endosymbiont of Rhynchophorus ferrugineus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Nardonella; Candidatus Nardonella dryophthoridicola

Average proteome isoelectric point is 8.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5TGQ3|A0A2Z5TGQ3_9GAMM RNA polymerase sigma factor RpoD OS=endosymbiont of Rhynchophorus ferrugineus OX=1972133 GN=rpoD PE=3 SV=1
MM1 pKa = 7.14TSKK4 pKa = 10.27EE5 pKa = 4.11VKK7 pKa = 9.67FGNDD11 pKa = 2.87ARR13 pKa = 11.84IKK15 pKa = 9.5MLNGVNILANAVKK28 pKa = 9.39VTLGPKK34 pKa = 9.31GRR36 pKa = 11.84NVVLDD41 pKa = 3.8KK42 pKa = 11.57SFGSPLITKK51 pKa = 10.1DD52 pKa = 3.33GVSVARR58 pKa = 11.84EE59 pKa = 3.58IEE61 pKa = 4.19LEE63 pKa = 4.15DD64 pKa = 3.41KK65 pKa = 10.65FEE67 pKa = 4.45NMGAQMVKK75 pKa = 10.17EE76 pKa = 4.36VASKK80 pKa = 10.68VNDD83 pKa = 3.5MAGDD87 pKa = 3.9GTTTATLLAQSIINEE102 pKa = 4.1GLKK105 pKa = 10.74AIAAGMNPMDD115 pKa = 4.49IKK117 pKa = 10.86RR118 pKa = 11.84GIDD121 pKa = 3.12KK122 pKa = 10.73AVNYY126 pKa = 9.93AINEE130 pKa = 4.18LKK132 pKa = 10.34RR133 pKa = 11.84ISVSCSDD140 pKa = 3.91YY141 pKa = 11.27KK142 pKa = 11.27SIIQVGTISANSDD155 pKa = 3.24EE156 pKa = 5.27NIGKK160 pKa = 10.02LIADD164 pKa = 3.67AMEE167 pKa = 4.29KK168 pKa = 10.17VGKK171 pKa = 10.01EE172 pKa = 3.68GVITVEE178 pKa = 4.37EE179 pKa = 4.61GSGLNDD185 pKa = 3.08EE186 pKa = 4.78LEE188 pKa = 4.45IVEE191 pKa = 5.13GMQFDD196 pKa = 3.76RR197 pKa = 11.84GYY199 pKa = 10.76LSPYY203 pKa = 9.83FINKK207 pKa = 8.68PDD209 pKa = 3.91FNIVEE214 pKa = 4.29FDD216 pKa = 3.3NPYY219 pKa = 10.62ILLIDD224 pKa = 3.9KK225 pKa = 10.17KK226 pKa = 9.28ITGIRR231 pKa = 11.84EE232 pKa = 3.92LLPILEE238 pKa = 5.49NITKK242 pKa = 10.03SNKK245 pKa = 9.22PILIIAEE252 pKa = 4.62DD253 pKa = 3.71IEE255 pKa = 4.92GEE257 pKa = 4.08ALATLVVNNMRR268 pKa = 11.84GIFRR272 pKa = 11.84AVAVKK277 pKa = 10.65APGFGDD283 pKa = 3.21RR284 pKa = 11.84RR285 pKa = 11.84KK286 pKa = 11.2SMLQDD291 pKa = 3.1IAILTGGILISEE303 pKa = 4.69EE304 pKa = 3.71IGLDD308 pKa = 3.24ISKK311 pKa = 7.65TTLKK315 pKa = 10.97DD316 pKa = 3.03LGNARR321 pKa = 11.84KK322 pKa = 9.85IIVDD326 pKa = 3.76KK327 pKa = 11.43DD328 pKa = 3.1NTTIIDD334 pKa = 4.02GYY336 pKa = 10.82GDD338 pKa = 3.63KK339 pKa = 10.93KK340 pKa = 10.99CINQRR345 pKa = 11.84INQINKK351 pKa = 9.46EE352 pKa = 3.96KK353 pKa = 10.38EE354 pKa = 4.17QTDD357 pKa = 3.71SEE359 pKa = 4.34YY360 pKa = 11.24DD361 pKa = 3.37KK362 pKa = 11.5EE363 pKa = 4.12KK364 pKa = 10.11LQEE367 pKa = 4.59RR368 pKa = 11.84IAKK371 pKa = 10.18LSGGVALIRR380 pKa = 11.84VGAATEE386 pKa = 4.09VEE388 pKa = 4.27MKK390 pKa = 9.93EE391 pKa = 3.98KK392 pKa = 10.45KK393 pKa = 10.29SRR395 pKa = 11.84VEE397 pKa = 3.85DD398 pKa = 3.56ALNATRR404 pKa = 11.84AAVEE408 pKa = 4.16EE409 pKa = 4.64GVVAGGGVALVRR421 pKa = 11.84VSKK424 pKa = 11.01NLLNLKK430 pKa = 9.8GDD432 pKa = 3.98NEE434 pKa = 4.23DD435 pKa = 3.3QNVGIKK441 pKa = 10.09VAIKK445 pKa = 10.69SMEE448 pKa = 4.05SPLRR452 pKa = 11.84QIVSNAGEE460 pKa = 4.19EE461 pKa = 3.94PSIILNKK468 pKa = 10.1IKK470 pKa = 10.79LSDD473 pKa = 3.59GNFGYY478 pKa = 10.88NAFTEE483 pKa = 4.95EE484 pKa = 4.31YY485 pKa = 10.8GDD487 pKa = 4.04MIKK490 pKa = 9.78MGILDD495 pKa = 3.78PTKK498 pKa = 8.92VTRR501 pKa = 11.84SALQYY506 pKa = 8.67AASVAGLMITTEE518 pKa = 4.08CMITEE523 pKa = 4.32IKK525 pKa = 10.53KK526 pKa = 10.8EE527 pKa = 3.71EE528 pKa = 4.42DD529 pKa = 3.82KK530 pKa = 11.69NFDD533 pKa = 3.67TQNPNMNNMNNIMM546 pKa = 4.24

Molecular weight: 59.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5T9C2|A0A2Z5T9C2_9GAMM UDP-N-acetylenolpyruvoylglucosamine reductase OS=endosymbiont of Rhynchophorus ferrugineus OX=1972133 GN=murB PE=3 SV=1
MM1 pKa = 7.57SRR3 pKa = 11.84GHH5 pKa = 6.04YY6 pKa = 10.48KK7 pKa = 9.6MLFKK11 pKa = 10.75NLCNSLIKK19 pKa = 10.42YY20 pKa = 7.98EE21 pKa = 4.45KK22 pKa = 10.09IKK24 pKa = 8.54TTIKK28 pKa = 9.77RR29 pKa = 11.84AKK31 pKa = 8.86EE32 pKa = 3.44IKK34 pKa = 10.25KK35 pKa = 10.08IIEE38 pKa = 4.41PIINRR43 pKa = 11.84SKK45 pKa = 11.55VNILHH50 pKa = 6.58NKK52 pKa = 8.9RR53 pKa = 11.84IVYY56 pKa = 10.21SYY58 pKa = 11.34LNNRR62 pKa = 11.84NNVYY66 pKa = 10.93KK67 pKa = 10.73LFNDD71 pKa = 2.87IGYY74 pKa = 9.94RR75 pKa = 11.84YY76 pKa = 9.96KK77 pKa = 10.87NRR79 pKa = 11.84NGGYY83 pKa = 7.48TRR85 pKa = 11.84INKK88 pKa = 9.81LYY90 pKa = 9.2YY91 pKa = 10.15RR92 pKa = 11.84KK93 pKa = 9.95GDD95 pKa = 3.2SSLIVCISLVV105 pKa = 3.26

Molecular weight: 12.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

196

0

196

62158

37

1338

317.1

37.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.369 ± 0.115

1.03 ± 0.047

3.94 ± 0.122

4.056 ± 0.144

5.64 ± 0.189

3.758 ± 0.182

0.981 ± 0.043

17.687 ± 0.278

13.987 ± 0.271

8.736 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.356 ± 0.06

14.499 ± 0.341

1.815 ± 0.081

1.031 ± 0.066

2.251 ± 0.104

5.731 ± 0.146

2.714 ± 0.116

2.775 ± 0.13

0.463 ± 0.049

6.181 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski