Corynebacterium testudinoris
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2543 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0G3HF05|A0A0G3HF05_9CORY DNA-binding protein excisionase family OS=Corynebacterium testudinoris OX=136857 GN=CTEST_11500 PE=4 SV=1
MM1 pKa = 7.58 ARR3 pKa = 11.84 TGSPIWIDD11 pKa = 4.16 LGTHH15 pKa = 7.16 DD16 pKa = 4.78 IDD18 pKa = 4.77 GSVSFYY24 pKa = 10.96 SQVFGWDD31 pKa = 3.47 VPEE34 pKa = 4.6 GAAEE38 pKa = 3.94 FGGYY42 pKa = 10.15 RR43 pKa = 11.84 IATKK47 pKa = 10.64 NGVPVGGMMSSLMGPDD63 pKa = 3.83 GPLEE67 pKa = 4.13 EE68 pKa = 5.07 PEE70 pKa = 4.98 YY71 pKa = 8.26 PTAWTVYY78 pKa = 10.35 LAVEE82 pKa = 4.32 DD83 pKa = 4.19 CAAAVAAAEE92 pKa = 4.33 KK93 pKa = 10.62 AGATVVVPAMEE104 pKa = 4.2 VGEE107 pKa = 4.55 LGSMAILFDD116 pKa = 3.85 PAGAGVGFWEE126 pKa = 4.43 AKK128 pKa = 9.43 EE129 pKa = 4.07 FPGLAFNGQPGTPVWFEE146 pKa = 3.75 QMSKK150 pKa = 10.86 DD151 pKa = 3.6 FQAAADD157 pKa = 5.01 FYY159 pKa = 11.54 QEE161 pKa = 3.85 ALGWNLAYY169 pKa = 9.98 MGEE172 pKa = 4.5 DD173 pKa = 3.62 GQPVDD178 pKa = 4.52 SPPTSGIRR186 pKa = 11.84 YY187 pKa = 5.9 ATNGAGEE194 pKa = 4.16 EE195 pKa = 4.16 ASAGLCEE202 pKa = 4.87 ADD204 pKa = 3.18 SFLPAEE210 pKa = 4.47 VPSYY214 pKa = 7.79 WRR216 pKa = 11.84 AYY218 pKa = 9.72 IGVSDD223 pKa = 4.35 TDD225 pKa = 3.14 ATAAQIVEE233 pKa = 4.3 LGGAVLDD240 pKa = 4.8 GPMDD244 pKa = 3.86 SPFGRR249 pKa = 11.84 VATVADD255 pKa = 3.97 PQGGTFQIVSVEE267 pKa = 4.05 GG268 pKa = 3.38
Molecular weight: 27.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.592
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 3.973
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.821
Patrickios 1.024
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A0G3H5Q1|A0A0G3H5Q1_9CORY Sphingosine/diacylglycerol kinase-like enzyme OS=Corynebacterium testudinoris OX=136857 GN=CTEST_06335 PE=4 SV=1
MM1 pKa = 7.41 TISPARR7 pKa = 11.84 LIALTLTWATIWSGAFVAMKK27 pKa = 10.16 VASQSAGAFAIVGIRR42 pKa = 11.84 CTIAGAIMIAAAWGARR58 pKa = 11.84 KK59 pKa = 9.64 ALTRR63 pKa = 11.84 SGVKK67 pKa = 8.61 GTIVIGILNNLGYY80 pKa = 10.48 LGLMASAMPYY90 pKa = 10.1 VSAGMAAIITALTPLAVLVISALRR114 pKa = 11.84 GRR116 pKa = 11.84 ALLITQWIGCVVGFLGVVGSAWTRR140 pKa = 11.84 LDD142 pKa = 3.34 SGEE145 pKa = 4.24 TQWRR149 pKa = 11.84 GILFALGAVACLVAGTVLTPKK170 pKa = 10.06 LVPPGSPWLSTGIQSLAAGIPALGLSLLFEE200 pKa = 4.57 DD201 pKa = 5.01 TPTFSTPFIVAEE213 pKa = 4.46 AYY215 pKa = 10.53 LILGASVLGMTMWLQLIRR233 pKa = 11.84 EE234 pKa = 4.74 AGPDD238 pKa = 3.25 RR239 pKa = 11.84 AAVAHH244 pKa = 6.6 FLPPLISIALGALILGEE261 pKa = 4.24 AVSPLALAMCIPVAIGVALATRR283 pKa = 11.84 RR284 pKa = 11.84 PRR286 pKa = 11.84 KK287 pKa = 9.21 PRR289 pKa = 11.84 QLL291 pKa = 3.42
Molecular weight: 29.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.57
ProMoST 10.277
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.847
Grimsley 10.73
Solomon 10.789
Lehninger 10.76
Nozaki 10.599
DTASelect 10.394
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.613
Patrickios 10.599
IPC_peptide 10.789
IPC2_peptide 9.706
IPC2.peptide.svr19 8.531
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2543
0
2543
818509
39
3015
321.9
34.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.736 ± 0.056
0.629 ± 0.013
6.15 ± 0.042
6.004 ± 0.053
3.304 ± 0.03
8.489 ± 0.044
2.157 ± 0.024
5.175 ± 0.035
2.576 ± 0.033
10.108 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.227 ± 0.018
2.598 ± 0.027
5.193 ± 0.03
3.114 ± 0.03
6.444 ± 0.041
5.886 ± 0.031
6.192 ± 0.034
8.417 ± 0.05
1.462 ± 0.021
2.138 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here