Anaerotignum neopropionicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerotignum

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2804 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A136WEV4|A0A136WEV4_9FIRM High-affinity zinc uptake system ATP-binding protein ZnuC OS=Anaerotignum neopropionicum OX=36847 GN=znuC PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.6QMLALILTALLLCGMLAPSVFAEE25 pKa = 4.5VPPADD30 pKa = 3.98TEE32 pKa = 4.53TNDD35 pKa = 3.42TVMDD39 pKa = 3.82SHH41 pKa = 8.64SDD43 pKa = 3.16ADD45 pKa = 3.73TDD47 pKa = 5.09DD48 pKa = 5.32NIDD51 pKa = 3.99TITPSDD57 pKa = 3.55TGTPTDD63 pKa = 3.79TEE65 pKa = 4.44EE66 pKa = 5.53PPDD69 pKa = 3.65TDD71 pKa = 4.83EE72 pKa = 5.99NDD74 pKa = 4.08TPDD77 pKa = 3.56EE78 pKa = 4.43TQDD81 pKa = 3.14SSLPQDD87 pKa = 3.75FDD89 pKa = 3.8YY90 pKa = 10.93TDD92 pKa = 4.42SIISYY97 pKa = 7.33TIPDD101 pKa = 3.75AFTDD105 pKa = 3.87TFEE108 pKa = 4.57MDD110 pKa = 4.07CEE112 pKa = 4.3DD113 pKa = 3.38TSFTQLHH120 pKa = 6.0NALDD124 pKa = 3.98SVGISGTAKK133 pKa = 10.46KK134 pKa = 10.5DD135 pKa = 3.56DD136 pKa = 4.05TSSSLALSVIWDD148 pKa = 4.46FSTVDD153 pKa = 3.18AEE155 pKa = 4.82TPGSYY160 pKa = 10.18SAVGCISIPHH170 pKa = 6.1GAILADD176 pKa = 3.85GLEE179 pKa = 4.4STLSISVQVVAPMLAMSPGAITLTSFDD206 pKa = 3.4EE207 pKa = 4.74PYY209 pKa = 9.6RR210 pKa = 11.84TDD212 pKa = 3.2AVAFAVNTPQEE223 pKa = 4.33VLDD226 pKa = 4.3SWFADD231 pKa = 3.77CVAGFSGYY239 pKa = 10.53DD240 pKa = 3.27ADD242 pKa = 3.98GNYY245 pKa = 10.26YY246 pKa = 10.81DD247 pKa = 5.02LVSGAWSLDD256 pKa = 3.3AVDD259 pKa = 3.8TSTVGVYY266 pKa = 9.45YY267 pKa = 11.01VSTTPNLGTEE277 pKa = 4.08YY278 pKa = 10.21TLAEE282 pKa = 4.57GVSLPRR288 pKa = 11.84QLCAVSIQTPGEE300 pKa = 3.81PDD302 pKa = 3.25INCCVAGRR310 pKa = 11.84GFLHH314 pKa = 6.32FPWVISAVQQEE325 pKa = 4.25QLDD328 pKa = 4.36DD329 pKa = 3.74FTVWLRR335 pKa = 11.84QDD337 pKa = 3.51DD338 pKa = 4.83GEE340 pKa = 4.24WSSLSDD346 pKa = 3.45GFLLVSDD353 pKa = 5.07GLQLSQWIFISGSTYY368 pKa = 8.52EE369 pKa = 4.82LKK371 pKa = 9.43VTYY374 pKa = 9.81PGGQTGVLSFQFDD387 pKa = 4.13GEE389 pKa = 4.21LSIIDD394 pKa = 3.78YY395 pKa = 11.09SGGDD399 pKa = 3.41RR400 pKa = 11.84DD401 pKa = 4.62GGDD404 pKa = 3.58TNGSDD409 pKa = 3.68TGSNTQPAPTTPHH422 pKa = 6.35TPNNPQNKK430 pKa = 9.02GSHH433 pKa = 5.72NSVKK437 pKa = 9.22EE438 pKa = 4.07TLVPPPVPEE447 pKa = 4.08EE448 pKa = 3.85ATTQIPVPPTQSTPEE463 pKa = 3.85YY464 pKa = 8.58SQAAFSHH471 pKa = 5.32EE472 pKa = 4.27TEE474 pKa = 4.26GTVEE478 pKa = 4.07GTEE481 pKa = 3.9TCMAFQQPGTALTVMNPVPNKK502 pKa = 9.94IDD504 pKa = 3.62TQQEE508 pKa = 4.48TVFQSQEE515 pKa = 3.56PASPVFEE522 pKa = 4.88SYY524 pKa = 11.29SPTQTEE530 pKa = 3.82ISAMRR535 pKa = 11.84LQDD538 pKa = 3.59LCADD542 pKa = 3.96GEE544 pKa = 4.95SVVFGSGNLTVSIPSSLLLALNLSDD569 pKa = 5.56SDD571 pKa = 4.14TLTVTLTQPKK581 pKa = 10.27SNEE584 pKa = 3.42ILLAVAASGKK594 pKa = 10.5SVMEE598 pKa = 3.89LAGTVLRR605 pKa = 11.84LRR607 pKa = 11.84YY608 pKa = 8.81MPLWEE613 pKa = 4.22NSEE616 pKa = 3.79ITVGNEE622 pKa = 3.29AGEE625 pKa = 4.62KK626 pKa = 8.77ITDD629 pKa = 3.16ISYY632 pKa = 11.29DD633 pKa = 3.82DD634 pKa = 4.39EE635 pKa = 4.76LLCFAADD642 pKa = 3.55MPGTYY647 pKa = 9.64TISEE651 pKa = 4.29LANAEE656 pKa = 4.17KK657 pKa = 9.09THH659 pKa = 6.47KK660 pKa = 10.32SVSPLLPASMGLMLAGGGIAYY681 pKa = 9.06FRR683 pKa = 11.84RR684 pKa = 11.84KK685 pKa = 9.18HH686 pKa = 5.89HH687 pKa = 6.02GG688 pKa = 3.18

Molecular weight: 73.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A136WHZ4|A0A136WHZ4_9FIRM Uncharacterized protein OS=Anaerotignum neopropionicum OX=36847 GN=CLNEO_01590 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.93KK9 pKa = 7.63KK10 pKa = 9.62QRR12 pKa = 11.84SRR14 pKa = 11.84EE15 pKa = 3.67HH16 pKa = 6.32GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MLTSNGRR28 pKa = 11.84KK29 pKa = 9.11VLAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.23KK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2804

0

2804

838418

30

2151

299.0

33.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.562 ± 0.052

1.404 ± 0.019

5.214 ± 0.036

7.478 ± 0.054

4.464 ± 0.04

7.227 ± 0.05

1.626 ± 0.019

7.814 ± 0.045

7.1 ± 0.04

9.202 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.073 ± 0.023

4.585 ± 0.031

3.263 ± 0.029

3.312 ± 0.027

3.921 ± 0.035

5.786 ± 0.04

5.546 ± 0.055

6.856 ± 0.036

0.848 ± 0.016

3.719 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski