Amino acid dipepetide frequency for Eubacterium brachy ATCC 33089

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.386AlaAla: 4.386 ± 0.12
0.693AlaCys: 0.693 ± 0.044
3.477AlaAsp: 3.477 ± 0.093
4.397AlaGlu: 4.397 ± 0.121
2.793AlaPhe: 2.793 ± 0.084
4.594AlaGly: 4.594 ± 0.111
0.976AlaHis: 0.976 ± 0.05
5.783AlaIle: 5.783 ± 0.113
5.175AlaLys: 5.175 ± 0.13
5.627AlaLeu: 5.627 ± 0.134
2.016AlaMet: 2.016 ± 0.077
2.775AlaAsn: 2.775 ± 0.082
1.536AlaPro: 1.536 ± 0.068
1.707AlaGln: 1.707 ± 0.068
2.464AlaArg: 2.464 ± 0.074
3.186AlaSer: 3.186 ± 0.09
3.111AlaThr: 3.111 ± 0.1
5.105AlaVal: 5.105 ± 0.122
0.355AlaTrp: 0.355 ± 0.028
2.225AlaTyr: 2.225 ± 0.069
0.0AlaXaa: 0.0 ± 0.0
Cys
0.667CysAla: 0.667 ± 0.043
0.235CysCys: 0.235 ± 0.024
0.753CysAsp: 0.753 ± 0.042
0.882CysGlu: 0.882 ± 0.048
0.472CysPhe: 0.472 ± 0.034
1.211CysGly: 1.211 ± 0.061
0.298CysHis: 0.298 ± 0.024
1.064CysIle: 1.064 ± 0.054
0.952CysLys: 0.952 ± 0.05
0.823CysLeu: 0.823 ± 0.038
0.287CysMet: 0.287 ± 0.024
0.599CysAsn: 0.599 ± 0.035
0.454CysPro: 0.454 ± 0.04
0.276CysGln: 0.276 ± 0.022
0.502CysArg: 0.502 ± 0.037
0.709CysSer: 0.709 ± 0.046
0.553CysThr: 0.553 ± 0.032
0.733CysVal: 0.733 ± 0.044
0.081CysTrp: 0.081 ± 0.014
0.412CysTyr: 0.412 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.249AspAla: 3.249 ± 0.095
0.665AspCys: 0.665 ± 0.041
3.526AspAsp: 3.526 ± 0.079
5.632AspGlu: 5.632 ± 0.144
2.927AspPhe: 2.927 ± 0.074
4.399AspGly: 4.399 ± 0.12
0.706AspHis: 0.706 ± 0.039
5.423AspIle: 5.423 ± 0.115
5.149AspLys: 5.149 ± 0.126
4.23AspLeu: 4.23 ± 0.093
1.81AspMet: 1.81 ± 0.059
2.701AspAsn: 2.701 ± 0.08
1.33AspPro: 1.33 ± 0.053
0.957AspGln: 0.957 ± 0.04
2.723AspArg: 2.723 ± 0.088
3.071AspSer: 3.071 ± 0.094
2.516AspThr: 2.516 ± 0.073
4.375AspVal: 4.375 ± 0.102
0.426AspTrp: 0.426 ± 0.033
2.369AspTyr: 2.369 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
4.285GluAla: 4.285 ± 0.115
0.665GluCys: 0.665 ± 0.036
4.201GluAsp: 4.201 ± 0.114
6.169GluGlu: 6.169 ± 0.161
3.265GluPhe: 3.265 ± 0.076
3.686GluGly: 3.686 ± 0.092
1.189GluHis: 1.189 ± 0.056
7.578GluIle: 7.578 ± 0.152
9.028GluLys: 9.028 ± 0.164
6.014GluLeu: 6.014 ± 0.121
2.431GluMet: 2.431 ± 0.086
5.766GluAsn: 5.766 ± 0.123
1.494GluPro: 1.494 ± 0.07
1.786GluGln: 1.786 ± 0.066
3.409GluArg: 3.409 ± 0.085
3.556GluSer: 3.556 ± 0.094
3.249GluThr: 3.249 ± 0.088
4.842GluVal: 4.842 ± 0.133
0.386GluTrp: 0.386 ± 0.03
2.935GluTyr: 2.935 ± 0.089
0.0GluXaa: 0.0 ± 0.0
Phe
2.832PheAla: 2.832 ± 0.079
0.527PheCys: 0.527 ± 0.034
2.762PheAsp: 2.762 ± 0.077
2.903PheGlu: 2.903 ± 0.084
1.985PhePhe: 1.985 ± 0.074
3.089PheGly: 3.089 ± 0.09
0.667PheHis: 0.667 ± 0.034
3.708PheIle: 3.708 ± 0.092
3.537PheLys: 3.537 ± 0.092
3.785PheLeu: 3.785 ± 0.103
1.332PheMet: 1.332 ± 0.061
2.038PheAsn: 2.038 ± 0.069
1.336PhePro: 1.336 ± 0.05
1.108PheGln: 1.108 ± 0.05
1.7PheArg: 1.7 ± 0.062
3.041PheSer: 3.041 ± 0.085
2.422PheThr: 2.422 ± 0.077
2.938PheVal: 2.938 ± 0.085
0.362PheTrp: 0.362 ± 0.025
1.56PheTyr: 1.56 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.111GlyAla: 4.111 ± 0.114
0.965GlyCys: 0.965 ± 0.052
3.765GlyAsp: 3.765 ± 0.101
5.208GlyGlu: 5.208 ± 0.119
3.205GlyPhe: 3.205 ± 0.079
4.783GlyGly: 4.783 ± 0.181
1.093GlyHis: 1.093 ± 0.055
7.104GlyIle: 7.104 ± 0.15
6.812GlyLys: 6.812 ± 0.125
5.384GlyLeu: 5.384 ± 0.121
2.143GlyMet: 2.143 ± 0.077
3.561GlyAsn: 3.561 ± 0.087
1.191GlyPro: 1.191 ± 0.055
1.667GlyGln: 1.667 ± 0.059
3.01GlyArg: 3.01 ± 0.081
4.017GlySer: 4.017 ± 0.104
3.635GlyThr: 3.635 ± 0.118
4.871GlyVal: 4.871 ± 0.11
0.469GlyTrp: 0.469 ± 0.043
2.982GlyTyr: 2.982 ± 0.087
0.0GlyXaa: 0.0 ± 0.0
His
0.753HisAla: 0.753 ± 0.042
0.23HisCys: 0.23 ± 0.022
0.774HisAsp: 0.774 ± 0.04
0.97HisGlu: 0.97 ± 0.05
0.691HisPhe: 0.691 ± 0.033
1.183HisGly: 1.183 ± 0.053
0.309HisHis: 0.309 ± 0.026
1.441HisIle: 1.441 ± 0.062
1.207HisLys: 1.207 ± 0.049
1.371HisLeu: 1.371 ± 0.064
0.564HisMet: 0.564 ± 0.033
0.777HisAsn: 0.777 ± 0.037
0.616HisPro: 0.616 ± 0.038
0.509HisGln: 0.509 ± 0.028
0.9HisArg: 0.9 ± 0.043
0.992HisSer: 0.992 ± 0.051
0.807HisThr: 0.807 ± 0.04
0.902HisVal: 0.902 ± 0.044
0.123HisTrp: 0.123 ± 0.017
0.559HisTyr: 0.559 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
6.422IleAla: 6.422 ± 0.148
1.316IleCys: 1.316 ± 0.056
5.579IleAsp: 5.579 ± 0.118
6.481IleGlu: 6.481 ± 0.142
4.048IlePhe: 4.048 ± 0.107
6.349IleGly: 6.349 ± 0.139
1.185IleHis: 1.185 ± 0.05
6.981IleIle: 6.981 ± 0.152
6.911IleLys: 6.911 ± 0.14
8.333IleLeu: 8.333 ± 0.189
2.475IleMet: 2.475 ± 0.073
3.973IleAsn: 3.973 ± 0.114
2.896IlePro: 2.896 ± 0.087
1.994IleGln: 1.994 ± 0.067
3.624IleArg: 3.624 ± 0.101
6.544IleSer: 6.544 ± 0.132
4.386IleThr: 4.386 ± 0.095
6.268IleVal: 6.268 ± 0.119
0.57IleTrp: 0.57 ± 0.035
2.944IleTyr: 2.944 ± 0.083
0.0IleXaa: 0.0 ± 0.0
Lys
5.197LysAla: 5.197 ± 0.104
0.81LysCys: 0.81 ± 0.048
5.294LysAsp: 5.294 ± 0.136
7.279LysGlu: 7.279 ± 0.157
3.172LysPhe: 3.172 ± 0.083
5.401LysGly: 5.401 ± 0.114
1.319LysHis: 1.319 ± 0.044
8.436LysIle: 8.436 ± 0.14
8.732LysLys: 8.732 ± 0.178
6.79LysLeu: 6.79 ± 0.123
2.839LysMet: 2.839 ± 0.075
6.066LysAsn: 6.066 ± 0.137
2.141LysPro: 2.141 ± 0.069
2.174LysGln: 2.174 ± 0.064
3.901LysArg: 3.901 ± 0.1
5.25LysSer: 5.25 ± 0.11
4.66LysThr: 4.66 ± 0.106
5.636LysVal: 5.636 ± 0.127
0.636LysTrp: 0.636 ± 0.042
3.517LysTyr: 3.517 ± 0.103
0.0LysXaa: 0.0 ± 0.0
Leu
5.375LeuAla: 5.375 ± 0.115
1.055LeuCys: 1.055 ± 0.054
4.824LeuAsp: 4.824 ± 0.111
6.057LeuGlu: 6.057 ± 0.126
3.534LeuPhe: 3.534 ± 0.113
5.693LeuGly: 5.693 ± 0.123
1.36LeuHis: 1.36 ± 0.06
7.045LeuIle: 7.045 ± 0.165
7.685LeuLys: 7.685 ± 0.154
7.233LeuLeu: 7.233 ± 0.147
2.53LeuMet: 2.53 ± 0.08
4.364LeuAsn: 4.364 ± 0.098
2.933LeuPro: 2.933 ± 0.089
2.027LeuGln: 2.027 ± 0.064
3.646LeuArg: 3.646 ± 0.114
6.305LeuSer: 6.305 ± 0.115
4.037LeuThr: 4.037 ± 0.112
5.004LeuVal: 5.004 ± 0.112
0.698LeuTrp: 0.698 ± 0.04
2.681LeuTyr: 2.681 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
2.301MetAla: 2.301 ± 0.079
0.338MetCys: 0.338 ± 0.028
1.681MetAsp: 1.681 ± 0.057
2.569MetGlu: 2.569 ± 0.08
1.066MetPhe: 1.066 ± 0.051
2.168MetGly: 2.168 ± 0.077
0.369MetHis: 0.369 ± 0.027
2.44MetIle: 2.44 ± 0.083
2.986MetLys: 2.986 ± 0.077
2.494MetLeu: 2.494 ± 0.08
1.025MetMet: 1.025 ± 0.057
1.615MetAsn: 1.615 ± 0.058
0.928MetPro: 0.928 ± 0.042
0.796MetGln: 0.796 ± 0.042
1.365MetArg: 1.365 ± 0.048
1.985MetSer: 1.985 ± 0.07
1.485MetThr: 1.485 ± 0.056
1.795MetVal: 1.795 ± 0.056
0.206MetTrp: 0.206 ± 0.019
0.904MetTyr: 0.904 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
3.003AsnAla: 3.003 ± 0.084
0.667AsnCys: 0.667 ± 0.037
2.492AsnAsp: 2.492 ± 0.081
3.368AsnGlu: 3.368 ± 0.09
2.321AsnPhe: 2.321 ± 0.076
3.791AsnGly: 3.791 ± 0.12
0.836AsnHis: 0.836 ± 0.041
5.039AsnIle: 5.039 ± 0.134
4.247AsnLys: 4.247 ± 0.114
4.816AsnLeu: 4.816 ± 0.104
1.713AsnMet: 1.713 ± 0.06
2.424AsnAsn: 2.424 ± 0.075
1.996AsnPro: 1.996 ± 0.076
1.242AsnGln: 1.242 ± 0.058
2.565AsnArg: 2.565 ± 0.091
3.365AsnSer: 3.365 ± 0.095
2.475AsnThr: 2.475 ± 0.076
3.251AsnVal: 3.251 ± 0.09
0.476AsnTrp: 0.476 ± 0.032
1.948AsnTyr: 1.948 ± 0.066
0.0AsnXaa: 0.0 ± 0.0
Pro
1.654ProAla: 1.654 ± 0.072
0.338ProCys: 0.338 ± 0.033
1.713ProAsp: 1.713 ± 0.064
2.321ProGlu: 2.321 ± 0.069
1.351ProPhe: 1.351 ± 0.061
1.77ProGly: 1.77 ± 0.068
0.535ProHis: 0.535 ± 0.033
2.363ProIle: 2.363 ± 0.082
2.437ProLys: 2.437 ± 0.08
2.222ProLeu: 2.222 ± 0.073
0.84ProMet: 0.84 ± 0.046
1.323ProAsn: 1.323 ± 0.057
0.649ProPro: 0.649 ± 0.036
0.871ProGln: 0.871 ± 0.046
0.939ProArg: 0.939 ± 0.043
1.534ProSer: 1.534 ± 0.06
1.452ProThr: 1.452 ± 0.061
2.372ProVal: 2.372 ± 0.083
0.246ProTrp: 0.246 ± 0.02
1.082ProTyr: 1.082 ± 0.045
0.0ProXaa: 0.0 ± 0.0
Gln
1.444GlnAla: 1.444 ± 0.066
0.296GlnCys: 0.296 ± 0.026
1.178GlnAsp: 1.178 ± 0.048
1.692GlnGlu: 1.692 ± 0.055
0.981GlnPhe: 0.981 ± 0.049
1.731GlnGly: 1.731 ± 0.063
0.399GlnHis: 0.399 ± 0.031
2.26GlnIle: 2.26 ± 0.069
2.17GlnLys: 2.17 ± 0.071
2.275GlnLeu: 2.275 ± 0.068
0.818GlnMet: 0.818 ± 0.04
1.404GlnAsn: 1.404 ± 0.056
0.603GlnPro: 0.603 ± 0.041
0.685GlnGln: 0.685 ± 0.046
1.268GlnArg: 1.268 ± 0.05
1.435GlnSer: 1.435 ± 0.064
0.992GlnThr: 0.992 ± 0.045
1.626GlnVal: 1.626 ± 0.062
0.252GlnTrp: 0.252 ± 0.024
0.937GlnTyr: 0.937 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
2.413ArgAla: 2.413 ± 0.075
0.467ArgCys: 0.467 ± 0.029
2.688ArgAsp: 2.688 ± 0.075
4.217ArgGlu: 4.217 ± 0.121
1.722ArgPhe: 1.722 ± 0.063
2.984ArgGly: 2.984 ± 0.097
0.685ArgHis: 0.685 ± 0.037
3.699ArgIle: 3.699 ± 0.093
4.168ArgLys: 4.168 ± 0.098
3.512ArgLeu: 3.512 ± 0.084
1.24ArgMet: 1.24 ± 0.052
2.648ArgAsn: 2.648 ± 0.079
0.974ArgPro: 0.974 ± 0.049
1.325ArgGln: 1.325 ± 0.06
2.137ArgArg: 2.137 ± 0.073
1.964ArgSer: 1.964 ± 0.069
1.904ArgThr: 1.904 ± 0.062
2.591ArgVal: 2.591 ± 0.074
0.338ArgTrp: 0.338 ± 0.03
1.599ArgTyr: 1.599 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
3.662SerAla: 3.662 ± 0.1
0.737SerCys: 0.737 ± 0.039
3.565SerAsp: 3.565 ± 0.097
4.302SerGlu: 4.302 ± 0.111
2.705SerPhe: 2.705 ± 0.083
4.998SerGly: 4.998 ± 0.114
1.147SerHis: 1.147 ± 0.053
5.366SerIle: 5.366 ± 0.123
5.162SerLys: 5.162 ± 0.117
5.088SerLeu: 5.088 ± 0.115
1.773SerMet: 1.773 ± 0.065
2.694SerAsn: 2.694 ± 0.087
1.678SerPro: 1.678 ± 0.065
1.768SerGln: 1.768 ± 0.06
2.554SerArg: 2.554 ± 0.094
3.594SerSer: 3.594 ± 0.109
2.788SerThr: 2.788 ± 0.072
4.199SerVal: 4.199 ± 0.087
0.426SerTrp: 0.426 ± 0.03
2.326SerTyr: 2.326 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
3.587ThrAla: 3.587 ± 0.096
0.478ThrCys: 0.478 ± 0.037
2.791ThrAsp: 2.791 ± 0.078
3.065ThrGlu: 3.065 ± 0.075
2.201ThrPhe: 2.201 ± 0.08
4.179ThrGly: 4.179 ± 0.105
0.897ThrHis: 0.897 ± 0.046
3.896ThrIle: 3.896 ± 0.104
3.523ThrLys: 3.523 ± 0.104
4.522ThrLeu: 4.522 ± 0.109
1.297ThrMet: 1.297 ± 0.055
1.847ThrAsn: 1.847 ± 0.062
1.876ThrPro: 1.876 ± 0.08
1.053ThrGln: 1.053 ± 0.054
1.707ThrArg: 1.707 ± 0.058
2.894ThrSer: 2.894 ± 0.08
2.497ThrThr: 2.497 ± 0.085
4.177ThrVal: 4.177 ± 0.108
0.391ThrTrp: 0.391 ± 0.031
1.696ThrTyr: 1.696 ± 0.068
0.0ThrXaa: 0.0 ± 0.0
Val
4.594ValAla: 4.594 ± 0.1
0.834ValCys: 0.834 ± 0.046
4.234ValAsp: 4.234 ± 0.095
5.189ValGlu: 5.189 ± 0.134
3.078ValPhe: 3.078 ± 0.083
4.713ValGly: 4.713 ± 0.101
0.928ValHis: 0.928 ± 0.049
5.983ValIle: 5.983 ± 0.125
5.746ValLys: 5.746 ± 0.114
5.899ValLeu: 5.899 ± 0.124
2.119ValMet: 2.119 ± 0.071
3.311ValAsn: 3.311 ± 0.089
2.091ValPro: 2.091 ± 0.078
1.391ValGln: 1.391 ± 0.056
2.611ValArg: 2.611 ± 0.076
4.379ValSer: 4.379 ± 0.103
3.534ValThr: 3.534 ± 0.1
4.912ValVal: 4.912 ± 0.123
0.41ValTrp: 0.41 ± 0.033
2.347ValTyr: 2.347 ± 0.073
0.0ValXaa: 0.0 ± 0.0
Trp
0.412TrpAla: 0.412 ± 0.032
0.118TrpCys: 0.118 ± 0.015
0.472TrpAsp: 0.472 ± 0.029
0.437TrpGlu: 0.437 ± 0.031
0.298TrpPhe: 0.298 ± 0.028
0.472TrpGly: 0.472 ± 0.034
0.132TrpHis: 0.132 ± 0.017
0.706TrpIle: 0.706 ± 0.047
0.695TrpLys: 0.695 ± 0.048
0.678TrpLeu: 0.678 ± 0.04
0.202TrpMet: 0.202 ± 0.021
0.421TrpAsn: 0.421 ± 0.029
0.145TrpPro: 0.145 ± 0.02
0.25TrpGln: 0.25 ± 0.028
0.358TrpArg: 0.358 ± 0.031
0.397TrpSer: 0.397 ± 0.031
0.276TrpThr: 0.276 ± 0.027
0.362TrpVal: 0.362 ± 0.029
0.05TrpTrp: 0.05 ± 0.009
0.298TrpTyr: 0.298 ± 0.027
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.034TyrAla: 2.034 ± 0.064
0.529TyrCys: 0.529 ± 0.042
2.413TyrAsp: 2.413 ± 0.071
2.797TyrGlu: 2.797 ± 0.077
1.814TyrPhe: 1.814 ± 0.065
2.784TyrGly: 2.784 ± 0.084
0.647TyrHis: 0.647 ± 0.038
2.964TyrIle: 2.964 ± 0.076
2.927TyrLys: 2.927 ± 0.079
2.993TyrLeu: 2.993 ± 0.089
1.062TyrMet: 1.062 ± 0.044
1.731TyrAsn: 1.731 ± 0.059
1.145TyrPro: 1.145 ± 0.055
0.858TyrGln: 0.858 ± 0.05
1.893TyrArg: 1.893 ± 0.06
2.317TyrSer: 2.317 ± 0.073
1.792TyrThr: 1.792 ± 0.074
2.343TyrVal: 2.343 ± 0.076
0.279TyrTrp: 0.279 ± 0.023
1.435TyrTyr: 1.435 ± 0.068
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1479 proteins (455806 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski