Amino acid dipepetide frequency for Proteus phage phiP4-3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.507AlaAla: 2.507 ± 0.243
0.452AlaCys: 0.452 ± 0.088
2.507AlaAsp: 2.507 ± 0.22
3.601AlaGlu: 3.601 ± 0.28
2.111AlaPhe: 2.111 ± 0.176
2.884AlaGly: 2.884 ± 0.287
0.811AlaHis: 0.811 ± 0.129
4.11AlaIle: 4.11 ± 0.259
4.298AlaLys: 4.298 ± 0.291
5.241AlaLeu: 5.241 ± 0.322
1.338AlaMet: 1.338 ± 0.171
2.319AlaAsn: 2.319 ± 0.223
1.395AlaPro: 1.395 ± 0.172
1.697AlaGln: 1.697 ± 0.183
1.772AlaArg: 1.772 ± 0.202
3.865AlaSer: 3.865 ± 0.277
2.375AlaThr: 2.375 ± 0.242
2.941AlaVal: 2.941 ± 0.293
0.754AlaTrp: 0.754 ± 0.124
2.111AlaTyr: 2.111 ± 0.206
0.0AlaXaa: 0.0 ± 0.0
Cys
0.547CysAla: 0.547 ± 0.09
0.283CysCys: 0.283 ± 0.073
0.735CysAsp: 0.735 ± 0.103
1.188CysGlu: 1.188 ± 0.158
0.584CysPhe: 0.584 ± 0.112
0.698CysGly: 0.698 ± 0.133
0.283CysHis: 0.283 ± 0.075
0.98CysIle: 0.98 ± 0.149
0.98CysLys: 0.98 ± 0.173
0.754CysLeu: 0.754 ± 0.11
0.377CysMet: 0.377 ± 0.082
0.735CysAsn: 0.735 ± 0.107
0.566CysPro: 0.566 ± 0.1
0.302CysGln: 0.302 ± 0.068
0.584CysArg: 0.584 ± 0.094
1.075CysSer: 1.075 ± 0.153
0.528CysThr: 0.528 ± 0.088
0.735CysVal: 0.735 ± 0.111
0.038CysTrp: 0.038 ± 0.027
0.528CysTyr: 0.528 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
2.771AspAla: 2.771 ± 0.238
0.773AspCys: 0.773 ± 0.114
4.072AspAsp: 4.072 ± 0.256
3.751AspGlu: 3.751 ± 0.278
3.808AspPhe: 3.808 ± 0.285
3.676AspGly: 3.676 ± 0.229
0.924AspHis: 0.924 ± 0.136
5.844AspIle: 5.844 ± 0.306
5.655AspLys: 5.655 ± 0.318
5.693AspLeu: 5.693 ± 0.346
1.546AspMet: 1.546 ± 0.19
3.827AspAsn: 3.827 ± 0.242
2.262AspPro: 2.262 ± 0.193
1.715AspGln: 1.715 ± 0.185
1.942AspArg: 1.942 ± 0.209
4.581AspSer: 4.581 ± 0.33
3.393AspThr: 3.393 ± 0.375
3.978AspVal: 3.978 ± 0.267
1.301AspTrp: 1.301 ± 0.164
3.488AspTyr: 3.488 ± 0.283
0.0AspXaa: 0.0 ± 0.0
Glu
4.11GluAla: 4.11 ± 0.314
0.905GluCys: 0.905 ± 0.121
4.807GluAsp: 4.807 ± 0.311
5.769GluGlu: 5.769 ± 0.358
3.525GluPhe: 3.525 ± 0.238
3.638GluGly: 3.638 ± 0.27
1.414GluHis: 1.414 ± 0.164
5.505GluIle: 5.505 ± 0.325
5.41GluLys: 5.41 ± 0.367
7.955GluLeu: 7.955 ± 0.365
1.847GluMet: 1.847 ± 0.179
4.034GluAsn: 4.034 ± 0.25
1.565GluPro: 1.565 ± 0.178
2.752GluGln: 2.752 ± 0.258
2.545GluArg: 2.545 ± 0.212
4.92GluSer: 4.92 ± 0.381
4.242GluThr: 4.242 ± 0.275
4.468GluVal: 4.468 ± 0.307
0.811GluTrp: 0.811 ± 0.139
3.751GluTyr: 3.751 ± 0.267
0.0GluXaa: 0.0 ± 0.0
Phe
1.829PheAla: 1.829 ± 0.198
0.471PheCys: 0.471 ± 0.095
3.808PheAsp: 3.808 ± 0.293
3.506PheGlu: 3.506 ± 0.258
1.602PhePhe: 1.602 ± 0.184
2.564PheGly: 2.564 ± 0.228
0.603PheHis: 0.603 ± 0.113
3.544PheIle: 3.544 ± 0.276
5.071PheLys: 5.071 ± 0.306
3.035PheLeu: 3.035 ± 0.234
1.357PheMet: 1.357 ± 0.187
3.111PheAsn: 3.111 ± 0.232
1.282PhePro: 1.282 ± 0.188
0.867PheGln: 0.867 ± 0.128
1.47PheArg: 1.47 ± 0.161
3.28PheSer: 3.28 ± 0.235
2.639PheThr: 2.639 ± 0.191
2.62PheVal: 2.62 ± 0.218
0.716PheTrp: 0.716 ± 0.137
1.885PheTyr: 1.885 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
2.733GlyAla: 2.733 ± 0.286
0.679GlyCys: 0.679 ± 0.122
3.469GlyAsp: 3.469 ± 0.288
3.582GlyGlu: 3.582 ± 0.292
2.865GlyPhe: 2.865 ± 0.24
2.658GlyGly: 2.658 ± 0.27
1.018GlyHis: 1.018 ± 0.149
4.694GlyIle: 4.694 ± 0.311
4.637GlyLys: 4.637 ± 0.339
5.335GlyLeu: 5.335 ± 0.315
1.282GlyMet: 1.282 ± 0.183
2.79GlyAsn: 2.79 ± 0.264
1.904GlyPro: 1.904 ± 0.195
2.111GlyGln: 2.111 ± 0.214
2.093GlyArg: 2.093 ± 0.184
3.544GlySer: 3.544 ± 0.278
3.865GlyThr: 3.865 ± 0.324
3.242GlyVal: 3.242 ± 0.195
0.735GlyTrp: 0.735 ± 0.128
3.205GlyTyr: 3.205 ± 0.263
0.0GlyXaa: 0.0 ± 0.0
His
0.773HisAla: 0.773 ± 0.139
0.302HisCys: 0.302 ± 0.086
1.131HisAsp: 1.131 ± 0.179
0.867HisGlu: 0.867 ± 0.125
0.867HisPhe: 0.867 ± 0.118
0.943HisGly: 0.943 ± 0.155
0.396HisHis: 0.396 ± 0.092
1.565HisIle: 1.565 ± 0.142
1.527HisLys: 1.527 ± 0.177
1.602HisLeu: 1.602 ± 0.21
0.434HisMet: 0.434 ± 0.094
1.018HisAsn: 1.018 ± 0.143
0.905HisPro: 0.905 ± 0.134
0.471HisGln: 0.471 ± 0.087
0.622HisArg: 0.622 ± 0.12
1.207HisSer: 1.207 ± 0.144
0.754HisThr: 0.754 ± 0.093
1.093HisVal: 1.093 ± 0.149
0.132HisTrp: 0.132 ± 0.046
0.773HisTyr: 0.773 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
3.638IleAla: 3.638 ± 0.275
1.112IleCys: 1.112 ± 0.142
5.542IleAsp: 5.542 ± 0.341
6.56IleGlu: 6.56 ± 0.373
2.96IlePhe: 2.96 ± 0.243
4.562IleGly: 4.562 ± 0.294
1.357IleHis: 1.357 ± 0.149
5.957IleIle: 5.957 ± 0.317
7.786IleLys: 7.786 ± 0.377
5.731IleLeu: 5.731 ± 0.32
1.885IleMet: 1.885 ± 0.187
5.542IleAsn: 5.542 ± 0.288
3.054IlePro: 3.054 ± 0.259
2.243IleGln: 2.243 ± 0.189
2.979IleArg: 2.979 ± 0.254
5.58IleSer: 5.58 ± 0.307
4.487IleThr: 4.487 ± 0.313
4.242IleVal: 4.242 ± 0.284
0.66IleTrp: 0.66 ± 0.129
3.393IleTyr: 3.393 ± 0.255
0.0IleXaa: 0.0 ± 0.0
Lys
4.788LysAla: 4.788 ± 0.278
1.15LysCys: 1.15 ± 0.182
6.108LysAsp: 6.108 ± 0.367
7.446LysGlu: 7.446 ± 0.408
3.77LysPhe: 3.77 ± 0.273
4.751LysGly: 4.751 ± 0.295
1.885LysHis: 1.885 ± 0.194
6.73LysIle: 6.73 ± 0.335
5.467LysLys: 5.467 ± 0.346
7.616LysLeu: 7.616 ± 0.407
2.338LysMet: 2.338 ± 0.23
5.146LysAsn: 5.146 ± 0.353
2.828LysPro: 2.828 ± 0.208
2.809LysGln: 2.809 ± 0.236
3.073LysArg: 3.073 ± 0.267
5.429LysSer: 5.429 ± 0.321
6.146LysThr: 6.146 ± 0.288
4.562LysVal: 4.562 ± 0.269
1.207LysTrp: 1.207 ± 0.131
4.449LysTyr: 4.449 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
4.694LeuAla: 4.694 ± 0.354
1.131LeuCys: 1.131 ± 0.152
5.58LeuAsp: 5.58 ± 0.336
6.9LeuGlu: 6.9 ± 0.398
3.45LeuPhe: 3.45 ± 0.271
4.92LeuGly: 4.92 ± 0.312
1.244LeuHis: 1.244 ± 0.18
5.599LeuIle: 5.599 ± 0.321
8.182LeuLys: 8.182 ± 0.381
5.75LeuLeu: 5.75 ± 0.308
2.13LeuMet: 2.13 ± 0.22
5.58LeuAsn: 5.58 ± 0.316
3.28LeuPro: 3.28 ± 0.267
2.451LeuGln: 2.451 ± 0.204
2.828LeuArg: 2.828 ± 0.226
6.296LeuSer: 6.296 ± 0.348
4.279LeuThr: 4.279 ± 0.289
4.788LeuVal: 4.788 ± 0.282
0.905LeuTrp: 0.905 ± 0.142
3.827LeuTyr: 3.827 ± 0.264
0.0LeuXaa: 0.0 ± 0.0
Met
1.338MetAla: 1.338 ± 0.171
0.32MetCys: 0.32 ± 0.08
1.489MetAsp: 1.489 ± 0.157
1.207MetGlu: 1.207 ± 0.142
1.15MetPhe: 1.15 ± 0.132
1.018MetGly: 1.018 ± 0.168
0.377MetHis: 0.377 ± 0.076
1.961MetIle: 1.961 ± 0.169
2.187MetLys: 2.187 ± 0.177
1.584MetLeu: 1.584 ± 0.187
0.396MetMet: 0.396 ± 0.085
1.753MetAsn: 1.753 ± 0.195
0.792MetPro: 0.792 ± 0.102
0.924MetGln: 0.924 ± 0.151
0.452MetArg: 0.452 ± 0.096
1.885MetSer: 1.885 ± 0.191
1.923MetThr: 1.923 ± 0.169
1.414MetVal: 1.414 ± 0.148
0.245MetTrp: 0.245 ± 0.066
1.263MetTyr: 1.263 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
2.941AsnAla: 2.941 ± 0.273
0.528AsnCys: 0.528 ± 0.094
3.657AsnAsp: 3.657 ± 0.245
3.488AsnGlu: 3.488 ± 0.26
2.47AsnPhe: 2.47 ± 0.229
4.015AsnGly: 4.015 ± 0.335
1.093AsnHis: 1.093 ± 0.124
4.694AsnIle: 4.694 ± 0.272
5.297AsnLys: 5.297 ± 0.3
5.109AsnLeu: 5.109 ± 0.287
1.47AsnMet: 1.47 ± 0.175
4.355AsnAsn: 4.355 ± 0.356
2.96AsnPro: 2.96 ± 0.206
1.942AsnGln: 1.942 ± 0.204
2.47AsnArg: 2.47 ± 0.233
3.789AsnSer: 3.789 ± 0.284
3.242AsnThr: 3.242 ± 0.259
3.657AsnVal: 3.657 ± 0.296
0.641AsnTrp: 0.641 ± 0.1
2.507AsnTyr: 2.507 ± 0.209
0.0AsnXaa: 0.0 ± 0.0
Pro
1.244ProAla: 1.244 ± 0.166
0.49ProCys: 0.49 ± 0.1
2.206ProAsp: 2.206 ± 0.225
3.62ProGlu: 3.62 ± 0.251
1.659ProPhe: 1.659 ± 0.197
2.111ProGly: 2.111 ± 0.246
0.698ProHis: 0.698 ± 0.113
2.602ProIle: 2.602 ± 0.231
3.111ProLys: 3.111 ± 0.291
2.338ProLeu: 2.338 ± 0.217
0.622ProMet: 0.622 ± 0.097
1.866ProAsn: 1.866 ± 0.21
0.773ProPro: 0.773 ± 0.145
1.018ProGln: 1.018 ± 0.126
1.282ProArg: 1.282 ± 0.15
2.187ProSer: 2.187 ± 0.182
1.847ProThr: 1.847 ± 0.171
2.319ProVal: 2.319 ± 0.243
0.509ProTrp: 0.509 ± 0.093
1.357ProTyr: 1.357 ± 0.141
0.0ProXaa: 0.0 ± 0.0
Gln
1.734GlnAla: 1.734 ± 0.184
0.302GlnCys: 0.302 ± 0.074
1.715GlnAsp: 1.715 ± 0.173
2.884GlnGlu: 2.884 ± 0.27
1.565GlnPhe: 1.565 ± 0.159
1.979GlnGly: 1.979 ± 0.184
0.49GlnHis: 0.49 ± 0.096
2.545GlnIle: 2.545 ± 0.228
2.338GlnLys: 2.338 ± 0.227
2.847GlnLeu: 2.847 ± 0.258
0.716GlnMet: 0.716 ± 0.116
1.659GlnAsn: 1.659 ± 0.158
0.773GlnPro: 0.773 ± 0.114
1.207GlnGln: 1.207 ± 0.165
1.32GlnArg: 1.32 ± 0.142
1.791GlnSer: 1.791 ± 0.176
1.546GlnThr: 1.546 ± 0.201
2.3GlnVal: 2.3 ± 0.202
0.509GlnTrp: 0.509 ± 0.1
1.602GlnTyr: 1.602 ± 0.164
0.0GlnXaa: 0.0 ± 0.0
Arg
1.998ArgAla: 1.998 ± 0.201
0.415ArgCys: 0.415 ± 0.079
2.017ArgAsp: 2.017 ± 0.191
2.602ArgGlu: 2.602 ± 0.24
1.772ArgPhe: 1.772 ± 0.216
1.961ArgGly: 1.961 ± 0.176
0.811ArgHis: 0.811 ± 0.134
2.979ArgIle: 2.979 ± 0.228
2.979ArgLys: 2.979 ± 0.232
3.318ArgLeu: 3.318 ± 0.261
0.754ArgMet: 0.754 ± 0.141
2.017ArgAsn: 2.017 ± 0.176
0.98ArgPro: 0.98 ± 0.121
1.188ArgGln: 1.188 ± 0.136
1.621ArgArg: 1.621 ± 0.227
2.017ArgSer: 2.017 ± 0.19
1.904ArgThr: 1.904 ± 0.188
2.677ArgVal: 2.677 ± 0.256
0.452ArgTrp: 0.452 ± 0.096
1.621ArgTyr: 1.621 ± 0.181
0.0ArgXaa: 0.0 ± 0.0
Ser
3.035SerAla: 3.035 ± 0.255
1.093SerCys: 1.093 ± 0.142
4.543SerAsp: 4.543 ± 0.369
4.543SerGlu: 4.543 ± 0.327
2.828SerPhe: 2.828 ± 0.212
4.392SerGly: 4.392 ± 0.335
1.112SerHis: 1.112 ± 0.128
6.202SerIle: 6.202 ± 0.327
6.843SerLys: 6.843 ± 0.361
5.882SerLeu: 5.882 ± 0.279
1.527SerMet: 1.527 ± 0.195
3.902SerAsn: 3.902 ± 0.257
1.885SerPro: 1.885 ± 0.224
2.224SerGln: 2.224 ± 0.168
2.338SerArg: 2.338 ± 0.186
4.807SerSer: 4.807 ± 0.315
3.77SerThr: 3.77 ± 0.295
4.034SerVal: 4.034 ± 0.292
0.716SerTrp: 0.716 ± 0.107
2.771SerTyr: 2.771 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
2.564ThrAla: 2.564 ± 0.235
0.641ThrCys: 0.641 ± 0.105
3.356ThrAsp: 3.356 ± 0.235
4.185ThrGlu: 4.185 ± 0.283
2.752ThrPhe: 2.752 ± 0.215
3.45ThrGly: 3.45 ± 0.279
1.018ThrHis: 1.018 ± 0.137
4.43ThrIle: 4.43 ± 0.308
4.637ThrLys: 4.637 ± 0.295
4.694ThrLeu: 4.694 ± 0.328
1.112ThrMet: 1.112 ± 0.129
3.073ThrAsn: 3.073 ± 0.261
2.583ThrPro: 2.583 ± 0.271
2.055ThrGln: 2.055 ± 0.168
2.47ThrArg: 2.47 ± 0.22
3.94ThrSer: 3.94 ± 0.267
2.564ThrThr: 2.564 ± 0.247
3.469ThrVal: 3.469 ± 0.296
0.829ThrTrp: 0.829 ± 0.115
2.243ThrTyr: 2.243 ± 0.224
0.0ThrXaa: 0.0 ± 0.0
Val
2.677ValAla: 2.677 ± 0.23
0.754ValCys: 0.754 ± 0.118
4.034ValAsp: 4.034 ± 0.255
4.242ValGlu: 4.242 ± 0.313
2.79ValPhe: 2.79 ± 0.203
2.865ValGly: 2.865 ± 0.272
0.698ValHis: 0.698 ± 0.114
4.826ValIle: 4.826 ± 0.311
5.995ValLys: 5.995 ± 0.424
5.241ValLeu: 5.241 ± 0.331
1.093ValMet: 1.093 ± 0.147
3.582ValAsn: 3.582 ± 0.284
2.017ValPro: 2.017 ± 0.191
2.13ValGln: 2.13 ± 0.225
2.017ValArg: 2.017 ± 0.196
4.279ValSer: 4.279 ± 0.263
2.941ValThr: 2.941 ± 0.205
3.751ValVal: 3.751 ± 0.281
0.811ValTrp: 0.811 ± 0.123
3.28ValTyr: 3.28 ± 0.267
0.0ValXaa: 0.0 ± 0.0
Trp
0.829TrpAla: 0.829 ± 0.11
0.17TrpCys: 0.17 ± 0.061
0.999TrpAsp: 0.999 ± 0.129
0.792TrpGlu: 0.792 ± 0.118
0.415TrpPhe: 0.415 ± 0.085
0.547TrpGly: 0.547 ± 0.092
0.302TrpHis: 0.302 ± 0.081
0.943TrpIle: 0.943 ± 0.143
1.037TrpLys: 1.037 ± 0.14
0.905TrpLeu: 0.905 ± 0.132
0.434TrpMet: 0.434 ± 0.078
0.811TrpAsn: 0.811 ± 0.124
0.377TrpPro: 0.377 ± 0.09
0.377TrpGln: 0.377 ± 0.086
0.283TrpArg: 0.283 ± 0.06
0.773TrpSer: 0.773 ± 0.127
0.698TrpThr: 0.698 ± 0.114
1.056TrpVal: 1.056 ± 0.165
0.094TrpTrp: 0.094 ± 0.038
0.679TrpTyr: 0.679 ± 0.108
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.338TyrAla: 2.338 ± 0.178
0.528TyrCys: 0.528 ± 0.092
3.224TyrAsp: 3.224 ± 0.243
3.242TyrGlu: 3.242 ± 0.272
2.319TyrPhe: 2.319 ± 0.217
2.809TyrGly: 2.809 ± 0.225
0.848TyrHis: 0.848 ± 0.148
3.563TyrIle: 3.563 ± 0.233
4.26TyrLys: 4.26 ± 0.262
3.186TyrLeu: 3.186 ± 0.235
0.999TyrMet: 0.999 ± 0.133
3.205TyrAsn: 3.205 ± 0.256
1.678TyrPro: 1.678 ± 0.155
1.338TyrGln: 1.338 ± 0.132
1.942TyrArg: 1.942 ± 0.179
3.205TyrSer: 3.205 ± 0.297
2.922TyrThr: 2.922 ± 0.252
2.677TyrVal: 2.677 ± 0.24
0.452TyrTrp: 0.452 ± 0.09
2.017TyrTyr: 2.017 ± 0.224
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 270 proteins (53047 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski