Avian paramyxovirus 15
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6R4X6|A0A1W6R4X6_9MONO Nucleocapsid OS=Avian paramyxovirus 15 OX=1983777 GN=NP PE=3 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.32 FKK4 pKa = 10.59 SDD6 pKa = 3.85 EE7 pKa = 4.6 EE8 pKa = 4.66 INDD11 pKa = 4.12 LLNLSSSVISEE22 pKa = 4.02 LQRR25 pKa = 11.84 AEE27 pKa = 4.3 LKK29 pKa = 10.66 PPQTLGKK36 pKa = 8.76 PQVPSGNTTSLISLWEE52 pKa = 4.06 AEE54 pKa = 4.25 GAPEE58 pKa = 4.14 NKK60 pKa = 9.73 LGRR63 pKa = 11.84 GNTGTATDD71 pKa = 3.91 TEE73 pKa = 4.82 GEE75 pKa = 3.89 QDD77 pKa = 3.49 QQIVQTEE84 pKa = 4.12 IEE86 pKa = 4.22 QTHH89 pKa = 6.15 TEE91 pKa = 4.11 DD92 pKa = 3.48 TCPKK96 pKa = 9.38 EE97 pKa = 4.2 IPVDD101 pKa = 3.68 NPGSFTQEE109 pKa = 3.24 SDD111 pKa = 3.74 LDD113 pKa = 3.72 KK114 pKa = 10.74 TLKK117 pKa = 10.71 KK118 pKa = 10.62 LEE120 pKa = 4.02 QRR122 pKa = 11.84 NSNLLKK128 pKa = 10.15 KK129 pKa = 9.03 TDD131 pKa = 3.73 SPDD134 pKa = 3.1 AGTTFKK140 pKa = 10.84 KK141 pKa = 10.52 GGQIAPKK148 pKa = 9.37 HH149 pKa = 5.49 QPLPSQVIEE158 pKa = 4.51 GNLPPQDD165 pKa = 3.94 CRR167 pKa = 11.84 QSRR170 pKa = 11.84 PSRR173 pKa = 11.84 TDD175 pKa = 2.66 ASQRR179 pKa = 11.84 PSTTPKK185 pKa = 9.69 GAHH188 pKa = 6.18 PQLHH192 pKa = 6.78 PFQDD196 pKa = 3.59 IEE198 pKa = 4.56 EE199 pKa = 4.36 NTQSVPLDD207 pKa = 3.74 HH208 pKa = 7.16 PLKK211 pKa = 10.31 LLVGATPDD219 pKa = 3.18 VHH221 pKa = 7.85 QFEE224 pKa = 4.58 QSQGEE229 pKa = 4.32 NGAHH233 pKa = 5.65 VGNVLEE239 pKa = 4.37 SASFAEE245 pKa = 4.41 MTLSVLNEE253 pKa = 3.51 VLIRR257 pKa = 11.84 VARR260 pKa = 11.84 MEE262 pKa = 4.15 EE263 pKa = 4.17 KK264 pKa = 10.61 INDD267 pKa = 3.55 ILKK270 pKa = 9.19 TNSTIPLIRR279 pKa = 11.84 NDD281 pKa = 3.0 ISQLKK286 pKa = 8.45 ATTALLSTQMASVQVLDD303 pKa = 4.39 PGNAGFKK310 pKa = 10.51 SLSEE314 pKa = 3.99 MKK316 pKa = 10.34 AASKK320 pKa = 9.74 PAIIAISGPGDD331 pKa = 3.46 MDD333 pKa = 4.27 AVPIQDD339 pKa = 3.67 KK340 pKa = 11.14 LLVKK344 pKa = 10.49 DD345 pKa = 3.51 VLGRR349 pKa = 11.84 PISAEE354 pKa = 3.68 RR355 pKa = 11.84 NIQAKK360 pKa = 7.11 EE361 pKa = 4.25 TPDD364 pKa = 3.59 SVITQSDD371 pKa = 3.06 KK372 pKa = 11.57 DD373 pKa = 4.29 AIQSLIDD380 pKa = 3.59 TLVEE384 pKa = 4.14 DD385 pKa = 3.83 TSKK388 pKa = 10.59 QARR391 pKa = 11.84 LKK393 pKa = 10.37 AQLEE397 pKa = 4.3 RR398 pKa = 11.84 VQDD401 pKa = 3.66 KK402 pKa = 10.98 PGLLKK407 pKa = 10.66 LKK409 pKa = 10.38 RR410 pKa = 11.84 LIYY413 pKa = 10.29 NAA415 pKa = 4.23
Molecular weight: 45.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.136
IPC2_protein 5.054
IPC_protein 5.003
Toseland 4.914
ProMoST 5.13
Dawson 4.978
Bjellqvist 5.105
Wikipedia 4.838
Rodwell 4.889
Grimsley 4.838
Solomon 4.978
Lehninger 4.927
Nozaki 5.092
DTASelect 5.245
Thurlkill 4.914
EMBOSS 4.876
Sillero 5.156
Patrickios 4.609
IPC_peptide 4.978
IPC2_peptide 5.156
IPC2.peptide.svr19 5.139
Protein with the highest isoelectric point:
>tr|A0A1W6R4T0|A0A1W6R4T0_9MONO Fusion glycoprotein F0 OS=Avian paramyxovirus 15 OX=1983777 GN=F PE=3 SV=1
MM1 pKa = 7.85 ASTSIPLHH9 pKa = 6.37 INQSDD14 pKa = 3.88 PSSEE18 pKa = 4.02 LLSFPLIFTNNDD30 pKa = 3.22 SGSKK34 pKa = 8.52 TLQPQVRR41 pKa = 11.84 FSQLGDD47 pKa = 3.2 IQGGKK52 pKa = 10.26 DD53 pKa = 3.05 EE54 pKa = 4.75 TVFITLYY61 pKa = 11.1 GFIQTEE67 pKa = 4.09 QDD69 pKa = 4.11 SLDD72 pKa = 3.63 LTHH75 pKa = 6.4 NQSTFQVLGQTSKK88 pKa = 11.11 PDD90 pKa = 3.88 TITAACIPLGATTLRR105 pKa = 11.84 STITRR110 pKa = 11.84 MAEE113 pKa = 3.68 EE114 pKa = 4.12 SLEE117 pKa = 4.02 LLILVKK123 pKa = 10.51 KK124 pKa = 10.42 SAVSSEE130 pKa = 3.73 KK131 pKa = 10.68 LAVSFINIPPSLAGILVIRR150 pKa = 11.84 AGGFILSAEE159 pKa = 4.6 EE160 pKa = 4.22 YY161 pKa = 10.08 VKK163 pKa = 11.03 SPSKK167 pKa = 10.63 LQAGYY172 pKa = 9.62 QYY174 pKa = 11.13 KK175 pKa = 10.17 FKK177 pKa = 10.59 PVFITCTRR185 pKa = 11.84 IFKK188 pKa = 10.58 GKK190 pKa = 9.8 LYY192 pKa = 10.26 KK193 pKa = 10.29 VPKK196 pKa = 8.6 SMHH199 pKa = 6.19 YY200 pKa = 9.7 IASEE204 pKa = 3.99 LLYY207 pKa = 10.79 KK208 pKa = 10.57 AVLEE212 pKa = 4.33 IQFQLDD218 pKa = 3.73 IKK220 pKa = 10.24 PDD222 pKa = 3.49 HH223 pKa = 6.98 PQTKK227 pKa = 8.63 MLKK230 pKa = 10.11 KK231 pKa = 9.99 QDD233 pKa = 3.75 TNEE236 pKa = 3.76 GPEE239 pKa = 4.01 YY240 pKa = 10.83 YY241 pKa = 10.79 GFVWFHH247 pKa = 6.5 LLNFKK252 pKa = 9.06 KK253 pKa = 7.4 TTARR257 pKa = 11.84 GEE259 pKa = 3.75 IRR261 pKa = 11.84 TLEE264 pKa = 4.39 KK265 pKa = 10.51 ISDD268 pKa = 4.2 KK269 pKa = 10.51 IRR271 pKa = 11.84 AMGIKK276 pKa = 10.13 VSLYY280 pKa = 10.38 DD281 pKa = 3.25 LWGPTILAEE290 pKa = 4.02 ITGKK294 pKa = 9.9 KK295 pKa = 9.38 SKK297 pKa = 9.98 YY298 pKa = 8.52 AQGFFSLNGCACLPVARR315 pKa = 11.84 ASPEE319 pKa = 3.55 IAKK322 pKa = 9.59 LIWSCSTRR330 pKa = 11.84 IKK332 pKa = 10.52 SATIIVQSSDD342 pKa = 2.76 KK343 pKa = 10.86 RR344 pKa = 11.84 GLLNSEE350 pKa = 4.11 DD351 pKa = 4.61 LEE353 pKa = 4.47 IKK355 pKa = 10.34 GAVGVSPRR363 pKa = 11.84 KK364 pKa = 9.76 LGSYY368 pKa = 10.68 SLFKK372 pKa = 10.72 KK373 pKa = 9.92 HH374 pKa = 6.4
Molecular weight: 41.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.778
IPC2_protein 8.682
IPC_protein 8.551
Toseland 9.385
ProMoST 9.107
Dawson 9.633
Bjellqvist 9.355
Wikipedia 9.78
Rodwell 10.014
Grimsley 9.692
Solomon 9.677
Lehninger 9.633
Nozaki 9.516
DTASelect 9.297
Thurlkill 9.502
EMBOSS 9.809
Sillero 9.589
Patrickios 5.08
IPC_peptide 9.663
IPC2_peptide 7.892
IPC2.peptide.svr19 7.824
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4581
374
2216
763.5
85.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.112 ± 0.611
1.877 ± 0.234
4.824 ± 0.359
5.523 ± 0.35
3.209 ± 0.377
5.152 ± 0.337
1.877 ± 0.333
8.557 ± 0.567
5.283 ± 0.552
11.111 ± 0.477
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.877 ± 0.286
5.108 ± 0.44
4.475 ± 0.499
4.431 ± 0.429
4.671 ± 0.533
9.146 ± 0.179
6.702 ± 0.406
5.763 ± 0.424
1.026 ± 0.187
3.274 ± 0.416
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here