Cotesia congregata bracovirus
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 150 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5ZP31|Q5ZP31_9VIRU Uncharacterized protein OS=Cotesia congregata bracovirus OX=39640 GN=CcBV_10.5 PE=4 SV=1
MM1 pKa = 7.63 VLEE4 pKa = 4.56 VIVPQEE10 pKa = 3.89 WQVLPEE16 pKa = 3.88 ATLRR20 pKa = 11.84 TEE22 pKa = 4.2 LLYY25 pKa = 11.06 LITIQEE31 pKa = 4.33 TQRR34 pKa = 11.84 ATSAFGVYY42 pKa = 10.3 NGVTAKK48 pKa = 10.61 SLEE51 pKa = 4.14 FNGYY55 pKa = 9.12 LWFKK59 pKa = 10.55 KK60 pKa = 10.66 LNDD63 pKa = 3.94 LNEE66 pKa = 4.26 EE67 pKa = 4.15 PDD69 pKa = 3.64 FNIYY73 pKa = 10.14 VLLEE77 pKa = 3.85 IPFIPSSSIQDD88 pKa = 3.7 DD89 pKa = 4.02 DD90 pKa = 4.51 STCSEE95 pKa = 4.58 CDD97 pKa = 3.47 SEE99 pKa = 5.43 QLLSDD104 pKa = 5.13 DD105 pKa = 5.1 EE106 pKa = 4.67 PCSTCDD112 pKa = 3.15 EE113 pKa = 4.43
Molecular weight: 12.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.803
IPC2_protein 3.706
IPC_protein 3.63
Toseland 3.452
ProMoST 3.757
Dawson 3.617
Bjellqvist 3.834
Wikipedia 3.528
Rodwell 3.478
Grimsley 3.363
Solomon 3.592
Lehninger 3.541
Nozaki 3.745
DTASelect 3.884
Thurlkill 3.503
EMBOSS 3.541
Sillero 3.757
Patrickios 0.477
IPC_peptide 3.592
IPC2_peptide 3.732
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|Q5ZP51|Q5ZP51_9VIRU Uncharacterized protein OS=Cotesia congregata bracovirus OX=39640 GN=CcBV_4.1a PE=4 SV=1
MM1 pKa = 7.43 MFRR4 pKa = 11.84 KK5 pKa = 10.03 AGLPTPRR12 pKa = 11.84 APEE15 pKa = 4.21 GRR17 pKa = 11.84 SNTSNQSLVVNRR29 pKa = 11.84 QRR31 pKa = 11.84 DD32 pKa = 3.88 RR33 pKa = 11.84 YY34 pKa = 8.91 PNEE37 pKa = 3.69 AVLSFDD43 pKa = 3.52 VTFLKK48 pKa = 10.82 LGNNSLLITGII59 pKa = 3.78
Molecular weight: 6.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.736
IPC_protein 10.847
Toseland 10.965
ProMoST 10.994
Dawson 11.023
Bjellqvist 10.833
Wikipedia 11.33
Rodwell 11.052
Grimsley 11.067
Solomon 11.301
Lehninger 11.242
Nozaki 10.95
DTASelect 10.833
Thurlkill 10.965
EMBOSS 11.403
Sillero 10.979
Patrickios 10.906
IPC_peptide 11.301
IPC2_peptide 9.97
IPC2.peptide.svr19 8.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
150
0
150
41375
45
1223
275.8
31.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.824 ± 0.129
1.738 ± 0.114
5.051 ± 0.139
6.054 ± 0.16
3.981 ± 0.16
4.831 ± 0.238
2.366 ± 0.08
6.332 ± 0.211
6.364 ± 0.219
7.932 ± 0.195
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.163 ± 0.081
7.234 ± 0.25
4.727 ± 0.248
5.088 ± 0.272
5.097 ± 0.144
8.984 ± 0.255
6.238 ± 0.152
5.979 ± 0.188
0.96 ± 0.063
4.056 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here