Nicoletella semolina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Nicoletella

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R2N5Z5|A0A4R2N5Z5_9PAST Uncharacterized protein (Fragment) OS=Nicoletella semolina OX=271160 GN=EV693_1121 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 2.88IQIPLIFTDD12 pKa = 3.82AAANKK17 pKa = 9.53VKK19 pKa = 9.77TLIEE23 pKa = 4.53GEE25 pKa = 4.35DD26 pKa = 3.71NPNLRR31 pKa = 11.84LRR33 pKa = 11.84VYY35 pKa = 9.12ITGGGCSGFQYY46 pKa = 11.03GFTFDD51 pKa = 4.96DD52 pKa = 4.19QINDD56 pKa = 3.37GDD58 pKa = 4.16LTIEE62 pKa = 4.06NQNVGLVVDD71 pKa = 4.64PMSLQYY77 pKa = 11.06LIGGTVDD84 pKa = 3.42YY85 pKa = 11.67VEE87 pKa = 5.15GLDD90 pKa = 3.57GSRR93 pKa = 11.84FLVTNPNASSTCGCGASFSII113 pKa = 5.14

Molecular weight: 12.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2SK44|A0A4V2SK44_9PAST Uncharacterized protein OS=Nicoletella semolina OX=271160 GN=EV693_10372 PE=4 SV=1
MM1 pKa = 7.52LRR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.85RR6 pKa = 11.84KK7 pKa = 9.37LKK9 pKa = 10.55QKK11 pKa = 9.99VKK13 pKa = 10.42RR14 pKa = 11.84LSSNKK19 pKa = 9.06ARR21 pKa = 11.84RR22 pKa = 11.84ILRR25 pKa = 11.84IHH27 pKa = 6.01RR28 pKa = 11.84LKK30 pKa = 10.76RR31 pKa = 11.84RR32 pKa = 11.84IFANHH37 pKa = 7.29RR38 pKa = 11.84IGFMMWQQQ46 pKa = 2.95

Molecular weight: 5.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1837

0

1837

572422

28

2810

311.6

34.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.259 ± 0.058

1.007 ± 0.021

4.85 ± 0.045

6.257 ± 0.065

4.436 ± 0.047

6.565 ± 0.055

2.15 ± 0.026

7.208 ± 0.052

6.064 ± 0.05

10.628 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.03

4.863 ± 0.052

3.701 ± 0.036

5.066 ± 0.051

4.559 ± 0.045

6.003 ± 0.045

5.245 ± 0.042

6.532 ± 0.052

1.124 ± 0.023

3.156 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski