Mycobacterium phage Wildcat

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wildcatvirus; Mycobacterium virus Wildcat

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 148 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q19Y45|Q19Y45_9CAUD Uncharacterized protein OS=Mycobacterium phage Wildcat OX=373415 GN=15 PE=4 SV=1
MM1 pKa = 7.63TDD3 pKa = 3.35WDD5 pKa = 4.11WVDD8 pKa = 3.15QHH10 pKa = 8.24IDD12 pKa = 3.33TQLTIQEE19 pKa = 4.31RR20 pKa = 11.84GCYY23 pKa = 9.72SNPFFEE29 pKa = 5.64LKK31 pKa = 10.41DD32 pKa = 3.82DD33 pKa = 4.44LVVTVDD39 pKa = 3.69GARR42 pKa = 11.84WTPTGWGEE50 pKa = 3.76ATIGGEE56 pKa = 3.92RR57 pKa = 11.84YY58 pKa = 9.14LHH60 pKa = 6.22NWDD63 pKa = 4.39EE64 pKa = 4.61SPADD68 pKa = 3.61VPDD71 pKa = 3.33YY72 pKa = 10.48WGEE75 pKa = 4.06DD76 pKa = 3.51VCDD79 pKa = 4.09GDD81 pKa = 4.11VPPGLVSAGLSIRR94 pKa = 11.84SNTASCSLTSS104 pKa = 3.4

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q19Y01|Q19Y01_9CAUD Uncharacterized protein OS=Mycobacterium phage Wildcat OX=373415 GN=59 PE=4 SV=1
MM1 pKa = 7.14NPEE4 pKa = 3.59RR5 pKa = 11.84RR6 pKa = 11.84ITEE9 pKa = 4.22IIEE12 pKa = 3.63AHH14 pKa = 6.15QPDD17 pKa = 4.4EE18 pKa = 4.66HH19 pKa = 7.5AGHH22 pKa = 7.65LANEE26 pKa = 4.97RR27 pKa = 11.84WCKK30 pKa = 9.81GCPRR34 pKa = 11.84NSFRR38 pKa = 11.84VWPWSEE44 pKa = 4.46FPAHH48 pKa = 4.94QAEE51 pKa = 4.64MIMNEE56 pKa = 4.03LGIHH60 pKa = 5.53RR61 pKa = 11.84VRR63 pKa = 11.84HH64 pKa = 5.59SFWSWGRR71 pKa = 11.84RR72 pKa = 11.84KK73 pKa = 7.74TRR75 pKa = 11.84HH76 pKa = 4.21YY77 pKa = 9.8WRR79 pKa = 11.84LRR81 pKa = 3.39

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

148

0

148

23412

28

1983

158.2

17.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.91 ± 0.36

1.145 ± 0.135

6.591 ± 0.212

6.501 ± 0.263

3.485 ± 0.138

8.252 ± 0.431

2.435 ± 0.158

4.805 ± 0.155

4.31 ± 0.219

7.744 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.145

3.814 ± 0.175

5.326 ± 0.163

3.669 ± 0.264

6.245 ± 0.306

5.335 ± 0.156

5.899 ± 0.205

7.052 ± 0.228

2.559 ± 0.143

3.229 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski