.916LeuSer: 9.916 ± 2.664
5.219LeuThr: 5.219 ± 1.57
4.175LeuVal: 4.175 ± 2.013
2.088LeuTrp: 2.088 ± 1.176
1.044LeuTyr: 1.044 ± 0.811
0.0LeuXaa: 0.0 ± 0.0
Met
5.219MetAla: 5.219 ± 1.618
0.0MetCys: 0.0 ± 0.0
1.566MetAsp: 1.566 ± 0.693
3.653MetGlu: 3.653 ± 1.297
2.088MetPhe: 2.088 ± 1.06
0.522MetGly: 0.522 ± 0.463
1.044MetHis: 1.044 ± 0.926
2.088MetIle: 2.088 ± 0.885
2.088MetLys: 2.088 ± 0.912
2.61MetLeu: 2.61 ± 1.183
1.044MetMet: 1.044 ± 0.873
0.0MetAsn: 0.0 ± 0.0
0.522MetPro: 0.522 ± 0.463
5.219MetGln: 5.219 ± 1.282
1.566MetArg: 1.566 ± 0.985
2.088MetSer: 2.088 ± 0.942
2.61MetThr: 2.61 ± 0.842
1.044MetVal: 1.044 ± 0.811
0.522MetTrp: 0.522 ± 0.406
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
4.697AsnAla: 4.697 ± 1.87
0.522AsnCys: 0.522 ± 0.463
1.044AsnAsp: 1.044 ± 0.479
2.088AsnGlu: 2.088 ± 0.701
1.044AsnPhe: 1.044 ± 0.659
3.653AsnGly: 3.653 ± 1.44
0.522AsnHis: 0.522 ± 0.605
2.61AsnIle: 2.61 ± 1.182
0.522AsnLys: 0.522 ± 0.406
3.653AsnLeu: 3.653 ± 1.44
3.132AsnMet: 3.132 ± 1.852
2.088AsnAsn: 2.088 ± 0.969
2.088AsnPro: 2.088 ± 0.858
1.566AsnGln: 1.566 ± 1.183
2.61AsnArg: 2.61 ± 0.92
4.175AsnSer: 4.175 ± 1.261
3.132AsnThr: 3.132 ± 1.385
2.088AsnVal: 2.088 ± 1.17
0.522AsnTrp: 0.522 ± 0.406
2.61AsnTyr: 2.61 ± 1.329
0.0AsnXaa: 0.0 ± 0.0
Pro
3.132ProAla: 3.132 ± 1.102
1.044ProCys: 1.044 ± 0.871
2.088ProAsp: 2.088 ± 1.16
3.653ProGlu: 3.653 ± 1.333
1.566ProPhe: 1.566 ± 0.685
1.044ProGly: 1.044 ± 0.786
1.566ProHis: 1.566 ± 0.85
0.522ProIle: 0.522 ± 0.406
0.522ProLys: 0.522 ± 0.463
6.785ProLeu: 6.785 ± 2.012
0.0ProMet: 0.0 ± 0.0
2.61ProAsn: 2.61 ± 0.946
1.566ProPro: 1.566 ± 1.389
0.522ProGln: 0.522 ± 0.613
2.61ProArg: 2.61 ± 1.542
5.219ProSer: 5.219 ± 2.322
2.088ProThr: 2.088 ± 0.968
3.653ProVal: 3.653 ± 1.839
0.522ProTrp: 0.522 ± 0.463
1.566ProTyr: 1.566 ± 0.758
0.0ProXaa: 0.0 ± 0.0
Gln
4.697GlnAla: 4.697 ± 2.205
0.522GlnCys: 0.522 ± 0.463
1.566GlnAsp: 1.566 ± 1.512
3.653GlnGlu: 3.653 ± 1.443
1.566GlnPhe: 1.566 ± 1.036
2.61GlnGly: 2.61 ± 1.544
1.566GlnHis: 1.566 ± 0.838
3.132GlnIle: 3.132 ± 1.609
4.175GlnLys: 4.175 ± 1.587
4.697GlnLeu: 4.697 ± 1.487
1.044GlnMet: 1.044 ± 0.672
2.088GlnAsn: 2.088 ± 2.016
3.132GlnPro: 3.132 ± 1.669
3.653GlnGln: 3.653 ± 2.17
1.566GlnArg: 1.566 ± 0.502
4.697GlnSer: 4.697 ± 1.339
3.132GlnThr: 3.132 ± 1.215
3.132GlnVal: 3.132 ± 2.302
2.61GlnTrp: 2.61 ± 0.887
1.566GlnTyr: 1.566 ± 0.502
0.0GlnXaa: 0.0 ± 0.0
Arg
6.263ArgAla: 6.263 ± 1.379
0.522ArgCys: 0.522 ± 0.609
3.653ArgAsp: 3.653 ± 1.167
1.566ArgGlu: 1.566 ± 0.799
4.175ArgPhe: 4.175 ± 1.978
1.566ArgGly: 1.566 ± 0.85
1.044ArgHis: 1.044 ± 0.778
2.088ArgIle: 2.088 ± 0.858
4.697ArgLys: 4.697 ± 2.05
6.263ArgLeu: 6.263 ± 2.299
2.61ArgMet: 2.61 ± 0.958
2.088ArgAsn: 2.088 ± 0.807
2.088ArgPro: 2.088 ± 0.852
5.219ArgGln: 5.219 ± 1.926
3.132ArgArg: 3.132 ± 1.281
3.132ArgSer: 3.132 ± 1.643
2.61ArgThr: 2.61 ± 1.329
4.697ArgVal: 4.697 ± 1.386
0.522ArgTrp: 0.522 ± 0.698
3.653ArgTyr: 3.653 ± 1.063
0.0ArgXaa: 0.0 ± 0.0
Ser
7.307SerAla: 7.307 ± 2.273
0.0SerCys: 0.0 ± 0.0
4.697SerAsp: 4.697 ± 1.894
2.61SerGlu: 2.61 ± 2.116
4.697SerPhe: 4.697 ± 1.643
4.697SerGly: 4.697 ± 1.499
2.088SerHis: 2.088 ± 0.958
3.132SerIle: 3.132 ± 1.172
2.088SerLys: 2.088 ± 0.798
3.653SerLeu: 3.653 ± 2.146
3.653SerMet: 3.653 ± 1.417
6.263SerAsn: 6.263 ± 1.492
2.61SerPro: 2.61 ± 1.234
6.263SerGln: 6.263 ± 2.705
5.219SerArg: 5.219 ± 1.405
8.351SerSer: 8.351 ± 2.776
3.132SerThr: 3.132 ± 1.627
5.219SerVal: 5.219 ± 2.284
1.044SerTrp: 1.044 ± 0.817
1.566SerTyr: 1.566 ± 0.651
0.0SerXaa: 0.0 ± 0.0
Thr
4.175ThrAla: 4.175 ± 1.418
0.0ThrCys: 0.0 ± 0.0
3.653ThrAsp: 3.653 ± 1.591
4.697ThrGlu: 4.697 ± 1.226
2.61ThrPhe: 2.61 ± 1.066
3.132ThrGly: 3.132 ± 1.09
1.566ThrHis: 1.566 ± 0.502
2.088ThrIle: 2.088 ± 1.283
3.132ThrLys: 3.132 ± 1.218
7.307ThrLeu: 7.307 ± 2.842
0.522ThrMet: 0.522 ± 0.463
1.566ThrAsn: 1.566 ± 0.988
2.61ThrPro: 2.61 ± 1.208
2.088ThrGln: 2.088 ± 1.192
4.175ThrArg: 4.175 ± 1.49
4.175ThrSer: 4.175 ± 1.94
1.566ThrThr: 1.566 ± 0.988
3.132ThrVal: 3.132 ± 1.522
0.522ThrTrp: 0.522 ± 0.406
2.088ThrTyr: 2.088 ± 0.902
0.0ThrXaa: 0.0 ± 0.0
Val
6.785ValAla: 6.785 ± 2.451
0.0ValCys: 0.0 ± 0.0
5.219ValAsp: 5.219 ± 2.107
3.132ValGlu: 3.132 ± 2.039
3.653ValPhe: 3.653 ± 1.865
3.132ValGly: 3.132 ± 0.784
1.044ValHis: 1.044 ± 0.659
2.088ValIle: 2.088 ± 0.887
4.175ValLys: 4.175 ± 1.731
3.132ValLeu: 3.132 ± 1.286
2.088ValMet: 2.088 ± 1.025
4.697ValAsn: 4.697 ± 2.541
2.61ValPro: 2.61 ± 1.189
3.132ValGln: 3.132 ± 1.259
7.307ValArg: 7.307 ± 2.885
2.61ValSer: 2.61 ± 1.562
4.175ValThr: 4.175 ± 1.725
4.697ValVal: 4.697 ± 2.217
0.0ValTrp: 0.0 ± 0.0
3.132ValTyr: 3.132 ± 0.842
0.0ValXaa: 0.0 ± 0.0
Trp
1.044TrpAla: 1.044 ± 0.584
0.0TrpCys: 0.0 ± 0.0
1.044TrpAsp: 1.044 ± 0.817
0.522TrpGlu: 0.522 ± 0.504
1.044TrpPhe: 1.044 ± 0.652
0.522TrpGly: 0.522 ± 0.757
0.522TrpHis: 0.522 ± 0.406
1.044TrpIle: 1.044 ± 0.676
1.044TrpLys: 1.044 ± 0.946
1.044TrpLeu: 1.044 ± 0.652
0.522TrpMet: 0.522 ± 0.463
1.044TrpAsn: 1.044 ± 0.584
1.566TrpPro: 1.566 ± 1.217
0.522TrpGln: 0.522 ± 0.406
0.0TrpArg: 0.0 ± 0.0
0.522TrpSer: 0.522 ± 0.406
2.088TrpThr: 2.088 ± 1.03
0.0TrpVal: 0.0 ± 0.0
0.0TrpTrp: 0.0 ± 0.0
1.566TrpTyr: 1.566 ± 0.679
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.132TyrAla: 3.132 ± 1.109
0.0TyrCys: 0.0 ± 0.0
1.566TyrAsp: 1.566 ± 1.389
0.522TyrGlu: 0.522 ± 0.406
2.61TyrPhe: 2.61 ± 1.06
3.132TyrGly: 3.132 ± 0.716
0.0TyrHis: 0.0 ± 0.0
1.566TyrIle: 1.566 ± 0.791
0.0TyrLys: 0.0 ± 0.0
3.132TyrLeu: 3.132 ± 1.624
0.522TyrMet: 0.522 ± 0.406
2.088TyrAsn: 2.088 ± 0.786
1.044TyrPro: 1.044 ± 0.975
2.088TyrGln: 2.088 ± 1.623
3.653TyrArg: 3.653 ± 1.293
1.044TyrSer: 1.044 ± 0.479
1.044TyrThr: 1.044 ± 0.676
4.175TyrVal: 4.175 ± 1.147
0.0TyrTrp: 0.0 ± 0.0
1.044TyrTyr: 1.044 ± 0.851
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10 proteins (1917 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski