Paxillus rubicundulus Ve08.2h10
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 21906 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D0D2A3|A0A0D0D2A3_9AGAM Unplaced genomic scaffold scaffold_2061 whole genome shotgun sequence OS=Paxillus rubicundulus Ve08.2h10 OX=930991 GN=PAXRUDRAFT_834924 PE=4 SV=1
MM1 pKa = 7.01 STIINVKK8 pKa = 7.58 TVNWLNGFSLKK19 pKa = 10.6 PLDD22 pKa = 4.63 LSIHH26 pKa = 5.75 FLGHH30 pKa = 6.27 VSLQDD35 pKa = 3.34 IPEE38 pKa = 4.92 HH39 pKa = 7.57 IPIQVSICLGDD50 pKa = 3.32 EE51 pKa = 4.6 WIDD54 pKa = 3.35 QLFPIVEE61 pKa = 4.25 ALLADD66 pKa = 3.94 NMSEE70 pKa = 4.24 SADD73 pKa = 3.66 DD74 pKa = 3.76 VSPEE78 pKa = 4.13 LFTFLPLPSQFDD90 pKa = 4.0 PYY92 pKa = 11.39 DD93 pKa = 3.73 GLEE96 pKa = 4.03 WQSLAKK102 pKa = 10.29 EE103 pKa = 4.06 IEE105 pKa = 3.98 HH106 pKa = 7.02 WLLQDD111 pKa = 3.37 PAFNMMHH118 pKa = 6.83 PGCYY122 pKa = 9.23 ILMWEE127 pKa = 4.71 LFWMAFVTVFPSFPYY142 pKa = 10.2 GQWPDD147 pKa = 3.25 WDD149 pKa = 3.27 PWIPVDD155 pKa = 5.7 GYY157 pKa = 11.27 FILDD161 pKa = 3.75 WVADD165 pKa = 4.01 LDD167 pKa = 4.27 EE168 pKa = 5.37 PEE170 pKa = 4.73 PAPVPAIVCEE180 pKa = 4.52 SVWEE184 pKa = 3.99 QSKK187 pKa = 10.66 AAVDD191 pKa = 3.52
Molecular weight: 21.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.834
Dawson 3.706
Bjellqvist 3.897
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.414
Solomon 3.694
Lehninger 3.643
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.554
EMBOSS 3.643
Sillero 3.834
Patrickios 0.693
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A0D0DNQ2|A0A0D0DNQ2_9AGAM Unplaced genomic scaffold scaffold_1228 whole genome shotgun sequence (Fragment) OS=Paxillus rubicundulus Ve08.2h10 OX=930991 GN=PAXRUDRAFT_96613 PE=4 SV=1
MM1 pKa = 7.59 GLLLHH6 pKa = 6.99 LIRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 PFPSPVRR19 pKa = 11.84 ATLAFTSHH27 pKa = 7.8 PIHH30 pKa = 6.88 PLHH33 pKa = 6.75 LPVHH37 pKa = 6.25 SGIPNVSTTPPLVSPQASGFWW58 pKa = 3.32
Molecular weight: 6.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.386
IPC2_protein 10.818
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.106
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.901
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.16
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21895
11
21906
5778669
49
5106
263.8
29.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.986 ± 0.017
1.691 ± 0.011
5.333 ± 0.014
5.706 ± 0.02
3.654 ± 0.012
6.268 ± 0.018
3.06 ± 0.012
4.918 ± 0.013
4.675 ± 0.017
9.338 ± 0.021
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.317 ± 0.008
3.5 ± 0.011
6.525 ± 0.027
4.055 ± 0.014
5.768 ± 0.019
8.708 ± 0.023
6.084 ± 0.01
6.3 ± 0.013
1.565 ± 0.008
2.55 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here