Rhodospirillales bacterium
Average proteome isoelectric point is 7.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7065 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A7MGS6|A0A6A7MGS6_9PROT DNA polymerase III subunit gamma/tau OS=Rhodospirillales bacterium OX=2026786 GN=dnaX PE=3 SV=1
MM1 pKa = 7.33 PQRR4 pKa = 11.84 TSRR7 pKa = 11.84 SSALTRR13 pKa = 11.84 STSSQTISCCDD24 pKa = 3.62 LLHH27 pKa = 6.58 TEE29 pKa = 4.54 TRR31 pKa = 11.84 QDD33 pKa = 3.48 DD34 pKa = 3.54 HH35 pKa = 7.73 GKK37 pKa = 10.01 ALNVSVSRR45 pKa = 11.84 KK46 pKa = 9.15 DD47 pKa = 3.11 AAMPIIKK54 pKa = 10.1 FGTFGNDD61 pKa = 3.21 TLVGTADD68 pKa = 3.73 SDD70 pKa = 3.52 WLYY73 pKa = 11.64 GLGGDD78 pKa = 4.89 DD79 pKa = 3.77 VLSGGGGEE87 pKa = 5.08 DD88 pKa = 4.01 YY89 pKa = 11.28 LSGGDD94 pKa = 4.74 DD95 pKa = 3.49 NDD97 pKa = 3.36 VLYY100 pKa = 11.09 GGDD103 pKa = 3.56 EE104 pKa = 4.27 DD105 pKa = 4.58 PRR107 pKa = 11.84 HH108 pKa = 6.04 GDD110 pKa = 3.57 EE111 pKa = 5.64 LDD113 pKa = 4.25 GGDD116 pKa = 3.99 GDD118 pKa = 4.46 DD119 pKa = 3.99 WLFGGAGGDD128 pKa = 3.7 HH129 pKa = 7.01 LLGGAGNDD137 pKa = 3.65 HH138 pKa = 7.27 LYY140 pKa = 11.2 GGADD144 pKa = 3.36 NDD146 pKa = 4.18 YY147 pKa = 11.13 LDD149 pKa = 4.69 GGAGADD155 pKa = 3.63 VMDD158 pKa = 4.87 GGEE161 pKa = 3.94 GLDD164 pKa = 3.08 RR165 pKa = 11.84 VEE167 pKa = 5.02 YY168 pKa = 9.35 IHH170 pKa = 6.91 SPAAVTVNLTTGTGSGGDD188 pKa = 3.47 AAGDD192 pKa = 3.64 TLTDD196 pKa = 3.37 IEE198 pKa = 4.99 DD199 pKa = 3.87 VIGSDD204 pKa = 4.81 FADD207 pKa = 3.47 TLIGNGSSNRR217 pKa = 11.84 LTGGSGDD224 pKa = 4.68 DD225 pKa = 3.76 YY226 pKa = 11.54 LSGSGGDD233 pKa = 4.07 DD234 pKa = 3.58 FLHH237 pKa = 6.69 GGQGADD243 pKa = 3.6 HH244 pKa = 6.58 LHH246 pKa = 6.54 GGSGSDD252 pKa = 3.14 TVSYY256 pKa = 9.43 TGSAAGVTVNLEE268 pKa = 4.16 ANVVSGGDD276 pKa = 3.5 AEE278 pKa = 5.38 GDD280 pKa = 3.49 TLVSIEE286 pKa = 4.28 NVVGSTGADD295 pKa = 3.33 VITGNAEE302 pKa = 3.86 ANILSGSSGDD312 pKa = 3.75 DD313 pKa = 3.09 RR314 pKa = 11.84 LNGGAGDD321 pKa = 3.81 DD322 pKa = 4.28 TLNGGQGQDD331 pKa = 3.94 DD332 pKa = 5.33 LIGGDD337 pKa = 3.67 GADD340 pKa = 2.77 TFVFSEE346 pKa = 3.89 HH347 pKa = 6.3 WYY349 pKa = 9.42 YY350 pKa = 11.25 SPPPVSDD357 pKa = 3.61 TSVEE361 pKa = 4.01 APDD364 pKa = 4.32 YY365 pKa = 11.01 IWDD368 pKa = 3.94 FNHH371 pKa = 6.95 AQGDD375 pKa = 4.18 QIDD378 pKa = 4.5 LADD381 pKa = 4.64 LDD383 pKa = 4.45 AVSGVPGDD391 pKa = 3.56 QAFSFINTADD401 pKa = 3.66 FTGSEE406 pKa = 4.23 GEE408 pKa = 3.87 LRR410 pKa = 11.84 YY411 pKa = 9.85 EE412 pKa = 4.05 QFTGPFGITWTAVSGDD428 pKa = 3.5 MDD430 pKa = 4.24 GDD432 pKa = 3.89 AVADD436 pKa = 3.98 FAIVCTDD443 pKa = 4.02 SINFVASDD451 pKa = 3.61 FLLL454 pKa = 4.64
Molecular weight: 46.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.503
IPC_protein 3.567
Toseland 3.325
ProMoST 3.757
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.567
Rodwell 3.389
Grimsley 3.223
Solomon 3.579
Lehninger 3.541
Nozaki 3.694
DTASelect 4.024
Thurlkill 3.389
EMBOSS 3.567
Sillero 3.694
Patrickios 1.354
IPC_peptide 3.579
IPC2_peptide 3.668
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|A0A6A7LWW9|A0A6A7LWW9_9PROT Glutathione S-transferase family protein OS=Rhodospirillales bacterium OX=2026786 GN=GEV13_00565 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.33 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 SGRR39 pKa = 11.84 KK40 pKa = 8.96 RR41 pKa = 11.84 LSAA44 pKa = 4.09
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7065
0
7065
2124564
29
3930
300.7
32.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.536 ± 0.039
0.926 ± 0.009
5.513 ± 0.023
5.287 ± 0.026
3.654 ± 0.018
8.748 ± 0.034
2.118 ± 0.014
4.755 ± 0.021
3.386 ± 0.03
10.062 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.482 ± 0.015
2.49 ± 0.018
5.534 ± 0.02
3.061 ± 0.017
7.628 ± 0.037
5.11 ± 0.021
5.208 ± 0.024
7.692 ± 0.025
1.541 ± 0.012
2.268 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here