Amino acid dipepetide frequency for Synechococcus phage S-CAM3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.064AlaAla: 6.064 ± 0.539
0.624AlaCys: 0.624 ± 0.132
3.888AlaAsp: 3.888 ± 0.256
4.144AlaGlu: 4.144 ± 0.377
2.704AlaPhe: 2.704 ± 0.226
6.176AlaGly: 6.176 ± 0.514
0.88AlaHis: 0.88 ± 0.149
3.872AlaIle: 3.872 ± 0.272
3.264AlaLys: 3.264 ± 0.323
4.336AlaLeu: 4.336 ± 0.323
1.28AlaMet: 1.28 ± 0.142
3.328AlaAsn: 3.328 ± 0.249
2.752AlaPro: 2.752 ± 0.264
2.464AlaGln: 2.464 ± 0.215
2.352AlaArg: 2.352 ± 0.169
5.568AlaSer: 5.568 ± 0.382
5.44AlaThr: 5.44 ± 0.414
4.432AlaVal: 4.432 ± 0.305
0.496AlaTrp: 0.496 ± 0.095
2.608AlaTyr: 2.608 ± 0.208
0.0AlaXaa: 0.0 ± 0.0
Cys
0.656CysAla: 0.656 ± 0.136
0.368CysCys: 0.368 ± 0.165
0.864CysAsp: 0.864 ± 0.134
0.768CysGlu: 0.768 ± 0.126
0.32CysPhe: 0.32 ± 0.071
0.976CysGly: 0.976 ± 0.2
0.336CysHis: 0.336 ± 0.098
0.448CysIle: 0.448 ± 0.077
0.48CysLys: 0.48 ± 0.088
0.496CysLeu: 0.496 ± 0.101
0.256CysMet: 0.256 ± 0.07
0.528CysAsn: 0.528 ± 0.114
0.448CysPro: 0.448 ± 0.099
0.304CysGln: 0.304 ± 0.083
0.288CysArg: 0.288 ± 0.082
0.624CysSer: 0.624 ± 0.116
0.448CysThr: 0.448 ± 0.091
0.656CysVal: 0.656 ± 0.104
0.096CysTrp: 0.096 ± 0.044
0.4CysTyr: 0.4 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
4.608AspAla: 4.608 ± 0.305
0.624AspCys: 0.624 ± 0.125
5.072AspAsp: 5.072 ± 0.435
4.176AspGlu: 4.176 ± 0.32
3.376AspPhe: 3.376 ± 0.259
5.6AspGly: 5.6 ± 0.381
0.928AspHis: 0.928 ± 0.13
4.832AspIle: 4.832 ± 0.265
3.504AspLys: 3.504 ± 0.305
5.168AspLeu: 5.168 ± 0.293
1.344AspMet: 1.344 ± 0.173
3.424AspAsn: 3.424 ± 0.299
2.96AspPro: 2.96 ± 0.312
2.016AspGln: 2.016 ± 0.182
2.8AspArg: 2.8 ± 0.228
4.304AspSer: 4.304 ± 0.367
4.464AspThr: 4.464 ± 0.316
4.8AspVal: 4.8 ± 0.272
1.216AspTrp: 1.216 ± 0.167
3.12AspTyr: 3.12 ± 0.243
0.0AspXaa: 0.0 ± 0.0
Glu
4.016GluAla: 4.016 ± 0.29
0.656GluCys: 0.656 ± 0.124
3.952GluAsp: 3.952 ± 0.313
4.816GluGlu: 4.816 ± 0.509
2.848GluPhe: 2.848 ± 0.23
4.384GluGly: 4.384 ± 0.336
1.216GluHis: 1.216 ± 0.195
4.48GluIle: 4.48 ± 0.302
3.456GluLys: 3.456 ± 0.345
4.704GluLeu: 4.704 ± 0.283
1.792GluMet: 1.792 ± 0.236
2.992GluAsn: 2.992 ± 0.245
1.824GluPro: 1.824 ± 0.177
2.224GluGln: 2.224 ± 0.197
2.56GluArg: 2.56 ± 0.217
4.688GluSer: 4.688 ± 0.411
4.224GluThr: 4.224 ± 0.208
4.688GluVal: 4.688 ± 0.274
0.96GluTrp: 0.96 ± 0.163
2.704GluTyr: 2.704 ± 0.225
0.0GluXaa: 0.0 ± 0.0
Phe
2.768PheAla: 2.768 ± 0.216
0.624PheCys: 0.624 ± 0.099
3.728PheAsp: 3.728 ± 0.253
2.768PheGlu: 2.768 ± 0.273
1.808PhePhe: 1.808 ± 0.155
3.36PheGly: 3.36 ± 0.452
0.688PheHis: 0.688 ± 0.118
2.384PheIle: 2.384 ± 0.232
2.528PheLys: 2.528 ± 0.217
2.848PheLeu: 2.848 ± 0.226
0.672PheMet: 0.672 ± 0.107
2.816PheAsn: 2.816 ± 0.272
1.712PhePro: 1.712 ± 0.139
1.392PheGln: 1.392 ± 0.169
1.792PheArg: 1.792 ± 0.155
3.344PheSer: 3.344 ± 0.363
3.456PheThr: 3.456 ± 0.384
3.392PheVal: 3.392 ± 0.269
0.4PheTrp: 0.4 ± 0.087
1.536PheTyr: 1.536 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
5.888GlyAla: 5.888 ± 0.466
0.896GlyCys: 0.896 ± 0.21
5.712GlyAsp: 5.712 ± 0.431
4.704GlyGlu: 4.704 ± 0.356
3.152GlyPhe: 3.152 ± 0.398
9.359GlyGly: 9.359 ± 1.138
1.024GlyHis: 1.024 ± 0.149
3.936GlyIle: 3.936 ± 0.262
3.872GlyLys: 3.872 ± 0.365
4.704GlyLeu: 4.704 ± 0.307
1.456GlyMet: 1.456 ± 0.184
4.352GlyAsn: 4.352 ± 0.338
2.256GlyPro: 2.256 ± 0.232
2.96GlyGln: 2.96 ± 0.231
3.184GlyArg: 3.184 ± 0.222
7.664GlySer: 7.664 ± 0.655
6.832GlyThr: 6.832 ± 0.538
5.536GlyVal: 5.536 ± 0.324
0.912GlyTrp: 0.912 ± 0.119
3.408GlyTyr: 3.408 ± 0.215
0.0GlyXaa: 0.0 ± 0.0
His
0.768HisAla: 0.768 ± 0.123
0.224HisCys: 0.224 ± 0.07
0.864HisAsp: 0.864 ± 0.124
0.944HisGlu: 0.944 ± 0.128
0.752HisPhe: 0.752 ± 0.132
1.056HisGly: 1.056 ± 0.153
0.304HisHis: 0.304 ± 0.073
0.8HisIle: 0.8 ± 0.12
1.072HisLys: 1.072 ± 0.167
1.008HisLeu: 1.008 ± 0.172
0.304HisMet: 0.304 ± 0.074
0.864HisAsn: 0.864 ± 0.129
0.752HisPro: 0.752 ± 0.122
0.576HisGln: 0.576 ± 0.104
0.608HisArg: 0.608 ± 0.107
0.72HisSer: 0.72 ± 0.113
1.088HisThr: 1.088 ± 0.167
0.72HisVal: 0.72 ± 0.135
0.272HisTrp: 0.272 ± 0.066
1.152HisTyr: 1.152 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
4.352IleAla: 4.352 ± 0.249
0.624IleCys: 0.624 ± 0.117
5.136IleAsp: 5.136 ± 0.32
4.384IleGlu: 4.384 ± 0.231
2.496IlePhe: 2.496 ± 0.202
4.672IleGly: 4.672 ± 0.347
0.8IleHis: 0.8 ± 0.134
3.92IleIle: 3.92 ± 0.34
3.936IleLys: 3.936 ± 0.294
4.4IleLeu: 4.4 ± 0.26
1.024IleMet: 1.024 ± 0.155
3.952IleAsn: 3.952 ± 0.287
3.072IlePro: 3.072 ± 0.254
2.4IleGln: 2.4 ± 0.236
2.608IleArg: 2.608 ± 0.209
4.272IleSer: 4.272 ± 0.367
5.376IleThr: 5.376 ± 0.417
3.84IleVal: 3.84 ± 0.299
0.608IleTrp: 0.608 ± 0.101
1.984IleTyr: 1.984 ± 0.178
0.0IleXaa: 0.0 ± 0.0
Lys
3.232LysAla: 3.232 ± 0.329
0.704LysCys: 0.704 ± 0.136
3.552LysAsp: 3.552 ± 0.332
4.144LysGlu: 4.144 ± 0.413
2.24LysPhe: 2.24 ± 0.187
3.04LysGly: 3.04 ± 0.275
0.864LysHis: 0.864 ± 0.141
3.712LysIle: 3.712 ± 0.288
4.416LysLys: 4.416 ± 0.536
4.256LysLeu: 4.256 ± 0.332
1.728LysMet: 1.728 ± 0.211
2.56LysAsn: 2.56 ± 0.243
1.968LysPro: 1.968 ± 0.228
2.304LysGln: 2.304 ± 0.268
2.144LysArg: 2.144 ± 0.187
3.216LysSer: 3.216 ± 0.361
3.808LysThr: 3.808 ± 0.291
3.984LysVal: 3.984 ± 0.334
0.544LysTrp: 0.544 ± 0.09
2.848LysTyr: 2.848 ± 0.266
0.0LysXaa: 0.0 ± 0.0
Leu
4.016LeuAla: 4.016 ± 0.231
0.656LeuCys: 0.656 ± 0.114
5.488LeuAsp: 5.488 ± 0.361
4.496LeuGlu: 4.496 ± 0.291
3.232LeuPhe: 3.232 ± 0.55
4.512LeuGly: 4.512 ± 0.268
1.136LeuHis: 1.136 ± 0.18
4.16LeuIle: 4.16 ± 0.274
4.544LeuLys: 4.544 ± 0.382
4.752LeuLeu: 4.752 ± 0.296
1.344LeuMet: 1.344 ± 0.176
4.304LeuAsn: 4.304 ± 0.257
3.008LeuPro: 3.008 ± 0.192
2.688LeuGln: 2.688 ± 0.231
3.088LeuArg: 3.088 ± 0.24
5.248LeuSer: 5.248 ± 0.251
5.552LeuThr: 5.552 ± 0.371
4.208LeuVal: 4.208 ± 0.253
0.592LeuTrp: 0.592 ± 0.119
3.024LeuTyr: 3.024 ± 0.244
0.0LeuXaa: 0.0 ± 0.0
Met
1.424MetAla: 1.424 ± 0.189
0.24MetCys: 0.24 ± 0.073
0.96MetAsp: 0.96 ± 0.156
1.152MetGlu: 1.152 ± 0.141
0.752MetPhe: 0.752 ± 0.137
1.008MetGly: 1.008 ± 0.15
0.288MetHis: 0.288 ± 0.076
1.44MetIle: 1.44 ± 0.164
1.44MetLys: 1.44 ± 0.214
1.52MetLeu: 1.52 ± 0.201
0.544MetMet: 0.544 ± 0.118
0.944MetAsn: 0.944 ± 0.132
0.96MetPro: 0.96 ± 0.141
0.688MetGln: 0.688 ± 0.112
0.864MetArg: 0.864 ± 0.105
1.552MetSer: 1.552 ± 0.168
1.52MetThr: 1.52 ± 0.208
1.024MetVal: 1.024 ± 0.141
0.176MetTrp: 0.176 ± 0.054
0.752MetTyr: 0.752 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.6AsnAla: 3.6 ± 0.295
0.48AsnCys: 0.48 ± 0.126
3.536AsnAsp: 3.536 ± 0.227
2.784AsnGlu: 2.784 ± 0.22
2.832AsnPhe: 2.832 ± 0.271
4.56AsnGly: 4.56 ± 0.324
0.912AsnHis: 0.912 ± 0.117
3.936AsnIle: 3.936 ± 0.256
2.704AsnLys: 2.704 ± 0.226
3.904AsnLeu: 3.904 ± 0.312
0.832AsnMet: 0.832 ± 0.123
3.12AsnAsn: 3.12 ± 0.214
2.896AsnPro: 2.896 ± 0.303
1.872AsnGln: 1.872 ± 0.176
2.048AsnArg: 2.048 ± 0.196
3.872AsnSer: 3.872 ± 0.33
3.888AsnThr: 3.888 ± 0.317
3.952AsnVal: 3.952 ± 0.295
0.704AsnTrp: 0.704 ± 0.1
2.688AsnTyr: 2.688 ± 0.244
0.0AsnXaa: 0.0 ± 0.0
Pro
3.088ProAla: 3.088 ± 0.233
0.144ProCys: 0.144 ± 0.053
3.216ProAsp: 3.216 ± 0.269
3.28ProGlu: 3.28 ± 0.275
1.648ProPhe: 1.648 ± 0.174
3.68ProGly: 3.68 ± 0.329
0.512ProHis: 0.512 ± 0.103
2.288ProIle: 2.288 ± 0.264
2.016ProLys: 2.016 ± 0.226
2.928ProLeu: 2.928 ± 0.277
0.496ProMet: 0.496 ± 0.109
2.24ProAsn: 2.24 ± 0.206
1.84ProPro: 1.84 ± 0.217
1.392ProGln: 1.392 ± 0.196
1.424ProArg: 1.424 ± 0.144
2.8ProSer: 2.8 ± 0.26
3.088ProThr: 3.088 ± 0.224
2.96ProVal: 2.96 ± 0.209
0.512ProTrp: 0.512 ± 0.093
1.712ProTyr: 1.712 ± 0.194
0.0ProXaa: 0.0 ± 0.0
Gln
2.256GlnAla: 2.256 ± 0.223
0.288GlnCys: 0.288 ± 0.062
2.16GlnAsp: 2.16 ± 0.173
2.448GlnGlu: 2.448 ± 0.22
1.504GlnPhe: 1.504 ± 0.16
2.32GlnGly: 2.32 ± 0.189
0.528GlnHis: 0.528 ± 0.114
2.896GlnIle: 2.896 ± 0.202
2.208GlnLys: 2.208 ± 0.251
2.72GlnLeu: 2.72 ± 0.201
0.752GlnMet: 0.752 ± 0.124
1.792GlnAsn: 1.792 ± 0.192
1.632GlnPro: 1.632 ± 0.154
1.488GlnGln: 1.488 ± 0.164
1.408GlnArg: 1.408 ± 0.169
2.656GlnSer: 2.656 ± 0.171
2.208GlnThr: 2.208 ± 0.197
2.176GlnVal: 2.176 ± 0.199
0.4GlnTrp: 0.4 ± 0.071
1.808GlnTyr: 1.808 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
2.72ArgAla: 2.72 ± 0.236
0.256ArgCys: 0.256 ± 0.061
2.464ArgAsp: 2.464 ± 0.213
2.288ArgGlu: 2.288 ± 0.244
2.16ArgPhe: 2.16 ± 0.171
3.168ArgGly: 3.168 ± 0.205
0.656ArgHis: 0.656 ± 0.122
2.832ArgIle: 2.832 ± 0.193
2.544ArgLys: 2.544 ± 0.251
3.136ArgLeu: 3.136 ± 0.21
0.816ArgMet: 0.816 ± 0.109
2.0ArgAsn: 2.0 ± 0.196
1.504ArgPro: 1.504 ± 0.172
1.392ArgGln: 1.392 ± 0.155
2.048ArgArg: 2.048 ± 0.201
2.416ArgSer: 2.416 ± 0.207
2.144ArgThr: 2.144 ± 0.254
2.464ArgVal: 2.464 ± 0.231
0.48ArgTrp: 0.48 ± 0.089
2.272ArgTyr: 2.272 ± 0.171
0.0ArgXaa: 0.0 ± 0.0
Ser
4.272SerAla: 4.272 ± 0.354
0.64SerCys: 0.64 ± 0.125
4.416SerAsp: 4.416 ± 0.232
3.92SerGlu: 3.92 ± 0.252
3.472SerPhe: 3.472 ± 0.311
8.271SerGly: 8.271 ± 0.74
0.88SerHis: 0.88 ± 0.124
4.816SerIle: 4.816 ± 0.301
3.696SerLys: 3.696 ± 0.302
5.072SerLeu: 5.072 ± 0.277
1.344SerMet: 1.344 ± 0.166
4.336SerAsn: 4.336 ± 0.359
2.88SerPro: 2.88 ± 0.262
2.624SerGln: 2.624 ± 0.204
2.272SerArg: 2.272 ± 0.175
6.0SerSer: 6.0 ± 0.627
5.328SerThr: 5.328 ± 0.398
5.104SerVal: 5.104 ± 0.281
0.624SerTrp: 0.624 ± 0.1
3.328SerTyr: 3.328 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
5.216ThrAla: 5.216 ± 0.411
0.56ThrCys: 0.56 ± 0.128
4.288ThrAsp: 4.288 ± 0.34
4.048ThrGlu: 4.048 ± 0.285
3.632ThrPhe: 3.632 ± 0.282
7.184ThrGly: 7.184 ± 0.491
0.896ThrHis: 0.896 ± 0.141
5.344ThrIle: 5.344 ± 0.395
3.104ThrLys: 3.104 ± 0.234
5.872ThrLeu: 5.872 ± 0.421
0.992ThrMet: 0.992 ± 0.1
3.936ThrAsn: 3.936 ± 0.357
3.6ThrPro: 3.6 ± 0.271
2.544ThrGln: 2.544 ± 0.212
2.624ThrArg: 2.624 ± 0.28
4.896ThrSer: 4.896 ± 0.415
5.856ThrThr: 5.856 ± 0.455
5.84ThrVal: 5.84 ± 0.313
0.784ThrTrp: 0.784 ± 0.142
3.104ThrTyr: 3.104 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
4.688ValAla: 4.688 ± 0.384
0.496ValCys: 0.496 ± 0.09
5.312ValAsp: 5.312 ± 0.351
4.448ValGlu: 4.448 ± 0.263
2.736ValPhe: 2.736 ± 0.23
4.64ValGly: 4.64 ± 0.334
1.008ValHis: 1.008 ± 0.143
4.048ValIle: 4.048 ± 0.234
3.648ValLys: 3.648 ± 0.326
4.256ValLeu: 4.256 ± 0.268
1.264ValMet: 1.264 ± 0.177
4.08ValAsn: 4.08 ± 0.307
3.136ValPro: 3.136 ± 0.242
1.968ValGln: 1.968 ± 0.16
3.12ValArg: 3.12 ± 0.231
5.6ValSer: 5.6 ± 0.364
5.696ValThr: 5.696 ± 0.344
4.784ValVal: 4.784 ± 0.324
0.544ValTrp: 0.544 ± 0.103
2.96ValTyr: 2.96 ± 0.237
0.0ValXaa: 0.0 ± 0.0
Trp
0.64TrpAla: 0.64 ± 0.1
0.208TrpCys: 0.208 ± 0.063
0.64TrpAsp: 0.64 ± 0.122
0.704TrpGlu: 0.704 ± 0.132
0.416TrpPhe: 0.416 ± 0.084
0.736TrpGly: 0.736 ± 0.106
0.352TrpHis: 0.352 ± 0.083
0.592TrpIle: 0.592 ± 0.093
0.688TrpLys: 0.688 ± 0.104
0.688TrpLeu: 0.688 ± 0.104
0.272TrpMet: 0.272 ± 0.072
0.816TrpAsn: 0.816 ± 0.112
0.192TrpPro: 0.192 ± 0.055
0.464TrpGln: 0.464 ± 0.079
0.496TrpArg: 0.496 ± 0.104
0.784TrpSer: 0.784 ± 0.119
0.752TrpThr: 0.752 ± 0.109
0.64TrpVal: 0.64 ± 0.097
0.144TrpTrp: 0.144 ± 0.055
0.592TrpTyr: 0.592 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.4TyrAla: 2.4 ± 0.172
0.448TyrCys: 0.448 ± 0.108
3.088TyrAsp: 3.088 ± 0.214
2.768TyrGlu: 2.768 ± 0.263
1.936TyrPhe: 1.936 ± 0.206
3.104TyrGly: 3.104 ± 0.29
0.64TyrHis: 0.64 ± 0.103
3.12TyrIle: 3.12 ± 0.229
2.144TyrLys: 2.144 ± 0.236
3.28TyrLeu: 3.28 ± 0.292
0.72TyrMet: 0.72 ± 0.141
2.752TyrAsn: 2.752 ± 0.212
1.872TyrPro: 1.872 ± 0.186
1.888TyrGln: 1.888 ± 0.217
2.032TyrArg: 2.032 ± 0.218
3.072TyrSer: 3.072 ± 0.216
3.152TyrThr: 3.152 ± 0.227
3.232TyrVal: 3.232 ± 0.258
0.352TyrTrp: 0.352 ± 0.072
2.368TyrTyr: 2.368 ± 0.287
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 240 proteins (62505 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski