Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1)
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8651 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F0ICY2|A0A0F0ICY2_ASPPU DLH domain-containing protein OS=Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1) OX=1403190 GN=P875_00010068 PE=4 SV=1
MM1 pKa = 7.7 ADD3 pKa = 3.51 EE4 pKa = 5.26 AGSIYY9 pKa = 10.71 DD10 pKa = 3.85 EE11 pKa = 4.95 IEE13 pKa = 4.34 IEE15 pKa = 5.79 DD16 pKa = 3.79 MTFDD20 pKa = 6.24 PITQLYY26 pKa = 9.73 HH27 pKa = 5.63 YY28 pKa = 7.55 PCPCGDD34 pKa = 3.67 RR35 pKa = 11.84 FEE37 pKa = 5.7 IMIDD41 pKa = 3.49 DD42 pKa = 4.19 LRR44 pKa = 11.84 DD45 pKa = 3.58 GEE47 pKa = 4.73 EE48 pKa = 3.84 IAVCPSCSLKK58 pKa = 10.28 IRR60 pKa = 11.84 VIFDD64 pKa = 3.02 VDD66 pKa = 4.13 DD67 pKa = 3.69 LHH69 pKa = 9.25 KK70 pKa = 10.81 DD71 pKa = 3.63 DD72 pKa = 4.29 QQQGPSAVAVQAA84 pKa = 4.09
Molecular weight: 9.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.714
IPC2_protein 3.808
IPC_protein 3.77
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.897
Patrickios 0.896
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A0F0IC83|A0A0F0IC83_ASPPU MATAHMG-box OS=Aspergillus parasiticus (strain ATCC 56775 / NRRL 5862 / SRRC 143 / SU-1) OX=1403190 GN=P875_00010325 PE=4 SV=1
MM1 pKa = 7.14 EE2 pKa = 4.95 QVSQPDD8 pKa = 3.49 QMNHH12 pKa = 5.02 FQHH15 pKa = 6.92 LNRR18 pKa = 11.84 IALHH22 pKa = 5.49 EE23 pKa = 4.22 FVRR26 pKa = 11.84 LPVSRR31 pKa = 11.84 EE32 pKa = 4.03 MVAHH36 pKa = 7.41 LAMQASQVIRR46 pKa = 11.84 CEE48 pKa = 3.89 PHH50 pKa = 4.85 VTTASAHH57 pKa = 5.34 GQPTPPSTPPLDD69 pKa = 4.68 AVDD72 pKa = 4.09 SQLPPLPSVEE82 pKa = 4.1 MFITSLVTRR91 pKa = 11.84 SQVQVPTLMTSLVYY105 pKa = 10.42 LARR108 pKa = 11.84 LRR110 pKa = 11.84 ARR112 pKa = 11.84 LPPVAKK118 pKa = 10.25 GMRR121 pKa = 11.84 CTVHH125 pKa = 7.16 RR126 pKa = 11.84 IFLASLILAAKK137 pKa = 9.3 NLNDD141 pKa = 3.64 SSPKK145 pKa = 7.95 NKK147 pKa = 9.25 HH148 pKa = 3.66 WARR151 pKa = 11.84 YY152 pKa = 4.24 TTVKK156 pKa = 10.64 GYY158 pKa = 10.95 DD159 pKa = 3.2 GFAFSLPEE167 pKa = 3.85 VNLMEE172 pKa = 4.37 RR173 pKa = 11.84 QLLFLLDD180 pKa = 3.01 WDD182 pKa = 4.16 TRR184 pKa = 11.84 VTEE187 pKa = 4.12 EE188 pKa = 5.79 DD189 pKa = 4.18 LLCHH193 pKa = 7.13 LEE195 pKa = 4.36 PFLAPIRR202 pKa = 11.84 YY203 pKa = 8.88 RR204 pKa = 11.84 YY205 pKa = 8.93 QVQEE209 pKa = 4.06 RR210 pKa = 11.84 EE211 pKa = 4.18 AEE213 pKa = 3.97 LRR215 pKa = 11.84 QPRR218 pKa = 11.84 EE219 pKa = 3.57 WRR221 pKa = 11.84 RR222 pKa = 11.84 LQASAEE228 pKa = 3.98 LLACRR233 pKa = 11.84 LRR235 pKa = 11.84 RR236 pKa = 11.84 QKK238 pKa = 11.1 LEE240 pKa = 3.57 ARR242 pKa = 11.84 LEE244 pKa = 3.93 ARR246 pKa = 11.84 RR247 pKa = 11.84 SDD249 pKa = 3.88 SIHH252 pKa = 6.1 RR253 pKa = 11.84 RR254 pKa = 11.84 QRR256 pKa = 11.84 LPASPASSVSLSSMSSNASPASLADD281 pKa = 3.57 TDD283 pKa = 3.38 RR284 pKa = 11.84 HH285 pKa = 5.3 KK286 pKa = 10.59 PYY288 pKa = 10.35 RR289 pKa = 11.84 PRR291 pKa = 11.84 RR292 pKa = 11.84 RR293 pKa = 11.84 PSTRR297 pKa = 11.84 SGASVSPPSAQDD309 pKa = 3.39 VPSLTRR315 pKa = 11.84 VDD317 pKa = 4.03 TVPSLSSRR325 pKa = 11.84 ASSIAPSSRR334 pKa = 11.84 NATPASLRR342 pKa = 11.84 TSSSITSMEE351 pKa = 3.45 ADD353 pKa = 2.84 IRR355 pKa = 11.84 VVDD358 pKa = 4.64 GARR361 pKa = 11.84 SPSLSCGYY369 pKa = 10.53 VPVSSMMAPNPKK381 pKa = 8.92 MEE383 pKa = 5.64 EE384 pKa = 3.74 IQQPTKK390 pKa = 10.35 KK391 pKa = 10.35 VRR393 pKa = 11.84 TNGHH397 pKa = 6.25 AGHH400 pKa = 6.84 TGFVARR406 pKa = 11.84 FLASAAGSYY415 pKa = 9.41 MGGRR419 pKa = 11.84 MRR421 pKa = 11.84 SHH423 pKa = 5.9 VV424 pKa = 3.46
Molecular weight: 47.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.516
IPC_protein 10.496
Toseland 10.526
ProMoST 10.321
Dawson 10.657
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.701
Grimsley 10.716
Solomon 10.789
Lehninger 10.745
Nozaki 10.54
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.95
Sillero 10.584
Patrickios 10.35
IPC_peptide 10.789
IPC2_peptide 9.604
IPC2.peptide.svr19 8.613
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8651
0
8651
4430027
66
6885
512.1
56.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.328 ± 0.021
1.263 ± 0.009
5.62 ± 0.017
6.118 ± 0.02
3.832 ± 0.014
6.881 ± 0.022
2.413 ± 0.011
5.15 ± 0.017
4.636 ± 0.021
9.163 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.009
3.748 ± 0.012
5.833 ± 0.027
3.999 ± 0.016
5.893 ± 0.02
8.199 ± 0.029
5.959 ± 0.016
6.307 ± 0.018
1.522 ± 0.009
2.965 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here