Streptomyces sp. SID13588

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8348 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B2WIU3|A0A6B2WIU3_9ACTN DUF192 domain-containing protein OS=Streptomyces sp. SID13588 OX=2706051 GN=G3I68_23540 PE=4 SV=1
MM1 pKa = 6.97ITIVCHH7 pKa = 6.2CGDD10 pKa = 4.39NIGLPSGTEE19 pKa = 3.71TDD21 pKa = 3.34QDD23 pKa = 3.76DD24 pKa = 3.4GRR26 pKa = 11.84IYY28 pKa = 10.84HH29 pKa = 6.9GDD31 pKa = 3.32DD32 pKa = 3.49DD33 pKa = 4.92GRR35 pKa = 11.84PTACPLDD42 pKa = 4.01DD43 pKa = 4.41EE44 pKa = 5.06GEE46 pKa = 4.02IRR48 pKa = 11.84AA49 pKa = 4.31

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B2WK88|A0A6B2WK88_9ACTN Uncharacterized protein OS=Streptomyces sp. SID13588 OX=2706051 GN=G3I68_29905 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILATRR35 pKa = 11.84RR36 pKa = 11.84VKK38 pKa = 10.66GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8348

0

8348

2699635

29

7615

323.4

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.583 ± 0.045

0.811 ± 0.008

5.938 ± 0.022

5.227 ± 0.026

2.765 ± 0.017

9.453 ± 0.029

2.302 ± 0.013

3.436 ± 0.019

2.126 ± 0.022

10.36 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.756 ± 0.01

1.948 ± 0.019

6.085 ± 0.023

2.942 ± 0.018

7.652 ± 0.029

5.34 ± 0.021

6.372 ± 0.033

8.244 ± 0.027

1.548 ± 0.011

2.114 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski