Tremella mesenterica (strain ATCC 24925 / CBS 8224 / DSM 1558 / NBRC 9311 / NRRL Y-6157 / RJB 2259-6 / UBC 559-6) (Jelly fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes; Tremellales; Tremellaceae; Tremella; Tremella mesenterica

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1453 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7SD52|R7SD52_TREMS Pyridoxal 5'-phosphate synthase OS=Tremella mesenterica (strain ATCC 24925 / CBS 8224 / DSM 1558 / NBRC 9311 / NRRL Y-6157 / RJB 2259-6 / UBC 559-6) OX=578456 GN=TREMEDRAFT_70272 PE=3 SV=1
MM1 pKa = 7.85SSDD4 pKa = 3.56QPKK7 pKa = 8.09TTSQDD12 pKa = 3.47EE13 pKa = 4.3ILSFPFPTNADD24 pKa = 3.31LSLPGPSQFNTTLPSDD40 pKa = 4.09PFSMHH45 pKa = 6.87GLPPTISAEE54 pKa = 4.02FDD56 pKa = 3.8SNPIDD61 pKa = 3.44MDD63 pKa = 4.14SPSMSFLNNMNFGDD77 pKa = 4.63NPDD80 pKa = 3.63QGSGTGFNMNQYY92 pKa = 10.12EE93 pKa = 4.45MPSPNSFFPFGFSGGQSGTGNHH115 pKa = 5.83LQVGQGGVGNNFQVGQSGIPNSFGQNQNGYY145 pKa = 9.56PYY147 pKa = 10.03GQNNTGIPFGDD158 pKa = 3.43GTGFIPQTGRR168 pKa = 11.84TPGDD172 pKa = 3.37TYY174 pKa = 11.49GYY176 pKa = 9.51GQNLNWDD183 pKa = 3.92TPGNPIGRR191 pKa = 11.84GVDD194 pKa = 3.17VGAGFTSGTGGINEE208 pKa = 4.54GGGGSTGFTPP218 pKa = 4.95

Molecular weight: 22.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7SC37|R7SC37_TREMS MFS domain-containing protein OS=Tremella mesenterica (strain ATCC 24925 / CBS 8224 / DSM 1558 / NBRC 9311 / NRRL Y-6157 / RJB 2259-6 / UBC 559-6) OX=578456 GN=TREMEDRAFT_34999 PE=4 SV=1
PP1 pKa = 6.32TQSSLAKK8 pKa = 9.93VRR10 pKa = 11.84EE11 pKa = 4.24KK12 pKa = 10.51FVPPPTRR19 pKa = 11.84PSPAMSRR26 pKa = 11.84PATAMSRR33 pKa = 11.84PPTAMSRR40 pKa = 11.84PATAMSRR47 pKa = 11.84PPTAMSRR54 pKa = 11.84PATAMSRR61 pKa = 11.84PPTAMSRR68 pKa = 11.84SSTALSRR75 pKa = 11.84PPTAMSRR82 pKa = 11.84PATAMSRR89 pKa = 11.84PAA91 pKa = 3.71

Molecular weight: 9.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1453

0

1453

621631

51

3440

427.8

47.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.767 ± 0.076

1.197 ± 0.029

5.608 ± 0.051

6.582 ± 0.068

3.21 ± 0.034

6.905 ± 0.063

2.51 ± 0.038

4.788 ± 0.047

4.831 ± 0.058

8.982 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.187 ± 0.025

3.518 ± 0.035

6.916 ± 0.093

3.873 ± 0.044

6.506 ± 0.059

9.366 ± 0.087

6.338 ± 0.051

6.097 ± 0.044

1.447 ± 0.023

2.37 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski