Synechococcus phage S-CAM3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Charybdisvirus; Synechococcus virus SCAM3

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8KIV3|A0A1D8KIV3_9CAUD Baseplate wedge subunit OS=Synechococcus phage S-CAM3 OX=1883366 GN=C421010_084 PE=4 SV=1
MM1 pKa = 7.38TDD3 pKa = 3.03KK4 pKa = 11.11EE5 pKa = 4.32FVDD8 pKa = 4.48FLFDD12 pKa = 3.87KK13 pKa = 10.95LVTHH17 pKa = 6.92VDD19 pKa = 2.71TDD21 pKa = 4.03MIDD24 pKa = 3.31LHH26 pKa = 8.55DD27 pKa = 6.07DD28 pKa = 4.08DD29 pKa = 5.39SCCDD33 pKa = 3.42HH34 pKa = 8.14LEE36 pKa = 4.11FEE38 pKa = 4.38QLSLNVTCC46 pKa = 6.12

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8KJA5|A0A1D8KJA5_9CAUD Ribonuclease H OS=Synechococcus phage S-CAM3 OX=1883366 GN=C421010_226 PE=4 SV=1
MM1 pKa = 7.88LIYY4 pKa = 10.18TVFKK8 pKa = 10.68RR9 pKa = 11.84LQSLLFWTGVRR20 pKa = 11.84FPSLPFLGVPWFRR33 pKa = 11.84QGKK36 pKa = 9.26KK37 pKa = 8.28VVIVDD42 pKa = 3.55GTKK45 pKa = 9.94PP46 pKa = 3.09

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

240

0

240

62505

37

3673

260.4

28.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.667 ± 0.239

0.976 ± 0.107

6.833 ± 0.175

6.318 ± 0.205

4.401 ± 0.174

8.18 ± 0.335

1.485 ± 0.095

6.385 ± 0.115

5.526 ± 0.34

7.004 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.925 ± 0.159

5.513 ± 0.199

4.126 ± 0.11

3.565 ± 0.094

3.976 ± 0.113

7.329 ± 0.281

7.431 ± 0.294

6.843 ± 0.129

1.082 ± 0.068

4.436 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski