Amino acid dipepetide frequency for Streptomyces sp. SID13588

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.947AlaAla: 21.947 ± 0.144
1.099AlaCys: 1.099 ± 0.023
8.339AlaAsp: 8.339 ± 0.064
7.95AlaGlu: 7.95 ± 0.074
3.615AlaPhe: 3.615 ± 0.045
13.408AlaGly: 13.408 ± 0.079
2.786AlaHis: 2.786 ± 0.037
3.849AlaIle: 3.849 ± 0.039
2.947AlaLys: 2.947 ± 0.047
14.465AlaLeu: 14.465 ± 0.097
2.586AlaMet: 2.586 ± 0.033
2.184AlaAsn: 2.184 ± 0.033
6.984AlaPro: 6.984 ± 0.065
3.932AlaGln: 3.932 ± 0.039
9.404AlaArg: 9.404 ± 0.08
6.037AlaSer: 6.037 ± 0.052
7.463AlaThr: 7.463 ± 0.057
12.259AlaVal: 12.259 ± 0.087
1.867AlaTrp: 1.867 ± 0.024
2.702AlaTyr: 2.702 ± 0.033
0.0AlaXaa: 0.0 ± 0.0
Cys
1.071CysAla: 1.071 ± 0.022
0.09CysCys: 0.09 ± 0.006
0.506CysAsp: 0.506 ± 0.016
0.399CysGlu: 0.399 ± 0.013
0.228CysPhe: 0.228 ± 0.009
0.986CysGly: 0.986 ± 0.023
0.206CysHis: 0.206 ± 0.008
0.176CysIle: 0.176 ± 0.009
0.123CysLys: 0.123 ± 0.007
0.772CysLeu: 0.772 ± 0.018
0.133CysMet: 0.133 ± 0.006
0.15CysAsn: 0.15 ± 0.009
0.504CysPro: 0.504 ± 0.014
0.182CysGln: 0.182 ± 0.008
0.62CysArg: 0.62 ± 0.016
0.482CysSer: 0.482 ± 0.015
0.527CysThr: 0.527 ± 0.013
0.659CysVal: 0.659 ± 0.016
0.133CysTrp: 0.133 ± 0.008
0.164CysTyr: 0.164 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.681AspAla: 7.681 ± 0.068
0.457AspCys: 0.457 ± 0.014
3.419AspAsp: 3.419 ± 0.042
3.569AspGlu: 3.569 ± 0.045
1.622AspPhe: 1.622 ± 0.026
6.244AspGly: 6.244 ± 0.052
1.419AspHis: 1.419 ± 0.027
2.008AspIle: 2.008 ± 0.03
1.135AspLys: 1.135 ± 0.025
6.274AspLeu: 6.274 ± 0.057
0.787AspMet: 0.787 ± 0.018
1.038AspAsn: 1.038 ± 0.021
4.615AspPro: 4.615 ± 0.048
1.68AspGln: 1.68 ± 0.03
4.854AspArg: 4.854 ± 0.049
2.678AspSer: 2.678 ± 0.031
3.297AspThr: 3.297 ± 0.037
4.479AspVal: 4.479 ± 0.037
1.011AspTrp: 1.011 ± 0.02
1.115AspTyr: 1.115 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
6.626GluAla: 6.626 ± 0.064
0.355GluCys: 0.355 ± 0.012
2.519GluAsp: 2.519 ± 0.036
2.957GluGlu: 2.957 ± 0.044
1.439GluPhe: 1.439 ± 0.023
3.76GluGly: 3.76 ± 0.042
1.517GluHis: 1.517 ± 0.025
2.208GluIle: 2.208 ± 0.028
1.194GluLys: 1.194 ± 0.023
6.573GluLeu: 6.573 ± 0.06
0.864GluMet: 0.864 ± 0.018
0.998GluAsn: 0.998 ± 0.022
3.177GluPro: 3.177 ± 0.038
2.231GluGln: 2.231 ± 0.034
4.886GluArg: 4.886 ± 0.051
2.469GluSer: 2.469 ± 0.033
2.657GluThr: 2.657 ± 0.031
4.03GluVal: 4.03 ± 0.044
0.739GluTrp: 0.739 ± 0.019
1.072GluTyr: 1.072 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
3.788PheAla: 3.788 ± 0.044
0.284PheCys: 0.284 ± 0.011
1.975PheAsp: 1.975 ± 0.03
1.331PheGlu: 1.331 ± 0.022
0.87PhePhe: 0.87 ± 0.025
3.087PheGly: 3.087 ± 0.04
0.655PheHis: 0.655 ± 0.015
0.826PheIle: 0.826 ± 0.019
0.516PheLys: 0.516 ± 0.015
2.535PheLeu: 2.535 ± 0.029
0.403PheMet: 0.403 ± 0.011
0.623PheAsn: 0.623 ± 0.016
1.412PhePro: 1.412 ± 0.023
0.726PheGln: 0.726 ± 0.018
1.784PheArg: 1.784 ± 0.028
1.579PheSer: 1.579 ± 0.024
2.178PheThr: 2.178 ± 0.029
2.098PheVal: 2.098 ± 0.034
0.412PheTrp: 0.412 ± 0.013
0.57PheTyr: 0.57 ± 0.017
0.0PheXaa: 0.0 ± 0.0
Gly
10.939GlyAla: 10.939 ± 0.071
0.88GlyCys: 0.88 ± 0.02
5.092GlyAsp: 5.092 ± 0.044
4.628GlyGlu: 4.628 ± 0.043
2.934GlyPhe: 2.934 ± 0.032
8.843GlyGly: 8.843 ± 0.078
2.301GlyHis: 2.301 ± 0.03
3.703GlyIle: 3.703 ± 0.041
2.416GlyLys: 2.416 ± 0.037
9.442GlyLeu: 9.442 ± 0.071
1.991GlyMet: 1.991 ± 0.03
1.928GlyAsn: 1.928 ± 0.038
5.177GlyPro: 5.177 ± 0.051
2.919GlyGln: 2.919 ± 0.038
7.422GlyArg: 7.422 ± 0.058
5.85GlySer: 5.85 ± 0.055
6.816GlyThr: 6.816 ± 0.06
7.096GlyVal: 7.096 ± 0.063
1.778GlyTrp: 1.778 ± 0.027
2.37GlyTyr: 2.37 ± 0.035
0.0GlyXaa: 0.0 ± 0.0
His
2.707HisAla: 2.707 ± 0.035
0.211HisCys: 0.211 ± 0.009
1.348HisAsp: 1.348 ± 0.023
1.149HisGlu: 1.149 ± 0.022
0.681HisPhe: 0.681 ± 0.018
2.377HisGly: 2.377 ± 0.032
0.705HisHis: 0.705 ± 0.019
0.747HisIle: 0.747 ± 0.016
0.358HisLys: 0.358 ± 0.013
2.487HisLeu: 2.487 ± 0.035
0.32HisMet: 0.32 ± 0.01
0.402HisAsn: 0.402 ± 0.011
1.8HisPro: 1.8 ± 0.025
0.731HisGln: 0.731 ± 0.017
2.036HisArg: 2.036 ± 0.027
1.124HisSer: 1.124 ± 0.02
1.381HisThr: 1.381 ± 0.026
1.582HisVal: 1.582 ± 0.025
0.364HisTrp: 0.364 ± 0.011
0.506HisTyr: 0.506 ± 0.015
0.0HisXaa: 0.0 ± 0.0
Ile
5.232IleAla: 5.232 ± 0.045
0.311IleCys: 0.311 ± 0.01
2.386IleAsp: 2.386 ± 0.033
1.986IleGlu: 1.986 ± 0.03
0.783IlePhe: 0.783 ± 0.017
3.85IleGly: 3.85 ± 0.041
0.685IleHis: 0.685 ± 0.015
1.048IleIle: 1.048 ± 0.023
0.79IleLys: 0.79 ± 0.019
2.616IleLeu: 2.616 ± 0.032
0.476IleMet: 0.476 ± 0.014
0.825IleAsn: 0.825 ± 0.019
1.938IlePro: 1.938 ± 0.028
0.818IleGln: 0.818 ± 0.019
2.327IleArg: 2.327 ± 0.029
1.911IleSer: 1.911 ± 0.029
2.518IleThr: 2.518 ± 0.033
2.884IleVal: 2.884 ± 0.037
0.393IleTrp: 0.393 ± 0.013
0.581IleTyr: 0.581 ± 0.014
0.0IleXaa: 0.0 ± 0.0
Lys
2.907LysAla: 2.907 ± 0.046
0.133LysCys: 0.133 ± 0.008
1.306LysAsp: 1.306 ± 0.028
1.06LysGlu: 1.06 ± 0.022
0.476LysPhe: 0.476 ± 0.016
1.756LysGly: 1.756 ± 0.032
0.44LysHis: 0.44 ± 0.013
0.887LysIle: 0.887 ± 0.018
0.738LysLys: 0.738 ± 0.026
2.056LysLeu: 2.056 ± 0.032
0.383LysMet: 0.383 ± 0.013
0.569LysAsn: 0.569 ± 0.014
1.365LysPro: 1.365 ± 0.026
0.685LysGln: 0.685 ± 0.018
1.385LysArg: 1.385 ± 0.024
1.206LysSer: 1.206 ± 0.023
1.273LysThr: 1.273 ± 0.024
1.883LysVal: 1.883 ± 0.035
0.263LysTrp: 0.263 ± 0.01
0.485LysTyr: 0.485 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
15.052LeuAla: 15.052 ± 0.103
0.847LeuCys: 0.847 ± 0.02
6.663LeuAsp: 6.663 ± 0.056
4.518LeuGlu: 4.518 ± 0.045
2.636LeuPhe: 2.636 ± 0.038
9.309LeuGly: 9.309 ± 0.07
2.393LeuHis: 2.393 ± 0.034
3.564LeuIle: 3.564 ± 0.038
1.947LeuLys: 1.947 ± 0.031
11.456LeuLeu: 11.456 ± 0.112
1.687LeuMet: 1.687 ± 0.027
1.869LeuAsn: 1.869 ± 0.027
6.59LeuPro: 6.59 ± 0.064
2.42LeuGln: 2.42 ± 0.031
8.518LeuArg: 8.518 ± 0.071
5.563LeuSer: 5.563 ± 0.044
6.838LeuThr: 6.838 ± 0.052
8.541LeuVal: 8.541 ± 0.061
1.325LeuTrp: 1.325 ± 0.018
1.867LeuTyr: 1.867 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
2.291MetAla: 2.291 ± 0.033
0.139MetCys: 0.139 ± 0.006
0.896MetAsp: 0.896 ± 0.018
0.747MetGlu: 0.747 ± 0.017
0.464MetPhe: 0.464 ± 0.013
1.318MetGly: 1.318 ± 0.024
0.356MetHis: 0.356 ± 0.012
0.688MetIle: 0.688 ± 0.016
0.418MetLys: 0.418 ± 0.014
1.741MetLeu: 1.741 ± 0.027
0.306MetMet: 0.306 ± 0.011
0.45MetAsn: 0.45 ± 0.013
1.153MetPro: 1.153 ± 0.024
0.459MetGln: 0.459 ± 0.013
1.419MetArg: 1.419 ± 0.025
1.319MetSer: 1.319 ± 0.023
1.545MetThr: 1.545 ± 0.024
1.299MetVal: 1.299 ± 0.023
0.223MetTrp: 0.223 ± 0.01
0.327MetTyr: 0.327 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
2.398AsnAla: 2.398 ± 0.039
0.166AsnCys: 0.166 ± 0.008
1.062AsnAsp: 1.062 ± 0.021
0.86AsnGlu: 0.86 ± 0.02
0.492AsnPhe: 0.492 ± 0.016
2.213AsnGly: 2.213 ± 0.036
0.447AsnHis: 0.447 ± 0.012
0.729AsnIle: 0.729 ± 0.019
0.431AsnLys: 0.431 ± 0.014
1.808AsnLeu: 1.808 ± 0.032
0.31AsnMet: 0.31 ± 0.01
0.499AsnAsn: 0.499 ± 0.016
1.454AsnPro: 1.454 ± 0.027
0.595AsnGln: 0.595 ± 0.016
1.365AsnArg: 1.365 ± 0.024
1.083AsnSer: 1.083 ± 0.021
1.297AsnThr: 1.297 ± 0.025
1.486AsnVal: 1.486 ± 0.026
0.333AsnTrp: 0.333 ± 0.013
0.45AsnTyr: 0.45 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
9.006ProAla: 9.006 ± 0.069
0.35ProCys: 0.35 ± 0.012
4.456ProAsp: 4.456 ± 0.047
3.895ProGlu: 3.895 ± 0.046
1.545ProPhe: 1.545 ± 0.027
6.642ProGly: 6.642 ± 0.056
1.351ProHis: 1.351 ± 0.023
1.433ProIle: 1.433 ± 0.025
1.213ProLys: 1.213 ± 0.024
5.357ProLeu: 5.357 ± 0.045
1.008ProMet: 1.008 ± 0.021
1.028ProAsn: 1.028 ± 0.02
3.567ProPro: 3.567 ± 0.049
1.888ProGln: 1.888 ± 0.029
3.71ProArg: 3.71 ± 0.041
3.291ProSer: 3.291 ± 0.042
3.347ProThr: 3.347 ± 0.042
5.465ProVal: 5.465 ± 0.049
0.922ProTrp: 0.922 ± 0.021
1.375ProTyr: 1.375 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.879GlnAla: 3.879 ± 0.048
0.179GlnCys: 0.179 ± 0.008
1.586GlnAsp: 1.586 ± 0.026
1.432GlnGlu: 1.432 ± 0.022
0.743GlnPhe: 0.743 ± 0.018
2.38GlnGly: 2.38 ± 0.031
0.715GlnHis: 0.715 ± 0.018
1.2GlnIle: 1.2 ± 0.024
0.597GlnLys: 0.597 ± 0.018
3.34GlnLeu: 3.34 ± 0.043
0.489GlnMet: 0.489 ± 0.013
0.59GlnAsn: 0.59 ± 0.018
1.802GlnPro: 1.802 ± 0.031
1.392GlnGln: 1.392 ± 0.033
2.494GlnArg: 2.494 ± 0.033
1.421GlnSer: 1.421 ± 0.025
1.439GlnThr: 1.439 ± 0.024
2.514GlnVal: 2.514 ± 0.034
0.547GlnTrp: 0.547 ± 0.017
0.678GlnTyr: 0.678 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
9.167ArgAla: 9.167 ± 0.078
0.597ArgCys: 0.597 ± 0.016
4.128ArgAsp: 4.128 ± 0.039
4.164ArgGlu: 4.164 ± 0.047
2.26ArgPhe: 2.26 ± 0.032
5.387ArgGly: 5.387 ± 0.048
1.976ArgHis: 1.976 ± 0.03
3.366ArgIle: 3.366 ± 0.039
1.627ArgLys: 1.627 ± 0.025
8.461ArgLeu: 8.461 ± 0.078
1.722ArgMet: 1.722 ± 0.024
1.444ArgAsn: 1.444 ± 0.021
4.727ArgPro: 4.727 ± 0.045
2.333ArgGln: 2.333 ± 0.028
7.088ArgArg: 7.088 ± 0.07
4.231ArgSer: 4.231 ± 0.045
5.29ArgThr: 5.29 ± 0.051
5.361ArgVal: 5.361 ± 0.052
1.402ArgTrp: 1.402 ± 0.024
1.786ArgTyr: 1.786 ± 0.023
0.0ArgXaa: 0.0 ± 0.0
Ser
7.078SerAla: 7.078 ± 0.05
0.463SerCys: 0.463 ± 0.015
2.902SerAsp: 2.902 ± 0.036
2.348SerGlu: 2.348 ± 0.03
1.595SerPhe: 1.595 ± 0.028
6.286SerGly: 6.286 ± 0.066
1.094SerHis: 1.094 ± 0.019
1.664SerIle: 1.664 ± 0.031
1.075SerLys: 1.075 ± 0.023
4.928SerLeu: 4.928 ± 0.053
1.135SerMet: 1.135 ± 0.021
1.032SerAsn: 1.032 ± 0.024
3.257SerPro: 3.257 ± 0.036
1.424SerGln: 1.424 ± 0.024
3.733SerArg: 3.733 ± 0.04
3.193SerSer: 3.193 ± 0.048
3.417SerThr: 3.417 ± 0.044
4.454SerVal: 4.454 ± 0.052
0.988SerTrp: 0.988 ± 0.02
1.335SerTyr: 1.335 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
9.402ThrAla: 9.402 ± 0.056
0.436ThrCys: 0.436 ± 0.013
3.76ThrAsp: 3.76 ± 0.045
3.069ThrGlu: 3.069 ± 0.037
1.728ThrPhe: 1.728 ± 0.028
7.116ThrGly: 7.116 ± 0.062
1.255ThrHis: 1.255 ± 0.02
1.961ThrIle: 1.961 ± 0.028
1.25ThrLys: 1.25 ± 0.026
5.79ThrLeu: 5.79 ± 0.044
0.976ThrMet: 0.976 ± 0.021
1.124ThrAsn: 1.124 ± 0.023
4.193ThrPro: 4.193 ± 0.05
1.472ThrGln: 1.472 ± 0.028
3.79ThrArg: 3.79 ± 0.039
3.481ThrSer: 3.481 ± 0.05
4.339ThrThr: 4.339 ± 0.068
6.243ThrVal: 6.243 ± 0.057
0.95ThrTrp: 0.95 ± 0.022
1.386ThrTyr: 1.386 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
10.295ValAla: 10.295 ± 0.07
0.752ValCys: 0.752 ± 0.017
4.673ValAsp: 4.673 ± 0.045
4.349ValGlu: 4.349 ± 0.048
2.363ValPhe: 2.363 ± 0.035
6.276ValGly: 6.276 ± 0.059
1.937ValHis: 1.937 ± 0.027
3.132ValIle: 3.132 ± 0.041
1.729ValLys: 1.729 ± 0.028
9.499ValLeu: 9.499 ± 0.067
1.422ValMet: 1.422 ± 0.028
1.801ValAsn: 1.801 ± 0.028
5.203ValPro: 5.203 ± 0.048
2.19ValGln: 2.19 ± 0.028
6.605ValArg: 6.605 ± 0.049
4.363ValSer: 4.363 ± 0.044
5.674ValThr: 5.674 ± 0.061
7.526ValVal: 7.526 ± 0.063
1.114ValTrp: 1.114 ± 0.022
1.536ValTyr: 1.536 ± 0.024
0.0ValXaa: 0.0 ± 0.0
Trp
1.628TrpAla: 1.628 ± 0.023
0.172TrpCys: 0.172 ± 0.008
0.851TrpAsp: 0.851 ± 0.019
0.698TrpGlu: 0.698 ± 0.017
0.517TrpPhe: 0.517 ± 0.015
1.069TrpGly: 1.069 ± 0.02
0.393TrpHis: 0.393 ± 0.013
0.61TrpIle: 0.61 ± 0.014
0.384TrpLys: 0.384 ± 0.013
1.785TrpLeu: 1.785 ± 0.033
0.306TrpMet: 0.306 ± 0.012
0.44TrpAsn: 0.44 ± 0.015
0.792TrpPro: 0.792 ± 0.016
0.672TrpGln: 0.672 ± 0.016
1.258TrpArg: 1.258 ± 0.025
1.068TrpSer: 1.068 ± 0.022
1.138TrpThr: 1.138 ± 0.025
0.991TrpVal: 0.991 ± 0.021
0.338TrpTrp: 0.338 ± 0.011
0.37TrpTyr: 0.37 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.734TyrAla: 2.734 ± 0.034
0.189TyrCys: 0.189 ± 0.007
1.515TyrAsp: 1.515 ± 0.04
1.16TyrGlu: 1.16 ± 0.02
0.663TyrPhe: 0.663 ± 0.016
2.219TyrGly: 2.219 ± 0.03
0.386TyrHis: 0.386 ± 0.012
0.558TyrIle: 0.558 ± 0.016
0.366TyrLys: 0.366 ± 0.011
2.217TyrLeu: 2.217 ± 0.031
0.253TyrMet: 0.253 ± 0.01
0.485TyrAsn: 0.485 ± 0.015
1.14TyrPro: 1.14 ± 0.022
0.667TyrGln: 0.667 ± 0.014
1.82TyrArg: 1.82 ± 0.028
1.051TyrSer: 1.051 ± 0.022
1.289TyrThr: 1.289 ± 0.034
1.588TyrVal: 1.588 ± 0.029
0.378TyrTrp: 0.378 ± 0.011
0.463TyrTyr: 0.463 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8348 proteins (2699635 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski