Amino acid dipepetide frequency for bacterium 0.1xD8-71

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.25AlaAla: 7.25 ± 0.097	1.168AlaCys: 1.168 ± 0.029	4.811AlaAsp: 4.811 ± 0.069	5.287AlaGlu: 5.287 ± 0.076	3.072AlaPhe: 3.072 ± 0.057	6.551AlaGly: 6.551 ± 0.084	1.071AlaHis: 1.071 ± 0.03	4.441AlaIle: 4.441 ± 0.074	4.118AlaLys: 4.118 ± 0.058	6.845AlaLeu: 6.845 ± 0.081	2.265AlaMet: 2.265 ± 0.044	2.366AlaAsn: 2.366 ± 0.054	1.957AlaPro: 1.957 ± 0.049	2.664AlaGln: 2.664 ± 0.057	3.429AlaArg: 3.429 ± 0.054	3.692AlaSer: 3.692 ± 0.066	2.862AlaThr: 2.862 ± 0.059	6.716AlaVal: 6.716 ± 0.088	0.697AlaTrp: 0.697 ± 0.027	3.29AlaTyr: 3.29 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	1.05CysAla: 1.05 ± 0.032	0.262CysCys: 0.262 ± 0.015	0.852CysAsp: 0.852 ± 0.027	0.973CysGlu: 0.973 ± 0.026	0.72CysPhe: 0.72 ± 0.028	1.5CysGly: 1.5 ± 0.037	0.317CysHis: 0.317 ± 0.015	1.082CysIle: 1.082 ± 0.032	0.888CysLys: 0.888 ± 0.024	1.42CysLeu: 1.42 ± 0.036	0.524CysMet: 0.524 ± 0.02	0.599CysAsn: 0.599 ± 0.023	0.587CysPro: 0.587 ± 0.022	0.445CysGln: 0.445 ± 0.019	0.801CysArg: 0.801 ± 0.027	0.857CysSer: 0.857 ± 0.03	0.705CysThr: 0.705 ± 0.027	1.102CysVal: 1.102 ± 0.032	0.129CysTrp: 0.129 ± 0.009	0.685CysTyr: 0.685 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.972AspAla: 3.972 ± 0.079	0.818AspCys: 0.818 ± 0.028	2.945AspAsp: 2.945 ± 0.062	4.482AspGlu: 4.482 ± 0.065	2.554AspPhe: 2.554 ± 0.047	4.429AspGly: 4.429 ± 0.07	0.89AspHis: 0.89 ± 0.034	4.679AspIle: 4.679 ± 0.06	3.73AspLys: 3.73 ± 0.065	4.456AspLeu: 4.456 ± 0.062	2.171AspMet: 2.171 ± 0.039	2.361AspAsn: 2.361 ± 0.05	1.598AspPro: 1.598 ± 0.041	1.326AspGln: 1.326 ± 0.035	2.805AspArg: 2.805 ± 0.047	3.141AspSer: 3.141 ± 0.053	3.197AspThr: 3.197 ± 0.05	3.821AspVal: 3.821 ± 0.059	0.682AspTrp: 0.682 ± 0.027	3.08AspTyr: 3.08 ± 0.061	0.0AspXaa: 0.0 ± 0.0
Glu	5.991GluAla: 5.991 ± 0.091	0.833GluCys: 0.833 ± 0.025	4.59GluAsp: 4.59 ± 0.065	8.409GluGlu: 8.409 ± 0.107	2.483GluPhe: 2.483 ± 0.044	4.766GluGly: 4.766 ± 0.076	1.402GluHis: 1.402 ± 0.04	5.614GluIle: 5.614 ± 0.078	6.284GluLys: 6.284 ± 0.073	6.51GluLeu: 6.51 ± 0.076	2.513GluMet: 2.513 ± 0.05	3.925GluAsn: 3.925 ± 0.052	1.881GluPro: 1.881 ± 0.044	3.475GluGln: 3.475 ± 0.053	3.885GluArg: 3.885 ± 0.079	3.542GluSer: 3.542 ± 0.062	3.841GluThr: 3.841 ± 0.062	4.522GluVal: 4.522 ± 0.064	0.764GluTrp: 0.764 ± 0.027	3.466GluTyr: 3.466 ± 0.061	0.0GluXaa: 0.0 ± 0.0
Phe	3.026PheAla: 3.026 ± 0.057	0.806PheCys: 0.806 ± 0.025	2.476PheAsp: 2.476 ± 0.049	2.484PheGlu: 2.484 ± 0.047	1.877PhePhe: 1.877 ± 0.049	2.865PheGly: 2.865 ± 0.055	0.892PheHis: 0.892 ± 0.025	2.68PheIle: 2.68 ± 0.061	1.784PheLys: 1.784 ± 0.041	4.08PheLeu: 4.08 ± 0.078	1.215PheMet: 1.215 ± 0.038	1.348PheAsn: 1.348 ± 0.039	1.322PhePro: 1.322 ± 0.034	1.347PheGln: 1.347 ± 0.034	1.864PheArg: 1.864 ± 0.037	2.588PheSer: 2.588 ± 0.054	2.205PheThr: 2.205 ± 0.044	2.911PheVal: 2.911 ± 0.06	0.498PheTrp: 0.498 ± 0.023	1.983PheTyr: 1.983 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	4.801GlyAla: 4.801 ± 0.074	1.279GlyCys: 1.279 ± 0.037	3.517GlyAsp: 3.517 ± 0.067	5.024GlyGlu: 5.024 ± 0.075	3.073GlyPhe: 3.073 ± 0.059	4.754GlyGly: 4.754 ± 0.087	1.209GlyHis: 1.209 ± 0.031	6.165GlyIle: 6.165 ± 0.077	4.836GlyLys: 4.836 ± 0.064	5.741GlyLeu: 5.741 ± 0.069	2.613GlyMet: 2.613 ± 0.05	3.153GlyAsn: 3.153 ± 0.061	1.196GlyPro: 1.196 ± 0.031	2.471GlyGln: 2.471 ± 0.05	3.853GlyArg: 3.853 ± 0.072	4.05GlySer: 4.05 ± 0.079	3.961GlyThr: 3.961 ± 0.07	4.916GlyVal: 4.916 ± 0.069	0.694GlyTrp: 0.694 ± 0.025	3.497GlyTyr: 3.497 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	1.11HisAla: 1.11 ± 0.031	0.32HisCys: 0.32 ± 0.018	0.943HisAsp: 0.943 ± 0.028	1.116HisGlu: 1.116 ± 0.032	0.832HisPhe: 0.832 ± 0.028	1.27HisGly: 1.27 ± 0.031	0.418HisHis: 0.418 ± 0.022	1.411HisIle: 1.411 ± 0.037	1.115HisLys: 1.115 ± 0.03	1.547HisLeu: 1.547 ± 0.039	0.55HisMet: 0.55 ± 0.023	0.787HisAsn: 0.787 ± 0.029	0.794HisPro: 0.794 ± 0.026	0.508HisGln: 0.508 ± 0.019	0.841HisArg: 0.841 ± 0.031	0.877HisSer: 0.877 ± 0.026	0.953HisThr: 0.953 ± 0.033	1.146HisVal: 1.146 ± 0.034	0.185HisTrp: 0.185 ± 0.013	0.874HisTyr: 0.874 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.5IleAla: 5.5 ± 0.071	1.374IleCys: 1.374 ± 0.032	3.983IleAsp: 3.983 ± 0.064	4.851IleGlu: 4.851 ± 0.069	2.775IlePhe: 2.775 ± 0.052	4.836IleGly: 4.836 ± 0.073	1.34IleHis: 1.34 ± 0.033	4.845IleIle: 4.845 ± 0.071	3.761IleLys: 3.761 ± 0.068	7.216IleLeu: 7.216 ± 0.099	2.104IleMet: 2.104 ± 0.041	2.836IleAsn: 2.836 ± 0.049	2.992IlePro: 2.992 ± 0.055	2.285IleGln: 2.285 ± 0.04	3.908IleArg: 3.908 ± 0.06	4.614IleSer: 4.614 ± 0.072	3.992IleThr: 3.992 ± 0.062	4.972IleVal: 4.972 ± 0.071	0.706IleTrp: 0.706 ± 0.027	3.063IleTyr: 3.063 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.568LysAla: 4.568 ± 0.067	0.706LysCys: 0.706 ± 0.024	3.758LysAsp: 3.758 ± 0.065	6.3LysGlu: 6.3 ± 0.082	1.713LysPhe: 1.713 ± 0.038	3.873LysGly: 3.873 ± 0.059	0.92LysHis: 0.92 ± 0.028	4.545LysIle: 4.545 ± 0.071	5.034LysLys: 5.034 ± 0.083	4.854LysLeu: 4.854 ± 0.069	2.065LysMet: 2.065 ± 0.047	3.042LysAsn: 3.042 ± 0.048	1.836LysPro: 1.836 ± 0.041	2.334LysGln: 2.334 ± 0.044	2.995LysArg: 2.995 ± 0.052	3.071LysSer: 3.071 ± 0.059	3.202LysThr: 3.202 ± 0.049	3.943LysVal: 3.943 ± 0.066	0.65LysTrp: 0.65 ± 0.025	2.64LysTyr: 2.64 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.852LeuAla: 6.852 ± 0.083	1.658LeuCys: 1.658 ± 0.044	4.857LeuAsp: 4.857 ± 0.068	6.251LeuGlu: 6.251 ± 0.077	3.968LeuPhe: 3.968 ± 0.07	5.69LeuGly: 5.69 ± 0.085	1.763LeuHis: 1.763 ± 0.036	5.659LeuIle: 5.659 ± 0.076	5.32LeuLys: 5.32 ± 0.076	9.232LeuLeu: 9.232 ± 0.135	2.759LeuMet: 2.759 ± 0.051	3.48LeuAsn: 3.48 ± 0.065	3.4LeuPro: 3.4 ± 0.06	3.31LeuGln: 3.31 ± 0.057	3.95LeuArg: 3.95 ± 0.063	6.245LeuSer: 6.245 ± 0.081	4.974LeuThr: 4.974 ± 0.066	5.659LeuVal: 5.659 ± 0.072	0.931LeuTrp: 0.931 ± 0.032	3.86LeuTyr: 3.86 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	2.592MetAla: 2.592 ± 0.047	0.392MetCys: 0.392 ± 0.017	2.019MetAsp: 2.019 ± 0.046	3.074MetGlu: 3.074 ± 0.049	1.017MetPhe: 1.017 ± 0.035	2.243MetGly: 2.243 ± 0.045	0.478MetHis: 0.478 ± 0.018	2.235MetIle: 2.235 ± 0.041	2.366MetLys: 2.366 ± 0.039	2.758MetLeu: 2.758 ± 0.053	1.1MetMet: 1.1 ± 0.032	1.521MetAsn: 1.521 ± 0.04	1.217MetPro: 1.217 ± 0.031	1.26MetGln: 1.26 ± 0.031	1.455MetArg: 1.455 ± 0.036	1.806MetSer: 1.806 ± 0.042	1.891MetThr: 1.891 ± 0.044	2.102MetVal: 2.102 ± 0.042	0.254MetTrp: 0.254 ± 0.015	0.961MetTyr: 0.961 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.841AsnAla: 2.841 ± 0.05	0.632AsnCys: 0.632 ± 0.023	2.127AsnAsp: 2.127 ± 0.042	2.686AsnGlu: 2.686 ± 0.057	1.429AsnPhe: 1.429 ± 0.037	3.391AsnGly: 3.391 ± 0.061	0.798AsnHis: 0.798 ± 0.025	3.22AsnIle: 3.22 ± 0.064	2.335AsnLys: 2.335 ± 0.047	3.71AsnLeu: 3.71 ± 0.063	1.456AsnMet: 1.456 ± 0.039	1.775AsnAsn: 1.775 ± 0.044	1.87AsnPro: 1.87 ± 0.037	1.41AsnGln: 1.41 ± 0.036	2.226AsnArg: 2.226 ± 0.048	2.207AsnSer: 2.207 ± 0.045	2.285AsnThr: 2.285 ± 0.051	2.823AsnVal: 2.823 ± 0.056	0.456AsnTrp: 0.456 ± 0.02	1.797AsnTyr: 1.797 ± 0.043	0.0AsnXaa: 0.0 ± 0.0
Pro	2.559ProAla: 2.559 ± 0.051	0.451ProCys: 0.451 ± 0.02	2.308ProAsp: 2.308 ± 0.048	3.061ProGlu: 3.061 ± 0.053	1.475ProPhe: 1.475 ± 0.03	2.263ProGly: 2.263 ± 0.049	0.544ProHis: 0.544 ± 0.024	1.88ProIle: 1.88 ± 0.041	1.592ProLys: 1.592 ± 0.038	2.636ProLeu: 2.636 ± 0.056	0.929ProMet: 0.929 ± 0.027	1.1ProAsn: 1.1 ± 0.033	0.712ProPro: 0.712 ± 0.027	1.121ProGln: 1.121 ± 0.033	1.065ProArg: 1.065 ± 0.037	1.523ProSer: 1.523 ± 0.035	1.392ProThr: 1.392 ± 0.033	3.008ProVal: 3.008 ± 0.054	0.295ProTrp: 0.295 ± 0.015	1.548ProTyr: 1.548 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.773GlnAla: 2.773 ± 0.053	0.396GlnCys: 0.396 ± 0.021	1.901GlnAsp: 1.901 ± 0.039	3.31GlnGlu: 3.31 ± 0.056	1.207GlnPhe: 1.207 ± 0.031	2.257GlnGly: 2.257 ± 0.045	0.452GlnHis: 0.452 ± 0.02	2.963GlnIle: 2.963 ± 0.057	2.746GlnLys: 2.746 ± 0.052	2.725GlnLeu: 2.725 ± 0.051	1.432GlnMet: 1.432 ± 0.036	1.748GlnAsn: 1.748 ± 0.044	0.93GlnPro: 0.93 ± 0.027	1.316GlnGln: 1.316 ± 0.036	1.497GlnArg: 1.497 ± 0.038	1.78GlnSer: 1.78 ± 0.039	1.909GlnThr: 1.909 ± 0.051	2.184GlnVal: 2.184 ± 0.039	0.367GlnTrp: 0.367 ± 0.017	1.621GlnTyr: 1.621 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.934ArgAla: 2.934 ± 0.053	0.625ArgCys: 0.625 ± 0.023	2.447ArgAsp: 2.447 ± 0.046	4.467ArgGlu: 4.467 ± 0.072	1.926ArgPhe: 1.926 ± 0.042	2.679ArgGly: 2.679 ± 0.052	0.894ArgHis: 0.894 ± 0.03	4.051ArgIle: 4.051 ± 0.063	3.787ArgLys: 3.787 ± 0.056	4.218ArgLeu: 4.218 ± 0.064	1.805ArgMet: 1.805 ± 0.044	2.234ArgAsn: 2.234 ± 0.044	1.334ArgPro: 1.334 ± 0.036	2.297ArgGln: 2.297 ± 0.045	2.816ArgArg: 2.816 ± 0.058	2.124ArgSer: 2.124 ± 0.045	2.426ArgThr: 2.426 ± 0.049	2.933ArgVal: 2.933 ± 0.048	0.473ArgTrp: 0.473 ± 0.023	2.143ArgTyr: 2.143 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.137SerAla: 4.137 ± 0.062	0.798SerCys: 0.798 ± 0.025	3.122SerAsp: 3.122 ± 0.054	3.665SerGlu: 3.665 ± 0.052	2.584SerPhe: 2.584 ± 0.049	4.707SerGly: 4.707 ± 0.077	1.038SerHis: 1.038 ± 0.029	3.922SerIle: 3.922 ± 0.059	2.795SerLys: 2.795 ± 0.054	5.122SerLeu: 5.122 ± 0.068	1.853SerMet: 1.853 ± 0.043	1.998SerAsn: 1.998 ± 0.048	1.632SerPro: 1.632 ± 0.04	1.808SerGln: 1.808 ± 0.04	2.858SerArg: 2.858 ± 0.048	3.089SerSer: 3.089 ± 0.068	2.625SerThr: 2.625 ± 0.054	4.377SerVal: 4.377 ± 0.059	0.509SerTrp: 0.509 ± 0.021	2.662SerTyr: 2.662 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	4.349ThrAla: 4.349 ± 0.073	0.66ThrCys: 0.66 ± 0.022	3.22ThrAsp: 3.22 ± 0.055	3.809ThrGlu: 3.809 ± 0.065	2.085ThrPhe: 2.085 ± 0.047	4.608ThrGly: 4.608 ± 0.073	0.808ThrHis: 0.808 ± 0.026	3.668ThrIle: 3.668 ± 0.067	2.61ThrLys: 2.61 ± 0.048	4.714ThrLeu: 4.714 ± 0.068	1.457ThrMet: 1.457 ± 0.035	1.845ThrAsn: 1.845 ± 0.042	1.923ThrPro: 1.923 ± 0.041	1.729ThrGln: 1.729 ± 0.043	2.147ThrArg: 2.147 ± 0.042	2.562ThrSer: 2.562 ± 0.051	2.533ThrThr: 2.533 ± 0.063	4.328ThrVal: 4.328 ± 0.063	0.465ThrTrp: 0.465 ± 0.019	2.173ThrTyr: 2.173 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	4.672ValAla: 4.672 ± 0.067	1.39ValCys: 1.39 ± 0.038	3.839ValAsp: 3.839 ± 0.058	5.079ValGlu: 5.079 ± 0.064	3.091ValPhe: 3.091 ± 0.056	4.19ValGly: 4.19 ± 0.069	1.09ValHis: 1.09 ± 0.031	5.157ValIle: 5.157 ± 0.072	4.079ValLys: 4.079 ± 0.063	6.667ValLeu: 6.667 ± 0.082	2.325ValMet: 2.325 ± 0.044	2.911ValAsn: 2.911 ± 0.049	2.502ValPro: 2.502 ± 0.046	2.061ValGln: 2.061 ± 0.042	3.301ValArg: 3.301 ± 0.061	4.717ValSer: 4.717 ± 0.062	4.055ValThr: 4.055 ± 0.061	4.937ValVal: 4.937 ± 0.074	0.758ValTrp: 0.758 ± 0.026	3.002ValTyr: 3.002 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.589TrpAla: 0.589 ± 0.021	0.184TrpCys: 0.184 ± 0.012	0.605TrpAsp: 0.605 ± 0.022	0.825TrpGlu: 0.825 ± 0.027	0.448TrpPhe: 0.448 ± 0.022	0.726TrpGly: 0.726 ± 0.027	0.234TrpHis: 0.234 ± 0.016	0.729TrpIle: 0.729 ± 0.026	0.715TrpLys: 0.715 ± 0.025	0.962TrpLeu: 0.962 ± 0.028	0.345TrpMet: 0.345 ± 0.017	0.569TrpAsn: 0.569 ± 0.024	0.215TrpPro: 0.215 ± 0.013	0.492TrpGln: 0.492 ± 0.021	0.455TrpArg: 0.455 ± 0.02	0.468TrpSer: 0.468 ± 0.02	0.42TrpThr: 0.42 ± 0.02	0.55TrpVal: 0.55 ± 0.023	0.133TrpTrp: 0.133 ± 0.01	0.42TrpTyr: 0.42 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.99TyrAla: 2.99 ± 0.047	0.747TyrCys: 0.747 ± 0.022	2.82TyrAsp: 2.82 ± 0.069	3.543TyrGlu: 3.543 ± 0.061	1.922TyrPhe: 1.922 ± 0.043	3.206TyrGly: 3.206 ± 0.052	1.037TyrHis: 1.037 ± 0.03	3.005TyrIle: 3.005 ± 0.062	2.154TyrLys: 2.154 ± 0.043	4.404TyrLeu: 4.404 ± 0.061	1.261TyrMet: 1.261 ± 0.037	1.874TyrAsn: 1.874 ± 0.039	1.564TyrPro: 1.564 ± 0.038	1.857TyrGln: 1.857 ± 0.045	2.461TyrArg: 2.461 ± 0.045	2.348TyrSer: 2.348 ± 0.053	2.268TyrThr: 2.268 ± 0.052	2.87TyrVal: 2.87 ± 0.057	0.436TyrTrp: 0.436 ± 0.02	2.166TyrTyr: 2.166 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3809 proteins (1197674 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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