Thermus phage phiMa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8HYZ0|A0A3Q8HYZ0_9CAUD Uncharacterized protein OS=Thermus phage phiMa OX=2301537 GN=phiMa_01 PE=4 SV=1
MM1 pKa = 7.84TIPEE5 pKa = 4.22AQVLLRR11 pKa = 11.84LHH13 pKa = 6.66AMSLGRR19 pKa = 11.84ADD21 pKa = 4.37PLEE24 pKa = 4.05AWRR27 pKa = 11.84EE28 pKa = 3.95EE29 pKa = 3.69QRR31 pKa = 11.84FASEE35 pKa = 5.95LEE37 pKa = 4.29AQDD40 pKa = 3.77AWDD43 pKa = 3.67AALPGGDD50 pKa = 3.77GYY52 pKa = 11.13EE53 pKa = 4.63GPRR56 pKa = 11.84GVYY59 pKa = 9.78PYY61 pKa = 11.34GRR63 pKa = 11.84GG64 pKa = 3.26

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A451ED85|A0A451ED85_9CAUD Uncharacterized protein OS=Thermus phage phiMa OX=2301537 GN=phiMa_21 PE=4 SV=1
MM1 pKa = 7.8DD2 pKa = 5.02RR3 pKa = 11.84PVSLHH8 pKa = 5.56EE9 pKa = 4.49LFPRR13 pKa = 11.84MADD16 pKa = 3.37LLEE19 pKa = 4.06ARR21 pKa = 11.84ARR23 pKa = 11.84HH24 pKa = 5.09LQEE27 pKa = 5.15AATLDD32 pKa = 3.61QEE34 pKa = 4.7LARR37 pKa = 11.84LMRR40 pKa = 11.84QAPALMEE47 pKa = 3.88RR48 pKa = 11.84HH49 pKa = 5.88GEE51 pKa = 4.08GGTLSLHH58 pKa = 5.88EE59 pKa = 4.28VASFLGISYY68 pKa = 8.24STAYY72 pKa = 10.34QMARR76 pKa = 11.84SGAIPVFRR84 pKa = 11.84VGRR87 pKa = 11.84RR88 pKa = 11.84YY89 pKa = 9.51RR90 pKa = 11.84VPRR93 pKa = 11.84SALKK97 pKa = 10.55ALEE100 pKa = 4.3AGQRR104 pKa = 11.84PAA106 pKa = 4.54

Molecular weight: 11.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

16690

33

1571

273.6

30.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.432 ± 0.347

0.503 ± 0.095

5.009 ± 0.366

8.31 ± 0.478

2.654 ± 0.143

8.886 ± 0.38

2.451 ± 0.305

3.355 ± 0.183

2.906 ± 0.349

10.437 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.118

1.75 ± 0.105

6.842 ± 0.34

3.235 ± 0.181

9.401 ± 0.393

4.026 ± 0.202

4.26 ± 0.262

7.154 ± 0.295

1.989 ± 0.17

3.481 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski