Eubacterium brachy ATCC 33089

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; [Eubacterium] brachy

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1479 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V1CNI1|V1CNI1_9FIRM NAD-dependent protein deacetylase OS=Eubacterium brachy ATCC 33089 OX=1321814 GN=cobB PE=3 SV=1
MM1 pKa = 7.34FFDD4 pKa = 4.48EE5 pKa = 5.68DD6 pKa = 5.2DD7 pKa = 4.1PVSWSVDD14 pKa = 2.71IGFSEE19 pKa = 4.67EE20 pKa = 4.21DD21 pKa = 3.52FGDD24 pKa = 3.74EE25 pKa = 4.12TVSPVICINPIDD37 pKa = 3.76TDD39 pKa = 3.89KK40 pKa = 11.75NSVEE44 pKa = 4.07EE45 pKa = 3.93LVGEE49 pKa = 4.2KK50 pKa = 10.52FKK52 pKa = 11.35VATVEE57 pKa = 4.03EE58 pKa = 4.6CADD61 pKa = 4.21RR62 pKa = 11.84EE63 pKa = 4.39DD64 pKa = 3.14TFYY67 pKa = 10.43IYY69 pKa = 10.4EE70 pKa = 4.22FEE72 pKa = 4.27PMVSYY77 pKa = 10.19EE78 pKa = 4.14VEE80 pKa = 3.89VLEE83 pKa = 5.25IKK85 pKa = 10.74DD86 pKa = 3.33SMAHH90 pKa = 5.04VRR92 pKa = 11.84CSGIMIVDD100 pKa = 5.34GYY102 pKa = 10.8SDD104 pKa = 4.41PYY106 pKa = 9.4VQEE109 pKa = 4.02TFEE112 pKa = 4.56IDD114 pKa = 2.63SWIPVIEE121 pKa = 5.11SVDD124 pKa = 3.25DD125 pKa = 3.54WAKK128 pKa = 11.0FEE130 pKa = 4.24NCC132 pKa = 4.35

Molecular weight: 15.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V1CKN3|V1CKN3_9FIRM tRNA-specific adenosine deaminase OS=Eubacterium brachy ATCC 33089 OX=1321814 GN=tadA PE=3 SV=1
MM1 pKa = 7.73LIGVVAFFAKK11 pKa = 10.49DD12 pKa = 3.34IIYY15 pKa = 10.45GSMVFIIVCLLSFLIGQRR33 pKa = 11.84KK34 pKa = 7.24KK35 pKa = 8.45TIKK38 pKa = 10.63FIVFYY43 pKa = 10.6IFLLLFIVFSKK54 pKa = 10.16YY55 pKa = 10.35VPVTLSNMMLMIVLCMRR72 pKa = 11.84MCMPILLYY80 pKa = 10.74GQTFLRR86 pKa = 11.84TTPVSEE92 pKa = 4.32MVTGLYY98 pKa = 10.35AIRR101 pKa = 11.84IPRR104 pKa = 11.84SFIITFAVAMRR115 pKa = 11.84FFPTAKK121 pKa = 10.29EE122 pKa = 4.21EE123 pKa = 4.03ISHH126 pKa = 6.15VRR128 pKa = 11.84DD129 pKa = 3.27AMSLRR134 pKa = 11.84GIGFSWRR141 pKa = 11.84NLRR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84PGMVFEE152 pKa = 5.06GFITPLLVRR161 pKa = 11.84ASTIAEE167 pKa = 4.12EE168 pKa = 4.33LSAASITRR176 pKa = 11.84GLDD179 pKa = 3.04NPAPRR184 pKa = 11.84TAFIKK189 pKa = 10.92LNITVKK195 pKa = 10.11DD196 pKa = 3.54TFLTVIFTLLLISVVLLRR214 pKa = 11.84TIVGRR219 pKa = 11.84WW220 pKa = 2.99

Molecular weight: 25.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1479

0

1479

455806

36

1926

308.2

34.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.238 ± 0.069

1.196 ± 0.026

5.818 ± 0.056

7.593 ± 0.082

4.363 ± 0.05

6.998 ± 0.068

1.568 ± 0.025

8.928 ± 0.065

8.925 ± 0.07

8.409 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.052 ± 0.032

5.204 ± 0.048

2.801 ± 0.037

2.444 ± 0.026

4.327 ± 0.049

6.053 ± 0.049

4.857 ± 0.047

6.83 ± 0.051

0.718 ± 0.018

3.676 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski