Actinoplanes utahensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales;

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A6U9X8|A0A0A6U9X8_ACTUT 2 3-bisphosphoglycerate-dependent phosphoglycerate mutase OS=Actinoplanes utahensis OX=1869 GN=gpmA PE=3 SV=1
MM1 pKa = 7.54WEE3 pKa = 3.85VDD5 pKa = 3.55VAADD9 pKa = 3.7TDD11 pKa = 4.26EE12 pKa = 4.45LQVWIDD18 pKa = 3.4QDD20 pKa = 4.0LCTGDD25 pKa = 3.99GLCVQYY31 pKa = 11.02APEE34 pKa = 4.2VFEE37 pKa = 5.9LDD39 pKa = 3.05IDD41 pKa = 3.71GLAYY45 pKa = 10.81VKK47 pKa = 10.65DD48 pKa = 3.83HH49 pKa = 6.71SGEE52 pKa = 4.14LLQSPGVRR60 pKa = 11.84TLVPSGLLLEE70 pKa = 5.22VIDD73 pKa = 4.93SAKK76 pKa = 10.16EE77 pKa = 3.88CPGDD81 pKa = 4.12CIHH84 pKa = 6.79VVRR87 pKa = 11.84TADD90 pKa = 3.56DD91 pKa = 3.72VAVAGPDD98 pKa = 3.21AHH100 pKa = 7.1EE101 pKa = 4.61GTT103 pKa = 4.55

Molecular weight: 11.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A6UGC2|A0A0A6UGC2_ACTUT Citrate synthase (unknown stereospecificity) OS=Actinoplanes utahensis OX=1869 GN=MB27_24570 PE=4 SV=1
MM1 pKa = 7.29AVSVPVVAAALVAVSVPVVAAALVAASVPVVAAALVAVTGVVPVAAPVARR51 pKa = 11.84SAPALRR57 pKa = 11.84VVPVAAVPVAVAVAVARR74 pKa = 11.84RR75 pKa = 11.84VPSGVPAAGRR85 pKa = 11.84RR86 pKa = 11.84VAASRR91 pKa = 11.84RR92 pKa = 11.84SRR94 pKa = 11.84GVRR97 pKa = 11.84SSTTCPLRR105 pKa = 11.84RR106 pKa = 3.7

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7275

0

7275

2280864

34

6670

313.5

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.777 ± 0.046

0.703 ± 0.009

6.083 ± 0.022

5.222 ± 0.029

2.838 ± 0.017

9.266 ± 0.026

2.152 ± 0.016

3.756 ± 0.022

1.917 ± 0.022

10.337 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.766 ± 0.011

1.879 ± 0.018

6.059 ± 0.028

2.69 ± 0.018

8.069 ± 0.037

4.917 ± 0.021

6.283 ± 0.033

8.652 ± 0.024

1.561 ± 0.012

2.073 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski