Amino acid dipepetide frequency for Thermoflavifilum thermophilum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.999AlaAla: 6.999 ± 0.124
0.94AlaCys: 0.94 ± 0.035
4.447AlaAsp: 4.447 ± 0.074
4.375AlaGlu: 4.375 ± 0.083
3.803AlaPhe: 3.803 ± 0.078
5.991AlaGly: 5.991 ± 0.096
2.195AlaHis: 2.195 ± 0.055
5.327AlaIle: 5.327 ± 0.092
2.738AlaLys: 2.738 ± 0.067
7.687AlaLeu: 7.687 ± 0.117
2.041AlaMet: 2.041 ± 0.056
2.74AlaAsn: 2.74 ± 0.069
2.701AlaPro: 2.701 ± 0.054
3.685AlaGln: 3.685 ± 0.071
4.394AlaArg: 4.394 ± 0.088
4.649AlaSer: 4.649 ± 0.079
3.905AlaThr: 3.905 ± 0.075
5.017AlaVal: 5.017 ± 0.076
1.21AlaTrp: 1.21 ± 0.038
3.497AlaTyr: 3.497 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.654CysAla: 0.654 ± 0.027
0.205CysCys: 0.205 ± 0.013
0.412CysAsp: 0.412 ± 0.022
0.494CysGlu: 0.494 ± 0.022
0.513CysPhe: 0.513 ± 0.026
0.803CysGly: 0.803 ± 0.033
0.298CysHis: 0.298 ± 0.019
0.792CysIle: 0.792 ± 0.028
0.398CysLys: 0.398 ± 0.023
1.026CysLeu: 1.026 ± 0.039
0.303CysMet: 0.303 ± 0.016
0.376CysAsn: 0.376 ± 0.021
0.412CysPro: 0.412 ± 0.027
0.367CysGln: 0.367 ± 0.026
0.475CysArg: 0.475 ± 0.023
0.591CysSer: 0.591 ± 0.031
0.558CysThr: 0.558 ± 0.03
0.651CysVal: 0.651 ± 0.029
0.208CysTrp: 0.208 ± 0.016
0.416CysTyr: 0.416 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.126AspAla: 4.126 ± 0.079
0.375AspCys: 0.375 ± 0.02
2.327AspAsp: 2.327 ± 0.064
3.227AspGlu: 3.227 ± 0.07
2.577AspPhe: 2.577 ± 0.051
3.224AspGly: 3.224 ± 0.085
1.318AspHis: 1.318 ± 0.042
3.644AspIle: 3.644 ± 0.058
2.076AspLys: 2.076 ± 0.058
4.692AspLeu: 4.692 ± 0.081
1.225AspMet: 1.225 ± 0.037
1.695AspAsn: 1.695 ± 0.049
2.683AspPro: 2.683 ± 0.061
2.259AspGln: 2.259 ± 0.053
2.337AspArg: 2.337 ± 0.047
3.29AspSer: 3.29 ± 0.088
3.092AspThr: 3.092 ± 0.078
3.419AspVal: 3.419 ± 0.066
0.831AspTrp: 0.831 ± 0.032
2.394AspTyr: 2.394 ± 0.051
0.0AspXaa: 0.0 ± 0.0
Glu
4.611GluAla: 4.611 ± 0.097
0.358GluCys: 0.358 ± 0.021
2.394GluAsp: 2.394 ± 0.051
3.738GluGlu: 3.738 ± 0.113
1.84GluPhe: 1.84 ± 0.051
3.098GluGly: 3.098 ± 0.067
1.25GluHis: 1.25 ± 0.036
4.105GluIle: 4.105 ± 0.084
4.219GluLys: 4.219 ± 0.096
5.088GluLeu: 5.088 ± 0.087
1.462GluMet: 1.462 ± 0.043
2.523GluAsn: 2.523 ± 0.055
1.764GluPro: 1.764 ± 0.047
3.065GluGln: 3.065 ± 0.068
2.606GluArg: 2.606 ± 0.064
2.395GluSer: 2.395 ± 0.059
2.606GluThr: 2.606 ± 0.061
3.381GluVal: 3.381 ± 0.07
0.885GluTrp: 0.885 ± 0.033
1.853GluTyr: 1.853 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
3.365PheAla: 3.365 ± 0.067
0.579PheCys: 0.579 ± 0.026
2.602PheAsp: 2.602 ± 0.064
2.167PheGlu: 2.167 ± 0.056
2.307PhePhe: 2.307 ± 0.074
2.905PheGly: 2.905 ± 0.063
1.479PheHis: 1.479 ± 0.043
3.209PheIle: 3.209 ± 0.064
1.732PheLys: 1.732 ± 0.051
4.484PheLeu: 4.484 ± 0.09
1.012PheMet: 1.012 ± 0.033
1.865PheAsn: 1.865 ± 0.049
2.295PhePro: 2.295 ± 0.053
2.209PheGln: 2.209 ± 0.055
2.467PheArg: 2.467 ± 0.057
3.277PheSer: 3.277 ± 0.067
2.935PheThr: 2.935 ± 0.055
2.7PheVal: 2.7 ± 0.057
0.709PheTrp: 0.709 ± 0.033
1.992PheTyr: 1.992 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
4.31GlyAla: 4.31 ± 0.088
0.857GlyCys: 0.857 ± 0.04
3.188GlyAsp: 3.188 ± 0.068
3.194GlyGlu: 3.194 ± 0.061
3.276GlyPhe: 3.276 ± 0.072
4.561GlyGly: 4.561 ± 0.101
1.65GlyHis: 1.65 ± 0.047
5.253GlyIle: 5.253 ± 0.071
4.369GlyLys: 4.369 ± 0.065
6.314GlyLeu: 6.314 ± 0.092
2.036GlyMet: 2.036 ± 0.051
3.046GlyAsn: 3.046 ± 0.086
1.865GlyPro: 1.865 ± 0.055
2.664GlyGln: 2.664 ± 0.055
3.047GlyArg: 3.047 ± 0.063
3.783GlySer: 3.783 ± 0.074
3.597GlyThr: 3.597 ± 0.088
4.239GlyVal: 4.239 ± 0.079
1.409GlyTrp: 1.409 ± 0.048
3.375GlyTyr: 3.375 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
2.289HisAla: 2.289 ± 0.056
0.278HisCys: 0.278 ± 0.02
1.391HisAsp: 1.391 ± 0.05
1.448HisGlu: 1.448 ± 0.044
1.696HisPhe: 1.696 ± 0.048
1.594HisGly: 1.594 ± 0.048
1.185HisHis: 1.185 ± 0.039
2.195HisIle: 2.195 ± 0.057
0.984HisLys: 0.984 ± 0.036
3.038HisLeu: 3.038 ± 0.063
0.601HisMet: 0.601 ± 0.025
0.903HisAsn: 0.903 ± 0.036
2.165HisPro: 2.165 ± 0.056
1.789HisGln: 1.789 ± 0.053
1.587HisArg: 1.587 ± 0.047
1.343HisSer: 1.343 ± 0.036
1.581HisThr: 1.581 ± 0.046
1.804HisVal: 1.804 ± 0.05
0.46HisTrp: 0.46 ± 0.025
1.273HisTyr: 1.273 ± 0.038
0.0HisXaa: 0.0 ± 0.0
Ile
5.839IleAla: 5.839 ± 0.099
0.965IleCys: 0.965 ± 0.03
3.641IleAsp: 3.641 ± 0.072
3.66IleGlu: 3.66 ± 0.073
3.081IlePhe: 3.081 ± 0.063
4.585IleGly: 4.585 ± 0.091
2.169IleHis: 2.169 ± 0.044
4.427IleIle: 4.427 ± 0.091
2.839IleLys: 2.839 ± 0.067
6.499IleLeu: 6.499 ± 0.112
1.329IleMet: 1.329 ± 0.038
2.731IleAsn: 2.731 ± 0.061
3.988IlePro: 3.988 ± 0.071
3.481IleGln: 3.481 ± 0.073
4.632IleArg: 4.632 ± 0.07
4.598IleSer: 4.598 ± 0.083
3.926IleThr: 3.926 ± 0.066
4.171IleVal: 4.171 ± 0.07
0.984IleTrp: 0.984 ± 0.037
2.602IleTyr: 2.602 ± 0.061
0.0IleXaa: 0.0 ± 0.0
Lys
3.841LysAla: 3.841 ± 0.073
0.293LysCys: 0.293 ± 0.016
2.426LysAsp: 2.426 ± 0.058
2.85LysGlu: 2.85 ± 0.085
1.561LysPhe: 1.561 ± 0.038
3.243LysGly: 3.243 ± 0.061
1.203LysHis: 1.203 ± 0.034
3.619LysIle: 3.619 ± 0.079
3.483LysLys: 3.483 ± 0.089
4.343LysLeu: 4.343 ± 0.072
1.26LysMet: 1.26 ± 0.039
2.444LysAsn: 2.444 ± 0.06
2.506LysPro: 2.506 ± 0.057
2.742LysGln: 2.742 ± 0.058
2.39LysArg: 2.39 ± 0.054
2.464LysSer: 2.464 ± 0.056
2.596LysThr: 2.596 ± 0.053
2.565LysVal: 2.565 ± 0.062
0.771LysTrp: 0.771 ± 0.029
2.027LysTyr: 2.027 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
7.367LeuAla: 7.367 ± 0.111
1.125LeuCys: 1.125 ± 0.037
4.532LeuAsp: 4.532 ± 0.073
4.652LeuGlu: 4.652 ± 0.097
4.684LeuPhe: 4.684 ± 0.079
5.923LeuGly: 5.923 ± 0.086
3.432LeuHis: 3.432 ± 0.069
6.961LeuIle: 6.961 ± 0.104
5.239LeuLys: 5.239 ± 0.087
10.399LeuLeu: 10.399 ± 0.146
2.59LeuMet: 2.59 ± 0.055
4.33LeuAsn: 4.33 ± 0.081
5.283LeuPro: 5.283 ± 0.084
6.45LeuGln: 6.45 ± 0.111
4.902LeuArg: 4.902 ± 0.089
6.562LeuSer: 6.562 ± 0.093
4.86LeuThr: 4.86 ± 0.08
5.357LeuVal: 5.357 ± 0.092
1.367LeuTrp: 1.367 ± 0.046
3.767LeuTyr: 3.767 ± 0.071
0.0LeuXaa: 0.0 ± 0.0
Met
2.094MetAla: 2.094 ± 0.047
0.172MetCys: 0.172 ± 0.014
1.299MetAsp: 1.299 ± 0.034
1.42MetGlu: 1.42 ± 0.038
0.795MetPhe: 0.795 ± 0.033
1.77MetGly: 1.77 ± 0.047
0.88MetHis: 0.88 ± 0.037
1.657MetIle: 1.657 ± 0.049
1.607MetLys: 1.607 ± 0.042
2.523MetLeu: 2.523 ± 0.059
0.735MetMet: 0.735 ± 0.03
1.106MetAsn: 1.106 ± 0.038
1.342MetPro: 1.342 ± 0.038
1.617MetGln: 1.617 ± 0.048
1.242MetArg: 1.242 ± 0.038
1.217MetSer: 1.217 ± 0.043
0.942MetThr: 0.942 ± 0.028
1.366MetVal: 1.366 ± 0.041
0.28MetTrp: 0.28 ± 0.017
0.735MetTyr: 0.735 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.061AsnAla: 3.061 ± 0.065
0.346AsnCys: 0.346 ± 0.022
2.076AsnAsp: 2.076 ± 0.055
2.075AsnGlu: 2.075 ± 0.049
1.804AsnPhe: 1.804 ± 0.05
3.096AsnGly: 3.096 ± 0.072
1.111AsnHis: 1.111 ± 0.036
2.853AsnIle: 2.853 ± 0.064
1.826AsnLys: 1.826 ± 0.053
3.89AsnLeu: 3.89 ± 0.092
0.921AsnMet: 0.921 ± 0.033
1.896AsnAsn: 1.896 ± 0.065
2.78AsnPro: 2.78 ± 0.063
2.116AsnGln: 2.116 ± 0.057
2.155AsnArg: 2.155 ± 0.05
2.053AsnSer: 2.053 ± 0.062
2.237AsnThr: 2.237 ± 0.067
2.226AsnVal: 2.226 ± 0.057
0.7AsnTrp: 0.7 ± 0.031
1.844AsnTyr: 1.844 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
4.275ProAla: 4.275 ± 0.085
0.351ProCys: 0.351 ± 0.019
3.752ProAsp: 3.752 ± 0.076
3.367ProGlu: 3.367 ± 0.065
2.233ProPhe: 2.233 ± 0.051
3.517ProGly: 3.517 ± 0.067
1.449ProHis: 1.449 ± 0.041
2.585ProIle: 2.585 ± 0.053
1.559ProLys: 1.559 ± 0.039
4.273ProLeu: 4.273 ± 0.074
1.026ProMet: 1.026 ± 0.037
1.832ProAsn: 1.832 ± 0.053
1.935ProPro: 1.935 ± 0.059
2.135ProGln: 2.135 ± 0.055
1.925ProArg: 1.925 ± 0.044
2.564ProSer: 2.564 ± 0.06
2.051ProThr: 2.051 ± 0.052
4.099ProVal: 4.099 ± 0.071
0.655ProTrp: 0.655 ± 0.034
2.117ProTyr: 2.117 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
4.603GlnAla: 4.603 ± 0.073
0.283GlnCys: 0.283 ± 0.018
2.127GlnAsp: 2.127 ± 0.054
2.655GlnGlu: 2.655 ± 0.057
1.995GlnPhe: 1.995 ± 0.046
2.689GlnGly: 2.689 ± 0.053
1.861GlnHis: 1.861 ± 0.054
3.71GlnIle: 3.71 ± 0.073
2.691GlnLys: 2.691 ± 0.058
5.922GlnLeu: 5.922 ± 0.103
1.305GlnMet: 1.305 ± 0.041
1.813GlnAsn: 1.813 ± 0.055
2.688GlnPro: 2.688 ± 0.065
4.435GlnGln: 4.435 ± 0.097
2.501GlnArg: 2.501 ± 0.056
2.514GlnSer: 2.514 ± 0.054
2.599GlnThr: 2.599 ± 0.06
3.379GlnVal: 3.379 ± 0.062
0.842GlnTrp: 0.842 ± 0.035
2.12GlnTyr: 2.12 ± 0.058
0.0GlnXaa: 0.0 ± 0.0
Arg
3.349ArgAla: 3.349 ± 0.07
0.397ArgCys: 0.397 ± 0.024
2.359ArgAsp: 2.359 ± 0.057
3.167ArgGlu: 3.167 ± 0.071
2.647ArgPhe: 2.647 ± 0.053
2.526ArgGly: 2.526 ± 0.063
1.333ArgHis: 1.333 ± 0.038
4.202ArgIle: 4.202 ± 0.07
3.213ArgLys: 3.213 ± 0.067
5.399ArgLeu: 5.399 ± 0.072
1.569ArgMet: 1.569 ± 0.045
2.301ArgAsn: 2.301 ± 0.051
1.967ArgPro: 1.967 ± 0.054
2.865ArgGln: 2.865 ± 0.059
2.635ArgArg: 2.635 ± 0.059
2.431ArgSer: 2.431 ± 0.054
2.231ArgThr: 2.231 ± 0.053
3.129ArgVal: 3.129 ± 0.065
0.95ArgTrp: 0.95 ± 0.038
2.468ArgTyr: 2.468 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
4.469SerAla: 4.469 ± 0.087
0.688SerCys: 0.688 ± 0.027
2.642SerAsp: 2.642 ± 0.051
2.287SerGlu: 2.287 ± 0.056
3.238SerPhe: 3.238 ± 0.069
4.438SerGly: 4.438 ± 0.094
1.553SerHis: 1.553 ± 0.045
3.77SerIle: 3.77 ± 0.07
2.202SerLys: 2.202 ± 0.051
6.287SerLeu: 6.287 ± 0.095
1.449SerMet: 1.449 ± 0.041
2.212SerAsn: 2.212 ± 0.061
2.758SerPro: 2.758 ± 0.06
2.39SerGln: 2.39 ± 0.055
2.94SerArg: 2.94 ± 0.062
3.75SerSer: 3.75 ± 0.076
3.009SerThr: 3.009 ± 0.073
3.555SerVal: 3.555 ± 0.07
0.995SerTrp: 0.995 ± 0.036
2.465SerTyr: 2.465 ± 0.075
0.0SerXaa: 0.0 ± 0.0
Thr
4.118ThrAla: 4.118 ± 0.073
0.477ThrCys: 0.477 ± 0.022
2.847ThrAsp: 2.847 ± 0.065
2.368ThrGlu: 2.368 ± 0.057
2.235ThrPhe: 2.235 ± 0.052
4.463ThrGly: 4.463 ± 0.087
1.523ThrHis: 1.523 ± 0.046
3.607ThrIle: 3.607 ± 0.059
1.649ThrLys: 1.649 ± 0.048
5.367ThrLeu: 5.367 ± 0.09
0.912ThrMet: 0.912 ± 0.035
1.869ThrAsn: 1.869 ± 0.054
2.92ThrPro: 2.92 ± 0.063
2.159ThrGln: 2.159 ± 0.051
2.666ThrArg: 2.666 ± 0.059
3.09ThrSer: 3.09 ± 0.072
3.006ThrThr: 3.006 ± 0.073
3.252ThrVal: 3.252 ± 0.067
0.743ThrTrp: 0.743 ± 0.034
2.375ThrTyr: 2.375 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
4.523ValAla: 4.523 ± 0.094
0.721ValCys: 0.721 ± 0.03
3.346ValAsp: 3.346 ± 0.068
3.227ValGlu: 3.227 ± 0.071
2.944ValPhe: 2.944 ± 0.061
3.8ValGly: 3.8 ± 0.078
1.802ValHis: 1.802 ± 0.051
4.552ValIle: 4.552 ± 0.081
3.406ValLys: 3.406 ± 0.063
6.031ValLeu: 6.031 ± 0.085
1.653ValMet: 1.653 ± 0.047
2.918ValAsn: 2.918 ± 0.069
2.84ValPro: 2.84 ± 0.063
2.914ValGln: 2.914 ± 0.057
2.915ValArg: 2.915 ± 0.06
3.793ValSer: 3.793 ± 0.076
3.001ValThr: 3.001 ± 0.072
4.46ValVal: 4.46 ± 0.087
1.003ValTrp: 1.003 ± 0.04
2.625ValTyr: 2.625 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.924TrpAla: 0.924 ± 0.039
0.147TrpCys: 0.147 ± 0.012
0.75TrpAsp: 0.75 ± 0.037
0.802TrpGlu: 0.802 ± 0.032
0.692TrpPhe: 0.692 ± 0.028
0.976TrpGly: 0.976 ± 0.038
0.469TrpHis: 0.469 ± 0.028
1.388TrpIle: 1.388 ± 0.047
0.97TrpLys: 0.97 ± 0.035
1.887TrpLeu: 1.887 ± 0.059
0.623TrpMet: 0.623 ± 0.027
0.787TrpAsn: 0.787 ± 0.034
0.593TrpPro: 0.593 ± 0.029
0.962TrpGln: 0.962 ± 0.033
0.696TrpArg: 0.696 ± 0.031
0.72TrpSer: 0.72 ± 0.034
0.67TrpThr: 0.67 ± 0.026
1.008TrpVal: 1.008 ± 0.034
0.338TrpTrp: 0.338 ± 0.022
0.61TrpTyr: 0.61 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.526TyrAla: 3.526 ± 0.069
0.397TyrCys: 0.397 ± 0.022
2.252TyrAsp: 2.252 ± 0.047
2.07TyrGlu: 2.07 ± 0.052
2.369TyrPhe: 2.369 ± 0.057
2.831TyrGly: 2.831 ± 0.064
1.441TyrHis: 1.441 ± 0.043
2.291TyrIle: 2.291 ± 0.054
1.428TyrLys: 1.428 ± 0.043
4.636TyrLeu: 4.636 ± 0.081
0.747TyrMet: 0.747 ± 0.031
1.677TyrAsn: 1.677 ± 0.053
2.216TyrPro: 2.216 ± 0.057
2.426TyrGln: 2.426 ± 0.058
2.623TyrArg: 2.623 ± 0.062
2.014TyrSer: 2.014 ± 0.055
2.245TyrThr: 2.245 ± 0.051
2.695TyrVal: 2.695 ± 0.06
0.67TyrTrp: 0.67 ± 0.03
1.951TyrTyr: 1.951 ± 0.06
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2347 proteins (879158 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski