Campylobacter ureolyticus ACS-301-V-Sch3b
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1686 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S3XHT9|S3XHT9_9PROT Homoserine kinase OS=Campylobacter ureolyticus ACS-301-V-Sch3b OX=883165 GN=thrB PE=3 SV=1
MM1 pKa = 7.6 TYY3 pKa = 10.93 DD4 pKa = 3.58 EE5 pKa = 5.75 LEE7 pKa = 4.27 LEE9 pKa = 4.57 AYY11 pKa = 10.17 LLEE14 pKa = 4.74 ILEE17 pKa = 4.4 EE18 pKa = 4.17 YY19 pKa = 11.18 KK20 pKa = 11.18 NFDD23 pKa = 3.67 NMDD26 pKa = 3.85 DD27 pKa = 3.83 EE28 pKa = 4.6 EE29 pKa = 5.91 LYY31 pKa = 11.37 EE32 pKa = 4.35 LMSNVASYY40 pKa = 10.78 MDD42 pKa = 4.6 DD43 pKa = 3.92 DD44 pKa = 4.24 YY45 pKa = 12.0 EE46 pKa = 3.96 EE47 pKa = 5.36 AYY49 pKa = 10.21 EE50 pKa = 4.11 YY51 pKa = 9.75 VTQFGPIDD59 pKa = 3.71 KK60 pKa = 10.57 KK61 pKa = 11.02 RR62 pKa = 11.84 ILALDD67 pKa = 3.92 LPRR70 pKa = 5.85
Molecular weight: 8.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.465
ProMoST 3.808
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.376
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.91
Thurlkill 3.516
EMBOSS 3.567
Sillero 3.77
Patrickios 0.477
IPC_peptide 3.605
IPC2_peptide 3.745
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|S3XDW3|S3XDW3_9PROT Ct1132 family CRISPR-associated protein OS=Campylobacter ureolyticus ACS-301-V-Sch3b OX=883165 GN=HMPREF9309_01443 PE=4 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPHH8 pKa = 6.08 NAPKK12 pKa = 10.33 KK13 pKa = 7.7 RR14 pKa = 11.84 THH16 pKa = 6.0 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 10.18 SKK25 pKa = 10.57 NGRR28 pKa = 11.84 KK29 pKa = 9.22 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.62 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1686
0
1686
510434
29
2336
302.7
34.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.124 ± 0.054
1.054 ± 0.025
5.849 ± 0.049
6.653 ± 0.067
5.923 ± 0.063
5.807 ± 0.059
1.337 ± 0.023
9.253 ± 0.06
10.245 ± 0.066
9.83 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.237 ± 0.026
6.812 ± 0.066
2.7 ± 0.031
2.291 ± 0.028
2.922 ± 0.037
6.618 ± 0.047
4.345 ± 0.039
5.598 ± 0.052
0.637 ± 0.018
3.765 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here