Amino acid dipepetide frequency for Roseburia phage Jekyll

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.415AlaAla: 4.415 ± 0.682
0.641AlaCys: 0.641 ± 0.247
5.91AlaAsp: 5.91 ± 0.653
6.836AlaGlu: 6.836 ± 0.651
1.994AlaPhe: 1.994 ± 0.446
3.774AlaGly: 3.774 ± 0.57
1.282AlaHis: 1.282 ± 0.36
4.629AlaIle: 4.629 ± 0.536
5.839AlaLys: 5.839 ± 0.763
4.557AlaLeu: 4.557 ± 0.595
1.709AlaMet: 1.709 ± 0.291
3.56AlaAsn: 3.56 ± 0.493
0.926AlaPro: 0.926 ± 0.315
2.92AlaGln: 2.92 ± 0.577
2.136AlaArg: 2.136 ± 0.494
3.845AlaSer: 3.845 ± 0.748
3.062AlaThr: 3.062 ± 0.631
3.276AlaVal: 3.276 ± 0.396
0.997AlaTrp: 0.997 ± 0.267
2.564AlaTyr: 2.564 ± 0.409
0.0AlaXaa: 0.0 ± 0.0
Cys
0.427CysAla: 0.427 ± 0.173
0.142CysCys: 0.142 ± 0.093
1.638CysAsp: 1.638 ± 0.393
1.353CysGlu: 1.353 ± 0.306
0.427CysPhe: 0.427 ± 0.171
1.424CysGly: 1.424 ± 0.438
0.356CysHis: 0.356 ± 0.175
1.068CysIle: 1.068 ± 0.296
1.139CysLys: 1.139 ± 0.25
0.926CysLeu: 0.926 ± 0.242
0.57CysMet: 0.57 ± 0.214
0.926CysAsn: 0.926 ± 0.293
1.211CysPro: 1.211 ± 0.373
0.356CysGln: 0.356 ± 0.177
0.427CysArg: 0.427 ± 0.15
0.783CysSer: 0.783 ± 0.256
1.068CysThr: 1.068 ± 0.347
1.139CysVal: 1.139 ± 0.24
0.285CysTrp: 0.285 ± 0.147
0.498CysTyr: 0.498 ± 0.207
0.0CysXaa: 0.0 ± 0.0
Asp
4.629AspAla: 4.629 ± 0.687
1.282AspCys: 1.282 ± 0.213
3.774AspAsp: 3.774 ± 0.48
5.127AspGlu: 5.127 ± 0.674
3.133AspPhe: 3.133 ± 0.45
6.551AspGly: 6.551 ± 0.706
0.712AspHis: 0.712 ± 0.236
5.768AspIle: 5.768 ± 0.637
5.412AspLys: 5.412 ± 0.59
4.486AspLeu: 4.486 ± 0.652
2.208AspMet: 2.208 ± 0.367
3.418AspAsn: 3.418 ± 0.472
1.495AspPro: 1.495 ± 0.332
1.353AspGln: 1.353 ± 0.321
2.279AspArg: 2.279 ± 0.467
3.276AspSer: 3.276 ± 0.377
3.276AspThr: 3.276 ± 0.416
3.347AspVal: 3.347 ± 0.438
0.926AspTrp: 0.926 ± 0.291
3.062AspTyr: 3.062 ± 0.457
0.0AspXaa: 0.0 ± 0.0
Glu
4.059GluAla: 4.059 ± 0.485
0.997GluCys: 0.997 ± 0.251
4.201GluAsp: 4.201 ± 0.581
8.047GluGlu: 8.047 ± 1.125
2.848GluPhe: 2.848 ± 0.42
2.92GluGly: 2.92 ± 0.388
1.495GluHis: 1.495 ± 0.299
5.341GluIle: 5.341 ± 0.621
7.904GluLys: 7.904 ± 0.916
6.694GluLeu: 6.694 ± 0.715
3.917GluMet: 3.917 ± 0.58
5.412GluAsn: 5.412 ± 0.662
1.567GluPro: 1.567 ± 0.455
3.133GluGln: 3.133 ± 0.478
4.201GluArg: 4.201 ± 0.7
5.127GluSer: 5.127 ± 0.569
4.913GluThr: 4.913 ± 0.53
4.771GluVal: 4.771 ± 0.626
1.282GluTrp: 1.282 ± 0.343
3.347GluTyr: 3.347 ± 0.472
0.0GluXaa: 0.0 ± 0.0
Phe
2.421PheAla: 2.421 ± 0.407
0.712PheCys: 0.712 ± 0.261
2.635PheAsp: 2.635 ± 0.571
2.777PheGlu: 2.777 ± 0.448
1.567PhePhe: 1.567 ± 0.273
2.564PheGly: 2.564 ± 0.444
0.641PheHis: 0.641 ± 0.272
2.279PheIle: 2.279 ± 0.386
3.917PheLys: 3.917 ± 0.523
3.632PheLeu: 3.632 ± 0.477
1.282PheMet: 1.282 ± 0.326
2.136PheAsn: 2.136 ± 0.419
1.139PhePro: 1.139 ± 0.294
0.926PheGln: 0.926 ± 0.217
1.068PheArg: 1.068 ± 0.298
2.136PheSer: 2.136 ± 0.337
1.994PheThr: 1.994 ± 0.442
2.35PheVal: 2.35 ± 0.41
0.356PheTrp: 0.356 ± 0.148
1.638PheTyr: 1.638 ± 0.357
0.0PheXaa: 0.0 ± 0.0
Gly
3.703GlyAla: 3.703 ± 0.472
0.57GlyCys: 0.57 ± 0.201
3.347GlyAsp: 3.347 ± 0.426
4.201GlyGlu: 4.201 ± 0.545
3.489GlyPhe: 3.489 ± 0.428
5.27GlyGly: 5.27 ± 0.657
1.211GlyHis: 1.211 ± 0.269
5.697GlyIle: 5.697 ± 0.593
6.266GlyLys: 6.266 ± 0.906
4.13GlyLeu: 4.13 ± 0.512
1.851GlyMet: 1.851 ± 0.462
3.845GlyAsn: 3.845 ± 0.603
0.214GlyPro: 0.214 ± 0.103
1.923GlyGln: 1.923 ± 0.389
3.774GlyArg: 3.774 ± 0.488
3.917GlySer: 3.917 ± 0.669
3.917GlyThr: 3.917 ± 0.644
4.486GlyVal: 4.486 ± 0.522
0.997GlyTrp: 0.997 ± 0.285
3.347GlyTyr: 3.347 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
0.926HisAla: 0.926 ± 0.292
0.57HisCys: 0.57 ± 0.223
0.57HisAsp: 0.57 ± 0.213
2.279HisGlu: 2.279 ± 0.439
0.926HisPhe: 0.926 ± 0.224
1.139HisGly: 1.139 ± 0.307
0.498HisHis: 0.498 ± 0.168
1.282HisIle: 1.282 ± 0.273
1.139HisLys: 1.139 ± 0.279
0.997HisLeu: 0.997 ± 0.305
0.57HisMet: 0.57 ± 0.204
0.712HisAsn: 0.712 ± 0.193
0.926HisPro: 0.926 ± 0.251
0.285HisGln: 0.285 ± 0.146
0.356HisArg: 0.356 ± 0.157
1.211HisSer: 1.211 ± 0.31
0.997HisThr: 0.997 ± 0.294
0.712HisVal: 0.712 ± 0.232
0.427HisTrp: 0.427 ± 0.161
0.641HisTyr: 0.641 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.198IleAla: 5.198 ± 0.515
1.353IleCys: 1.353 ± 0.321
5.341IleAsp: 5.341 ± 0.606
5.056IleGlu: 5.056 ± 0.716
2.421IlePhe: 2.421 ± 0.317
4.913IleGly: 4.913 ± 0.61
0.712IleHis: 0.712 ± 0.242
4.985IleIle: 4.985 ± 0.634
7.05IleLys: 7.05 ± 0.847
4.913IleLeu: 4.913 ± 0.665
1.424IleMet: 1.424 ± 0.35
4.273IleAsn: 4.273 ± 0.656
2.065IlePro: 2.065 ± 0.403
2.991IleGln: 2.991 ± 0.42
3.774IleArg: 3.774 ± 0.451
5.056IleSer: 5.056 ± 0.54
5.412IleThr: 5.412 ± 0.765
4.415IleVal: 4.415 ± 0.613
0.498IleTrp: 0.498 ± 0.206
2.279IleTyr: 2.279 ± 0.397
0.0IleXaa: 0.0 ± 0.0
Lys
6.409LysAla: 6.409 ± 0.852
1.068LysCys: 1.068 ± 0.335
4.985LysAsp: 4.985 ± 0.631
7.05LysGlu: 7.05 ± 0.757
2.136LysPhe: 2.136 ± 0.418
4.913LysGly: 4.913 ± 0.684
1.495LysHis: 1.495 ± 0.329
6.195LysIle: 6.195 ± 0.716
7.121LysLys: 7.121 ± 0.901
4.557LysLeu: 4.557 ± 0.5
1.923LysMet: 1.923 ± 0.294
5.198LysAsn: 5.198 ± 0.452
3.133LysPro: 3.133 ± 0.563
3.489LysGln: 3.489 ± 0.466
3.774LysArg: 3.774 ± 0.574
5.056LysSer: 5.056 ± 0.585
4.985LysThr: 4.985 ± 0.493
3.703LysVal: 3.703 ± 0.469
0.855LysTrp: 0.855 ± 0.181
3.917LysTyr: 3.917 ± 0.545
0.0LysXaa: 0.0 ± 0.0
Leu
4.273LeuAla: 4.273 ± 0.676
1.567LeuCys: 1.567 ± 0.388
4.842LeuAsp: 4.842 ± 0.548
5.697LeuGlu: 5.697 ± 0.761
2.635LeuPhe: 2.635 ± 0.444
3.703LeuGly: 3.703 ± 0.601
0.997LeuHis: 0.997 ± 0.293
5.839LeuIle: 5.839 ± 0.7
5.768LeuLys: 5.768 ± 0.769
3.917LeuLeu: 3.917 ± 0.637
1.567LeuMet: 1.567 ± 0.334
4.13LeuAsn: 4.13 ± 0.437
2.706LeuPro: 2.706 ± 0.36
2.706LeuGln: 2.706 ± 0.514
2.92LeuArg: 2.92 ± 0.48
4.486LeuSer: 4.486 ± 0.701
4.13LeuThr: 4.13 ± 0.447
4.13LeuVal: 4.13 ± 0.466
0.712LeuTrp: 0.712 ± 0.197
3.418LeuTyr: 3.418 ± 0.501
0.0LeuXaa: 0.0 ± 0.0
Met
2.208MetAla: 2.208 ± 0.381
0.214MetCys: 0.214 ± 0.14
1.851MetAsp: 1.851 ± 0.359
2.35MetGlu: 2.35 ± 0.443
1.139MetPhe: 1.139 ± 0.376
2.065MetGly: 2.065 ± 0.418
0.641MetHis: 0.641 ± 0.194
2.279MetIle: 2.279 ± 0.359
2.777MetLys: 2.777 ± 0.407
2.421MetLeu: 2.421 ± 0.533
0.57MetMet: 0.57 ± 0.188
2.136MetAsn: 2.136 ± 0.337
1.495MetPro: 1.495 ± 0.356
1.282MetGln: 1.282 ± 0.282
0.712MetArg: 0.712 ± 0.189
2.421MetSer: 2.421 ± 0.44
1.709MetThr: 1.709 ± 0.352
1.495MetVal: 1.495 ± 0.298
0.071MetTrp: 0.071 ± 0.069
0.641MetTyr: 0.641 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
4.273AsnAla: 4.273 ± 0.501
1.211AsnCys: 1.211 ± 0.278
2.492AsnAsp: 2.492 ± 0.393
3.56AsnGlu: 3.56 ± 0.521
1.567AsnPhe: 1.567 ± 0.299
5.056AsnGly: 5.056 ± 0.633
1.424AsnHis: 1.424 ± 0.415
3.56AsnIle: 3.56 ± 0.463
3.489AsnLys: 3.489 ± 0.69
3.632AsnLeu: 3.632 ± 0.498
1.994AsnMet: 1.994 ± 0.333
2.492AsnAsn: 2.492 ± 0.403
2.136AsnPro: 2.136 ± 0.312
1.994AsnGln: 1.994 ± 0.409
2.564AsnArg: 2.564 ± 0.421
3.133AsnSer: 3.133 ± 0.459
3.632AsnThr: 3.632 ± 0.49
3.917AsnVal: 3.917 ± 0.534
0.356AsnTrp: 0.356 ± 0.186
2.136AsnTyr: 2.136 ± 0.315
0.0AsnXaa: 0.0 ± 0.0
Pro
1.567ProAla: 1.567 ± 0.242
0.427ProCys: 0.427 ± 0.194
3.062ProAsp: 3.062 ± 0.502
2.564ProGlu: 2.564 ± 0.542
1.424ProPhe: 1.424 ± 0.28
0.783ProGly: 0.783 ± 0.218
0.57ProHis: 0.57 ± 0.246
1.78ProIle: 1.78 ± 0.388
2.136ProLys: 2.136 ± 0.356
2.421ProLeu: 2.421 ± 0.456
0.498ProMet: 0.498 ± 0.138
1.709ProAsn: 1.709 ± 0.352
0.285ProPro: 0.285 ± 0.117
0.926ProGln: 0.926 ± 0.304
1.139ProArg: 1.139 ± 0.375
1.638ProSer: 1.638 ± 0.45
2.136ProThr: 2.136 ± 0.533
1.567ProVal: 1.567 ± 0.294
0.071ProTrp: 0.071 ± 0.06
1.353ProTyr: 1.353 ± 0.307
0.0ProXaa: 0.0 ± 0.0
Gln
1.851GlnAla: 1.851 ± 0.357
0.712GlnCys: 0.712 ± 0.204
1.78GlnAsp: 1.78 ± 0.27
2.279GlnGlu: 2.279 ± 0.381
1.567GlnPhe: 1.567 ± 0.313
1.923GlnGly: 1.923 ± 0.325
0.641GlnHis: 0.641 ± 0.214
3.204GlnIle: 3.204 ± 0.449
2.492GlnLys: 2.492 ± 0.445
2.635GlnLeu: 2.635 ± 0.323
1.709GlnMet: 1.709 ± 0.316
1.638GlnAsn: 1.638 ± 0.354
0.926GlnPro: 0.926 ± 0.223
2.136GlnGln: 2.136 ± 0.458
1.567GlnArg: 1.567 ± 0.486
1.851GlnSer: 1.851 ± 0.365
2.92GlnThr: 2.92 ± 0.623
1.068GlnVal: 1.068 ± 0.31
0.641GlnTrp: 0.641 ± 0.197
2.421GlnTyr: 2.421 ± 0.333
0.0GlnXaa: 0.0 ± 0.0
Arg
2.706ArgAla: 2.706 ± 0.474
0.855ArgCys: 0.855 ± 0.256
2.35ArgAsp: 2.35 ± 0.419
2.991ArgGlu: 2.991 ± 0.401
1.709ArgPhe: 1.709 ± 0.373
2.136ArgGly: 2.136 ± 0.493
0.641ArgHis: 0.641 ± 0.236
3.703ArgIle: 3.703 ± 0.622
3.703ArgLys: 3.703 ± 0.605
2.991ArgLeu: 2.991 ± 0.468
0.997ArgMet: 0.997 ± 0.273
1.78ArgAsn: 1.78 ± 0.307
1.139ArgPro: 1.139 ± 0.289
1.282ArgGln: 1.282 ± 0.321
2.35ArgArg: 2.35 ± 0.512
2.564ArgSer: 2.564 ± 0.329
2.421ArgThr: 2.421 ± 0.404
2.279ArgVal: 2.279 ± 0.446
0.783ArgTrp: 0.783 ± 0.226
1.994ArgTyr: 1.994 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
4.059SerAla: 4.059 ± 0.822
0.926SerCys: 0.926 ± 0.262
5.198SerAsp: 5.198 ± 0.683
5.127SerGlu: 5.127 ± 0.744
3.632SerPhe: 3.632 ± 0.424
4.629SerGly: 4.629 ± 0.674
0.997SerHis: 0.997 ± 0.257
4.201SerIle: 4.201 ± 0.726
4.913SerLys: 4.913 ± 0.473
4.913SerLeu: 4.913 ± 0.569
1.923SerMet: 1.923 ± 0.321
2.777SerAsn: 2.777 ± 0.448
0.997SerPro: 0.997 ± 0.264
1.851SerGln: 1.851 ± 0.378
1.567SerArg: 1.567 ± 0.303
4.557SerSer: 4.557 ± 0.785
2.777SerThr: 2.777 ± 0.556
3.347SerVal: 3.347 ± 0.57
0.712SerTrp: 0.712 ± 0.255
2.421SerTyr: 2.421 ± 0.408
0.0SerXaa: 0.0 ± 0.0
Thr
4.985ThrAla: 4.985 ± 0.727
0.926ThrCys: 0.926 ± 0.265
3.489ThrAsp: 3.489 ± 0.59
4.7ThrGlu: 4.7 ± 0.618
1.495ThrPhe: 1.495 ± 0.225
5.127ThrGly: 5.127 ± 0.733
0.712ThrHis: 0.712 ± 0.231
5.127ThrIle: 5.127 ± 0.648
2.92ThrLys: 2.92 ± 0.378
3.988ThrLeu: 3.988 ± 0.589
1.78ThrMet: 1.78 ± 0.342
2.564ThrAsn: 2.564 ± 0.435
2.848ThrPro: 2.848 ± 0.456
2.635ThrGln: 2.635 ± 0.474
2.065ThrArg: 2.065 ± 0.376
3.703ThrSer: 3.703 ± 0.651
3.418ThrThr: 3.418 ± 0.56
4.771ThrVal: 4.771 ± 0.57
0.427ThrTrp: 0.427 ± 0.171
2.279ThrTyr: 2.279 ± 0.36
0.0ThrXaa: 0.0 ± 0.0
Val
3.703ValAla: 3.703 ± 0.526
0.855ValCys: 0.855 ± 0.311
4.557ValAsp: 4.557 ± 0.52
5.341ValGlu: 5.341 ± 0.631
1.709ValPhe: 1.709 ± 0.385
2.92ValGly: 2.92 ± 0.485
1.068ValHis: 1.068 ± 0.297
3.845ValIle: 3.845 ± 0.55
3.703ValLys: 3.703 ± 0.518
4.201ValLeu: 4.201 ± 0.57
1.851ValMet: 1.851 ± 0.422
2.492ValAsn: 2.492 ± 0.494
1.638ValPro: 1.638 ± 0.374
1.709ValGln: 1.709 ± 0.386
3.133ValArg: 3.133 ± 0.566
4.201ValSer: 4.201 ± 0.51
3.774ValThr: 3.774 ± 0.589
2.635ValVal: 2.635 ± 0.436
1.139ValTrp: 1.139 ± 0.277
2.065ValTyr: 2.065 ± 0.415
0.0ValXaa: 0.0 ± 0.0
Trp
0.641TrpAla: 0.641 ± 0.213
0.214TrpCys: 0.214 ± 0.137
0.783TrpAsp: 0.783 ± 0.193
1.068TrpGlu: 1.068 ± 0.287
0.712TrpPhe: 0.712 ± 0.226
1.139TrpGly: 1.139 ± 0.323
0.356TrpHis: 0.356 ± 0.142
0.498TrpIle: 0.498 ± 0.187
0.997TrpLys: 0.997 ± 0.249
0.997TrpLeu: 0.997 ± 0.259
0.712TrpMet: 0.712 ± 0.202
1.068TrpAsn: 1.068 ± 0.306
0.142TrpPro: 0.142 ± 0.092
0.641TrpGln: 0.641 ± 0.241
0.071TrpArg: 0.071 ± 0.076
0.641TrpSer: 0.641 ± 0.272
0.57TrpThr: 0.57 ± 0.159
0.214TrpVal: 0.214 ± 0.112
0.071TrpTrp: 0.071 ± 0.076
0.498TrpTyr: 0.498 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.421TyrAla: 2.421 ± 0.376
0.926TyrCys: 0.926 ± 0.247
2.706TyrAsp: 2.706 ± 0.475
3.845TyrGlu: 3.845 ± 0.593
1.638TyrPhe: 1.638 ± 0.373
3.204TyrGly: 3.204 ± 0.436
0.712TyrHis: 0.712 ± 0.241
2.848TyrIle: 2.848 ± 0.339
3.276TyrLys: 3.276 ± 0.477
3.133TyrLeu: 3.133 ± 0.546
1.353TyrMet: 1.353 ± 0.336
2.35TyrAsn: 2.35 ± 0.324
1.068TyrPro: 1.068 ± 0.296
1.424TyrGln: 1.424 ± 0.297
1.495TyrArg: 1.495 ± 0.32
1.923TyrSer: 1.923 ± 0.403
2.848TyrThr: 2.848 ± 0.622
2.92TyrVal: 2.92 ± 0.345
0.427TyrTrp: 0.427 ± 0.221
2.492TyrTyr: 2.492 ± 0.445
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (14044 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski