Amino acid dipepetide frequency for Rhodospirillales bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.685AlaAla: 17.685 ± 0.135	1.181AlaCys: 1.181 ± 0.025	6.871AlaAsp: 6.871 ± 0.067	7.08AlaGlu: 7.08 ± 0.065	4.565AlaPhe: 4.565 ± 0.056	10.942AlaGly: 10.942 ± 0.089	2.282AlaHis: 2.282 ± 0.035	6.109AlaIle: 6.109 ± 0.06	4.255AlaLys: 4.255 ± 0.056	13.234AlaLeu: 13.234 ± 0.098	3.571AlaMet: 3.571 ± 0.041	2.972AlaAsn: 2.972 ± 0.041	5.846AlaPro: 5.846 ± 0.059	3.72AlaGln: 3.72 ± 0.049	9.335AlaArg: 9.335 ± 0.084	6.042AlaSer: 6.042 ± 0.054	6.137AlaThr: 6.137 ± 0.061	9.098AlaVal: 9.098 ± 0.066	1.844AlaTrp: 1.844 ± 0.033	2.586AlaTyr: 2.586 ± 0.038	0.0AlaXaa: 0.0 ± 0.0
Cys	0.989CysAla: 0.989 ± 0.023	0.146CysCys: 0.146 ± 0.009	0.55CysAsp: 0.55 ± 0.018	0.448CysGlu: 0.448 ± 0.016	0.371CysPhe: 0.371 ± 0.014	0.995CysGly: 0.995 ± 0.024	0.266CysHis: 0.266 ± 0.011	0.406CysIle: 0.406 ± 0.013	0.242CysLys: 0.242 ± 0.012	0.844CysLeu: 0.844 ± 0.023	0.18CysMet: 0.18 ± 0.009	0.229CysAsn: 0.229 ± 0.012	0.484CysPro: 0.484 ± 0.016	0.261CysGln: 0.261 ± 0.01	0.834CysArg: 0.834 ± 0.02	0.523CysSer: 0.523 ± 0.017	0.463CysThr: 0.463 ± 0.016	0.667CysVal: 0.667 ± 0.019	0.15CysTrp: 0.15 ± 0.008	0.207CysTyr: 0.207 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	6.344AspAla: 6.344 ± 0.056	0.529AspCys: 0.529 ± 0.016	2.922AspAsp: 2.922 ± 0.04	3.096AspGlu: 3.096 ± 0.041	2.096AspPhe: 2.096 ± 0.034	5.2AspGly: 5.2 ± 0.059	1.227AspHis: 1.227 ± 0.023	2.652AspIle: 2.652 ± 0.037	1.98AspLys: 1.98 ± 0.034	5.552AspLeu: 5.552 ± 0.055	1.32AspMet: 1.32 ± 0.028	1.233AspAsn: 1.233 ± 0.026	3.494AspPro: 3.494 ± 0.049	1.595AspGln: 1.595 ± 0.031	4.553AspArg: 4.553 ± 0.052	2.2AspSer: 2.2 ± 0.032	2.404AspThr: 2.404 ± 0.035	4.175AspVal: 4.175 ± 0.048	1.101AspTrp: 1.101 ± 0.027	1.459AspTyr: 1.459 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	7.035GluAla: 7.035 ± 0.057	0.357GluCys: 0.357 ± 0.014	2.214GluAsp: 2.214 ± 0.031	2.808GluGlu: 2.808 ± 0.039	1.743GluPhe: 1.743 ± 0.028	3.852GluGly: 3.852 ± 0.046	1.188GluHis: 1.188 ± 0.024	3.056GluIle: 3.056 ± 0.041	2.07GluLys: 2.07 ± 0.039	5.416GluLeu: 5.416 ± 0.054	1.392GluMet: 1.392 ± 0.024	1.218GluAsn: 1.218 ± 0.027	2.701GluPro: 2.701 ± 0.044	2.021GluGln: 2.021 ± 0.034	5.156GluArg: 5.156 ± 0.059	2.163GluSer: 2.163 ± 0.033	2.882GluThr: 2.882 ± 0.042	3.856GluVal: 3.856 ± 0.045	0.738GluTrp: 0.738 ± 0.019	1.006GluTyr: 1.006 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.667PheAla: 4.667 ± 0.053	0.402PheCys: 0.402 ± 0.015	2.597PheAsp: 2.597 ± 0.038	1.982PheGlu: 1.982 ± 0.036	1.353PhePhe: 1.353 ± 0.025	3.611PheGly: 3.611 ± 0.048	0.804PheHis: 0.804 ± 0.019	1.512PheIle: 1.512 ± 0.028	1.074PheLys: 1.074 ± 0.027	3.274PheLeu: 3.274 ± 0.045	0.781PheMet: 0.781 ± 0.019	1.083PheAsn: 1.083 ± 0.024	1.646PhePro: 1.646 ± 0.03	0.965PheGln: 0.965 ± 0.018	2.336PheArg: 2.336 ± 0.032	1.959PheSer: 1.959 ± 0.031	2.045PheThr: 2.045 ± 0.032	2.995PheVal: 2.995 ± 0.04	0.584PheTrp: 0.584 ± 0.016	0.872PheTyr: 0.872 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	9.402GlyAla: 9.402 ± 0.086	0.897GlyCys: 0.897 ± 0.022	4.197GlyAsp: 4.197 ± 0.057	4.45GlyGlu: 4.45 ± 0.05	3.527GlyPhe: 3.527 ± 0.045	7.959GlyGly: 7.959 ± 0.103	1.993GlyHis: 1.993 ± 0.034	4.297GlyIle: 4.297 ± 0.054	3.458GlyLys: 3.458 ± 0.043	8.986GlyLeu: 8.986 ± 0.075	2.33GlyMet: 2.33 ± 0.038	2.259GlyAsn: 2.259 ± 0.046	4.052GlyPro: 4.052 ± 0.049	2.856GlyGln: 2.856 ± 0.037	6.927GlyArg: 6.927 ± 0.063	4.619GlySer: 4.619 ± 0.054	4.789GlyThr: 4.789 ± 0.067	6.508GlyVal: 6.508 ± 0.052	1.637GlyTrp: 1.637 ± 0.029	2.332GlyTyr: 2.332 ± 0.033	0.0GlyXaa: 0.0 ± 0.0
His	2.405HisAla: 2.405 ± 0.035	0.271HisCys: 0.271 ± 0.012	1.236HisAsp: 1.236 ± 0.021	1.034HisGlu: 1.034 ± 0.022	0.864HisPhe: 0.864 ± 0.02	2.05HisGly: 2.05 ± 0.034	0.619HisHis: 0.619 ± 0.019	0.907HisIle: 0.907 ± 0.022	0.537HisLys: 0.537 ± 0.016	2.117HisLeu: 2.117 ± 0.033	0.464HisMet: 0.464 ± 0.014	0.505HisAsn: 0.505 ± 0.015	1.445HisPro: 1.445 ± 0.029	0.588HisGln: 0.588 ± 0.017	1.661HisArg: 1.661 ± 0.032	0.926HisSer: 0.926 ± 0.023	0.893HisThr: 0.893 ± 0.021	1.64HisVal: 1.64 ± 0.028	0.4HisTrp: 0.4 ± 0.014	0.616HisTyr: 0.616 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.884IleAla: 6.884 ± 0.069	0.526IleCys: 0.526 ± 0.016	3.575IleAsp: 3.575 ± 0.035	3.312IleGlu: 3.312 ± 0.04	1.496IlePhe: 1.496 ± 0.03	4.824IleGly: 4.824 ± 0.059	0.899IleHis: 0.899 ± 0.021	1.683IleIle: 1.683 ± 0.03	1.477IleLys: 1.477 ± 0.031	3.834IleLeu: 3.834 ± 0.049	0.892IleMet: 0.892 ± 0.021	1.262IleAsn: 1.262 ± 0.028	2.063IlePro: 2.063 ± 0.037	1.145IleGln: 1.145 ± 0.024	2.826IleArg: 2.826 ± 0.037	2.334IleSer: 2.334 ± 0.037	2.329IleThr: 2.329 ± 0.034	4.478IleVal: 4.478 ± 0.047	0.61IleTrp: 0.61 ± 0.018	1.101IleTyr: 1.101 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	4.279LysAla: 4.279 ± 0.059	0.194LysCys: 0.194 ± 0.009	1.798LysAsp: 1.798 ± 0.032	1.676LysGlu: 1.676 ± 0.033	0.977LysPhe: 0.977 ± 0.023	2.761LysGly: 2.761 ± 0.043	0.66LysHis: 0.66 ± 0.017	1.634LysIle: 1.634 ± 0.03	1.402LysLys: 1.402 ± 0.035	3.443LysLeu: 3.443 ± 0.046	0.816LysMet: 0.816 ± 0.02	0.801LysAsn: 0.801 ± 0.019	2.361LysPro: 2.361 ± 0.031	1.004LysGln: 1.004 ± 0.023	2.633LysArg: 2.633 ± 0.038	1.685LysSer: 1.685 ± 0.031	1.83LysThr: 1.83 ± 0.033	2.758LysVal: 2.758 ± 0.044	0.455LysTrp: 0.455 ± 0.016	0.698LysTyr: 0.698 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	14.042LeuAla: 14.042 ± 0.097	0.947LeuCys: 0.947 ± 0.023	5.779LeuAsp: 5.779 ± 0.063	4.958LeuGlu: 4.958 ± 0.05	3.532LeuPhe: 3.532 ± 0.048	8.83LeuGly: 8.83 ± 0.073	1.923LeuHis: 1.923 ± 0.031	4.556LeuIle: 4.556 ± 0.063	3.711LeuLys: 3.711 ± 0.049	9.741LeuLeu: 9.741 ± 0.094	2.326LeuMet: 2.326 ± 0.032	2.344LeuAsn: 2.344 ± 0.039	5.79LeuPro: 5.79 ± 0.056	2.898LeuGln: 2.898 ± 0.041	7.146LeuArg: 7.146 ± 0.065	5.91LeuSer: 5.91 ± 0.064	4.967LeuThr: 4.967 ± 0.056	7.787LeuVal: 7.787 ± 0.074	1.329LeuTrp: 1.329 ± 0.025	2.1LeuTyr: 2.1 ± 0.035	0.0LeuXaa: 0.0 ± 0.0
Met	3.456MetAla: 3.456 ± 0.039	0.187MetCys: 0.187 ± 0.011	1.055MetAsp: 1.055 ± 0.023	1.048MetGlu: 1.048 ± 0.022	0.729MetPhe: 0.729 ± 0.018	1.857MetGly: 1.857 ± 0.033	0.473MetHis: 0.473 ± 0.015	1.234MetIle: 1.234 ± 0.025	1.056MetLys: 1.056 ± 0.024	2.466MetLeu: 2.466 ± 0.039	0.627MetMet: 0.627 ± 0.02	0.686MetAsn: 0.686 ± 0.019	1.626MetPro: 1.626 ± 0.03	0.74MetGln: 0.74 ± 0.017	1.853MetArg: 1.853 ± 0.028	1.614MetSer: 1.614 ± 0.03	1.749MetThr: 1.749 ± 0.029	1.762MetVal: 1.762 ± 0.026	0.253MetTrp: 0.253 ± 0.011	0.353MetTyr: 0.353 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.959AsnAla: 2.959 ± 0.042	0.257AsnCys: 0.257 ± 0.011	1.366AsnAsp: 1.366 ± 0.03	1.209AsnGlu: 1.209 ± 0.024	0.907AsnPhe: 0.907 ± 0.024	2.336AsnGly: 2.336 ± 0.042	0.525AsnHis: 0.525 ± 0.017	1.19AsnIle: 1.19 ± 0.026	0.754AsnLys: 0.754 ± 0.022	2.384AsnLeu: 2.384 ± 0.035	0.59AsnMet: 0.59 ± 0.016	0.703AsnAsn: 0.703 ± 0.026	1.744AsnPro: 1.744 ± 0.031	0.769AsnGln: 0.769 ± 0.022	1.817AsnArg: 1.817 ± 0.031	1.086AsnSer: 1.086 ± 0.027	1.209AsnThr: 1.209 ± 0.029	2.024AsnVal: 2.024 ± 0.03	0.434AsnTrp: 0.434 ± 0.015	0.635AsnTyr: 0.635 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.809ProAla: 6.809 ± 0.065	0.363ProCys: 0.363 ± 0.012	3.621ProAsp: 3.621 ± 0.053	3.306ProGlu: 3.306 ± 0.038	2.008ProPhe: 2.008 ± 0.027	4.905ProGly: 4.905 ± 0.052	1.181ProHis: 1.181 ± 0.023	2.425ProIle: 2.425 ± 0.04	1.848ProLys: 1.848 ± 0.035	4.994ProLeu: 4.994 ± 0.046	1.357ProMet: 1.357 ± 0.024	1.379ProAsn: 1.379 ± 0.028	3.465ProPro: 3.465 ± 0.055	1.714ProGln: 1.714 ± 0.029	3.589ProArg: 3.589 ± 0.045	2.959ProSer: 2.959 ± 0.034	2.951ProThr: 2.951 ± 0.037	4.275ProVal: 4.275 ± 0.052	0.886ProTrp: 0.886 ± 0.023	1.303ProTyr: 1.303 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.137GlnAla: 4.137 ± 0.048	0.235GlnCys: 0.235 ± 0.011	1.317GlnAsp: 1.317 ± 0.025	1.33GlnGlu: 1.33 ± 0.027	1.037GlnPhe: 1.037 ± 0.019	2.402GlnGly: 2.402 ± 0.033	0.65GlnHis: 0.65 ± 0.018	1.557GlnIle: 1.557 ± 0.026	1.036GlnLys: 1.036 ± 0.026	2.841GlnLeu: 2.841 ± 0.037	0.759GlnMet: 0.759 ± 0.017	0.738GlnAsn: 0.738 ± 0.019	1.923GlnPro: 1.923 ± 0.032	1.28GlnGln: 1.28 ± 0.031	2.73GlnArg: 2.73 ± 0.038	1.645GlnSer: 1.645 ± 0.03	1.595GlnThr: 1.595 ± 0.034	2.298GlnVal: 2.298 ± 0.031	0.474GlnTrp: 0.474 ± 0.014	0.628GlnTyr: 0.628 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	8.478ArgAla: 8.478 ± 0.072	0.699ArgCys: 0.699 ± 0.02	4.166ArgAsp: 4.166 ± 0.043	4.117ArgGlu: 4.117 ± 0.05	3.019ArgPhe: 3.019 ± 0.044	5.279ArgGly: 5.279 ± 0.056	2.027ArgHis: 2.027 ± 0.031	3.826ArgIle: 3.826 ± 0.045	2.415ArgLys: 2.415 ± 0.039	8.7ArgLeu: 8.7 ± 0.089	1.936ArgMet: 1.936 ± 0.029	1.853ArgAsn: 1.853 ± 0.031	4.195ArgPro: 4.195 ± 0.046	2.841ArgGln: 2.841 ± 0.042	7.06ArgArg: 7.06 ± 0.073	3.842ArgSer: 3.842 ± 0.045	3.817ArgThr: 3.817 ± 0.039	4.966ArgVal: 4.966 ± 0.049	1.199ArgTrp: 1.199 ± 0.026	1.849ArgTyr: 1.849 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	5.878SerAla: 5.878 ± 0.057	0.477SerCys: 0.477 ± 0.015	2.733SerAsp: 2.733 ± 0.038	2.371SerGlu: 2.371 ± 0.036	2.138SerPhe: 2.138 ± 0.031	5.194SerGly: 5.194 ± 0.059	1.075SerHis: 1.075 ± 0.024	2.498SerIle: 2.498 ± 0.038	1.414SerLys: 1.414 ± 0.031	5.206SerLeu: 5.206 ± 0.046	1.37SerMet: 1.37 ± 0.023	1.254SerAsn: 1.254 ± 0.026	2.904SerPro: 2.904 ± 0.038	1.469SerGln: 1.469 ± 0.023	3.627SerArg: 3.627 ± 0.046	2.797SerSer: 2.797 ± 0.043	2.738SerThr: 2.738 ± 0.042	3.843SerVal: 3.843 ± 0.047	0.841SerTrp: 0.841 ± 0.019	1.272SerTyr: 1.272 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	6.06ThrAla: 6.06 ± 0.058	0.446ThrCys: 0.446 ± 0.016	2.591ThrAsp: 2.591 ± 0.04	2.379ThrGlu: 2.379 ± 0.031	2.031ThrPhe: 2.031 ± 0.031	4.849ThrGly: 4.849 ± 0.056	0.987ThrHis: 0.987 ± 0.022	2.795ThrIle: 2.795 ± 0.04	1.49ThrLys: 1.49 ± 0.033	5.684ThrLeu: 5.684 ± 0.062	1.264ThrMet: 1.264 ± 0.025	1.309ThrAsn: 1.309 ± 0.028	3.357ThrPro: 3.357 ± 0.045	1.307ThrGln: 1.307 ± 0.029	3.33ThrArg: 3.33 ± 0.043	2.656ThrSer: 2.656 ± 0.043	3.025ThrThr: 3.025 ± 0.051	4.501ThrVal: 4.501 ± 0.051	0.766ThrTrp: 0.766 ± 0.018	1.257ThrTyr: 1.257 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	9.841ValAla: 9.841 ± 0.078	0.697ValCys: 0.697 ± 0.019	4.361ValAsp: 4.361 ± 0.054	4.464ValGlu: 4.464 ± 0.051	2.649ValPhe: 2.649 ± 0.037	6.338ValGly: 6.338 ± 0.061	1.473ValHis: 1.473 ± 0.03	3.621ValIle: 3.621 ± 0.043	2.412ValLys: 2.412 ± 0.041	7.746ValLeu: 7.746 ± 0.067	1.977ValMet: 1.977 ± 0.029	1.958ValAsn: 1.958 ± 0.034	4.254ValPro: 4.254 ± 0.049	2.1ValGln: 2.1 ± 0.03	5.519ValArg: 5.519 ± 0.056	4.109ValSer: 4.109 ± 0.049	4.25ValThr: 4.25 ± 0.048	6.648ValVal: 6.648 ± 0.071	1.02ValTrp: 1.02 ± 0.021	1.489ValTyr: 1.489 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.414TrpAla: 1.414 ± 0.027	0.161TrpCys: 0.161 ± 0.009	0.694TrpAsp: 0.694 ± 0.02	0.596TrpGlu: 0.596 ± 0.019	0.566TrpPhe: 0.566 ± 0.018	1.093TrpGly: 1.093 ± 0.022	0.439TrpHis: 0.439 ± 0.013	0.725TrpIle: 0.725 ± 0.019	0.58TrpLys: 0.58 ± 0.017	1.886TrpLeu: 1.886 ± 0.035	0.406TrpMet: 0.406 ± 0.014	0.5TrpAsn: 0.5 ± 0.015	0.874TrpPro: 0.874 ± 0.019	0.642TrpGln: 0.642 ± 0.015	1.504TrpArg: 1.504 ± 0.029	0.893TrpSer: 0.893 ± 0.021	0.919TrpThr: 0.919 ± 0.021	0.921TrpVal: 0.921 ± 0.02	0.271TrpTrp: 0.271 ± 0.011	0.329TrpTyr: 0.329 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.591TyrAla: 2.591 ± 0.038	0.282TyrCys: 0.282 ± 0.013	1.49TyrAsp: 1.49 ± 0.03	1.207TyrGlu: 1.207 ± 0.024	0.934TyrPhe: 0.934 ± 0.021	2.2TyrGly: 2.2 ± 0.032	0.485TyrHis: 0.485 ± 0.017	0.867TyrIle: 0.867 ± 0.021	0.655TyrLys: 0.655 ± 0.021	2.269TyrLeu: 2.269 ± 0.033	0.462TyrMet: 0.462 ± 0.016	0.613TyrAsn: 0.613 ± 0.017	1.116TyrPro: 1.116 ± 0.026	0.696TyrGln: 0.696 ± 0.019	1.847TyrArg: 1.847 ± 0.033	1.14TyrSer: 1.14 ± 0.026	1.091TyrThr: 1.091 ± 0.025	1.724TyrVal: 1.724 ± 0.026	0.422TyrTrp: 0.422 ± 0.014	0.587TyrTyr: 0.587 ± 0.019	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 7065 proteins (2124564 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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