Thermoflavifilum thermophilum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Thermoflavifilum

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7N0J3|A0A1I7N0J3_9BACT Uridine phosphorylase OS=Thermoflavifilum thermophilum OX=1393122 GN=SAMN05660895_0280 PE=4 SV=1
MM1 pKa = 7.82VIDD4 pKa = 4.77DD5 pKa = 3.75QAGLQDD11 pKa = 4.21QIIAVLKK18 pKa = 9.09TVYY21 pKa = 10.6DD22 pKa = 3.56PEE24 pKa = 5.22IPVNIYY30 pKa = 10.87DD31 pKa = 4.0LGLVYY36 pKa = 10.12EE37 pKa = 4.03LHH39 pKa = 6.65INQEE43 pKa = 4.18GEE45 pKa = 4.15VFILVTLTAPGCPVGADD62 pKa = 2.83IVSEE66 pKa = 3.76IDD68 pKa = 3.26EE69 pKa = 4.38KK70 pKa = 11.09VRR72 pKa = 11.84QIEE75 pKa = 4.3GVKK78 pKa = 10.16DD79 pKa = 3.19VHH81 pKa = 8.24VEE83 pKa = 4.05LTFDD87 pKa = 4.12PPWNMGMMSDD97 pKa = 3.81EE98 pKa = 4.82AKK100 pKa = 10.96LEE102 pKa = 4.25LGFMM106 pKa = 4.56

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7N1H0|A0A1I7N1H0_9BACT GH3 auxin-responsive promoter OS=Thermoflavifilum thermophilum OX=1393122 GN=SAMN05660895_0340 PE=4 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84PKK5 pKa = 10.47KK6 pKa = 10.16RR7 pKa = 11.84ATRR10 pKa = 11.84KK11 pKa = 9.33AASKK15 pKa = 10.47KK16 pKa = 9.82KK17 pKa = 9.82AATRR21 pKa = 11.84KK22 pKa = 9.58KK23 pKa = 9.81RR24 pKa = 11.84ATRR27 pKa = 11.84KK28 pKa = 9.12KK29 pKa = 9.3AAVRR33 pKa = 11.84KK34 pKa = 9.25KK35 pKa = 10.22AARR38 pKa = 11.84GKK40 pKa = 10.28KK41 pKa = 9.15KK42 pKa = 10.18AASRR46 pKa = 11.84KK47 pKa = 9.03KK48 pKa = 9.82AATRR52 pKa = 11.84KK53 pKa = 9.59KK54 pKa = 9.81RR55 pKa = 11.84ATRR58 pKa = 11.84KK59 pKa = 9.39KK60 pKa = 9.57AATRR64 pKa = 11.84KK65 pKa = 9.27KK66 pKa = 9.91AAGRR70 pKa = 11.84KK71 pKa = 8.61KK72 pKa = 10.23AAAKK76 pKa = 10.11KK77 pKa = 9.86KK78 pKa = 9.86AATRR82 pKa = 11.84KK83 pKa = 9.42KK84 pKa = 10.23AATRR88 pKa = 11.84KK89 pKa = 9.66KK90 pKa = 9.75KK91 pKa = 10.3AAVRR95 pKa = 11.84KK96 pKa = 9.26KK97 pKa = 10.37RR98 pKa = 11.84AMPKK102 pKa = 9.53NAGNGTTGTATGPAEE117 pKa = 4.51GMNSTPPLMPP127 pKa = 4.85

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2347

0

2347

879158

38

2390

374.6

42.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.834 ± 0.052

0.995 ± 0.015

5.081 ± 0.032

5.324 ± 0.056

4.629 ± 0.033

6.603 ± 0.04

2.96 ± 0.028

7.015 ± 0.044

4.863 ± 0.045

9.978 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.02

4.107 ± 0.049

4.77 ± 0.026

5.273 ± 0.042

5.114 ± 0.037

5.71 ± 0.04

5.165 ± 0.04

6.247 ± 0.035

1.601 ± 0.023

4.251 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski