Sulfurihydrogenibium yellowstonense SS-5

Taxonomy: cellular organisms; Bacteria; Aquificae; Aquificae; Aquificales; Hydrogenothermaceae; Sulfurihydrogenibium; Sulfurihydrogenibium yellowstonense

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1670 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C4FID9|C4FID9_9AQUI Uncharacterized protein OS=Sulfurihydrogenibium yellowstonense SS-5 OX=432331 GN=SULYE_0324 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 6.37LIIGLIALIAIILMIRR18 pKa = 11.84KK19 pKa = 9.46VGVIEE24 pKa = 4.05PNRR27 pKa = 11.84NVASNLQPLQNPQPSPTIQNIPQNTPSQNNSNALNDD63 pKa = 3.35ITKK66 pKa = 10.56AGLIGTATGLGLIGAGMLLNRR87 pKa = 11.84NNYY90 pKa = 7.86NYY92 pKa = 10.43YY93 pKa = 10.16YY94 pKa = 9.96PRR96 pKa = 11.84EE97 pKa = 4.22PDD99 pKa = 3.07TALAEE104 pKa = 4.25HH105 pKa = 6.49LHH107 pKa = 6.05DD108 pKa = 6.52LIWHH112 pKa = 7.04DD113 pKa = 5.56DD114 pKa = 3.82CNKK117 pKa = 10.01CDD119 pKa = 3.88NDD121 pKa = 3.78VIYY124 pKa = 8.99NTFSSCDD131 pKa = 3.31DD132 pKa = 3.53TGFDD136 pKa = 4.65SSNSSCSSSWDD147 pKa = 3.45DD148 pKa = 4.2NYY150 pKa = 11.7DD151 pKa = 3.77NNHH154 pKa = 6.67HH155 pKa = 7.57DD156 pKa = 6.06DD157 pKa = 4.53GYY159 pKa = 11.68SWNSWDD165 pKa = 4.93SNDD168 pKa = 5.96DD169 pKa = 3.85GYY171 pKa = 11.99SWNSWDD177 pKa = 5.26SGDD180 pKa = 4.25DD181 pKa = 3.8SSWW184 pKa = 2.97

Molecular weight:
20.49 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C4FLK4|C4FLK4_9AQUI 3-dehydroquinate dehydratase OS=Sulfurihydrogenibium yellowstonense SS-5 OX=432331 GN=aroD PE=3 SV=1
MM1 pKa = 7.71ANMKK5 pKa = 10.3AKK7 pKa = 10.35SHH9 pKa = 6.63LSNKK13 pKa = 8.37KK14 pKa = 9.46RR15 pKa = 11.84KK16 pKa = 7.99RR17 pKa = 11.84TSGFLARR24 pKa = 11.84MKK26 pKa = 9.95TKK28 pKa = 10.54AGRR31 pKa = 11.84KK32 pKa = 7.59ILARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AKK40 pKa = 9.7GRR42 pKa = 11.84KK43 pKa = 9.06RR44 pKa = 11.84IAIKK48 pKa = 10.57

Molecular weight:
5.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1670

0

1670

435704

29

1488

260.9

29.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.656 ± 0.063

0.718 ± 0.02

5.347 ± 0.044

8.173 ± 0.076

4.997 ± 0.056

5.918 ± 0.059

1.454 ± 0.024

9.45 ± 0.062

10.038 ± 0.074

9.83 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.954 ± 0.029

5.188 ± 0.056

3.569 ± 0.037

2.597 ± 0.035

3.609 ± 0.042

5.462 ± 0.042

4.406 ± 0.041

6.481 ± 0.055

0.77 ± 0.021

4.382 ± 0.048

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski