Taro vein chlorosis virus (TAVCV)
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q5GA90|NCAP_TAVCV Nucleoprotein OS=Taro vein chlorosis virus OX=303300 GN=N PE=3 SV=1
MM1 pKa = 7.64 INTSRR6 pKa = 11.84 RR7 pKa = 11.84 SSRR10 pKa = 11.84 NKK12 pKa = 9.24 PATPAPEE19 pKa = 4.68 LIKK22 pKa = 10.91 AEE24 pKa = 4.2 EE25 pKa = 4.21 NPSDD29 pKa = 3.3 IHH31 pKa = 7.52 EE32 pKa = 5.15 DD33 pKa = 3.54 FADD36 pKa = 4.01 LPEE39 pKa = 4.8 PLLLSEE45 pKa = 4.9 ARR47 pKa = 11.84 AEE49 pKa = 4.0 NMPAVGSAISDD60 pKa = 3.79 LLKK63 pKa = 10.69 VEE65 pKa = 4.62 RR66 pKa = 11.84 AAPLLIGNTVEE77 pKa = 4.01 EE78 pKa = 4.78 MVTGRR83 pKa = 11.84 LADD86 pKa = 3.95 RR87 pKa = 11.84 GLGEE91 pKa = 3.77 LTEE94 pKa = 4.11 RR95 pKa = 11.84 EE96 pKa = 4.31 KK97 pKa = 10.76 IILAIGIHH105 pKa = 6.29 CGEE108 pKa = 4.46 SSMEE112 pKa = 3.89 HH113 pKa = 5.66 MNFLVTKK120 pKa = 9.84 RR121 pKa = 11.84 WITEE125 pKa = 3.81 EE126 pKa = 5.13 LKK128 pKa = 9.25 TQLTSLAATTRR139 pKa = 11.84 ALTEE143 pKa = 4.23 AGSLHH148 pKa = 5.82 RR149 pKa = 11.84 TYY151 pKa = 11.64 ALLQSPDD158 pKa = 3.27 QAKK161 pKa = 10.01 KK162 pKa = 10.58 QEE164 pKa = 4.36 ALDD167 pKa = 4.02 AMVTPQVGIDD177 pKa = 3.13 ITTLNHH183 pKa = 6.71 EE184 pKa = 4.35 GLEE187 pKa = 4.93 DD188 pKa = 2.9 IWQSYY193 pKa = 7.61 SHH195 pKa = 7.17 DD196 pKa = 3.82 AKK198 pKa = 11.13 VDD200 pKa = 3.44 AVDD203 pKa = 4.6 DD204 pKa = 3.88 YY205 pKa = 11.77 LRR207 pKa = 11.84 NILNVDD213 pKa = 3.72 PTPLYY218 pKa = 11.03 AEE220 pKa = 5.0 DD221 pKa = 2.99 EE222 pKa = 4.47 WGRR225 pKa = 11.84 HH226 pKa = 4.39 IAFIPRR232 pKa = 11.84 WQLVAYY238 pKa = 8.3 GKK240 pKa = 10.39 NSDD243 pKa = 3.61 QFKK246 pKa = 9.56 TVYY249 pKa = 10.52 ADD251 pKa = 3.68 EE252 pKa = 4.68 INYY255 pKa = 8.96 QRR257 pKa = 11.84 EE258 pKa = 4.0 SLEE261 pKa = 4.26 RR262 pKa = 11.84 ILAKK266 pKa = 10.01 RR267 pKa = 11.84 VKK269 pKa = 10.65 LNN271 pKa = 3.24
Molecular weight: 30.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.027
IPC2_protein 5.016
IPC_protein 4.952
Toseland 4.876
ProMoST 5.067
Dawson 4.914
Bjellqvist 5.054
Wikipedia 4.774
Rodwell 4.838
Grimsley 4.8
Solomon 4.914
Lehninger 4.876
Nozaki 5.041
DTASelect 5.169
Thurlkill 4.863
EMBOSS 4.813
Sillero 5.118
Patrickios 4.24
IPC_peptide 4.927
IPC2_peptide 5.105
IPC2.peptide.svr19 5.103
Protein with the highest isoelectric point:
>sp|Q5GA90|NCAP_TAVCV Nucleoprotein OS=Taro vein chlorosis virus OX=303300 GN=N PE=3 SV=1
MM1 pKa = 7.76 SYY3 pKa = 10.99 INIPDD8 pKa = 3.88 DD9 pKa = 3.9 VVSKK13 pKa = 11.28 YY14 pKa = 11.03 SDD16 pKa = 3.56 DD17 pKa = 4.76 LKK19 pKa = 10.48 TFTQKK24 pKa = 10.81 AGEE27 pKa = 4.19 IPSSKK32 pKa = 10.85 SLIPQTAYY40 pKa = 9.85 TIAALKK46 pKa = 9.59 TKK48 pKa = 10.58 LKK50 pKa = 9.96 FWEE53 pKa = 4.52 VTAKK57 pKa = 10.45 DD58 pKa = 4.23 DD59 pKa = 3.84 PTIASDD65 pKa = 3.21 WAGVCTAITAGTFSATNLKK84 pKa = 8.11 TVCEE88 pKa = 3.89 LAFNLRR94 pKa = 11.84 KK95 pKa = 9.12 PHH97 pKa = 5.1 EE98 pKa = 4.45 TGNVFIHH105 pKa = 6.13 TVPSDD110 pKa = 3.24 WTSSISTDD118 pKa = 3.75 SVDD121 pKa = 3.62 TTPIPATEE129 pKa = 4.03 SDD131 pKa = 3.46 ATLSTVSAAVQSGAAEE147 pKa = 4.15 DD148 pKa = 3.87 AATKK152 pKa = 10.57 AKK154 pKa = 10.4 AISFLCCALIRR165 pKa = 11.84 LSVKK169 pKa = 10.05 EE170 pKa = 4.33 PSHH173 pKa = 7.8 IMTAITSIRR182 pKa = 11.84 QRR184 pKa = 11.84 FGSLYY189 pKa = 9.97 GLASATLNAITFTRR203 pKa = 11.84 QQLSRR208 pKa = 11.84 IKK210 pKa = 10.53 QGIEE214 pKa = 3.87 TYY216 pKa = 10.58 SLARR220 pKa = 11.84 GTIFYY225 pKa = 9.36 YY226 pKa = 10.61 VRR228 pKa = 11.84 YY229 pKa = 10.1 ADD231 pKa = 3.66 TTYY234 pKa = 11.11 GSSDD238 pKa = 2.83 KK239 pKa = 11.18 SYY241 pKa = 10.43 GVCRR245 pKa = 11.84 FLLFQHH251 pKa = 6.78 LEE253 pKa = 4.08 LEE255 pKa = 4.52 GMHH258 pKa = 6.72 IYY260 pKa = 11.13 KK261 pKa = 9.84 MILALLTEE269 pKa = 4.88 WSTVPIGLLLTWIRR283 pKa = 11.84 NPKK286 pKa = 9.13 SALAVVEE293 pKa = 4.21 IKK295 pKa = 10.93 NIITNFDD302 pKa = 3.15 KK303 pKa = 11.43 AGVDD307 pKa = 3.64 KK308 pKa = 10.51 SWKK311 pKa = 7.56 YY312 pKa = 11.61 SRR314 pKa = 11.84 MIDD317 pKa = 3.03 NTFFLNISSRR327 pKa = 11.84 RR328 pKa = 11.84 NVYY331 pKa = 8.77 MCALLASLNKK341 pKa = 9.57 RR342 pKa = 11.84 HH343 pKa = 5.69 VPQGVGDD350 pKa = 3.81 YY351 pKa = 11.11 ADD353 pKa = 3.9 PRR355 pKa = 11.84 NIAVIKK361 pKa = 11.03 AMDD364 pKa = 4.01 AAVKK368 pKa = 9.95 NQVAIDD374 pKa = 3.61 VTLVEE379 pKa = 5.09 RR380 pKa = 11.84 IYY382 pKa = 11.04 EE383 pKa = 4.18 KK384 pKa = 11.28 YY385 pKa = 10.49 LISAGSTDD393 pKa = 3.7 AGTAYY398 pKa = 9.7 TLSRR402 pKa = 11.84 GTKK405 pKa = 8.84 RR406 pKa = 11.84 PNPAVFMSHH415 pKa = 5.34 QQAEE419 pKa = 4.19 GHH421 pKa = 5.16 PTKK424 pKa = 10.69 KK425 pKa = 9.45 RR426 pKa = 11.84 TWKK429 pKa = 10.86 SMVPPPPTLQLEE441 pKa = 4.43 DD442 pKa = 3.43 QQQRR446 pKa = 11.84 RR447 pKa = 11.84 LSPEE451 pKa = 3.47 LCEE454 pKa = 4.95 DD455 pKa = 3.9 CFSEE459 pKa = 4.43 LLRR462 pKa = 11.84 GKK464 pKa = 8.42 HH465 pKa = 5.69 HH466 pKa = 7.54 RR467 pKa = 11.84 ILPPPVIPSSSSEE480 pKa = 3.87 VLFSKK485 pKa = 10.47 SRR487 pKa = 11.84 IMEE490 pKa = 3.81 ISGGCSFDD498 pKa = 4.28 PIGFF502 pKa = 3.93
Molecular weight: 55.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.993
IPC2_protein 8.126
IPC_protein 7.98
Toseland 8.053
ProMoST 8.521
Dawson 8.799
Bjellqvist 8.931
Wikipedia 8.799
Rodwell 8.873
Grimsley 8.024
Solomon 8.887
Lehninger 8.873
Nozaki 9.092
DTASelect 8.741
Thurlkill 8.829
EMBOSS 8.96
Sillero 9.077
Patrickios 4.444
IPC_peptide 8.873
IPC2_peptide 7.732
IPC2.peptide.svr19 7.854
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
3811
235
1928
635.2
71.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.589 ± 0.878
1.811 ± 0.183
5.668 ± 0.514
5.432 ± 0.492
2.939 ± 0.238
6.166 ± 0.452
2.86 ± 0.289
7.373 ± 0.392
5.379 ± 0.236
10.575 ± 0.788
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.372
3.7 ± 0.252
4.854 ± 0.328
3.044 ± 0.079
4.933 ± 0.526
8.948 ± 0.428
6.534 ± 0.796
6.429 ± 0.309
1.863 ± 0.295
3.332 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here