Ureaplasma diversum NCTC 246
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 666 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A084EZE9|A0A084EZE9_9MOLU Uncharacterized protein OS=Ureaplasma diversum NCTC 246 OX=1188241 GN=UDIV_3550 PE=4 SV=1
MM1 pKa = 7.45 EE2 pKa = 5.21 NWNEE6 pKa = 3.83 WKK8 pKa = 10.34 EE9 pKa = 4.15 KK10 pKa = 10.14 QDD12 pKa = 3.79 EE13 pKa = 4.22 QTLAHH18 pKa = 6.91 LNYY21 pKa = 9.73 ISNLIDD27 pKa = 3.86 DD28 pKa = 4.6 NKK30 pKa = 11.15 NLFEE34 pKa = 4.55 YY35 pKa = 10.39 ILRR38 pKa = 11.84 NFNYY42 pKa = 9.87 QFYY45 pKa = 9.13 TLTKK49 pKa = 9.58 NANILSKK56 pKa = 10.82 SVFLFLVHH64 pKa = 6.05 NQEE67 pKa = 4.63 LYY69 pKa = 10.94 GSFLQSHH76 pKa = 6.36 TEE78 pKa = 4.17 TINSIEE84 pKa = 4.05 NDD86 pKa = 3.23 NNIDD90 pKa = 3.73 FVEE93 pKa = 4.0 VDD95 pKa = 3.13 EE96 pKa = 5.99 DD97 pKa = 3.96 EE98 pKa = 4.82 NEE100 pKa = 4.05 LYY102 pKa = 10.41 LYY104 pKa = 10.79 SLEE107 pKa = 4.04 QVIDD111 pKa = 4.1 FFIQDD116 pKa = 3.5 EE117 pKa = 4.74 FEE119 pKa = 4.32 LSRR122 pKa = 11.84 EE123 pKa = 4.1 EE124 pKa = 3.85 IKK126 pKa = 10.01 TYY128 pKa = 9.78 ATIKK132 pKa = 10.4 IEE134 pKa = 3.88
Molecular weight: 16.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.155
IPC2_protein 4.215
IPC_protein 4.126
Toseland 3.973
ProMoST 4.202
Dawson 4.075
Bjellqvist 4.266
Wikipedia 3.935
Rodwell 3.961
Grimsley 3.884
Solomon 4.062
Lehninger 4.024
Nozaki 4.19
DTASelect 4.304
Thurlkill 3.973
EMBOSS 3.961
Sillero 4.24
Patrickios 3.083
IPC_peptide 4.075
IPC2_peptide 4.228
IPC2.peptide.svr19 4.15
Protein with the highest isoelectric point:
>tr|A0A084EZQ4|A0A084EZQ4_9MOLU 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase OS=Ureaplasma diversum NCTC 246 OX=1188241 GN=UDIV_2890 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPNNRR10 pKa = 11.84 KK11 pKa = 8.78 RR12 pKa = 11.84 AKK14 pKa = 9.29 VHH16 pKa = 5.53 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 8.71 TKK25 pKa = 10.15 NGRR28 pKa = 11.84 IVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.53 GRR39 pKa = 11.84 HH40 pKa = 5.44 SLTVSSEE47 pKa = 3.91 RR48 pKa = 3.86
Molecular weight: 5.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
666
0
666
252712
37
3316
379.4
43.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.763 ± 0.086
0.672 ± 0.032
5.928 ± 0.074
6.173 ± 0.117
4.747 ± 0.08
4.261 ± 0.091
1.641 ± 0.04
8.609 ± 0.115
9.742 ± 0.11
9.518 ± 0.097
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.595 ± 0.043
8.231 ± 0.104
2.965 ± 0.059
4.287 ± 0.063
2.89 ± 0.065
6.564 ± 0.071
5.416 ± 0.074
5.938 ± 0.072
0.851 ± 0.026
4.21 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here