bacterium G20

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A202DRG4|A0A202DRG4_9BACT Superoxide dismutase OS=bacterium G20 OX=1932699 GN=BVY00_02510 PE=3 SV=1
MM1 pKa = 7.77ASTFSILSSTYY12 pKa = 9.63TNTASGTTINGDD24 pKa = 3.22LGYY27 pKa = 7.57TTGPAVAPTVNGITHH42 pKa = 7.03IADD45 pKa = 3.26GTYY48 pKa = 9.91NQAGIDD54 pKa = 3.52QGTALSALNSQACTFTFPAGAVDD77 pKa = 4.5LATDD81 pKa = 4.0TSHH84 pKa = 6.57GVIGIYY90 pKa = 9.74TPGVYY95 pKa = 8.72CTTASSAASIGTAGITLSGSGTYY118 pKa = 9.8IFRR121 pKa = 11.84VNGALTTVANSAVRR135 pKa = 11.84LASSASACDD144 pKa = 3.7IFWTPTAATTLGANSTFIGTDD165 pKa = 2.6IDD167 pKa = 3.83ASGVTIGDD175 pKa = 4.32TITT178 pKa = 3.3

Molecular weight: 17.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A202DTP1|A0A202DTP1_9BACT Uncharacterized protein OS=bacterium G20 OX=1932699 GN=BVY00_00510 PE=4 SV=1
MM1 pKa = 7.22GQGASQPQPASEE13 pKa = 3.95TGRR16 pKa = 11.84RR17 pKa = 11.84HH18 pKa = 5.81RR19 pKa = 11.84PDD21 pKa = 3.15YY22 pKa = 11.05AILVLTALLLVIGLIVVYY40 pKa = 10.18SISPGLSASQHH51 pKa = 4.64VSQSYY56 pKa = 10.32FITKK60 pKa = 9.82QLLDD64 pKa = 3.7VALGAAAFAIAAVLPLRR81 pKa = 11.84RR82 pKa = 11.84WSAFSRR88 pKa = 11.84SLIVATVVGSVIVMITPINAIYY110 pKa = 8.51PAHH113 pKa = 6.01RR114 pKa = 11.84WIRR117 pKa = 11.84IGGFSFQVAEE127 pKa = 4.79LIKK130 pKa = 10.17LTLIVGLANFLSGQWRR146 pKa = 11.84KK147 pKa = 10.03GKK149 pKa = 10.02LADD152 pKa = 5.03FKK154 pKa = 11.23ATLKK158 pKa = 10.66PLIIVLLGVGIVVAKK173 pKa = 10.02LQSDD177 pKa = 4.27LGSAGVMIVIMAAMAFTVGIPLKK200 pKa = 10.47KK201 pKa = 10.18VALICALVTLVFALAIASSGYY222 pKa = 9.48RR223 pKa = 11.84RR224 pKa = 11.84QRR226 pKa = 11.84LATFLHH232 pKa = 6.78PSSDD236 pKa = 3.88CRR238 pKa = 11.84SSGYY242 pKa = 9.6HH243 pKa = 6.09ACQALIAVGSGGVFGEE259 pKa = 4.23GLGFGAASYY268 pKa = 10.59GYY270 pKa = 10.51LPEE273 pKa = 4.74SSNDD277 pKa = 3.58SIFAIMAQKK286 pKa = 10.44FGFIGTTLIVTIYY299 pKa = 10.56GFFITRR305 pKa = 11.84IKK307 pKa = 10.69KK308 pKa = 10.37LIEE311 pKa = 3.85RR312 pKa = 11.84TNDD315 pKa = 2.95QFSRR319 pKa = 11.84LILVGVLAWLSTQTIVNIGAMLGLLPLKK347 pKa = 10.62GITLPLISQGGTSLIFLTAALGIVFQISRR376 pKa = 11.84YY377 pKa = 6.72TSYY380 pKa = 11.53NVTEE384 pKa = 4.51PKK386 pKa = 9.88TGSEE390 pKa = 3.94QGSNTNSSSNRR401 pKa = 11.84RR402 pKa = 11.84RR403 pKa = 11.84LRR405 pKa = 11.84RR406 pKa = 11.84PYY408 pKa = 10.41NPPTISRR415 pKa = 11.84PRR417 pKa = 11.84TT418 pKa = 3.33

Molecular weight: 44.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

493

0

493

119114

38

1880

241.6

26.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.946 ± 0.119

0.541 ± 0.032

5.158 ± 0.104

5.974 ± 0.195

3.553 ± 0.082

7.224 ± 0.148

2.082 ± 0.058

6.415 ± 0.106

6.772 ± 0.149

9.705 ± 0.13

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.938 ± 0.059

4.096 ± 0.132

4.531 ± 0.11

4.071 ± 0.078

5.286 ± 0.114

6.531 ± 0.147

5.937 ± 0.21

7.013 ± 0.08

1.082 ± 0.048

3.146 ± 0.076
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski