Proteus phage phiP4-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I6PFF3|A0A2I6PFF3_9CAUD RNA ligase 2 OS=Proteus phage phiP4-3 OX=2065203 GN=phiP43_097 PE=3 SV=1
MM1 pKa = 7.55KK2 pKa = 10.09KK3 pKa = 10.61YY4 pKa = 10.4IVWCEE9 pKa = 3.68DD10 pKa = 3.22LEE12 pKa = 5.42LGFTTSNYY20 pKa = 10.02QLALDD25 pKa = 3.86IKK27 pKa = 10.77NNNTEE32 pKa = 4.16CIIDD36 pKa = 3.54EE37 pKa = 4.33SEE39 pKa = 4.5YY40 pKa = 10.74PGIAAMAFDD49 pKa = 4.56FCDD52 pKa = 3.42LTEE55 pKa = 4.37DD56 pKa = 3.82TEE58 pKa = 4.28LTIIEE63 pKa = 4.39VKK65 pKa = 10.65

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I6PFD0|A0A2I6PFD0_9CAUD Baseplate hub assembly protein OS=Proteus phage phiP4-3 OX=2065203 GN=phiP43_080 PE=4 SV=1
MM1 pKa = 7.58SEE3 pKa = 3.69RR4 pKa = 11.84KK5 pKa = 9.54GYY7 pKa = 9.36KK8 pKa = 9.49RR9 pKa = 11.84KK10 pKa = 7.77TNKK13 pKa = 10.2RR14 pKa = 11.84INQEE18 pKa = 3.86LNKK21 pKa = 10.55LVGFVKK27 pKa = 10.29RR28 pKa = 11.84QEE30 pKa = 3.69NGRR33 pKa = 11.84AKK35 pKa = 10.51FLANLGNKK43 pKa = 8.71KK44 pKa = 10.29

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

270

0

270

53047

41

1218

196.5

22.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.909 ± 0.146

1.25 ± 0.076

6.5 ± 0.108

7.397 ± 0.126

4.526 ± 0.101

5.644 ± 0.156

1.738 ± 0.067

7.927 ± 0.151

8.77 ± 0.175

8.381 ± 0.124

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.076

6.032 ± 0.114

3.386 ± 0.084

3.182 ± 0.087

3.61 ± 0.1

6.845 ± 0.107

5.606 ± 0.145

6.002 ± 0.108

1.252 ± 0.055

4.675 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski