Mycobacterium phage Wildcat
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q19Y45|Q19Y45_9CAUD Uncharacterized protein OS=Mycobacterium phage Wildcat OX=373415 GN=15 PE=4 SV=1
MM1 pKa = 7.63 TDD3 pKa = 3.35 WDD5 pKa = 4.11 WVDD8 pKa = 3.15 QHH10 pKa = 8.24 IDD12 pKa = 3.33 TQLTIQEE19 pKa = 4.31 RR20 pKa = 11.84 GCYY23 pKa = 9.72 SNPFFEE29 pKa = 5.64 LKK31 pKa = 10.41 DD32 pKa = 3.82 DD33 pKa = 4.44 LVVTVDD39 pKa = 3.69 GARR42 pKa = 11.84 WTPTGWGEE50 pKa = 3.76 ATIGGEE56 pKa = 3.92 RR57 pKa = 11.84 YY58 pKa = 9.14 LHH60 pKa = 6.22 NWDD63 pKa = 4.39 EE64 pKa = 4.61 SPADD68 pKa = 3.61 VPDD71 pKa = 3.33 YY72 pKa = 10.48 WGEE75 pKa = 4.06 DD76 pKa = 3.51 VCDD79 pKa = 4.09 GDD81 pKa = 4.11 VPPGLVSAGLSIRR94 pKa = 11.84 SNTASCSLTSS104 pKa = 3.4
Molecular weight: 11.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.732
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.579
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.757
Rodwell 3.617
Grimsley 3.49
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.923
Patrickios 1.901
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.828
Protein with the highest isoelectric point:
>tr|Q19Y01|Q19Y01_9CAUD Uncharacterized protein OS=Mycobacterium phage Wildcat OX=373415 GN=59 PE=4 SV=1
MM1 pKa = 7.14 NPEE4 pKa = 3.59 RR5 pKa = 11.84 RR6 pKa = 11.84 ITEE9 pKa = 4.22 IIEE12 pKa = 3.63 AHH14 pKa = 6.15 QPDD17 pKa = 4.4 EE18 pKa = 4.66 HH19 pKa = 7.5 AGHH22 pKa = 7.65 LANEE26 pKa = 4.97 RR27 pKa = 11.84 WCKK30 pKa = 9.81 GCPRR34 pKa = 11.84 NSFRR38 pKa = 11.84 VWPWSEE44 pKa = 4.46 FPAHH48 pKa = 4.94 QAEE51 pKa = 4.64 MIMNEE56 pKa = 4.03 LGIHH60 pKa = 5.53 RR61 pKa = 11.84 VRR63 pKa = 11.84 HH64 pKa = 5.59 SFWSWGRR71 pKa = 11.84 RR72 pKa = 11.84 KK73 pKa = 7.74 TRR75 pKa = 11.84 HH76 pKa = 4.21 YY77 pKa = 9.8 WRR79 pKa = 11.84 LRR81 pKa = 3.39
Molecular weight: 10.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.334
IPC2_protein 9.224
IPC_protein 10.014
Toseland 10.365
ProMoST 10.716
Dawson 10.467
Bjellqvist 10.233
Wikipedia 10.701
Rodwell 10.511
Grimsley 10.526
Solomon 10.643
Lehninger 10.613
Nozaki 10.423
DTASelect 10.204
Thurlkill 10.379
EMBOSS 10.774
Sillero 10.423
Patrickios 10.394
IPC_peptide 10.643
IPC2_peptide 9.619
IPC2.peptide.svr19 8.677
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
148
0
148
23412
28
1983
158.2
17.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.91 ± 0.36
1.145 ± 0.135
6.591 ± 0.212
6.501 ± 0.263
3.485 ± 0.138
8.252 ± 0.431
2.435 ± 0.158
4.805 ± 0.155
4.31 ± 0.219
7.744 ± 0.187
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.695 ± 0.145
3.814 ± 0.175
5.326 ± 0.163
3.669 ± 0.264
6.245 ± 0.306
5.335 ± 0.156
5.899 ± 0.205
7.052 ± 0.228
2.559 ± 0.143
3.229 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here