Amino acid dipepetide frequency for Thermus phage phiMa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.007AlaAla: 10.007 ± 0.921
0.719AlaCys: 0.719 ± 0.225
4.194AlaAsp: 4.194 ± 0.746
7.67AlaGlu: 7.67 ± 0.929
4.134AlaPhe: 4.134 ± 0.443
8.269AlaGly: 8.269 ± 1.025
3.176AlaHis: 3.176 ± 0.577
3.415AlaIle: 3.415 ± 0.518
3.775AlaLys: 3.775 ± 0.7
15.879AlaLeu: 15.879 ± 1.307
2.037AlaMet: 2.037 ± 0.319
1.738AlaAsn: 1.738 ± 0.294
6.711AlaPro: 6.711 ± 0.739
6.172AlaGln: 6.172 ± 0.7
11.325AlaArg: 11.325 ± 1.222
4.494AlaSer: 4.494 ± 0.565
4.554AlaThr: 4.554 ± 0.572
8.928AlaVal: 8.928 ± 0.765
2.756AlaTrp: 2.756 ± 0.454
4.254AlaTyr: 4.254 ± 0.535
0.06AlaXaa: 0.06 ± 0.052
Cys
0.479CysAla: 0.479 ± 0.17
0.12CysCys: 0.12 ± 0.088
0.12CysAsp: 0.12 ± 0.091
0.419CysGlu: 0.419 ± 0.139
0.12CysPhe: 0.12 ± 0.077
0.479CysGly: 0.479 ± 0.187
0.06CysHis: 0.06 ± 0.057
0.18CysIle: 0.18 ± 0.093
0.18CysLys: 0.18 ± 0.11
0.419CysLeu: 0.419 ± 0.169
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.719CysPro: 0.719 ± 0.275
0.36CysGln: 0.36 ± 0.135
0.719CysArg: 0.719 ± 0.255
0.12CysSer: 0.12 ± 0.088
0.0CysThr: 0.0 ± 0.0
0.24CysVal: 0.24 ± 0.1
0.06CysTrp: 0.06 ± 0.071
0.24CysTyr: 0.24 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
5.513AspAla: 5.513 ± 0.61
0.12AspCys: 0.12 ± 0.078
1.977AspAsp: 1.977 ± 0.359
3.116AspGlu: 3.116 ± 0.483
1.258AspPhe: 1.258 ± 0.274
4.194AspGly: 4.194 ± 0.623
1.738AspHis: 1.738 ± 0.571
1.498AspIle: 1.498 ± 0.333
0.539AspLys: 0.539 ± 0.202
6.771AspLeu: 6.771 ± 0.729
0.419AspMet: 0.419 ± 0.167
0.18AspAsn: 0.18 ± 0.106
6.351AspPro: 6.351 ± 0.612
1.378AspGln: 1.378 ± 0.407
4.015AspArg: 4.015 ± 0.503
1.977AspSer: 1.977 ± 0.651
2.636AspThr: 2.636 ± 1.265
3.595AspVal: 3.595 ± 0.403
1.438AspTrp: 1.438 ± 0.282
1.378AspTyr: 1.378 ± 0.275
0.0AspXaa: 0.0 ± 0.0
Glu
15.28GluAla: 15.28 ± 1.442
0.18GluCys: 0.18 ± 0.094
5.093GluAsp: 5.093 ± 0.834
8.569GluGlu: 8.569 ± 1.022
1.618GluPhe: 1.618 ± 0.346
7.79GluGly: 7.79 ± 0.831
1.438GluHis: 1.438 ± 0.296
2.157GluIle: 2.157 ± 0.296
1.977GluLys: 1.977 ± 0.48
7.43GluLeu: 7.43 ± 0.959
1.318GluMet: 1.318 ± 0.291
0.959GluAsn: 0.959 ± 0.203
3.955GluPro: 3.955 ± 0.492
1.438GluGln: 1.438 ± 0.296
7.071GluArg: 7.071 ± 0.92
1.858GluSer: 1.858 ± 0.265
2.636GluThr: 2.636 ± 0.443
7.849GluVal: 7.849 ± 0.964
2.217GluTrp: 2.217 ± 0.375
2.277GluTyr: 2.277 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
2.876PheAla: 2.876 ± 0.414
0.12PheCys: 0.12 ± 0.085
0.959PheAsp: 0.959 ± 0.223
1.738PheGlu: 1.738 ± 0.332
0.779PhePhe: 0.779 ± 0.218
2.037PheGly: 2.037 ± 0.305
0.839PheHis: 0.839 ± 0.214
0.599PheIle: 0.599 ± 0.172
0.779PheLys: 0.779 ± 0.222
3.116PheLeu: 3.116 ± 0.498
0.419PheMet: 0.419 ± 0.122
0.419PheAsn: 0.419 ± 0.141
2.097PhePro: 2.097 ± 0.336
1.019PheGln: 1.019 ± 0.261
3.236PheArg: 3.236 ± 0.422
1.378PheSer: 1.378 ± 0.303
0.779PheThr: 0.779 ± 0.184
1.977PheVal: 1.977 ± 0.343
0.36PheTrp: 0.36 ± 0.15
1.019PheTyr: 1.019 ± 0.228
0.0PheXaa: 0.0 ± 0.0
Gly
9.347GlyAla: 9.347 ± 0.973
0.419GlyCys: 0.419 ± 0.195
5.513GlyAsp: 5.513 ± 0.983
7.25GlyGlu: 7.25 ± 0.645
2.756GlyPhe: 2.756 ± 0.428
8.569GlyGly: 8.569 ± 1.047
1.438GlyHis: 1.438 ± 0.344
3.056GlyIle: 3.056 ± 0.47
2.097GlyLys: 2.097 ± 0.357
8.808GlyLeu: 8.808 ± 0.837
2.756GlyMet: 2.756 ± 0.323
1.498GlyAsn: 1.498 ± 0.339
4.194GlyPro: 4.194 ± 0.497
2.756GlyGln: 2.756 ± 0.409
7.43GlyArg: 7.43 ± 0.59
4.314GlySer: 4.314 ± 0.753
4.075GlyThr: 4.075 ± 0.587
7.25GlyVal: 7.25 ± 0.604
1.738GlyTrp: 1.738 ± 0.316
3.595GlyTyr: 3.595 ± 0.559
0.0GlyXaa: 0.0 ± 0.0
His
2.577HisAla: 2.577 ± 0.705
0.18HisCys: 0.18 ± 0.105
0.779HisAsp: 0.779 ± 0.187
1.258HisGlu: 1.258 ± 0.397
0.659HisPhe: 0.659 ± 0.201
2.457HisGly: 2.457 ± 0.88
0.599HisHis: 0.599 ± 0.212
0.779HisIle: 0.779 ± 0.24
0.659HisLys: 0.659 ± 0.21
3.475HisLeu: 3.475 ± 0.336
0.479HisMet: 0.479 ± 0.188
0.599HisAsn: 0.599 ± 0.187
1.917HisPro: 1.917 ± 0.409
0.899HisGln: 0.899 ± 0.228
1.318HisArg: 1.318 ± 0.363
1.678HisSer: 1.678 ± 1.001
1.318HisThr: 1.318 ± 0.322
1.558HisVal: 1.558 ± 0.291
0.719HisTrp: 0.719 ± 0.224
0.599HisTyr: 0.599 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
4.134IleAla: 4.134 ± 0.47
0.3IleCys: 0.3 ± 0.194
1.558IleAsp: 1.558 ± 0.335
2.277IleGlu: 2.277 ± 0.41
0.599IlePhe: 0.599 ± 0.151
2.577IleGly: 2.577 ± 0.255
0.539IleHis: 0.539 ± 0.183
0.959IleIle: 0.959 ± 0.272
0.839IleLys: 0.839 ± 0.217
3.715IleLeu: 3.715 ± 0.435
0.24IleMet: 0.24 ± 0.133
0.479IleAsn: 0.479 ± 0.154
2.816IlePro: 2.816 ± 0.468
1.378IleGln: 1.378 ± 0.247
4.734IleArg: 4.734 ± 0.465
0.899IleSer: 0.899 ± 0.231
2.277IleThr: 2.277 ± 0.319
1.798IleVal: 1.798 ± 0.314
0.12IleTrp: 0.12 ± 0.079
1.318IleTyr: 1.318 ± 0.297
0.0IleXaa: 0.0 ± 0.0
Lys
5.453LysAla: 5.453 ± 0.626
0.06LysCys: 0.06 ± 0.057
1.498LysAsp: 1.498 ± 0.29
2.397LysGlu: 2.397 ± 0.498
0.419LysPhe: 0.419 ± 0.151
2.517LysGly: 2.517 ± 0.412
0.659LysHis: 0.659 ± 0.204
0.719LysIle: 0.719 ± 0.203
1.558LysLys: 1.558 ± 0.429
1.858LysLeu: 1.858 ± 0.366
0.3LysMet: 0.3 ± 0.14
0.839LysAsn: 0.839 ± 0.215
1.858LysPro: 1.858 ± 0.411
0.539LysGln: 0.539 ± 0.203
3.356LysArg: 3.356 ± 0.666
1.318LysSer: 1.318 ± 0.313
1.258LysThr: 1.258 ± 0.374
1.558LysVal: 1.558 ± 0.255
0.539LysTrp: 0.539 ± 0.188
0.36LysTyr: 0.36 ± 0.162
0.0LysXaa: 0.0 ± 0.0
Leu
11.385LeuAla: 11.385 ± 0.864
0.36LeuCys: 0.36 ± 0.137
5.632LeuAsp: 5.632 ± 0.505
12.523LeuGlu: 12.523 ± 0.909
2.696LeuPhe: 2.696 ± 0.386
9.467LeuGly: 9.467 ± 0.854
2.876LeuHis: 2.876 ± 0.422
3.296LeuIle: 3.296 ± 0.544
3.595LeuLys: 3.595 ± 0.557
8.748LeuLeu: 8.748 ± 0.866
2.577LeuMet: 2.577 ± 0.366
2.277LeuAsn: 2.277 ± 0.373
7.37LeuPro: 7.37 ± 0.614
3.296LeuGln: 3.296 ± 0.522
10.426LeuArg: 10.426 ± 0.866
4.614LeuSer: 4.614 ± 0.567
4.614LeuThr: 4.614 ± 0.69
3.595LeuVal: 3.595 ± 0.466
1.678LeuTrp: 1.678 ± 0.359
3.356LeuTyr: 3.356 ± 0.468
0.0LeuXaa: 0.0 ± 0.0
Met
2.636MetAla: 2.636 ± 0.373
0.06MetCys: 0.06 ± 0.064
0.959MetAsp: 0.959 ± 0.226
1.438MetGlu: 1.438 ± 0.324
0.3MetPhe: 0.3 ± 0.119
2.816MetGly: 2.816 ± 0.433
0.479MetHis: 0.479 ± 0.182
0.419MetIle: 0.419 ± 0.151
0.599MetLys: 0.599 ± 0.194
1.138MetLeu: 1.138 ± 0.187
0.3MetMet: 0.3 ± 0.19
0.12MetAsn: 0.12 ± 0.066
1.198MetPro: 1.198 ± 0.266
0.719MetGln: 0.719 ± 0.221
2.337MetArg: 2.337 ± 0.464
0.659MetSer: 0.659 ± 0.198
0.719MetThr: 0.719 ± 0.173
1.079MetVal: 1.079 ± 0.264
0.419MetTrp: 0.419 ± 0.156
0.3MetTyr: 0.3 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
1.917AsnAla: 1.917 ± 0.308
0.18AsnCys: 0.18 ± 0.135
1.138AsnAsp: 1.138 ± 0.316
0.599AsnGlu: 0.599 ± 0.194
0.3AsnPhe: 0.3 ± 0.158
1.678AsnGly: 1.678 ± 0.343
0.719AsnHis: 0.719 ± 0.192
0.719AsnIle: 0.719 ± 0.203
0.539AsnLys: 0.539 ± 0.191
2.277AsnLeu: 2.277 ± 0.395
0.18AsnMet: 0.18 ± 0.122
0.06AsnAsn: 0.06 ± 0.063
1.917AsnPro: 1.917 ± 0.362
0.36AsnGln: 0.36 ± 0.159
1.858AsnArg: 1.858 ± 0.364
0.419AsnSer: 0.419 ± 0.178
1.079AsnThr: 1.079 ± 0.336
0.959AsnVal: 0.959 ± 0.292
0.12AsnTrp: 0.12 ± 0.08
0.479AsnTyr: 0.479 ± 0.143
0.0AsnXaa: 0.0 ± 0.0
Pro
6.052ProAla: 6.052 ± 0.663
0.36ProCys: 0.36 ± 0.162
3.236ProAsp: 3.236 ± 0.464
7.55ProGlu: 7.55 ± 0.796
1.378ProPhe: 1.378 ± 0.282
6.172ProGly: 6.172 ± 0.564
1.977ProHis: 1.977 ± 0.441
2.337ProIle: 2.337 ± 0.314
2.636ProLys: 2.636 ± 0.698
5.453ProLeu: 5.453 ± 0.521
0.959ProMet: 0.959 ± 0.234
1.318ProAsn: 1.318 ± 0.312
5.632ProPro: 5.632 ± 0.682
2.217ProGln: 2.217 ± 0.376
4.853ProArg: 4.853 ± 0.672
3.775ProSer: 3.775 ± 0.457
3.595ProThr: 3.595 ± 0.815
4.973ProVal: 4.973 ± 0.551
1.438ProTrp: 1.438 ± 0.36
2.517ProTyr: 2.517 ± 0.375
0.0ProXaa: 0.0 ± 0.0
Gln
5.033GlnAla: 5.033 ± 0.688
0.18GlnCys: 0.18 ± 0.089
2.577GlnAsp: 2.577 ± 0.387
2.397GlnGlu: 2.397 ± 0.469
0.419GlnPhe: 0.419 ± 0.124
3.535GlnGly: 3.535 ± 0.438
0.479GlnHis: 0.479 ± 0.138
1.138GlnIle: 1.138 ± 0.184
1.618GlnLys: 1.618 ± 0.311
1.498GlnLeu: 1.498 ± 0.305
0.659GlnMet: 0.659 ± 0.225
1.138GlnAsn: 1.138 ± 0.212
1.318GlnPro: 1.318 ± 0.383
0.539GlnGln: 0.539 ± 0.19
3.236GlnArg: 3.236 ± 0.464
1.019GlnSer: 1.019 ± 0.305
1.438GlnThr: 1.438 ± 0.292
3.116GlnVal: 3.116 ± 0.389
0.479GlnTrp: 0.479 ± 0.155
0.539GlnTyr: 0.539 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
9.407ArgAla: 9.407 ± 0.81
0.599ArgCys: 0.599 ± 0.234
3.415ArgAsp: 3.415 ± 0.531
8.569ArgGlu: 8.569 ± 0.935
3.595ArgPhe: 3.595 ± 0.488
7.25ArgGly: 7.25 ± 0.716
1.498ArgHis: 1.498 ± 0.336
4.254ArgIle: 4.254 ± 0.519
3.475ArgLys: 3.475 ± 0.672
11.385ArgLeu: 11.385 ± 0.871
2.696ArgMet: 2.696 ± 0.365
1.618ArgAsn: 1.618 ± 0.312
5.393ArgPro: 5.393 ± 0.658
2.157ArgGln: 2.157 ± 0.357
8.509ArgArg: 8.509 ± 0.847
3.535ArgSer: 3.535 ± 0.534
3.655ArgThr: 3.655 ± 0.543
7.011ArgVal: 7.011 ± 0.663
1.258ArgTrp: 1.258 ± 0.273
4.734ArgTyr: 4.734 ± 0.53
0.0ArgXaa: 0.0 ± 0.0
Ser
3.056SerAla: 3.056 ± 0.379
0.24SerCys: 0.24 ± 0.106
2.397SerAsp: 2.397 ± 1.048
2.457SerGlu: 2.457 ± 0.392
0.779SerPhe: 0.779 ± 0.179
3.236SerGly: 3.236 ± 0.484
1.318SerHis: 1.318 ± 0.517
2.157SerIle: 2.157 ± 0.382
0.779SerLys: 0.779 ± 0.165
4.674SerLeu: 4.674 ± 0.518
0.659SerMet: 0.659 ± 0.154
0.659SerAsn: 0.659 ± 0.215
3.775SerPro: 3.775 ± 0.366
1.258SerGln: 1.258 ± 0.26
3.715SerArg: 3.715 ± 0.491
1.678SerSer: 1.678 ± 0.404
2.097SerThr: 2.097 ± 0.429
2.816SerVal: 2.816 ± 0.494
0.779SerTrp: 0.779 ± 0.204
1.738SerTyr: 1.738 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
4.794ThrAla: 4.794 ± 0.595
0.18ThrCys: 0.18 ± 0.103
1.858ThrAsp: 1.858 ± 0.456
2.157ThrGlu: 2.157 ± 0.348
1.438ThrPhe: 1.438 ± 0.291
4.614ThrGly: 4.614 ± 0.541
1.438ThrHis: 1.438 ± 0.749
1.678ThrIle: 1.678 ± 0.369
0.659ThrLys: 0.659 ± 0.276
5.093ThrLeu: 5.093 ± 0.573
0.419ThrMet: 0.419 ± 0.181
0.719ThrAsn: 0.719 ± 0.217
3.895ThrPro: 3.895 ± 0.513
1.198ThrGln: 1.198 ± 0.31
3.236ThrArg: 3.236 ± 0.352
1.798ThrSer: 1.798 ± 0.309
1.438ThrThr: 1.438 ± 0.504
3.116ThrVal: 3.116 ± 0.57
1.079ThrTrp: 1.079 ± 0.292
1.798ThrTyr: 1.798 ± 0.275
0.0ThrXaa: 0.0 ± 0.0
Val
7.49ValAla: 7.49 ± 0.682
0.479ValCys: 0.479 ± 0.242
4.314ValAsp: 4.314 ± 0.516
6.411ValGlu: 6.411 ± 0.51
1.858ValPhe: 1.858 ± 0.27
6.172ValGly: 6.172 ± 0.887
1.858ValHis: 1.858 ± 0.284
2.217ValIle: 2.217 ± 0.376
1.858ValLys: 1.858 ± 0.282
7.071ValLeu: 7.071 ± 0.699
1.378ValMet: 1.378 ± 0.288
1.858ValAsn: 1.858 ± 0.329
3.955ValPro: 3.955 ± 0.495
2.996ValGln: 2.996 ± 0.464
7.19ValArg: 7.19 ± 0.896
2.397ValSer: 2.397 ± 0.358
1.798ValThr: 1.798 ± 0.322
5.992ValVal: 5.992 ± 0.818
1.917ValTrp: 1.917 ± 0.345
2.337ValTyr: 2.337 ± 0.456
0.0ValXaa: 0.0 ± 0.0
Trp
2.277TrpAla: 2.277 ± 0.439
0.12TrpCys: 0.12 ± 0.084
1.258TrpAsp: 1.258 ± 0.333
1.678TrpGlu: 1.678 ± 0.378
0.599TrpPhe: 0.599 ± 0.183
1.798TrpGly: 1.798 ± 0.346
0.479TrpHis: 0.479 ± 0.182
0.839TrpIle: 0.839 ± 0.234
0.419TrpLys: 0.419 ± 0.147
1.738TrpLeu: 1.738 ± 0.271
0.36TrpMet: 0.36 ± 0.187
0.599TrpAsn: 0.599 ± 0.189
1.019TrpPro: 1.019 ± 0.25
0.419TrpGln: 0.419 ± 0.143
1.738TrpArg: 1.738 ± 0.328
0.719TrpSer: 0.719 ± 0.202
1.618TrpThr: 1.618 ± 0.362
1.258TrpVal: 1.258 ± 0.281
0.36TrpTrp: 0.36 ± 0.167
0.599TrpTyr: 0.599 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.614TyrAla: 4.614 ± 0.55
0.06TyrCys: 0.06 ± 0.062
1.618TyrAsp: 1.618 ± 0.325
2.636TyrGlu: 2.636 ± 0.42
0.839TyrPhe: 0.839 ± 0.212
3.236TyrGly: 3.236 ± 0.449
0.899TyrHis: 0.899 ± 0.212
1.138TyrIle: 1.138 ± 0.228
0.479TyrLys: 0.479 ± 0.174
3.835TyrLeu: 3.835 ± 0.462
0.479TyrMet: 0.479 ± 0.159
0.419TyrAsn: 0.419 ± 0.154
2.277TyrPro: 2.277 ± 0.376
1.258TyrGln: 1.258 ± 0.31
3.715TyrArg: 3.715 ± 0.472
1.618TyrSer: 1.618 ± 0.319
1.019TyrThr: 1.019 ± 0.192
2.876TyrVal: 2.876 ± 0.358
0.419TyrTrp: 0.419 ± 0.147
1.378TyrTyr: 1.378 ± 0.3
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.06XaaPro: 0.06 ± 0.052
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.419XaaXaa: 0.419 ± 0.362
Statistics based on 61 proteins (16690 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski