Sulfurihydrogenibium yellowstonense SS-5
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1670 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C4FID9|C4FID9_9AQUI Uncharacterized protein OS=Sulfurihydrogenibium yellowstonense SS-5 OX=432331 GN=SULYE_0324 PE=4 SV=1
MM1 pKa = 7.44 EE2 pKa = 6.37 LIIGLIALIAIILMIRR18 pKa = 11.84 KK19 pKa = 9.46 VGVIEE24 pKa = 4.05 PNRR27 pKa = 11.84 NVASNLQPLQNPQPSPTIQNIPQNTPSQNNSNALNDD63 pKa = 3.35 ITKK66 pKa = 10.56 AGLIGTATGLGLIGAGMLLNRR87 pKa = 11.84 NNYY90 pKa = 7.86 NYY92 pKa = 10.43 YY93 pKa = 10.16 YY94 pKa = 9.96 PRR96 pKa = 11.84 EE97 pKa = 4.22 PDD99 pKa = 3.07 TALAEE104 pKa = 4.25 HH105 pKa = 6.49 LHH107 pKa = 6.05 DD108 pKa = 6.52 LIWHH112 pKa = 7.04 DD113 pKa = 5.56 DD114 pKa = 3.82 CNKK117 pKa = 10.01 CDD119 pKa = 3.88 NDD121 pKa = 3.78 VIYY124 pKa = 8.99 NTFSSCDD131 pKa = 3.31 DD132 pKa = 3.53 TGFDD136 pKa = 4.65 SSNSSCSSSWDD147 pKa = 3.45 DD148 pKa = 4.2 NYY150 pKa = 11.7 DD151 pKa = 3.77 NNHH154 pKa = 6.67 HH155 pKa = 7.57 DD156 pKa = 6.06 DD157 pKa = 4.53 GYY159 pKa = 11.68 SWNSWDD165 pKa = 4.93 SNDD168 pKa = 5.96 DD169 pKa = 3.85 GYY171 pKa = 11.99 SWNSWDD177 pKa = 5.26 SGDD180 pKa = 4.25 DD181 pKa = 3.8 SSWW184 pKa = 2.97
Molecular weight: 20.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.805
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.681
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.91
Rodwell 3.745
Grimsley 3.592
Solomon 3.923
Lehninger 3.884
Nozaki 4.05
DTASelect 4.368
Thurlkill 3.757
EMBOSS 3.923
Sillero 4.05
Patrickios 0.515
IPC_peptide 3.91
IPC2_peptide 4.012
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|C4FLK4|C4FLK4_9AQUI 3-dehydroquinate dehydratase OS=Sulfurihydrogenibium yellowstonense SS-5 OX=432331 GN=aroD PE=3 SV=1
MM1 pKa = 7.71 ANMKK5 pKa = 10.3 AKK7 pKa = 10.35 SHH9 pKa = 6.63 LSNKK13 pKa = 8.37 KK14 pKa = 9.46 RR15 pKa = 11.84 KK16 pKa = 7.99 RR17 pKa = 11.84 TSGFLARR24 pKa = 11.84 MKK26 pKa = 9.95 TKK28 pKa = 10.54 AGRR31 pKa = 11.84 KK32 pKa = 7.59 ILARR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 9.7 GRR42 pKa = 11.84 KK43 pKa = 9.06 RR44 pKa = 11.84 IAIKK48 pKa = 10.57
Molecular weight: 5.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.505
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.778
Grimsley 12.969
Solomon 13.408
Lehninger 13.305
Nozaki 12.925
DTASelect 12.91
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.501
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1670
0
1670
435704
29
1488
260.9
29.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.656 ± 0.063
0.718 ± 0.02
5.347 ± 0.044
8.173 ± 0.076
4.997 ± 0.056
5.918 ± 0.059
1.454 ± 0.024
9.45 ± 0.062
10.038 ± 0.074
9.83 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.954 ± 0.029
5.188 ± 0.056
3.569 ± 0.037
2.597 ± 0.035
3.609 ± 0.042
5.462 ± 0.042
4.406 ± 0.041
6.481 ± 0.055
0.77 ± 0.021
4.382 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here