Amino acid dipepetide frequency for Mycobacterium phage Wildcat

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.269AlaAla: 9.269 ± 0.813
0.812AlaCys: 0.812 ± 0.2
5.382AlaAsp: 5.382 ± 0.463
6.621AlaGlu: 6.621 ± 0.534
3.033AlaPhe: 3.033 ± 0.33
6.963AlaGly: 6.963 ± 0.912
1.794AlaHis: 1.794 ± 0.276
5.04AlaIle: 5.04 ± 0.49
4.827AlaLys: 4.827 ± 0.503
6.749AlaLeu: 6.749 ± 0.689
3.033AlaMet: 3.033 ± 0.331
3.075AlaAsn: 3.075 ± 0.384
4.229AlaPro: 4.229 ± 0.416
3.802AlaGln: 3.802 ± 0.57
5.169AlaArg: 5.169 ± 0.579
4.827AlaSer: 4.827 ± 0.471
5.169AlaThr: 5.169 ± 0.631
5.083AlaVal: 5.083 ± 0.567
1.666AlaTrp: 1.666 ± 0.258
2.563AlaTyr: 2.563 ± 0.313
0.0AlaXaa: 0.0 ± 0.0
Cys
1.111CysAla: 1.111 ± 0.242
0.128CysCys: 0.128 ± 0.107
0.427CysAsp: 0.427 ± 0.163
0.854CysGlu: 0.854 ± 0.192
0.47CysPhe: 0.47 ± 0.13
1.324CysGly: 1.324 ± 0.29
0.513CysHis: 0.513 ± 0.168
0.641CysIle: 0.641 ± 0.188
0.812CysLys: 0.812 ± 0.206
0.641CysLeu: 0.641 ± 0.175
0.256CysMet: 0.256 ± 0.099
0.555CysAsn: 0.555 ± 0.15
0.641CysPro: 0.641 ± 0.184
0.256CysGln: 0.256 ± 0.114
0.854CysArg: 0.854 ± 0.222
0.555CysSer: 0.555 ± 0.215
0.256CysThr: 0.256 ± 0.106
0.47CysVal: 0.47 ± 0.125
0.384CysTrp: 0.384 ± 0.13
0.299CysTyr: 0.299 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
6.236AspAla: 6.236 ± 0.478
0.555AspCys: 0.555 ± 0.176
5.339AspAsp: 5.339 ± 0.57
5.04AspGlu: 5.04 ± 0.572
2.947AspPhe: 2.947 ± 0.423
4.741AspGly: 4.741 ± 0.441
1.367AspHis: 1.367 ± 0.252
2.947AspIle: 2.947 ± 0.402
2.477AspLys: 2.477 ± 0.315
5.724AspLeu: 5.724 ± 0.535
1.965AspMet: 1.965 ± 0.323
1.794AspAsn: 1.794 ± 0.279
4.571AspPro: 4.571 ± 0.453
2.136AspGln: 2.136 ± 0.298
3.673AspArg: 3.673 ± 0.371
2.606AspSer: 2.606 ± 0.367
3.289AspThr: 3.289 ± 0.392
4.314AspVal: 4.314 ± 0.501
1.879AspTrp: 1.879 ± 0.304
2.307AspTyr: 2.307 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
5.809GluAla: 5.809 ± 0.574
0.598GluCys: 0.598 ± 0.169
4.143GluAsp: 4.143 ± 0.537
4.314GluGlu: 4.314 ± 0.587
2.477GluPhe: 2.477 ± 0.34
4.741GluGly: 4.741 ± 0.482
1.495GluHis: 1.495 ± 0.252
3.161GluIle: 3.161 ± 0.357
2.435GluLys: 2.435 ± 0.338
6.877GluLeu: 6.877 ± 0.653
1.794GluMet: 1.794 ± 0.337
2.008GluAsn: 2.008 ± 0.318
2.435GluPro: 2.435 ± 0.367
2.008GluGln: 2.008 ± 0.265
3.844GluArg: 3.844 ± 0.476
3.716GluSer: 3.716 ± 0.42
3.332GluThr: 3.332 ± 0.363
4.741GluVal: 4.741 ± 0.458
2.477GluTrp: 2.477 ± 0.275
2.606GluTyr: 2.606 ± 0.421
0.0GluXaa: 0.0 ± 0.0
Phe
2.734PheAla: 2.734 ± 0.354
0.299PheCys: 0.299 ± 0.14
2.905PheAsp: 2.905 ± 0.352
2.648PheGlu: 2.648 ± 0.412
0.982PhePhe: 0.982 ± 0.242
3.545PheGly: 3.545 ± 0.587
0.769PheHis: 0.769 ± 0.15
1.58PheIle: 1.58 ± 0.226
1.153PheLys: 1.153 ± 0.204
2.221PheLeu: 2.221 ± 0.399
0.94PheMet: 0.94 ± 0.201
1.666PheAsn: 1.666 ± 0.204
1.196PhePro: 1.196 ± 0.25
0.726PheGln: 0.726 ± 0.188
2.477PheArg: 2.477 ± 0.315
2.264PheSer: 2.264 ± 0.331
2.05PheThr: 2.05 ± 0.337
2.905PheVal: 2.905 ± 0.345
0.683PheTrp: 0.683 ± 0.171
1.111PheTyr: 1.111 ± 0.291
0.0PheXaa: 0.0 ± 0.0
Gly
7.005GlyAla: 7.005 ± 0.86
0.812GlyCys: 0.812 ± 0.155
4.827GlyAsp: 4.827 ± 0.579
4.998GlyGlu: 4.998 ± 0.574
2.734GlyPhe: 2.734 ± 0.401
6.92GlyGly: 6.92 ± 1.059
2.093GlyHis: 2.093 ± 0.298
4.314GlyIle: 4.314 ± 0.423
4.186GlyLys: 4.186 ± 0.412
5.852GlyLeu: 5.852 ± 0.614
1.367GlyMet: 1.367 ± 0.251
4.058GlyAsn: 4.058 ± 0.569
3.588GlyPro: 3.588 ± 0.547
3.204GlyGln: 3.204 ± 0.424
4.186GlyArg: 4.186 ± 0.508
4.998GlySer: 4.998 ± 0.743
6.023GlyThr: 6.023 ± 0.661
5.767GlyVal: 5.767 ± 0.662
2.349GlyTrp: 2.349 ± 0.361
3.246GlyTyr: 3.246 ± 0.371
0.0GlyXaa: 0.0 ± 0.0
His
1.709HisAla: 1.709 ± 0.319
0.513HisCys: 0.513 ± 0.145
1.41HisAsp: 1.41 ± 0.273
1.111HisGlu: 1.111 ± 0.24
1.196HisPhe: 1.196 ± 0.234
2.307HisGly: 2.307 ± 0.344
0.812HisHis: 0.812 ± 0.172
1.281HisIle: 1.281 ± 0.281
1.495HisLys: 1.495 ± 0.254
2.05HisLeu: 2.05 ± 0.299
0.555HisMet: 0.555 ± 0.169
0.812HisAsn: 0.812 ± 0.203
2.008HisPro: 2.008 ± 0.283
0.555HisGln: 0.555 ± 0.161
1.41HisArg: 1.41 ± 0.285
0.982HisSer: 0.982 ± 0.225
1.58HisThr: 1.58 ± 0.217
1.239HisVal: 1.239 ± 0.221
0.513HisTrp: 0.513 ± 0.157
0.812HisTyr: 0.812 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
4.229IleAla: 4.229 ± 0.514
0.513IleCys: 0.513 ± 0.153
3.631IleAsp: 3.631 ± 0.362
4.528IleGlu: 4.528 ± 0.603
1.41IlePhe: 1.41 ± 0.281
3.033IleGly: 3.033 ± 0.495
1.324IleHis: 1.324 ± 0.204
2.05IleIle: 2.05 ± 0.286
1.965IleLys: 1.965 ± 0.313
3.545IleLeu: 3.545 ± 0.431
1.111IleMet: 1.111 ± 0.218
2.093IleAsn: 2.093 ± 0.365
3.802IlePro: 3.802 ± 0.325
1.41IleGln: 1.41 ± 0.263
2.691IleArg: 2.691 ± 0.335
2.392IleSer: 2.392 ± 0.312
3.118IleThr: 3.118 ± 0.413
3.844IleVal: 3.844 ± 0.35
0.641IleTrp: 0.641 ± 0.145
0.726IleTyr: 0.726 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
4.528LysAla: 4.528 ± 0.517
0.513LysCys: 0.513 ± 0.167
2.178LysAsp: 2.178 ± 0.322
1.965LysGlu: 1.965 ± 0.371
1.196LysPhe: 1.196 ± 0.211
3.802LysGly: 3.802 ± 0.399
0.726LysHis: 0.726 ± 0.176
2.136LysIle: 2.136 ± 0.363
1.709LysLys: 1.709 ± 0.308
3.588LysLeu: 3.588 ± 0.498
1.324LysMet: 1.324 ± 0.27
1.837LysAsn: 1.837 ± 0.247
2.008LysPro: 2.008 ± 0.276
1.709LysGln: 1.709 ± 0.281
3.204LysArg: 3.204 ± 0.535
2.221LysSer: 2.221 ± 0.339
2.734LysThr: 2.734 ± 0.401
3.161LysVal: 3.161 ± 0.33
1.068LysTrp: 1.068 ± 0.176
1.495LysTyr: 1.495 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
7.219LeuAla: 7.219 ± 0.632
0.982LeuCys: 0.982 ± 0.244
5.468LeuAsp: 5.468 ± 0.618
4.613LeuGlu: 4.613 ± 0.456
2.349LeuPhe: 2.349 ± 0.336
6.792LeuGly: 6.792 ± 0.844
1.879LeuHis: 1.879 ± 0.358
2.99LeuIle: 2.99 ± 0.37
3.46LeuLys: 3.46 ± 0.393
5.468LeuLeu: 5.468 ± 0.489
2.221LeuMet: 2.221 ± 0.323
3.588LeuAsn: 3.588 ± 0.408
4.955LeuPro: 4.955 ± 0.548
2.264LeuGln: 2.264 ± 0.307
5.382LeuArg: 5.382 ± 0.497
4.058LeuSer: 4.058 ± 0.425
4.528LeuThr: 4.528 ± 0.522
5.083LeuVal: 5.083 ± 0.576
1.324LeuTrp: 1.324 ± 0.293
2.819LeuTyr: 2.819 ± 0.414
0.0LeuXaa: 0.0 ± 0.0
Met
2.691MetAla: 2.691 ± 0.363
0.171MetCys: 0.171 ± 0.081
1.794MetAsp: 1.794 ± 0.278
1.666MetGlu: 1.666 ± 0.333
0.769MetPhe: 0.769 ± 0.178
2.349MetGly: 2.349 ± 0.373
0.299MetHis: 0.299 ± 0.107
1.367MetIle: 1.367 ± 0.23
1.196MetLys: 1.196 ± 0.271
2.178MetLeu: 2.178 ± 0.319
0.555MetMet: 0.555 ± 0.159
1.239MetAsn: 1.239 ± 0.205
1.452MetPro: 1.452 ± 0.226
1.068MetGln: 1.068 ± 0.24
2.05MetArg: 2.05 ± 0.312
2.05MetSer: 2.05 ± 0.3
1.239MetThr: 1.239 ± 0.228
1.367MetVal: 1.367 ± 0.257
0.641MetTrp: 0.641 ± 0.14
0.812MetTyr: 0.812 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
3.075AsnAla: 3.075 ± 0.468
0.256AsnCys: 0.256 ± 0.123
2.264AsnAsp: 2.264 ± 0.362
1.922AsnGlu: 1.922 ± 0.264
1.196AsnPhe: 1.196 ± 0.258
3.289AsnGly: 3.289 ± 0.35
0.982AsnHis: 0.982 ± 0.227
1.709AsnIle: 1.709 ± 0.347
1.58AsnLys: 1.58 ± 0.278
3.503AsnLeu: 3.503 ± 0.474
0.769AsnMet: 0.769 ± 0.164
1.538AsnAsn: 1.538 ± 0.256
3.332AsnPro: 3.332 ± 0.371
1.623AsnGln: 1.623 ± 0.291
2.05AsnArg: 2.05 ± 0.319
2.05AsnSer: 2.05 ± 0.281
2.264AsnThr: 2.264 ± 0.305
2.648AsnVal: 2.648 ± 0.35
1.025AsnTrp: 1.025 ± 0.2
1.068AsnTyr: 1.068 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
4.058ProAla: 4.058 ± 0.467
0.47ProCys: 0.47 ± 0.143
4.101ProAsp: 4.101 ± 0.376
4.656ProGlu: 4.656 ± 0.424
1.837ProPhe: 1.837 ± 0.247
4.784ProGly: 4.784 ± 0.683
1.922ProHis: 1.922 ± 0.295
2.05ProIle: 2.05 ± 0.27
2.52ProLys: 2.52 ± 0.406
3.033ProLeu: 3.033 ± 0.425
1.367ProMet: 1.367 ± 0.303
2.05ProAsn: 2.05 ± 0.36
3.033ProPro: 3.033 ± 0.379
2.264ProGln: 2.264 ± 0.339
2.691ProArg: 2.691 ± 0.354
2.691ProSer: 2.691 ± 0.324
3.204ProThr: 3.204 ± 0.406
3.844ProVal: 3.844 ± 0.472
1.324ProTrp: 1.324 ± 0.229
1.367ProTyr: 1.367 ± 0.243
0.0ProXaa: 0.0 ± 0.0
Gln
4.143GlnAla: 4.143 ± 0.684
0.513GlnCys: 0.513 ± 0.167
1.879GlnAsp: 1.879 ± 0.278
1.879GlnGlu: 1.879 ± 0.236
1.538GlnPhe: 1.538 ± 0.311
2.563GlnGly: 2.563 ± 0.488
0.982GlnHis: 0.982 ± 0.174
1.879GlnIle: 1.879 ± 0.24
1.153GlnLys: 1.153 ± 0.211
3.033GlnLeu: 3.033 ± 0.403
1.324GlnMet: 1.324 ± 0.249
1.495GlnAsn: 1.495 ± 0.207
1.837GlnPro: 1.837 ± 0.291
1.751GlnGln: 1.751 ± 0.389
2.648GlnArg: 2.648 ± 0.355
2.008GlnSer: 2.008 ± 0.271
1.623GlnThr: 1.623 ± 0.244
2.221GlnVal: 2.221 ± 0.334
1.153GlnTrp: 1.153 ± 0.219
1.068GlnTyr: 1.068 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
4.827ArgAla: 4.827 ± 0.462
1.495ArgCys: 1.495 ± 0.367
4.015ArgAsp: 4.015 ± 0.411
4.656ArgGlu: 4.656 ± 0.493
2.349ArgPhe: 2.349 ± 0.353
4.741ArgGly: 4.741 ± 0.508
1.452ArgHis: 1.452 ± 0.23
3.075ArgIle: 3.075 ± 0.451
3.033ArgLys: 3.033 ± 0.455
3.93ArgLeu: 3.93 ± 0.495
2.136ArgMet: 2.136 ± 0.374
2.477ArgAsn: 2.477 ± 0.333
1.879ArgPro: 1.879 ± 0.247
2.477ArgGln: 2.477 ± 0.308
4.271ArgArg: 4.271 ± 0.625
2.99ArgSer: 2.99 ± 0.378
3.417ArgThr: 3.417 ± 0.357
5.382ArgVal: 5.382 ± 0.493
1.879ArgTrp: 1.879 ± 0.294
1.965ArgTyr: 1.965 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
4.955SerAla: 4.955 ± 0.506
0.598SerCys: 0.598 ± 0.177
3.374SerAsp: 3.374 ± 0.4
2.862SerGlu: 2.862 ± 0.311
1.794SerPhe: 1.794 ± 0.26
4.912SerGly: 4.912 ± 0.625
1.367SerHis: 1.367 ± 0.269
3.033SerIle: 3.033 ± 0.373
2.136SerLys: 2.136 ± 0.32
4.314SerLeu: 4.314 ± 0.446
1.367SerMet: 1.367 ± 0.214
1.922SerAsn: 1.922 ± 0.403
2.606SerPro: 2.606 ± 0.36
2.776SerGln: 2.776 ± 0.319
3.417SerArg: 3.417 ± 0.458
2.819SerSer: 2.819 ± 0.436
2.606SerThr: 2.606 ± 0.312
3.545SerVal: 3.545 ± 0.471
1.281SerTrp: 1.281 ± 0.274
1.623SerTyr: 1.623 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
4.4ThrAla: 4.4 ± 0.506
0.812ThrCys: 0.812 ± 0.209
3.545ThrAsp: 3.545 ± 0.446
3.204ThrGlu: 3.204 ± 0.419
2.477ThrPhe: 2.477 ± 0.363
5.596ThrGly: 5.596 ± 0.66
1.153ThrHis: 1.153 ± 0.225
3.673ThrIle: 3.673 ± 0.5
2.093ThrLys: 2.093 ± 0.342
4.699ThrLeu: 4.699 ± 0.44
1.495ThrMet: 1.495 ± 0.257
1.495ThrAsn: 1.495 ± 0.266
3.46ThrPro: 3.46 ± 0.603
1.965ThrGln: 1.965 ± 0.28
3.161ThrArg: 3.161 ± 0.364
3.545ThrSer: 3.545 ± 0.471
3.93ThrThr: 3.93 ± 0.48
4.528ThrVal: 4.528 ± 0.478
1.709ThrTrp: 1.709 ± 0.316
2.05ThrTyr: 2.05 ± 0.273
0.0ThrXaa: 0.0 ± 0.0
Val
6.236ValAla: 6.236 ± 0.6
0.769ValCys: 0.769 ± 0.186
4.784ValAsp: 4.784 ± 0.374
4.101ValGlu: 4.101 ± 0.354
2.349ValPhe: 2.349 ± 0.335
5.638ValGly: 5.638 ± 0.682
1.452ValHis: 1.452 ± 0.227
2.648ValIle: 2.648 ± 0.326
2.563ValLys: 2.563 ± 0.318
5.211ValLeu: 5.211 ± 0.492
1.666ValMet: 1.666 ± 0.271
3.033ValAsn: 3.033 ± 0.354
3.887ValPro: 3.887 ± 0.484
2.563ValGln: 2.563 ± 0.333
4.485ValArg: 4.485 ± 0.421
4.314ValSer: 4.314 ± 0.432
5.254ValThr: 5.254 ± 0.583
5.596ValVal: 5.596 ± 0.692
1.709ValTrp: 1.709 ± 0.309
2.264ValTyr: 2.264 ± 0.358
0.0ValXaa: 0.0 ± 0.0
Trp
1.922TrpAla: 1.922 ± 0.248
0.427TrpCys: 0.427 ± 0.157
2.093TrpAsp: 2.093 ± 0.306
1.623TrpGlu: 1.623 ± 0.212
0.555TrpPhe: 0.555 ± 0.167
1.879TrpGly: 1.879 ± 0.32
0.683TrpHis: 0.683 ± 0.179
1.324TrpIle: 1.324 ± 0.253
1.111TrpLys: 1.111 ± 0.211
2.349TrpLeu: 2.349 ± 0.339
0.683TrpMet: 0.683 ± 0.165
0.555TrpAsn: 0.555 ± 0.148
0.769TrpPro: 0.769 ± 0.175
1.111TrpGln: 1.111 ± 0.221
1.837TrpArg: 1.837 ± 0.331
1.025TrpSer: 1.025 ± 0.217
1.58TrpThr: 1.58 ± 0.224
2.392TrpVal: 2.392 ± 0.38
0.726TrpTrp: 0.726 ± 0.207
0.94TrpTyr: 0.94 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.947TyrAla: 2.947 ± 0.354
0.214TyrCys: 0.214 ± 0.112
2.349TyrAsp: 2.349 ± 0.375
1.751TyrGlu: 1.751 ± 0.314
1.196TyrPhe: 1.196 ± 0.22
2.606TyrGly: 2.606 ± 0.338
1.281TyrHis: 1.281 ± 0.272
1.153TyrIle: 1.153 ± 0.25
1.196TyrLys: 1.196 ± 0.228
2.477TyrLeu: 2.477 ± 0.405
0.982TyrMet: 0.982 ± 0.185
0.854TyrAsn: 0.854 ± 0.214
1.58TyrPro: 1.58 ± 0.297
1.025TyrGln: 1.025 ± 0.213
2.947TyrArg: 2.947 ± 0.424
1.239TyrSer: 1.239 ± 0.251
1.794TyrThr: 1.794 ± 0.318
2.392TyrVal: 2.392 ± 0.344
1.153TyrTrp: 1.153 ± 0.225
1.153TyrTyr: 1.153 ± 0.205
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 148 proteins (23412 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski