endosymbiont of Rhynchophorus ferrugineus
Average proteome isoelectric point is 8.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 196 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5TGQ3|A0A2Z5TGQ3_9GAMM RNA polymerase sigma factor RpoD OS=endosymbiont of Rhynchophorus ferrugineus OX=1972133 GN=rpoD PE=3 SV=1
MM1 pKa = 7.14 TSKK4 pKa = 10.27 EE5 pKa = 4.11 VKK7 pKa = 9.67 FGNDD11 pKa = 2.87 ARR13 pKa = 11.84 IKK15 pKa = 9.5 MLNGVNILANAVKK28 pKa = 9.39 VTLGPKK34 pKa = 9.31 GRR36 pKa = 11.84 NVVLDD41 pKa = 3.8 KK42 pKa = 11.57 SFGSPLITKK51 pKa = 10.1 DD52 pKa = 3.33 GVSVARR58 pKa = 11.84 EE59 pKa = 3.58 IEE61 pKa = 4.19 LEE63 pKa = 4.15 DD64 pKa = 3.41 KK65 pKa = 10.65 FEE67 pKa = 4.45 NMGAQMVKK75 pKa = 10.17 EE76 pKa = 4.36 VASKK80 pKa = 10.68 VNDD83 pKa = 3.5 MAGDD87 pKa = 3.9 GTTTATLLAQSIINEE102 pKa = 4.1 GLKK105 pKa = 10.74 AIAAGMNPMDD115 pKa = 4.49 IKK117 pKa = 10.86 RR118 pKa = 11.84 GIDD121 pKa = 3.12 KK122 pKa = 10.73 AVNYY126 pKa = 9.93 AINEE130 pKa = 4.18 LKK132 pKa = 10.34 RR133 pKa = 11.84 ISVSCSDD140 pKa = 3.91 YY141 pKa = 11.27 KK142 pKa = 11.27 SIIQVGTISANSDD155 pKa = 3.24 EE156 pKa = 5.27 NIGKK160 pKa = 10.02 LIADD164 pKa = 3.67 AMEE167 pKa = 4.29 KK168 pKa = 10.17 VGKK171 pKa = 10.01 EE172 pKa = 3.68 GVITVEE178 pKa = 4.37 EE179 pKa = 4.61 GSGLNDD185 pKa = 3.08 EE186 pKa = 4.78 LEE188 pKa = 4.45 IVEE191 pKa = 5.13 GMQFDD196 pKa = 3.76 RR197 pKa = 11.84 GYY199 pKa = 10.76 LSPYY203 pKa = 9.83 FINKK207 pKa = 8.68 PDD209 pKa = 3.91 FNIVEE214 pKa = 4.29 FDD216 pKa = 3.3 NPYY219 pKa = 10.62 ILLIDD224 pKa = 3.9 KK225 pKa = 10.17 KK226 pKa = 9.28 ITGIRR231 pKa = 11.84 EE232 pKa = 3.92 LLPILEE238 pKa = 5.49 NITKK242 pKa = 10.03 SNKK245 pKa = 9.22 PILIIAEE252 pKa = 4.62 DD253 pKa = 3.71 IEE255 pKa = 4.92 GEE257 pKa = 4.08 ALATLVVNNMRR268 pKa = 11.84 GIFRR272 pKa = 11.84 AVAVKK277 pKa = 10.65 APGFGDD283 pKa = 3.21 RR284 pKa = 11.84 RR285 pKa = 11.84 KK286 pKa = 11.2 SMLQDD291 pKa = 3.1 IAILTGGILISEE303 pKa = 4.69 EE304 pKa = 3.71 IGLDD308 pKa = 3.24 ISKK311 pKa = 7.65 TTLKK315 pKa = 10.97 DD316 pKa = 3.03 LGNARR321 pKa = 11.84 KK322 pKa = 9.85 IIVDD326 pKa = 3.76 KK327 pKa = 11.43 DD328 pKa = 3.1 NTTIIDD334 pKa = 4.02 GYY336 pKa = 10.82 GDD338 pKa = 3.63 KK339 pKa = 10.93 KK340 pKa = 10.99 CINQRR345 pKa = 11.84 INQINKK351 pKa = 9.46 EE352 pKa = 3.96 KK353 pKa = 10.38 EE354 pKa = 4.17 QTDD357 pKa = 3.71 SEE359 pKa = 4.34 YY360 pKa = 11.24 DD361 pKa = 3.37 KK362 pKa = 11.5 EE363 pKa = 4.12 KK364 pKa = 10.11 LQEE367 pKa = 4.59 RR368 pKa = 11.84 IAKK371 pKa = 10.18 LSGGVALIRR380 pKa = 11.84 VGAATEE386 pKa = 4.09 VEE388 pKa = 4.27 MKK390 pKa = 9.93 EE391 pKa = 3.98 KK392 pKa = 10.45 KK393 pKa = 10.29 SRR395 pKa = 11.84 VEE397 pKa = 3.85 DD398 pKa = 3.56 ALNATRR404 pKa = 11.84 AAVEE408 pKa = 4.16 EE409 pKa = 4.64 GVVAGGGVALVRR421 pKa = 11.84 VSKK424 pKa = 11.01 NLLNLKK430 pKa = 9.8 GDD432 pKa = 3.98 NEE434 pKa = 4.23 DD435 pKa = 3.3 QNVGIKK441 pKa = 10.09 VAIKK445 pKa = 10.69 SMEE448 pKa = 4.05 SPLRR452 pKa = 11.84 QIVSNAGEE460 pKa = 4.19 EE461 pKa = 3.94 PSIILNKK468 pKa = 10.1 IKK470 pKa = 10.79 LSDD473 pKa = 3.59 GNFGYY478 pKa = 10.88 NAFTEE483 pKa = 4.95 EE484 pKa = 4.31 YY485 pKa = 10.8 GDD487 pKa = 4.04 MIKK490 pKa = 9.78 MGILDD495 pKa = 3.78 PTKK498 pKa = 8.92 VTRR501 pKa = 11.84 SALQYY506 pKa = 8.67 AASVAGLMITTEE518 pKa = 4.08 CMITEE523 pKa = 4.32 IKK525 pKa = 10.53 KK526 pKa = 10.8 EE527 pKa = 3.71 EE528 pKa = 4.42 DD529 pKa = 3.82 KK530 pKa = 11.69 NFDD533 pKa = 3.67 TQNPNMNNMNNIMM546 pKa = 4.24
Molecular weight: 59.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.164
IPC2_protein 5.169
IPC_protein 5.08
Toseland 4.927
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.156
Wikipedia 4.851
Rodwell 4.914
Grimsley 4.838
Solomon 5.003
Lehninger 4.952
Nozaki 5.105
DTASelect 5.245
Thurlkill 4.914
EMBOSS 4.863
Sillero 5.181
Patrickios 4.596
IPC_peptide 5.016
IPC2_peptide 5.169
IPC2.peptide.svr19 5.27
Protein with the highest isoelectric point:
>tr|A0A2Z5T9C2|A0A2Z5T9C2_9GAMM UDP-N-acetylenolpyruvoylglucosamine reductase OS=endosymbiont of Rhynchophorus ferrugineus OX=1972133 GN=murB PE=3 SV=1
MM1 pKa = 7.57 SRR3 pKa = 11.84 GHH5 pKa = 6.04 YY6 pKa = 10.48 KK7 pKa = 9.6 MLFKK11 pKa = 10.75 NLCNSLIKK19 pKa = 10.42 YY20 pKa = 7.98 EE21 pKa = 4.45 KK22 pKa = 10.09 IKK24 pKa = 8.54 TTIKK28 pKa = 9.77 RR29 pKa = 11.84 AKK31 pKa = 8.86 EE32 pKa = 3.44 IKK34 pKa = 10.25 KK35 pKa = 10.08 IIEE38 pKa = 4.41 PIINRR43 pKa = 11.84 SKK45 pKa = 11.55 VNILHH50 pKa = 6.58 NKK52 pKa = 8.9 RR53 pKa = 11.84 IVYY56 pKa = 10.21 SYY58 pKa = 11.34 LNNRR62 pKa = 11.84 NNVYY66 pKa = 10.93 KK67 pKa = 10.73 LFNDD71 pKa = 2.87 IGYY74 pKa = 9.94 RR75 pKa = 11.84 YY76 pKa = 9.96 KK77 pKa = 10.87 NRR79 pKa = 11.84 NGGYY83 pKa = 7.48 TRR85 pKa = 11.84 INKK88 pKa = 9.81 LYY90 pKa = 9.2 YY91 pKa = 10.15 RR92 pKa = 11.84 KK93 pKa = 9.95 GDD95 pKa = 3.2 SSLIVCISLVV105 pKa = 3.26
Molecular weight: 12.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.955
IPC_protein 10.204
Toseland 10.409
ProMoST 10.072
Dawson 10.584
Bjellqvist 10.248
Wikipedia 10.745
Rodwell 11.096
Grimsley 10.643
Solomon 10.599
Lehninger 10.57
Nozaki 10.394
DTASelect 10.248
Thurlkill 10.438
EMBOSS 10.804
Sillero 10.482
Patrickios 10.789
IPC_peptide 10.599
IPC2_peptide 9.033
IPC2.peptide.svr19 8.593
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
196
0
196
62158
37
1338
317.1
37.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
1.369 ± 0.115
1.03 ± 0.047
3.94 ± 0.122
4.056 ± 0.144
5.64 ± 0.189
3.758 ± 0.182
0.981 ± 0.043
17.687 ± 0.278
13.987 ± 0.271
8.736 ± 0.15
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.356 ± 0.06
14.499 ± 0.341
1.815 ± 0.081
1.031 ± 0.066
2.251 ± 0.104
5.731 ± 0.146
2.714 ± 0.116
2.775 ± 0.13
0.463 ± 0.049
6.181 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here