Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229) (Candida mogii)
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4987 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5E051|C5E051_ZYGRC Methionyl-tRNA formyltransferase mitochondrial OS=Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229) OX=559307 GN=ZYRO0G09812g PE=3 SV=1
MM1 pKa = 7.53 SAFNDD6 pKa = 3.73 FCIVCDD12 pKa = 3.61 QLIPQDD18 pKa = 4.03 SLPPRR23 pKa = 11.84 VDD25 pKa = 3.11 EE26 pKa = 5.36 LLYY29 pKa = 10.91 CSEE32 pKa = 4.02 EE33 pKa = 4.53 CMVKK37 pKa = 9.48 DD38 pKa = 4.06 TSRR41 pKa = 11.84 VPPAAAPAEE50 pKa = 4.15 LSEE53 pKa = 4.56 SASSLGDD60 pKa = 3.28 VDD62 pKa = 5.45 SLIASPLLLPMDD74 pKa = 4.08 GMVPPPASGIEE85 pKa = 4.04 DD86 pKa = 3.7 SDD88 pKa = 4.13 YY89 pKa = 10.89 MLMEE93 pKa = 5.13 LEE95 pKa = 4.61 SASQAVPRR103 pKa = 11.84 TQAVKK108 pKa = 10.23 EE109 pKa = 4.16 PCSPGAGNAVQVLDD123 pKa = 5.19 RR124 pKa = 11.84 VAEE127 pKa = 4.2 DD128 pKa = 4.41 NYY130 pKa = 11.25 KK131 pKa = 10.6 LWLSQCHH138 pKa = 4.84 WW139 pKa = 3.86
Molecular weight: 14.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.897
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.77
Rodwell 3.694
Grimsley 3.567
Solomon 3.834
Lehninger 3.783
Nozaki 3.961
DTASelect 4.164
Thurlkill 3.706
EMBOSS 3.783
Sillero 3.973
Patrickios 0.947
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.867
Protein with the highest isoelectric point:
>tr|C5DSX2|C5DSX2_ZYGRC ZYRO0C03674p OS=Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229) OX=559307 GN=ZYRO0C03674g PE=4 SV=1
MM1 pKa = 7.6 WSFARR6 pKa = 11.84 NIFQVGARR14 pKa = 11.84 RR15 pKa = 11.84 SLFTMGNWTPTTVSPLQRR33 pKa = 11.84 LLGPSPLSSGFGMGQRR49 pKa = 11.84 RR50 pKa = 11.84 WKK52 pKa = 10.77 SRR54 pKa = 11.84 GNTFQPSTLKK64 pKa = 10.3 RR65 pKa = 11.84 KK66 pKa = 9.59 RR67 pKa = 11.84 RR68 pKa = 11.84 VGFLARR74 pKa = 11.84 ARR76 pKa = 11.84 SKK78 pKa = 10.66 QGSKK82 pKa = 9.49 ILQRR86 pKa = 11.84 RR87 pKa = 11.84 KK88 pKa = 10.2 HH89 pKa = 5.52 KK90 pKa = 10.63 GRR92 pKa = 11.84 WFLTHH97 pKa = 7.07
Molecular weight: 11.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.159
ProMoST 13.642
Dawson 13.159
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.822
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.159
DTASelect 13.144
Thurlkill 13.159
EMBOSS 13.656
Sillero 13.159
Patrickios 12.544
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.271
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4987
0
4987
2475090
25
4926
496.3
55.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.839 ± 0.029
1.235 ± 0.015
5.92 ± 0.029
6.819 ± 0.038
4.327 ± 0.02
5.67 ± 0.039
2.234 ± 0.013
5.628 ± 0.022
6.675 ± 0.034
9.616 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.091 ± 0.013
5.368 ± 0.024
4.722 ± 0.031
4.488 ± 0.031
4.873 ± 0.023
8.681 ± 0.058
5.423 ± 0.031
6.028 ± 0.025
1.107 ± 0.011
3.255 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here