Microbispora triticiradicis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Microbispora

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372P3L4|A0A372P3L4_9ACTN Argininosuccinate synthase OS=Microbispora triticiradicis OX=2200763 GN=argG PE=3 SV=1
MM1 pKa = 6.49TTATYY6 pKa = 8.66TVSGMTCGHH15 pKa = 6.41CVSSVKK21 pKa = 10.74EE22 pKa = 3.95EE23 pKa = 3.98VGEE26 pKa = 4.22VTGVTGVEE34 pKa = 3.59VDD36 pKa = 3.9LATGLLTVEE45 pKa = 4.72SDD47 pKa = 3.62NPLDD51 pKa = 3.66TATIRR56 pKa = 11.84AAVEE60 pKa = 3.53EE61 pKa = 4.21AGYY64 pKa = 10.67EE65 pKa = 4.44VVDD68 pKa = 4.18HH69 pKa = 6.65PP70 pKa = 5.15

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372P4L9|A0A372P4L9_9ACTN Uncharacterized protein OS=Microbispora triticiradicis OX=2200763 GN=DI270_028565 PE=4 SV=1
MM1 pKa = 6.96STSAPPAPRR10 pKa = 11.84RR11 pKa = 11.84PRR13 pKa = 11.84TRR15 pKa = 11.84PPPPLPRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84PLLPRR30 pKa = 11.84AMPRR34 pKa = 11.84QSAPRR39 pKa = 11.84RR40 pKa = 11.84LPGPRR45 pKa = 11.84RR46 pKa = 11.84LPGPRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84SRR57 pKa = 11.84RR58 pKa = 11.84LSPQPVRR65 pKa = 11.84CSTRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84PP73 pKa = 3.08

Molecular weight: 8.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6852

0

6852

2296812

29

3602

335.2

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.521 ± 0.046

0.807 ± 0.008

5.829 ± 0.026

5.567 ± 0.036

2.751 ± 0.015

9.691 ± 0.031

2.176 ± 0.014

3.273 ± 0.019

1.818 ± 0.021

10.393 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.749 ± 0.011

1.788 ± 0.019

6.206 ± 0.025

2.489 ± 0.019

8.527 ± 0.032

5.038 ± 0.025

5.84 ± 0.024

8.839 ± 0.026

1.561 ± 0.013

2.137 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski