Amino acid dipepetide frequency for bacterium G20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.613AlaAla: 9.613 ± 0.297
0.411AlaCys: 0.411 ± 0.055
4.441AlaAsp: 4.441 ± 0.22
5.524AlaGlu: 5.524 ± 0.27
2.762AlaPhe: 2.762 ± 0.174
7.128AlaGly: 7.128 ± 0.329
1.914AlaHis: 1.914 ± 0.134
5.264AlaIle: 5.264 ± 0.241
6.347AlaLys: 6.347 ± 0.251
8.236AlaLeu: 8.236 ± 0.284
1.797AlaMet: 1.797 ± 0.143
3.442AlaAsn: 3.442 ± 0.209
3.459AlaPro: 3.459 ± 0.217
3.954AlaGln: 3.954 ± 0.194
4.936AlaArg: 4.936 ± 0.221
5.558AlaSer: 5.558 ± 0.243
5.146AlaThr: 5.146 ± 0.243
6.087AlaVal: 6.087 ± 0.245
1.058AlaTrp: 1.058 ± 0.102
2.384AlaTyr: 2.384 ± 0.155
0.0AlaXaa: 0.0 ± 0.0
Cys
0.428CysAla: 0.428 ± 0.069
0.008CysCys: 0.008 ± 0.007
0.319CysAsp: 0.319 ± 0.048
0.277CysGlu: 0.277 ± 0.044
0.235CysPhe: 0.235 ± 0.045
0.445CysGly: 0.445 ± 0.064
0.143CysHis: 0.143 ± 0.036
0.311CysIle: 0.311 ± 0.048
0.285CysLys: 0.285 ± 0.053
0.42CysLeu: 0.42 ± 0.067
0.118CysMet: 0.118 ± 0.031
0.269CysAsn: 0.269 ± 0.043
0.336CysPro: 0.336 ± 0.061
0.252CysGln: 0.252 ± 0.042
0.21CysArg: 0.21 ± 0.043
0.361CysSer: 0.361 ± 0.056
0.369CysThr: 0.369 ± 0.059
0.361CysVal: 0.361 ± 0.055
0.042CysTrp: 0.042 ± 0.02
0.227CysTyr: 0.227 ± 0.043
0.0CysXaa: 0.0 ± 0.0
Asp
3.618AspAla: 3.618 ± 0.182
0.311AspCys: 0.311 ± 0.049
2.603AspAsp: 2.603 ± 0.135
3.652AspGlu: 3.652 ± 0.222
2.225AspPhe: 2.225 ± 0.138
3.803AspGly: 3.803 ± 0.187
1.301AspHis: 1.301 ± 0.115
3.291AspIle: 3.291 ± 0.195
3.879AspLys: 3.879 ± 0.228
5.004AspLeu: 5.004 ± 0.273
1.016AspMet: 1.016 ± 0.092
1.839AspAsn: 1.839 ± 0.13
2.544AspPro: 2.544 ± 0.125
2.191AspGln: 2.191 ± 0.151
2.418AspArg: 2.418 ± 0.152
2.98AspSer: 2.98 ± 0.173
2.678AspThr: 2.678 ± 0.152
3.308AspVal: 3.308 ± 0.171
0.747AspTrp: 0.747 ± 0.088
2.174AspTyr: 2.174 ± 0.176
0.0AspXaa: 0.0 ± 0.0
Glu
6.019GluAla: 6.019 ± 0.308
0.227GluCys: 0.227 ± 0.048
3.064GluAsp: 3.064 ± 0.215
4.047GluGlu: 4.047 ± 0.266
1.889GluPhe: 1.889 ± 0.122
3.795GluGly: 3.795 ± 0.217
1.284GluHis: 1.284 ± 0.111
4.089GluIle: 4.089 ± 0.226
4.391GluLys: 4.391 ± 0.283
6.649GluLeu: 6.649 ± 0.291
1.243GluMet: 1.243 ± 0.1
2.141GluAsn: 2.141 ± 0.15
2.326GluPro: 2.326 ± 0.164
2.888GluGln: 2.888 ± 0.163
3.383GluArg: 3.383 ± 0.198
2.552GluSer: 2.552 ± 0.159
2.888GluThr: 2.888 ± 0.184
4.609GluVal: 4.609 ± 0.229
0.537GluTrp: 0.537 ± 0.073
1.721GluTyr: 1.721 ± 0.134
0.0GluXaa: 0.0 ± 0.0
Phe
2.737PheAla: 2.737 ± 0.175
0.218PheCys: 0.218 ± 0.042
2.048PheAsp: 2.048 ± 0.139
2.166PheGlu: 2.166 ± 0.147
1.536PhePhe: 1.536 ± 0.125
3.131PheGly: 3.131 ± 0.175
0.697PheHis: 0.697 ± 0.078
2.544PheIle: 2.544 ± 0.182
2.426PheLys: 2.426 ± 0.146
3.106PheLeu: 3.106 ± 0.174
0.571PheMet: 0.571 ± 0.077
1.612PheAsn: 1.612 ± 0.114
1.31PhePro: 1.31 ± 0.121
0.957PheGln: 0.957 ± 0.118
1.687PheArg: 1.687 ± 0.127
2.51PheSer: 2.51 ± 0.16
1.83PheThr: 1.83 ± 0.143
2.586PheVal: 2.586 ± 0.137
0.546PheTrp: 0.546 ± 0.068
1.301PheTyr: 1.301 ± 0.107
0.0PheXaa: 0.0 ± 0.0
Gly
5.524GlyAla: 5.524 ± 0.278
0.437GlyCys: 0.437 ± 0.064
3.022GlyAsp: 3.022 ± 0.157
4.097GlyGlu: 4.097 ± 0.242
2.964GlyPhe: 2.964 ± 0.163
5.566GlyGly: 5.566 ± 0.346
1.604GlyHis: 1.604 ± 0.114
4.752GlyIle: 4.752 ± 0.189
4.508GlyLys: 4.508 ± 0.206
7.161GlyLeu: 7.161 ± 0.264
1.469GlyMet: 1.469 ± 0.104
2.913GlyAsn: 2.913 ± 0.26
2.401GlyPro: 2.401 ± 0.148
3.299GlyGln: 3.299 ± 0.17
4.139GlyArg: 4.139 ± 0.208
5.256GlySer: 5.256 ± 0.359
4.567GlyThr: 4.567 ± 0.474
5.449GlyVal: 5.449 ± 0.212
0.89GlyTrp: 0.89 ± 0.088
2.225GlyTyr: 2.225 ± 0.126
0.0GlyXaa: 0.0 ± 0.0
His
1.268HisAla: 1.268 ± 0.097
0.101HisCys: 0.101 ± 0.028
1.091HisAsp: 1.091 ± 0.095
1.352HisGlu: 1.352 ± 0.113
0.949HisPhe: 0.949 ± 0.106
1.377HisGly: 1.377 ± 0.13
0.512HisHis: 0.512 ± 0.077
1.377HisIle: 1.377 ± 0.104
1.318HisLys: 1.318 ± 0.095
2.065HisLeu: 2.065 ± 0.127
0.353HisMet: 0.353 ± 0.053
0.831HisAsn: 0.831 ± 0.098
1.234HisPro: 1.234 ± 0.106
0.974HisGln: 0.974 ± 0.106
1.175HisArg: 1.175 ± 0.087
1.318HisSer: 1.318 ± 0.103
1.041HisThr: 1.041 ± 0.094
1.343HisVal: 1.343 ± 0.108
0.294HisTrp: 0.294 ± 0.051
0.848HisTyr: 0.848 ± 0.087
0.0HisXaa: 0.0 ± 0.0
Ile
5.633IleAla: 5.633 ± 0.227
0.395IleCys: 0.395 ± 0.062
4.08IleAsp: 4.08 ± 0.201
4.147IleGlu: 4.147 ± 0.238
2.309IlePhe: 2.309 ± 0.17
4.643IleGly: 4.643 ± 0.251
1.326IleHis: 1.326 ± 0.094
4.315IleIle: 4.315 ± 0.256
4.256IleLys: 4.256 ± 0.184
6.011IleLeu: 6.011 ± 0.252
1.083IleMet: 1.083 ± 0.105
3.039IleAsn: 3.039 ± 0.161
2.51IlePro: 2.51 ± 0.142
1.679IleGln: 1.679 ± 0.132
3.014IleArg: 3.014 ± 0.176
4.433IleSer: 4.433 ± 0.216
3.879IleThr: 3.879 ± 0.176
4.945IleVal: 4.945 ± 0.244
0.512IleTrp: 0.512 ± 0.062
1.939IleTyr: 1.939 ± 0.132
0.0IleXaa: 0.0 ± 0.0
Lys
5.642LysAla: 5.642 ± 0.25
0.227LysCys: 0.227 ± 0.049
4.038LysAsp: 4.038 ± 0.218
3.837LysGlu: 3.837 ± 0.232
1.864LysPhe: 1.864 ± 0.127
3.728LysGly: 3.728 ± 0.225
1.142LysHis: 1.142 ± 0.097
4.592LysIle: 4.592 ± 0.212
5.91LysLys: 5.91 ± 0.335
6.716LysLeu: 6.716 ± 0.256
1.52LysMet: 1.52 ± 0.125
3.249LysAsn: 3.249 ± 0.154
3.241LysPro: 3.241 ± 0.18
3.056LysGln: 3.056 ± 0.158
3.593LysArg: 3.593 ± 0.197
3.795LysSer: 3.795 ± 0.22
4.424LysThr: 4.424 ± 0.201
4.433LysVal: 4.433 ± 0.227
0.613LysTrp: 0.613 ± 0.086
2.099LysTyr: 2.099 ± 0.121
0.0LysXaa: 0.0 ± 0.0
Leu
9.881LeuAla: 9.881 ± 0.354
0.537LeuCys: 0.537 ± 0.071
4.936LeuAsp: 4.936 ± 0.183
5.717LeuGlu: 5.717 ± 0.273
2.964LeuPhe: 2.964 ± 0.224
7.144LeuGly: 7.144 ± 0.258
1.965LeuHis: 1.965 ± 0.133
6.221LeuIle: 6.221 ± 0.246
6.464LeuLys: 6.464 ± 0.263
9.025LeuLeu: 9.025 ± 0.371
1.931LeuMet: 1.931 ± 0.126
3.937LeuAsn: 3.937 ± 0.209
4.575LeuPro: 4.575 ± 0.186
4.063LeuGln: 4.063 ± 0.232
5.029LeuArg: 5.029 ± 0.219
6.767LeuSer: 6.767 ± 0.236
5.558LeuThr: 5.558 ± 0.228
6.649LeuVal: 6.649 ± 0.265
0.949LeuTrp: 0.949 ± 0.088
2.737LeuTyr: 2.737 ± 0.157
0.0LeuXaa: 0.0 ± 0.0
Met
2.023MetAla: 2.023 ± 0.141
0.067MetCys: 0.067 ± 0.024
1.007MetAsp: 1.007 ± 0.105
1.033MetGlu: 1.033 ± 0.099
0.562MetPhe: 0.562 ± 0.074
1.142MetGly: 1.142 ± 0.101
0.42MetHis: 0.42 ± 0.06
1.142MetIle: 1.142 ± 0.107
1.184MetLys: 1.184 ± 0.096
1.939MetLeu: 1.939 ± 0.113
0.369MetMet: 0.369 ± 0.065
0.688MetAsn: 0.688 ± 0.081
1.276MetPro: 1.276 ± 0.104
0.873MetGln: 0.873 ± 0.084
1.284MetArg: 1.284 ± 0.094
1.402MetSer: 1.402 ± 0.119
1.041MetThr: 1.041 ± 0.098
1.201MetVal: 1.201 ± 0.11
0.185MetTrp: 0.185 ± 0.04
0.537MetTyr: 0.537 ± 0.065
0.0MetXaa: 0.0 ± 0.0
Asn
3.115AsnAla: 3.115 ± 0.292
0.336AsnCys: 0.336 ± 0.057
1.855AsnAsp: 1.855 ± 0.136
2.208AsnGlu: 2.208 ± 0.154
1.536AsnPhe: 1.536 ± 0.111
2.922AsnGly: 2.922 ± 0.245
0.722AsnHis: 0.722 ± 0.078
2.754AsnIle: 2.754 ± 0.17
2.787AsnLys: 2.787 ± 0.152
4.24AsnLeu: 4.24 ± 0.228
0.873AsnMet: 0.873 ± 0.087
1.872AsnAsn: 1.872 ± 0.177
2.057AsnPro: 2.057 ± 0.144
1.746AsnGln: 1.746 ± 0.116
2.124AsnArg: 2.124 ± 0.129
2.838AsnSer: 2.838 ± 0.272
2.317AsnThr: 2.317 ± 0.259
2.77AsnVal: 2.77 ± 0.17
0.428AsnTrp: 0.428 ± 0.06
1.461AsnTyr: 1.461 ± 0.124
0.0AsnXaa: 0.0 ± 0.0
Pro
4.626ProAla: 4.626 ± 0.293
0.201ProCys: 0.201 ± 0.044
2.527ProAsp: 2.527 ± 0.145
3.35ProGlu: 3.35 ± 0.2
1.729ProPhe: 1.729 ± 0.132
3.056ProGly: 3.056 ± 0.156
1.066ProHis: 1.066 ± 0.101
2.401ProIle: 2.401 ± 0.152
3.148ProLys: 3.148 ± 0.183
3.467ProLeu: 3.467 ± 0.185
0.739ProMet: 0.739 ± 0.073
1.897ProAsn: 1.897 ± 0.128
2.468ProPro: 2.468 ± 0.25
1.595ProGln: 1.595 ± 0.108
1.847ProArg: 1.847 ± 0.142
3.215ProSer: 3.215 ± 0.179
2.997ProThr: 2.997 ± 0.191
3.249ProVal: 3.249 ± 0.223
0.378ProTrp: 0.378 ± 0.058
1.352ProTyr: 1.352 ± 0.124
0.0ProXaa: 0.0 ± 0.0
Gln
4.072GlnAla: 4.072 ± 0.19
0.134GlnCys: 0.134 ± 0.034
1.746GlnAsp: 1.746 ± 0.12
2.023GlnGlu: 2.023 ± 0.123
1.427GlnPhe: 1.427 ± 0.107
2.275GlnGly: 2.275 ± 0.157
0.739GlnHis: 0.739 ± 0.079
3.073GlnIle: 3.073 ± 0.173
3.123GlnLys: 3.123 ± 0.155
4.534GlnLeu: 4.534 ± 0.22
0.873GlnMet: 0.873 ± 0.087
1.83GlnAsn: 1.83 ± 0.166
2.116GlnPro: 2.116 ± 0.145
1.562GlnGln: 1.562 ± 0.123
2.326GlnArg: 2.326 ± 0.155
2.082GlnSer: 2.082 ± 0.15
2.653GlnThr: 2.653 ± 0.201
2.762GlnVal: 2.762 ± 0.142
0.395GlnTrp: 0.395 ± 0.063
0.965GlnTyr: 0.965 ± 0.105
0.0GlnXaa: 0.0 ± 0.0
Arg
4.114ArgAla: 4.114 ± 0.217
0.193ArgCys: 0.193 ± 0.039
2.51ArgAsp: 2.51 ± 0.158
3.375ArgGlu: 3.375 ± 0.196
1.889ArgPhe: 1.889 ± 0.128
3.593ArgGly: 3.593 ± 0.183
1.368ArgHis: 1.368 ± 0.138
3.014ArgIle: 3.014 ± 0.173
3.157ArgLys: 3.157 ± 0.191
5.759ArgLeu: 5.759 ± 0.237
0.982ArgMet: 0.982 ± 0.093
1.788ArgAsn: 1.788 ± 0.111
2.435ArgPro: 2.435 ± 0.119
2.729ArgGln: 2.729 ± 0.168
3.862ArgArg: 3.862 ± 0.196
3.291ArgSer: 3.291 ± 0.154
2.577ArgThr: 2.577 ± 0.145
3.644ArgVal: 3.644 ± 0.179
0.621ArgTrp: 0.621 ± 0.062
1.956ArgTyr: 1.956 ± 0.129
0.0ArgXaa: 0.0 ± 0.0
Ser
5.339SerAla: 5.339 ± 0.295
0.395SerCys: 0.395 ± 0.066
3.09SerAsp: 3.09 ± 0.157
3.333SerGlu: 3.333 ± 0.17
2.418SerPhe: 2.418 ± 0.157
5.684SerGly: 5.684 ± 0.283
1.377SerHis: 1.377 ± 0.122
3.795SerIle: 3.795 ± 0.215
3.904SerLys: 3.904 ± 0.177
6.364SerLeu: 6.364 ± 0.238
1.293SerMet: 1.293 ± 0.102
2.577SerAsn: 2.577 ± 0.187
3.014SerPro: 3.014 ± 0.182
2.888SerGln: 2.888 ± 0.173
3.283SerArg: 3.283 ± 0.166
5.256SerSer: 5.256 ± 0.353
4.265SerThr: 4.265 ± 0.427
4.382SerVal: 4.382 ± 0.228
0.588SerTrp: 0.588 ± 0.082
2.065SerTyr: 2.065 ± 0.124
0.0SerXaa: 0.0 ± 0.0
Thr
5.986ThrAla: 5.986 ± 0.372
0.294ThrCys: 0.294 ± 0.053
3.325ThrAsp: 3.325 ± 0.187
2.947ThrGlu: 2.947 ± 0.168
1.805ThrPhe: 1.805 ± 0.131
4.651ThrGly: 4.651 ± 0.283
1.058ThrHis: 1.058 ± 0.093
3.988ThrIle: 3.988 ± 0.206
3.602ThrLys: 3.602 ± 0.175
5.507ThrLeu: 5.507 ± 0.24
0.974ThrMet: 0.974 ± 0.092
2.603ThrAsn: 2.603 ± 0.35
3.173ThrPro: 3.173 ± 0.211
1.948ThrGln: 1.948 ± 0.131
2.317ThrArg: 2.317 ± 0.154
4.122ThrSer: 4.122 ± 0.265
4.651ThrThr: 4.651 ± 0.422
3.895ThrVal: 3.895 ± 0.223
0.663ThrTrp: 0.663 ± 0.08
1.864ThrTyr: 1.864 ± 0.139
0.0ThrXaa: 0.0 ± 0.0
Val
6.716ValAla: 6.716 ± 0.259
0.554ValCys: 0.554 ± 0.095
3.702ValAsp: 3.702 ± 0.177
4.181ValGlu: 4.181 ± 0.265
2.376ValPhe: 2.376 ± 0.147
5.155ValGly: 5.155 ± 0.218
1.217ValHis: 1.217 ± 0.105
4.936ValIle: 4.936 ± 0.248
4.206ValLys: 4.206 ± 0.222
6.607ValLeu: 6.607 ± 0.187
1.326ValMet: 1.326 ± 0.103
2.871ValAsn: 2.871 ± 0.186
2.989ValPro: 2.989 ± 0.186
2.057ValGln: 2.057 ± 0.131
3.761ValArg: 3.761 ± 0.184
4.819ValSer: 4.819 ± 0.199
4.131ValThr: 4.131 ± 0.227
5.717ValVal: 5.717 ± 0.203
0.672ValTrp: 0.672 ± 0.085
2.132ValTyr: 2.132 ± 0.148
0.0ValXaa: 0.0 ± 0.0
Trp
0.688TrpAla: 0.688 ± 0.078
0.109TrpCys: 0.109 ± 0.029
0.604TrpAsp: 0.604 ± 0.066
0.462TrpGlu: 0.462 ± 0.073
0.546TrpPhe: 0.546 ± 0.091
0.63TrpGly: 0.63 ± 0.07
0.285TrpHis: 0.285 ± 0.051
0.504TrpIle: 0.504 ± 0.054
0.462TrpLys: 0.462 ± 0.062
1.41TrpLeu: 1.41 ± 0.119
0.218TrpMet: 0.218 ± 0.044
0.285TrpAsn: 0.285 ± 0.05
0.437TrpPro: 0.437 ± 0.066
0.688TrpGln: 0.688 ± 0.09
0.73TrpArg: 0.73 ± 0.073
0.806TrpSer: 0.806 ± 0.113
0.705TrpThr: 0.705 ± 0.078
0.604TrpVal: 0.604 ± 0.074
0.277TrpTrp: 0.277 ± 0.054
0.369TrpTyr: 0.369 ± 0.057
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.418TyrAla: 2.418 ± 0.13
0.26TyrCys: 0.26 ± 0.043
1.57TyrAsp: 1.57 ± 0.115
2.015TyrGlu: 2.015 ± 0.138
1.545TyrPhe: 1.545 ± 0.114
2.376TyrGly: 2.376 ± 0.131
0.68TyrHis: 0.68 ± 0.081
1.788TyrIle: 1.788 ± 0.121
2.359TyrLys: 2.359 ± 0.16
2.829TyrLeu: 2.829 ± 0.163
0.621TyrMet: 0.621 ± 0.067
1.276TyrAsn: 1.276 ± 0.113
1.41TyrPro: 1.41 ± 0.112
1.31TyrGln: 1.31 ± 0.11
1.738TyrArg: 1.738 ± 0.144
1.948TyrSer: 1.948 ± 0.124
1.654TyrThr: 1.654 ± 0.153
2.132TyrVal: 2.132 ± 0.15
0.428TyrTrp: 0.428 ± 0.068
1.1TyrTyr: 1.1 ± 0.102
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 493 proteins (119114 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski