Amino acid dipepetide frequency for Nicoletella semolina

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.079AlaAla: 6.079 ± 0.138
0.982AlaCys: 0.982 ± 0.042
4.165AlaAsp: 4.165 ± 0.097
5.721AlaGlu: 5.721 ± 0.112
3.485AlaPhe: 3.485 ± 0.085
5.461AlaGly: 5.461 ± 0.108
1.453AlaHis: 1.453 ± 0.048
6.614AlaIle: 6.614 ± 0.126
5.856AlaLys: 5.856 ± 0.114
9.255AlaLeu: 9.255 ± 0.147
2.194AlaMet: 2.194 ± 0.068
3.819AlaAsn: 3.819 ± 0.089
2.248AlaPro: 2.248 ± 0.058
4.053AlaGln: 4.053 ± 0.086
3.543AlaArg: 3.543 ± 0.086
4.222AlaSer: 4.222 ± 0.082
4.458AlaThr: 4.458 ± 0.124
5.81AlaVal: 5.81 ± 0.112
0.777AlaTrp: 0.777 ± 0.04
2.392AlaTyr: 2.392 ± 0.065
0.0AlaXaa: 0.0 ± 0.0
Cys
0.704CysAla: 0.704 ± 0.036
0.157CysCys: 0.157 ± 0.017
0.587CysAsp: 0.587 ± 0.032
0.655CysGlu: 0.655 ± 0.039
0.452CysPhe: 0.452 ± 0.034
0.891CysGly: 0.891 ± 0.044
0.307CysHis: 0.307 ± 0.027
0.66CysIle: 0.66 ± 0.036
0.498CysLys: 0.498 ± 0.028
1.029CysLeu: 1.029 ± 0.045
0.152CysMet: 0.152 ± 0.017
0.39CysAsn: 0.39 ± 0.024
0.421CysPro: 0.421 ± 0.032
0.563CysGln: 0.563 ± 0.035
0.423CysArg: 0.423 ± 0.03
0.62CysSer: 0.62 ± 0.032
0.442CysThr: 0.442 ± 0.029
0.608CysVal: 0.608 ± 0.032
0.115CysTrp: 0.115 ± 0.013
0.397CysTyr: 0.397 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.036AspAla: 3.036 ± 0.078
0.517AspCys: 0.517 ± 0.032
2.337AspAsp: 2.337 ± 0.062
3.524AspGlu: 3.524 ± 0.081
2.505AspPhe: 2.505 ± 0.069
3.103AspGly: 3.103 ± 0.09
0.858AspHis: 0.858 ± 0.039
3.794AspIle: 3.794 ± 0.087
3.199AspLys: 3.199 ± 0.085
5.152AspLeu: 5.152 ± 0.096
1.043AspMet: 1.043 ± 0.043
2.507AspAsn: 2.507 ± 0.086
2.051AspPro: 2.051 ± 0.054
1.612AspGln: 1.612 ± 0.05
2.261AspArg: 2.261 ± 0.064
2.586AspSer: 2.586 ± 0.057
2.173AspThr: 2.173 ± 0.06
3.366AspVal: 3.366 ± 0.077
0.732AspTrp: 0.732 ± 0.036
2.145AspTyr: 2.145 ± 0.073
0.0AspXaa: 0.0 ± 0.0
Glu
5.005GluAla: 5.005 ± 0.123
0.5GluCys: 0.5 ± 0.031
2.441GluAsp: 2.441 ± 0.074
3.635GluGlu: 3.635 ± 0.104
2.474GluPhe: 2.474 ± 0.062
3.462GluGly: 3.462 ± 0.084
1.272GluHis: 1.272 ± 0.051
4.776GluIle: 4.776 ± 0.094
5.127GluLys: 5.127 ± 0.108
6.493GluLeu: 6.493 ± 0.124
1.937GluMet: 1.937 ± 0.058
3.655GluAsn: 3.655 ± 0.088
1.771GluPro: 1.771 ± 0.05
4.409GluGln: 4.409 ± 0.098
3.391GluArg: 3.391 ± 0.101
2.898GluSer: 2.898 ± 0.077
2.916GluThr: 2.916 ± 0.076
3.948GluVal: 3.948 ± 0.097
0.716GluTrp: 0.716 ± 0.038
1.742GluTyr: 1.742 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.798PheAla: 3.798 ± 0.098
0.606PheCys: 0.606 ± 0.031
2.701PheAsp: 2.701 ± 0.072
2.612PheGlu: 2.612 ± 0.063
2.006PhePhe: 2.006 ± 0.072
3.29PheGly: 3.29 ± 0.081
0.917PheHis: 0.917 ± 0.037
3.41PheIle: 3.41 ± 0.095
2.159PheLys: 2.159 ± 0.072
4.042PheLeu: 4.042 ± 0.101
0.954PheMet: 0.954 ± 0.044
2.294PheAsn: 2.294 ± 0.066
1.607PhePro: 1.607 ± 0.06
1.609PheGln: 1.609 ± 0.056
1.635PheArg: 1.635 ± 0.058
3.504PheSer: 3.504 ± 0.079
2.341PheThr: 2.341 ± 0.076
2.783PheVal: 2.783 ± 0.075
0.512PheTrp: 0.512 ± 0.034
1.576PheTyr: 1.576 ± 0.057
0.0PheXaa: 0.0 ± 0.0
Gly
4.738GlyAla: 4.738 ± 0.12
0.746GlyCys: 0.746 ± 0.038
3.096GlyAsp: 3.096 ± 0.107
4.406GlyGlu: 4.406 ± 0.094
3.092GlyPhe: 3.092 ± 0.077
4.505GlyGly: 4.505 ± 0.111
1.3GlyHis: 1.3 ± 0.053
5.442GlyIle: 5.442 ± 0.102
5.033GlyLys: 5.033 ± 0.103
6.604GlyLeu: 6.604 ± 0.108
1.661GlyMet: 1.661 ± 0.049
2.83GlyAsn: 2.83 ± 0.083
1.059GlyPro: 1.059 ± 0.044
2.558GlyGln: 2.558 ± 0.076
3.031GlyArg: 3.031 ± 0.085
3.667GlySer: 3.667 ± 0.085
3.428GlyThr: 3.428 ± 0.08
5.052GlyVal: 5.052 ± 0.106
0.842GlyTrp: 0.842 ± 0.039
2.566GlyTyr: 2.566 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.23HisAla: 1.23 ± 0.047
0.362HisCys: 0.362 ± 0.026
0.825HisAsp: 0.825 ± 0.042
0.758HisGlu: 0.758 ± 0.038
1.331HisPhe: 1.331 ± 0.047
1.136HisGly: 1.136 ± 0.052
0.741HisHis: 0.741 ± 0.041
1.71HisIle: 1.71 ± 0.055
1.198HisLys: 1.198 ± 0.048
2.579HisLeu: 2.579 ± 0.076
0.309HisMet: 0.309 ± 0.024
1.02HisAsn: 1.02 ± 0.043
0.94HisPro: 0.94 ± 0.048
1.343HisGln: 1.343 ± 0.054
1.094HisArg: 1.094 ± 0.044
1.665HisSer: 1.665 ± 0.053
1.23HisThr: 1.23 ± 0.051
0.687HisVal: 0.687 ± 0.038
0.313HisTrp: 0.313 ± 0.024
1.034HisTyr: 1.034 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
7.073IleAla: 7.073 ± 0.123
0.832IleCys: 0.832 ± 0.047
4.132IleAsp: 4.132 ± 0.085
4.993IleGlu: 4.993 ± 0.093
3.04IlePhe: 3.04 ± 0.094
5.264IleGly: 5.264 ± 0.102
1.422IleHis: 1.422 ± 0.048
4.745IleIle: 4.745 ± 0.114
3.789IleLys: 3.789 ± 0.099
6.827IleLeu: 6.827 ± 0.145
1.336IleMet: 1.336 ± 0.052
3.476IleAsn: 3.476 ± 0.1
2.739IlePro: 2.739 ± 0.077
2.9IleGln: 2.9 ± 0.061
3.23IleArg: 3.23 ± 0.08
4.939IleSer: 4.939 ± 0.092
4.049IleThr: 4.049 ± 0.114
4.553IleVal: 4.553 ± 0.099
0.709IleTrp: 0.709 ± 0.032
2.03IleTyr: 2.03 ± 0.063
0.0IleXaa: 0.0 ± 0.0
Lys
5.234LysAla: 5.234 ± 0.111
0.353LysCys: 0.353 ± 0.026
2.517LysAsp: 2.517 ± 0.08
3.742LysGlu: 3.742 ± 0.09
2.009LysPhe: 2.009 ± 0.064
3.794LysGly: 3.794 ± 0.081
1.163LysHis: 1.163 ± 0.042
4.395LysIle: 4.395 ± 0.105
3.796LysLys: 3.796 ± 0.088
6.235LysLeu: 6.235 ± 0.119
1.929LysMet: 1.929 ± 0.061
3.363LysAsn: 3.363 ± 0.089
2.292LysPro: 2.292 ± 0.068
3.527LysGln: 3.527 ± 0.08
3.064LysArg: 3.064 ± 0.083
3.41LysSer: 3.41 ± 0.082
3.426LysThr: 3.426 ± 0.091
4.025LysVal: 4.025 ± 0.098
0.666LysTrp: 0.666 ± 0.03
1.703LysTyr: 1.703 ± 0.067
0.0LysXaa: 0.0 ± 0.0
Leu
9.788LeuAla: 9.788 ± 0.171
1.053LeuCys: 1.053 ± 0.044
5.527LeuAsp: 5.527 ± 0.1
6.186LeuGlu: 6.186 ± 0.117
4.883LeuPhe: 4.883 ± 0.123
7.049LeuGly: 7.049 ± 0.128
2.152LeuHis: 2.152 ± 0.059
6.752LeuIle: 6.752 ± 0.142
5.893LeuLys: 5.893 ± 0.112
10.976LeuLeu: 10.976 ± 0.208
2.425LeuMet: 2.425 ± 0.065
5.496LeuAsn: 5.496 ± 0.099
4.862LeuPro: 4.862 ± 0.111
4.469LeuGln: 4.469 ± 0.093
4.671LeuArg: 4.671 ± 0.107
7.65LeuSer: 7.65 ± 0.125
6.146LeuThr: 6.146 ± 0.102
6.558LeuVal: 6.558 ± 0.113
1.066LeuTrp: 1.066 ± 0.053
2.676LeuTyr: 2.676 ± 0.069
0.0LeuXaa: 0.0 ± 0.0
Met
2.234MetAla: 2.234 ± 0.066
0.189MetCys: 0.189 ± 0.018
0.853MetAsp: 0.853 ± 0.038
1.101MetGlu: 1.101 ± 0.043
0.861MetPhe: 0.861 ± 0.039
1.59MetGly: 1.59 ± 0.061
0.367MetHis: 0.367 ± 0.027
1.471MetIle: 1.471 ± 0.05
1.534MetLys: 1.534 ± 0.049
2.629MetLeu: 2.629 ± 0.075
0.73MetMet: 0.73 ± 0.037
1.15MetAsn: 1.15 ± 0.04
1.069MetPro: 1.069 ± 0.043
1.315MetGln: 1.315 ± 0.053
1.109MetArg: 1.109 ± 0.039
1.506MetSer: 1.506 ± 0.047
1.37MetThr: 1.37 ± 0.056
1.495MetVal: 1.495 ± 0.049
0.224MetTrp: 0.224 ± 0.02
0.486MetTyr: 0.486 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
4.249AsnAla: 4.249 ± 0.103
0.444AsnCys: 0.444 ± 0.029
2.316AsnAsp: 2.316 ± 0.07
2.827AsnGlu: 2.827 ± 0.075
1.957AsnPhe: 1.957 ± 0.067
3.412AsnGly: 3.412 ± 0.09
1.118AsnHis: 1.118 ± 0.043
3.721AsnIle: 3.721 ± 0.099
2.673AsnLys: 2.673 ± 0.08
5.04AsnLeu: 5.04 ± 0.099
0.942AsnMet: 0.942 ± 0.041
2.323AsnAsn: 2.323 ± 0.074
2.414AsnPro: 2.414 ± 0.066
2.509AsnGln: 2.509 ± 0.077
2.137AsnArg: 2.137 ± 0.067
2.732AsnSer: 2.732 ± 0.078
2.434AsnThr: 2.434 ± 0.073
3.202AsnVal: 3.202 ± 0.073
0.566AsnTrp: 0.566 ± 0.034
1.614AsnTyr: 1.614 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
2.834ProAla: 2.834 ± 0.077
0.281ProCys: 0.281 ± 0.023
1.883ProAsp: 1.883 ± 0.061
2.823ProGlu: 2.823 ± 0.072
1.892ProPhe: 1.892 ± 0.062
1.113ProGly: 1.113 ± 0.052
0.975ProHis: 0.975 ± 0.039
2.856ProIle: 2.856 ± 0.071
2.334ProLys: 2.334 ± 0.067
3.896ProLeu: 3.896 ± 0.089
0.926ProMet: 0.926 ± 0.038
2.346ProAsn: 2.346 ± 0.065
1.064ProPro: 1.064 ± 0.051
1.848ProGln: 1.848 ± 0.064
1.317ProArg: 1.317 ± 0.053
2.151ProSer: 2.151 ± 0.064
2.308ProThr: 2.308 ± 0.058
2.446ProVal: 2.446 ± 0.069
0.367ProTrp: 0.367 ± 0.028
1.347ProTyr: 1.347 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
4.937GlnAla: 4.937 ± 0.102
0.391GlnCys: 0.391 ± 0.029
2.042GlnAsp: 2.042 ± 0.066
2.704GlnGlu: 2.704 ± 0.083
2.323GlnPhe: 2.323 ± 0.066
3.202GlnGly: 3.202 ± 0.088
1.284GlnHis: 1.284 ± 0.049
3.534GlnIle: 3.534 ± 0.076
3.05GlnLys: 3.05 ± 0.083
5.41GlnLeu: 5.41 ± 0.125
1.066GlnMet: 1.066 ± 0.042
2.42GlnAsn: 2.42 ± 0.082
1.85GlnPro: 1.85 ± 0.051
3.655GlnGln: 3.655 ± 0.117
2.47GlnArg: 2.47 ± 0.079
2.799GlnSer: 2.799 ± 0.064
2.484GlnThr: 2.484 ± 0.07
2.905GlnVal: 2.905 ± 0.069
0.631GlnTrp: 0.631 ± 0.03
1.504GlnTyr: 1.504 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
3.153ArgAla: 3.153 ± 0.075
0.491ArgCys: 0.491 ± 0.03
2.22ArgAsp: 2.22 ± 0.072
3.234ArgGlu: 3.234 ± 0.096
2.378ArgPhe: 2.378 ± 0.067
2.648ArgGly: 2.648 ± 0.081
1.118ArgHis: 1.118 ± 0.048
3.117ArgIle: 3.117 ± 0.08
2.61ArgLys: 2.61 ± 0.074
5.234ArgLeu: 5.234 ± 0.119
1.024ArgMet: 1.024 ± 0.038
2.039ArgAsn: 2.039 ± 0.058
1.639ArgPro: 1.639 ± 0.056
2.657ArgGln: 2.657 ± 0.081
2.219ArgArg: 2.219 ± 0.075
2.376ArgSer: 2.376 ± 0.07
2.131ArgThr: 2.131 ± 0.059
2.896ArgVal: 2.896 ± 0.078
0.573ArgTrp: 0.573 ± 0.032
1.829ArgTyr: 1.829 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
4.736SerAla: 4.736 ± 0.087
0.575SerCys: 0.575 ± 0.034
2.94SerAsp: 2.94 ± 0.075
3.7SerGlu: 3.7 ± 0.085
2.619SerPhe: 2.619 ± 0.071
4.551SerGly: 4.551 ± 0.102
1.492SerHis: 1.492 ± 0.053
3.995SerIle: 3.995 ± 0.102
3.043SerLys: 3.043 ± 0.079
6.628SerLeu: 6.628 ± 0.123
1.242SerMet: 1.242 ± 0.05
2.64SerAsn: 2.64 ± 0.071
2.488SerPro: 2.488 ± 0.076
3.124SerGln: 3.124 ± 0.085
2.636SerArg: 2.636 ± 0.074
3.73SerSer: 3.73 ± 0.1
3.117SerThr: 3.117 ± 0.09
4.041SerVal: 4.041 ± 0.083
0.688SerTrp: 0.688 ± 0.035
2.044SerTyr: 2.044 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
4.64ThrAla: 4.64 ± 0.108
0.367ThrCys: 0.367 ± 0.031
2.54ThrAsp: 2.54 ± 0.067
3.185ThrGlu: 3.185 ± 0.076
2.278ThrPhe: 2.278 ± 0.055
3.878ThrGly: 3.878 ± 0.103
1.314ThrHis: 1.314 ± 0.053
3.63ThrIle: 3.63 ± 0.083
2.734ThrLys: 2.734 ± 0.078
6.298ThrLeu: 6.298 ± 0.131
1.064ThrMet: 1.064 ± 0.049
2.084ThrAsn: 2.084 ± 0.066
2.47ThrPro: 2.47 ± 0.068
2.731ThrGln: 2.731 ± 0.07
2.072ThrArg: 2.072 ± 0.066
2.645ThrSer: 2.645 ± 0.06
2.98ThrThr: 2.98 ± 0.075
3.597ThrVal: 3.597 ± 0.111
0.533ThrTrp: 0.533 ± 0.033
1.406ThrTyr: 1.406 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
5.817ValAla: 5.817 ± 0.103
0.688ValCys: 0.688 ± 0.04
3.333ValAsp: 3.333 ± 0.085
4.67ValGlu: 4.67 ± 0.097
2.605ValPhe: 2.605 ± 0.069
4.511ValGly: 4.511 ± 0.108
1.073ValHis: 1.073 ± 0.049
4.879ValIle: 4.879 ± 0.089
4.119ValLys: 4.119 ± 0.104
6.544ValLeu: 6.544 ± 0.116
1.612ValMet: 1.612 ± 0.059
3.048ValAsn: 3.048 ± 0.081
2.402ValPro: 2.402 ± 0.072
2.582ValGln: 2.582 ± 0.071
2.863ValArg: 2.863 ± 0.084
4.374ValSer: 4.374 ± 0.099
3.015ValThr: 3.015 ± 0.083
4.855ValVal: 4.855 ± 0.11
0.601ValTrp: 0.601 ± 0.034
1.726ValTyr: 1.726 ± 0.06
0.0ValXaa: 0.0 ± 0.0
Trp
0.788TrpAla: 0.788 ± 0.036
0.145TrpCys: 0.145 ± 0.016
0.48TrpAsp: 0.48 ± 0.032
0.601TrpGlu: 0.601 ± 0.036
0.542TrpPhe: 0.542 ± 0.03
0.708TrpGly: 0.708 ± 0.036
0.302TrpHis: 0.302 ± 0.022
0.779TrpIle: 0.779 ± 0.041
0.59TrpLys: 0.59 ± 0.028
1.702TrpLeu: 1.702 ± 0.061
0.148TrpMet: 0.148 ± 0.017
0.419TrpAsn: 0.419 ± 0.028
0.148TrpPro: 0.148 ± 0.017
1.029TrpGln: 1.029 ± 0.046
0.608TrpArg: 0.608 ± 0.032
0.517TrpSer: 0.517 ± 0.033
0.466TrpThr: 0.466 ± 0.029
0.779TrpVal: 0.779 ± 0.032
0.182TrpTrp: 0.182 ± 0.017
0.307TrpTyr: 0.307 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.516TyrAla: 2.516 ± 0.07
0.393TyrCys: 0.393 ± 0.028
1.565TyrAsp: 1.565 ± 0.059
1.494TyrGlu: 1.494 ± 0.05
1.625TyrPhe: 1.625 ± 0.055
2.088TyrGly: 2.088 ± 0.057
0.875TyrHis: 0.875 ± 0.04
1.796TyrIle: 1.796 ± 0.061
1.408TyrLys: 1.408 ± 0.057
3.705TyrLeu: 3.705 ± 0.09
0.585TyrMet: 0.585 ± 0.035
1.31TyrAsn: 1.31 ± 0.053
1.471TyrPro: 1.471 ± 0.058
2.17TyrGln: 2.17 ± 0.064
1.812TyrArg: 1.812 ± 0.059
2.039TyrSer: 2.039 ± 0.066
1.532TyrThr: 1.532 ± 0.051
1.712TyrVal: 1.712 ± 0.049
0.43TyrTrp: 0.43 ± 0.03
1.032TyrTyr: 1.032 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1837 proteins (572422 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski