Amino acid dipepetide frequency for Flavobacteriaceae bacterium R38

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.829AlaAla: 3.829 ± 0.08	0.512AlaCys: 0.512 ± 0.024	3.095AlaAsp: 3.095 ± 0.051	3.593AlaGlu: 3.593 ± 0.058	3.433AlaPhe: 3.433 ± 0.049	3.759AlaGly: 3.759 ± 0.068	1.034AlaHis: 1.034 ± 0.033	5.608AlaIle: 5.608 ± 0.082	4.429AlaLys: 4.429 ± 0.067	6.004AlaLeu: 6.004 ± 0.093	1.305AlaMet: 1.305 ± 0.038	3.36AlaAsn: 3.36 ± 0.061	1.838AlaPro: 1.838 ± 0.051	2.087AlaGln: 2.087 ± 0.042	1.994AlaArg: 1.994 ± 0.039	4.02AlaSer: 4.02 ± 0.062	3.379AlaThr: 3.379 ± 0.081	3.81AlaVal: 3.81 ± 0.063	0.572AlaTrp: 0.572 ± 0.026	2.351AlaTyr: 2.351 ± 0.049	0.0AlaXaa: 0.0 ± 0.0
Cys	0.377CysAla: 0.377 ± 0.021	0.092CysCys: 0.092 ± 0.011	0.394CysAsp: 0.394 ± 0.021	0.409CysGlu: 0.409 ± 0.019	0.423CysPhe: 0.423 ± 0.019	0.548CysGly: 0.548 ± 0.025	0.162CysHis: 0.162 ± 0.011	0.591CysIle: 0.591 ± 0.021	0.417CysLys: 0.417 ± 0.021	0.606CysLeu: 0.606 ± 0.022	0.132CysMet: 0.132 ± 0.01	0.434CysAsn: 0.434 ± 0.02	0.344CysPro: 0.344 ± 0.022	0.201CysGln: 0.201 ± 0.013	0.18CysArg: 0.18 ± 0.011	0.513CysSer: 0.513 ± 0.023	0.446CysThr: 0.446 ± 0.027	0.373CysVal: 0.373 ± 0.019	0.052CysTrp: 0.052 ± 0.007	0.266CysTyr: 0.266 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.56AspAla: 3.56 ± 0.058	0.369AspCys: 0.369 ± 0.018	3.153AspAsp: 3.153 ± 0.07	3.947AspGlu: 3.947 ± 0.064	3.748AspPhe: 3.748 ± 0.067	3.572AspGly: 3.572 ± 0.084	1.038AspHis: 1.038 ± 0.03	4.734AspIle: 4.734 ± 0.067	4.142AspLys: 4.142 ± 0.068	5.042AspLeu: 5.042 ± 0.069	0.903AspMet: 0.903 ± 0.031	3.292AspAsn: 3.292 ± 0.064	1.846AspPro: 1.846 ± 0.055	1.678AspGln: 1.678 ± 0.054	1.989AspArg: 1.989 ± 0.04	3.141AspSer: 3.141 ± 0.063	2.953AspThr: 2.953 ± 0.061	3.509AspVal: 3.509 ± 0.065	0.708AspTrp: 0.708 ± 0.028	2.699AspTyr: 2.699 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.365GluAla: 4.365 ± 0.081	0.324GluCys: 0.324 ± 0.017	3.656GluAsp: 3.656 ± 0.049	5.532GluGlu: 5.532 ± 0.088	2.892GluPhe: 2.892 ± 0.049	3.744GluGly: 3.744 ± 0.061	1.151GluHis: 1.151 ± 0.034	5.932GluIle: 5.932 ± 0.078	6.468GluLys: 6.468 ± 0.105	6.551GluLeu: 6.551 ± 0.083	1.439GluMet: 1.439 ± 0.036	4.833GluAsn: 4.833 ± 0.067	1.478GluPro: 1.478 ± 0.035	2.218GluGln: 2.218 ± 0.041	2.639GluArg: 2.639 ± 0.058	3.387GluSer: 3.387 ± 0.063	3.514GluThr: 3.514 ± 0.055	4.497GluVal: 4.497 ± 0.062	0.61GluTrp: 0.61 ± 0.025	2.535GluTyr: 2.535 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.739PheAla: 2.739 ± 0.052	0.397PheCys: 0.397 ± 0.018	3.384PheAsp: 3.384 ± 0.062	3.481PheGlu: 3.481 ± 0.062	3.102PhePhe: 3.102 ± 0.066	3.412PheGly: 3.412 ± 0.063	0.84PheHis: 0.84 ± 0.028	4.45PheIle: 4.45 ± 0.065	4.009PheLys: 4.009 ± 0.065	5.118PheLeu: 5.118 ± 0.086	1.034PheMet: 1.034 ± 0.03	3.654PheAsn: 3.654 ± 0.068	1.765PhePro: 1.765 ± 0.043	1.572PheGln: 1.572 ± 0.037	1.748PheArg: 1.748 ± 0.037	4.218PheSer: 4.218 ± 0.068	3.206PheThr: 3.206 ± 0.064	2.799PheVal: 2.799 ± 0.045	0.579PheTrp: 0.579 ± 0.026	2.313PheTyr: 2.313 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	3.967GlyAla: 3.967 ± 0.067	0.59GlyCys: 0.59 ± 0.037	3.454GlyAsp: 3.454 ± 0.067	3.603GlyGlu: 3.603 ± 0.059	3.628GlyPhe: 3.628 ± 0.06	4.703GlyGly: 4.703 ± 0.085	1.097GlyHis: 1.097 ± 0.039	5.609GlyIle: 5.609 ± 0.072	4.8GlyLys: 4.8 ± 0.081	5.602GlyLeu: 5.602 ± 0.077	1.435GlyMet: 1.435 ± 0.043	3.844GlyAsn: 3.844 ± 0.076	1.367GlyPro: 1.367 ± 0.042	1.787GlyGln: 1.787 ± 0.039	2.16GlyArg: 2.16 ± 0.046	3.811GlySer: 3.811 ± 0.085	3.795GlyThr: 3.795 ± 0.079	4.185GlyVal: 4.185 ± 0.076	0.686GlyTrp: 0.686 ± 0.024	2.685GlyTyr: 2.685 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	0.894HisAla: 0.894 ± 0.025	0.17HisCys: 0.17 ± 0.013	0.798HisAsp: 0.798 ± 0.028	1.005HisGlu: 1.005 ± 0.028	1.156HisPhe: 1.156 ± 0.032	0.973HisGly: 0.973 ± 0.032	0.535HisHis: 0.535 ± 0.023	1.467HisIle: 1.467 ± 0.035	1.322HisLys: 1.322 ± 0.036	1.889HisLeu: 1.889 ± 0.047	0.304HisMet: 0.304 ± 0.016	0.883HisAsn: 0.883 ± 0.026	0.894HisPro: 0.894 ± 0.032	0.755HisGln: 0.755 ± 0.026	0.692HisArg: 0.692 ± 0.024	1.046HisSer: 1.046 ± 0.031	0.903HisThr: 0.903 ± 0.032	0.896HisVal: 0.896 ± 0.03	0.21HisTrp: 0.21 ± 0.015	0.818HisTyr: 0.818 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.821IleAla: 5.821 ± 0.076	0.596IleCys: 0.596 ± 0.02	5.297IleAsp: 5.297 ± 0.078	5.94IleGlu: 5.94 ± 0.092	3.914IlePhe: 3.914 ± 0.065	5.344IleGly: 5.344 ± 0.072	1.487IleHis: 1.487 ± 0.037	6.865IleIle: 6.865 ± 0.104	6.375IleLys: 6.375 ± 0.094	7.517IleLeu: 7.517 ± 0.103	1.336IleMet: 1.336 ± 0.038	5.27IleAsn: 5.27 ± 0.085	3.35IlePro: 3.35 ± 0.057	2.678IleGln: 2.678 ± 0.047	2.922IleArg: 2.922 ± 0.052	6.12IleSer: 6.12 ± 0.083	5.14IleThr: 5.14 ± 0.104	5.009IleVal: 5.009 ± 0.068	0.747IleTrp: 0.747 ± 0.026	2.939IleTyr: 2.939 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.868LysAla: 4.868 ± 0.087	0.309LysCys: 0.309 ± 0.017	4.401LysAsp: 4.401 ± 0.074	7.017LysGlu: 7.017 ± 0.105	2.779LysPhe: 2.779 ± 0.05	4.581LysGly: 4.581 ± 0.069	1.391LysHis: 1.391 ± 0.038	6.655LysIle: 6.655 ± 0.102	8.027LysLys: 8.027 ± 0.121	6.768LysLeu: 6.768 ± 0.086	1.849LysMet: 1.849 ± 0.04	5.751LysAsn: 5.751 ± 0.091	2.203LysPro: 2.203 ± 0.046	2.63LysGln: 2.63 ± 0.053	3.177LysArg: 3.177 ± 0.065	4.699LysSer: 4.699 ± 0.087	4.634LysThr: 4.634 ± 0.081	4.549LysVal: 4.549 ± 0.074	0.843LysTrp: 0.843 ± 0.031	3.088LysTyr: 3.088 ± 0.063	0.0LysXaa: 0.0 ± 0.0
Leu	5.41LeuAla: 5.41 ± 0.076	0.633LeuCys: 0.633 ± 0.026	5.065LeuAsp: 5.065 ± 0.075	6.158LeuGlu: 6.158 ± 0.077	5.179LeuPhe: 5.179 ± 0.077	5.7LeuGly: 5.7 ± 0.074	1.539LeuHis: 1.539 ± 0.038	8.062LeuIle: 8.062 ± 0.112	8.293LeuLys: 8.293 ± 0.114	8.821LeuLeu: 8.821 ± 0.114	1.941LeuMet: 1.941 ± 0.045	6.051LeuAsn: 6.051 ± 0.071	3.434LeuPro: 3.434 ± 0.059	2.977LeuGln: 2.977 ± 0.061	3.367LeuArg: 3.367 ± 0.053	6.911LeuSer: 6.911 ± 0.079	5.018LeuThr: 5.018 ± 0.076	5.03LeuVal: 5.03 ± 0.076	0.889LeuTrp: 0.889 ± 0.03	3.121LeuTyr: 3.121 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	1.259MetAla: 1.259 ± 0.035	0.124MetCys: 0.124 ± 0.011	0.951MetAsp: 0.951 ± 0.033	1.254MetGlu: 1.254 ± 0.036	0.8MetPhe: 0.8 ± 0.029	1.216MetGly: 1.216 ± 0.038	0.339MetHis: 0.339 ± 0.019	1.595MetIle: 1.595 ± 0.041	2.151MetLys: 2.151 ± 0.039	1.783MetLeu: 1.783 ± 0.047	0.512MetMet: 0.512 ± 0.022	1.285MetAsn: 1.285 ± 0.03	0.693MetPro: 0.693 ± 0.024	0.715MetGln: 0.715 ± 0.024	0.824MetArg: 0.824 ± 0.03	1.289MetSer: 1.289 ± 0.032	0.985MetThr: 0.985 ± 0.026	1.135MetVal: 1.135 ± 0.032	0.151MetTrp: 0.151 ± 0.012	0.623MetTyr: 0.623 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.784AsnAla: 3.784 ± 0.066	0.413AsnCys: 0.413 ± 0.021	3.654AsnAsp: 3.654 ± 0.063	4.056AsnGlu: 4.056 ± 0.056	3.132AsnPhe: 3.132 ± 0.061	4.153AsnGly: 4.153 ± 0.071	1.104AsnHis: 1.104 ± 0.036	5.112AsnIle: 5.112 ± 0.066	4.494AsnLys: 4.494 ± 0.073	5.532AsnLeu: 5.532 ± 0.078	1.188AsnMet: 1.188 ± 0.029	4.169AsnAsn: 4.169 ± 0.085	2.717AsnPro: 2.717 ± 0.051	2.231AsnGln: 2.231 ± 0.051	2.493AsnArg: 2.493 ± 0.05	3.876AsnSer: 3.876 ± 0.068	4.04AsnThr: 4.04 ± 0.071	3.52AsnVal: 3.52 ± 0.061	0.841AsnTrp: 0.841 ± 0.032	2.837AsnTyr: 2.837 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	1.767ProAla: 1.767 ± 0.054	0.188ProCys: 0.188 ± 0.013	2.239ProAsp: 2.239 ± 0.058	2.767ProGlu: 2.767 ± 0.052	1.931ProPhe: 1.931 ± 0.042	2.178ProGly: 2.178 ± 0.049	0.596ProHis: 0.596 ± 0.027	2.533ProIle: 2.533 ± 0.056	2.382ProLys: 2.382 ± 0.049	2.944ProLeu: 2.944 ± 0.045	0.607ProMet: 0.607 ± 0.027	2.1ProAsn: 2.1 ± 0.05	0.903ProPro: 0.903 ± 0.042	1.089ProGln: 1.089 ± 0.03	0.977ProArg: 0.977 ± 0.031	2.14ProSer: 2.14 ± 0.051	1.701ProThr: 1.701 ± 0.046	2.46ProVal: 2.46 ± 0.051	0.333ProTrp: 0.333 ± 0.021	1.332ProTyr: 1.332 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	1.849GlnAla: 1.849 ± 0.044	0.176GlnCys: 0.176 ± 0.013	1.542GlnAsp: 1.542 ± 0.04	2.386GlnGlu: 2.386 ± 0.047	1.679GlnPhe: 1.679 ± 0.039	1.784GlnGly: 1.784 ± 0.044	0.587GlnHis: 0.587 ± 0.025	2.606GlnIle: 2.606 ± 0.047	2.832GlnLys: 2.832 ± 0.058	3.474GlnLeu: 3.474 ± 0.06	0.72GlnMet: 0.72 ± 0.027	2.046GlnAsn: 2.046 ± 0.044	1.02GlnPro: 1.02 ± 0.031	1.386GlnGln: 1.386 ± 0.044	1.294GlnArg: 1.294 ± 0.037	1.862GlnSer: 1.862 ± 0.04	1.607GlnThr: 1.607 ± 0.04	1.893GlnVal: 1.893 ± 0.04	0.368GlnTrp: 0.368 ± 0.022	1.275GlnTyr: 1.275 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.064ArgAla: 2.064 ± 0.043	0.182ArgCys: 0.182 ± 0.011	1.911ArgAsp: 1.911 ± 0.046	2.452ArgGlu: 2.452 ± 0.055	2.125ArgPhe: 2.125 ± 0.045	2.072ArgGly: 2.072 ± 0.051	0.605ArgHis: 0.605 ± 0.025	3.24ArgIle: 3.24 ± 0.052	3.028ArgLys: 3.028 ± 0.058	3.453ArgLeu: 3.453 ± 0.065	0.831ArgMet: 0.831 ± 0.029	2.318ArgAsn: 2.318 ± 0.047	1.083ArgPro: 1.083 ± 0.032	1.126ArgGln: 1.126 ± 0.031	1.466ArgArg: 1.466 ± 0.044	2.091ArgSer: 2.091 ± 0.048	1.85ArgThr: 1.85 ± 0.041	2.231ArgVal: 2.231 ± 0.05	0.396ArgTrp: 0.396 ± 0.02	1.563ArgTyr: 1.563 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	3.267SerAla: 3.267 ± 0.06	0.629SerCys: 0.629 ± 0.027	3.644SerAsp: 3.644 ± 0.061	4.088SerGlu: 4.088 ± 0.059	4.19SerPhe: 4.19 ± 0.073	4.597SerGly: 4.597 ± 0.085	1.046SerHis: 1.046 ± 0.032	5.519SerIle: 5.519 ± 0.068	5.039SerLys: 5.039 ± 0.08	6.312SerLeu: 6.312 ± 0.074	1.159SerMet: 1.159 ± 0.033	3.84SerAsn: 3.84 ± 0.07	2.091SerPro: 2.091 ± 0.046	1.985SerGln: 1.985 ± 0.038	2.243SerArg: 2.243 ± 0.044	4.285SerSer: 4.285 ± 0.075	3.367SerThr: 3.367 ± 0.063	3.881SerVal: 3.881 ± 0.067	0.704SerTrp: 0.704 ± 0.029	2.832SerTyr: 2.832 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	3.673ThrAla: 3.673 ± 0.082	0.352ThrCys: 0.352 ± 0.022	3.081ThrAsp: 3.081 ± 0.077	3.202ThrGlu: 3.202 ± 0.053	3.09ThrPhe: 3.09 ± 0.07	3.928ThrGly: 3.928 ± 0.091	1.046ThrHis: 1.046 ± 0.03	5.233ThrIle: 5.233 ± 0.083	3.65ThrLys: 3.65 ± 0.067	5.292ThrLeu: 5.292 ± 0.075	0.818ThrMet: 0.818 ± 0.029	3.273ThrAsn: 3.273 ± 0.064	2.454ThrPro: 2.454 ± 0.067	1.73ThrGln: 1.73 ± 0.035	1.782ThrArg: 1.782 ± 0.043	3.862ThrSer: 3.862 ± 0.063	3.404ThrThr: 3.404 ± 0.073	3.737ThrVal: 3.737 ± 0.094	0.557ThrTrp: 0.557 ± 0.028	2.301ThrTyr: 2.301 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	3.735ValAla: 3.735 ± 0.07	0.481ValCys: 0.481 ± 0.024	3.431ValAsp: 3.431 ± 0.062	3.728ValGlu: 3.728 ± 0.059	3.532ValPhe: 3.532 ± 0.058	3.41ValGly: 3.41 ± 0.061	0.972ValHis: 0.972 ± 0.031	5.166ValIle: 5.166 ± 0.067	4.346ValLys: 4.346 ± 0.066	5.889ValLeu: 5.889 ± 0.082	1.245ValMet: 1.245 ± 0.035	3.74ValAsn: 3.74 ± 0.074	2.059ValPro: 2.059 ± 0.048	1.698ValGln: 1.698 ± 0.04	1.967ValArg: 1.967 ± 0.043	4.287ValSer: 4.287 ± 0.081	3.588ValThr: 3.588 ± 0.097	3.851ValVal: 3.851 ± 0.063	0.552ValTrp: 0.552 ± 0.024	2.357ValTyr: 2.357 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.538TrpAla: 0.538 ± 0.023	0.096TrpCys: 0.096 ± 0.009	0.585TrpAsp: 0.585 ± 0.026	0.711TrpGlu: 0.711 ± 0.029	0.59TrpPhe: 0.59 ± 0.024	0.657TrpGly: 0.657 ± 0.027	0.208TrpHis: 0.208 ± 0.013	0.829TrpIle: 0.829 ± 0.032	0.912TrpLys: 0.912 ± 0.033	0.953TrpLeu: 0.953 ± 0.033	0.271TrpMet: 0.271 ± 0.016	0.7TrpAsn: 0.7 ± 0.029	0.227TrpPro: 0.227 ± 0.016	0.327TrpGln: 0.327 ± 0.015	0.429TrpArg: 0.429 ± 0.022	0.614TrpSer: 0.614 ± 0.03	0.561TrpThr: 0.561 ± 0.026	0.631TrpVal: 0.631 ± 0.026	0.138TrpTrp: 0.138 ± 0.013	0.434TrpTyr: 0.434 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.248TyrAla: 2.248 ± 0.045	0.325TyrCys: 0.325 ± 0.015	2.292TyrAsp: 2.292 ± 0.053	2.435TyrGlu: 2.435 ± 0.056	2.498TyrPhe: 2.498 ± 0.05	2.476TyrGly: 2.476 ± 0.046	0.829TyrHis: 0.829 ± 0.026	2.864TyrIle: 2.864 ± 0.053	3.101TyrLys: 3.101 ± 0.054	4.049TyrLeu: 4.049 ± 0.066	0.653TyrMet: 0.653 ± 0.023	2.504TyrAsn: 2.504 ± 0.055	1.403TyrPro: 1.403 ± 0.035	1.518TyrGln: 1.518 ± 0.035	1.746TyrArg: 1.746 ± 0.044	2.544TyrSer: 2.544 ± 0.051	2.374TyrThr: 2.374 ± 0.05	2.037TyrVal: 2.037 ± 0.044	0.476TyrTrp: 0.476 ± 0.023	1.847TyrTyr: 1.847 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3541 proteins (1175463 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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