Amino acid dipepetide frequency for Ensifer adhaerens OV14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.677AlaAla: 15.677 ± 0.117	0.964AlaCys: 0.964 ± 0.025	6.702AlaAsp: 6.702 ± 0.069	7.388AlaGlu: 7.388 ± 0.07	4.756AlaPhe: 4.756 ± 0.055	10.242AlaGly: 10.242 ± 0.086	2.067AlaHis: 2.067 ± 0.037	7.127AlaIle: 7.127 ± 0.071	4.304AlaLys: 4.304 ± 0.065	12.775AlaLeu: 12.775 ± 0.097	3.507AlaMet: 3.507 ± 0.049	3.079AlaAsn: 3.079 ± 0.045	4.631AlaPro: 4.631 ± 0.056	3.342AlaGln: 3.342 ± 0.045	7.794AlaArg: 7.794 ± 0.077	6.511AlaSer: 6.511 ± 0.058	5.946AlaThr: 5.946 ± 0.062	8.648AlaVal: 8.648 ± 0.076	1.342AlaTrp: 1.342 ± 0.027	2.615AlaTyr: 2.615 ± 0.046	0.002AlaXaa: 0.002 ± 0.001
Cys	0.867CysAla: 0.867 ± 0.023	0.109CysCys: 0.109 ± 0.009	0.502CysAsp: 0.502 ± 0.018	0.427CysGlu: 0.427 ± 0.016	0.314CysPhe: 0.314 ± 0.014	0.862CysGly: 0.862 ± 0.024	0.228CysHis: 0.228 ± 0.01	0.417CysIle: 0.417 ± 0.016	0.184CysLys: 0.184 ± 0.009	0.796CysLeu: 0.796 ± 0.022	0.156CysMet: 0.156 ± 0.01	0.194CysAsn: 0.194 ± 0.009	0.367CysPro: 0.367 ± 0.015	0.227CysGln: 0.227 ± 0.012	0.571CysArg: 0.571 ± 0.019	0.423CysSer: 0.423 ± 0.015	0.388CysThr: 0.388 ± 0.014	0.591CysVal: 0.591 ± 0.019	0.11CysTrp: 0.11 ± 0.008	0.202CysTyr: 0.202 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.51AspAla: 6.51 ± 0.064	0.496AspCys: 0.496 ± 0.015	3.042AspAsp: 3.042 ± 0.053	3.552AspGlu: 3.552 ± 0.049	2.319AspPhe: 2.319 ± 0.043	5.22AspGly: 5.22 ± 0.065	1.218AspHis: 1.218 ± 0.026	3.48AspIle: 3.48 ± 0.048	1.848AspLys: 1.848 ± 0.037	5.694AspLeu: 5.694 ± 0.054	1.411AspMet: 1.411 ± 0.028	1.464AspAsn: 1.464 ± 0.031	3.223AspPro: 3.223 ± 0.049	1.701AspGln: 1.701 ± 0.033	4.224AspArg: 4.224 ± 0.052	1.988AspSer: 1.988 ± 0.034	2.504AspThr: 2.504 ± 0.044	4.392AspVal: 4.392 ± 0.051	0.858AspTrp: 0.858 ± 0.023	1.51AspTyr: 1.51 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	7.352GluAla: 7.352 ± 0.066	0.329GluCys: 0.329 ± 0.014	2.72GluAsp: 2.72 ± 0.042	3.299GluGlu: 3.299 ± 0.055	1.882GluPhe: 1.882 ± 0.035	4.267GluGly: 4.267 ± 0.049	1.228GluHis: 1.228 ± 0.024	3.879GluIle: 3.879 ± 0.052	2.663GluLys: 2.663 ± 0.041	5.399GluLeu: 5.399 ± 0.062	1.615GluMet: 1.615 ± 0.034	1.841GluAsn: 1.841 ± 0.027	2.738GluPro: 2.738 ± 0.045	2.107GluGln: 2.107 ± 0.045	4.862GluArg: 4.862 ± 0.054	2.25GluSer: 2.25 ± 0.038	3.711GluThr: 3.711 ± 0.05	3.743GluVal: 3.743 ± 0.049	0.683GluTrp: 0.683 ± 0.017	1.033GluTyr: 1.033 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.7PheAla: 4.7 ± 0.053	0.385PheCys: 0.385 ± 0.016	2.76PheAsp: 2.76 ± 0.042	2.217PheGlu: 2.217 ± 0.035	1.602PhePhe: 1.602 ± 0.039	3.845PheGly: 3.845 ± 0.056	0.775PheHis: 0.775 ± 0.02	1.967PheIle: 1.967 ± 0.035	1.117PheLys: 1.117 ± 0.028	3.693PheLeu: 3.693 ± 0.048	0.916PheMet: 0.916 ± 0.023	1.214PheAsn: 1.214 ± 0.026	1.618PhePro: 1.618 ± 0.032	1.125PheGln: 1.125 ± 0.025	2.348PheArg: 2.348 ± 0.037	2.515PheSer: 2.515 ± 0.035	2.019PheThr: 2.019 ± 0.036	3.178PheVal: 3.178 ± 0.044	0.578PheTrp: 0.578 ± 0.022	1.008PheTyr: 1.008 ± 0.026	0.0PheXaa: 0.0 ± 0.0
Gly	8.678GlyAla: 8.678 ± 0.063	0.803GlyCys: 0.803 ± 0.02	4.239GlyAsp: 4.239 ± 0.055	4.923GlyGlu: 4.923 ± 0.062	3.69GlyPhe: 3.69 ± 0.046	6.956GlyGly: 6.956 ± 0.084	1.884GlyHis: 1.884 ± 0.037	5.062GlyIle: 5.062 ± 0.057	3.692GlyLys: 3.692 ± 0.053	8.889GlyLeu: 8.889 ± 0.079	2.308GlyMet: 2.308 ± 0.037	2.425GlyAsn: 2.425 ± 0.046	3.14GlyPro: 3.14 ± 0.042	2.762GlyGln: 2.762 ± 0.042	5.838GlyArg: 5.838 ± 0.062	4.65GlySer: 4.65 ± 0.053	4.488GlyThr: 4.488 ± 0.06	6.126GlyVal: 6.126 ± 0.072	1.227GlyTrp: 1.227 ± 0.029	2.4GlyTyr: 2.4 ± 0.044	0.001GlyXaa: 0.001 ± 0.001
His	2.213HisAla: 2.213 ± 0.035	0.213HisCys: 0.213 ± 0.011	1.216HisAsp: 1.216 ± 0.027	1.066HisGlu: 1.066 ± 0.023	0.931HisPhe: 0.931 ± 0.021	1.842HisGly: 1.842 ± 0.033	0.529HisHis: 0.529 ± 0.021	0.971HisIle: 0.971 ± 0.024	0.513HisLys: 0.513 ± 0.017	2.058HisLeu: 2.058 ± 0.037	0.55HisMet: 0.55 ± 0.017	0.475HisAsn: 0.475 ± 0.016	1.244HisPro: 1.244 ± 0.029	0.573HisGln: 0.573 ± 0.017	1.392HisArg: 1.392 ± 0.028	0.968HisSer: 0.968 ± 0.024	0.796HisThr: 0.796 ± 0.021	1.516HisVal: 1.516 ± 0.03	0.287HisTrp: 0.287 ± 0.013	0.577HisTyr: 0.577 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	8.071IleAla: 8.071 ± 0.067	0.562IleCys: 0.562 ± 0.019	3.928IleAsp: 3.928 ± 0.047	3.78IleGlu: 3.78 ± 0.042	2.057IlePhe: 2.057 ± 0.035	5.422IleGly: 5.422 ± 0.059	0.977IleHis: 0.977 ± 0.024	2.774IleIle: 2.774 ± 0.046	1.649IleLys: 1.649 ± 0.031	4.974IleLeu: 4.974 ± 0.059	1.155IleMet: 1.155 ± 0.025	1.634IleAsn: 1.634 ± 0.029	2.366IlePro: 2.366 ± 0.042	1.285IleGln: 1.285 ± 0.026	3.478IleArg: 3.478 ± 0.047	3.333IleSer: 3.333 ± 0.043	2.842IleThr: 2.842 ± 0.045	4.81IleVal: 4.81 ± 0.053	0.618IleTrp: 0.618 ± 0.021	1.27IleTyr: 1.27 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.649LysAla: 4.649 ± 0.061	0.15LysCys: 0.15 ± 0.009	1.996LysAsp: 1.996 ± 0.039	1.834LysGlu: 1.834 ± 0.037	0.982LysPhe: 0.982 ± 0.022	2.904LysGly: 2.904 ± 0.04	0.677LysHis: 0.677 ± 0.02	1.999LysIle: 1.999 ± 0.036	1.434LysLys: 1.434 ± 0.032	3.653LysLeu: 3.653 ± 0.048	0.88LysMet: 0.88 ± 0.022	1.009LysAsn: 1.009 ± 0.025	2.185LysPro: 2.185 ± 0.037	1.105LysGln: 1.105 ± 0.028	2.478LysArg: 2.478 ± 0.037	2.117LysSer: 2.117 ± 0.037	2.24LysThr: 2.24 ± 0.042	2.745LysVal: 2.745 ± 0.046	0.392LysTrp: 0.392 ± 0.016	0.674LysTyr: 0.674 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	12.844LeuAla: 12.844 ± 0.096	0.847LeuCys: 0.847 ± 0.02	5.894LeuAsp: 5.894 ± 0.06	5.355LeuGlu: 5.355 ± 0.049	3.779LeuPhe: 3.779 ± 0.048	8.166LeuGly: 8.166 ± 0.08	1.828LeuHis: 1.828 ± 0.035	5.349LeuIle: 5.349 ± 0.075	4.133LeuLys: 4.133 ± 0.047	9.348LeuLeu: 9.348 ± 0.103	2.463LeuMet: 2.463 ± 0.035	2.742LeuAsn: 2.742 ± 0.044	5.278LeuPro: 5.278 ± 0.059	2.81LeuGln: 2.81 ± 0.039	6.299LeuArg: 6.299 ± 0.07	7.072LeuSer: 7.072 ± 0.067	5.734LeuThr: 5.734 ± 0.057	7.654LeuVal: 7.654 ± 0.07	1.066LeuTrp: 1.066 ± 0.027	2.136LeuTyr: 2.136 ± 0.035	0.001LeuXaa: 0.001 ± 0.001
Met	3.112MetAla: 3.112 ± 0.042	0.154MetCys: 0.154 ± 0.009	1.157MetAsp: 1.157 ± 0.025	1.219MetGlu: 1.219 ± 0.025	0.781MetPhe: 0.781 ± 0.021	1.775MetGly: 1.775 ± 0.034	0.452MetHis: 0.452 ± 0.017	1.577MetIle: 1.577 ± 0.03	1.153MetLys: 1.153 ± 0.024	2.709MetLeu: 2.709 ± 0.04	0.784MetMet: 0.784 ± 0.022	0.863MetAsn: 0.863 ± 0.022	1.487MetPro: 1.487 ± 0.029	0.888MetGln: 0.888 ± 0.021	1.87MetArg: 1.87 ± 0.034	1.701MetSer: 1.701 ± 0.028	1.97MetThr: 1.97 ± 0.031	1.852MetVal: 1.852 ± 0.032	0.203MetTrp: 0.203 ± 0.011	0.341MetTyr: 0.341 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.362AsnAla: 3.362 ± 0.045	0.232AsnCys: 0.232 ± 0.011	1.581AsnAsp: 1.581 ± 0.039	1.372AsnGlu: 1.372 ± 0.027	1.071AsnPhe: 1.071 ± 0.026	2.658AsnGly: 2.658 ± 0.049	0.529AsnHis: 0.529 ± 0.016	1.561AsnIle: 1.561 ± 0.028	0.743AsnLys: 0.743 ± 0.017	2.79AsnLeu: 2.79 ± 0.036	0.716AsnMet: 0.716 ± 0.017	0.814AsnAsn: 0.814 ± 0.024	1.919AsnPro: 1.919 ± 0.03	0.798AsnGln: 0.798 ± 0.02	1.921AsnArg: 1.921 ± 0.036	1.319AsnSer: 1.319 ± 0.031	1.385AsnThr: 1.385 ± 0.034	2.083AsnVal: 2.083 ± 0.035	0.464AsnTrp: 0.464 ± 0.017	0.712AsnTyr: 0.712 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	5.37ProAla: 5.37 ± 0.066	0.273ProCys: 0.273 ± 0.012	3.316ProAsp: 3.316 ± 0.05	3.548ProGlu: 3.548 ± 0.041	2.017ProPhe: 2.017 ± 0.032	4.031ProGly: 4.031 ± 0.048	1.036ProHis: 1.036 ± 0.024	2.353ProIle: 2.353 ± 0.035	1.724ProLys: 1.724 ± 0.031	4.643ProLeu: 4.643 ± 0.056	1.186ProMet: 1.186 ± 0.028	1.331ProAsn: 1.331 ± 0.027	1.963ProPro: 1.963 ± 0.038	1.551ProGln: 1.551 ± 0.029	2.464ProArg: 2.464 ± 0.045	2.773ProSer: 2.773 ± 0.041	2.484ProThr: 2.484 ± 0.036	4.093ProVal: 4.093 ± 0.058	0.625ProTrp: 0.625 ± 0.019	1.192ProTyr: 1.192 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	3.804GlnAla: 3.804 ± 0.05	0.164GlnCys: 0.164 ± 0.011	1.364GlnAsp: 1.364 ± 0.026	1.553GlnGlu: 1.553 ± 0.028	1.103GlnPhe: 1.103 ± 0.026	2.176GlnGly: 2.176 ± 0.035	0.593GlnHis: 0.593 ± 0.019	1.928GlnIle: 1.928 ± 0.035	1.202GlnLys: 1.202 ± 0.025	2.852GlnLeu: 2.852 ± 0.043	0.96GlnMet: 0.96 ± 0.025	0.826GlnAsn: 0.826 ± 0.024	1.629GlnPro: 1.629 ± 0.032	1.27GlnGln: 1.27 ± 0.031	2.295GlnArg: 2.295 ± 0.046	1.869GlnSer: 1.869 ± 0.035	1.733GlnThr: 1.733 ± 0.033	2.1GlnVal: 2.1 ± 0.032	0.402GlnTrp: 0.402 ± 0.015	0.617GlnTyr: 0.617 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	7.056ArgAla: 7.056 ± 0.067	0.466ArgCys: 0.466 ± 0.019	3.867ArgAsp: 3.867 ± 0.053	3.924ArgGlu: 3.924 ± 0.049	2.894ArgPhe: 2.894 ± 0.039	4.395ArgGly: 4.395 ± 0.057	1.667ArgHis: 1.667 ± 0.036	4.059ArgIle: 4.059 ± 0.05	2.538ArgLys: 2.538 ± 0.044	7.492ArgLeu: 7.492 ± 0.087	1.9ArgMet: 1.9 ± 0.033	2.01ArgAsn: 2.01 ± 0.034	3.15ArgPro: 3.15 ± 0.045	2.645ArgGln: 2.645 ± 0.049	5.077ArgArg: 5.077 ± 0.074	3.768ArgSer: 3.768 ± 0.049	3.295ArgThr: 3.295 ± 0.041	4.479ArgVal: 4.479 ± 0.053	0.825ArgTrp: 0.825 ± 0.021	1.641ArgTyr: 1.641 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.179SerAla: 6.179 ± 0.055	0.398SerCys: 0.398 ± 0.017	3.051SerAsp: 3.051 ± 0.041	3.072SerGlu: 3.072 ± 0.039	2.534SerPhe: 2.534 ± 0.04	5.81SerGly: 5.81 ± 0.061	1.12SerHis: 1.12 ± 0.023	3.187SerIle: 3.187 ± 0.048	1.868SerLys: 1.868 ± 0.036	5.709SerLeu: 5.709 ± 0.058	1.439SerMet: 1.439 ± 0.028	1.495SerAsn: 1.495 ± 0.03	2.646SerPro: 2.646 ± 0.038	1.641SerGln: 1.641 ± 0.029	3.643SerArg: 3.643 ± 0.054	3.077SerSer: 3.077 ± 0.048	2.889SerThr: 2.889 ± 0.039	4.27SerVal: 4.27 ± 0.045	0.766SerTrp: 0.766 ± 0.024	1.382SerTyr: 1.382 ± 0.031	0.001SerXaa: 0.001 ± 0.001
Thr	6.183ThrAla: 6.183 ± 0.056	0.405ThrCys: 0.405 ± 0.015	2.876ThrAsp: 2.876 ± 0.042	2.763ThrGlu: 2.763 ± 0.041	2.236ThrPhe: 2.236 ± 0.035	4.988ThrGly: 4.988 ± 0.062	1.011ThrHis: 1.011 ± 0.025	3.245ThrIle: 3.245 ± 0.043	1.634ThrLys: 1.634 ± 0.033	5.839ThrLeu: 5.839 ± 0.069	1.33ThrMet: 1.33 ± 0.029	1.369ThrAsn: 1.369 ± 0.03	2.975ThrPro: 2.975 ± 0.044	1.389ThrGln: 1.389 ± 0.025	3.157ThrArg: 3.157 ± 0.047	2.947ThrSer: 2.947 ± 0.042	3.041ThrThr: 3.041 ± 0.047	4.555ThrVal: 4.555 ± 0.051	0.635ThrTrp: 0.635 ± 0.019	1.265ThrTyr: 1.265 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	9.133ValAla: 9.133 ± 0.095	0.618ValCys: 0.618 ± 0.018	4.318ValAsp: 4.318 ± 0.048	4.549ValGlu: 4.549 ± 0.051	3.075ValPhe: 3.075 ± 0.047	5.669ValGly: 5.669 ± 0.068	1.361ValHis: 1.361 ± 0.028	4.362ValIle: 4.362 ± 0.051	2.499ValLys: 2.499 ± 0.044	7.539ValLeu: 7.539 ± 0.083	1.957ValMet: 1.957 ± 0.034	2.159ValAsn: 2.159 ± 0.037	3.573ValPro: 3.573 ± 0.039	1.924ValGln: 1.924 ± 0.03	4.789ValArg: 4.789 ± 0.055	4.798ValSer: 4.798 ± 0.05	4.568ValThr: 4.568 ± 0.051	6.108ValVal: 6.108 ± 0.069	0.836ValTrp: 0.836 ± 0.022	1.567ValTyr: 1.567 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.111TrpAla: 1.111 ± 0.027	0.117TrpCys: 0.117 ± 0.007	0.603TrpAsp: 0.603 ± 0.019	0.505TrpGlu: 0.505 ± 0.018	0.549TrpPhe: 0.549 ± 0.019	0.803TrpGly: 0.803 ± 0.025	0.281TrpHis: 0.281 ± 0.013	0.667TrpIle: 0.667 ± 0.02	0.512TrpLys: 0.512 ± 0.019	1.563TrpLeu: 1.563 ± 0.035	0.331TrpMet: 0.331 ± 0.013	0.468TrpAsn: 0.468 ± 0.017	0.647TrpPro: 0.647 ± 0.018	0.567TrpGln: 0.567 ± 0.019	0.942TrpArg: 0.942 ± 0.023	0.806TrpSer: 0.806 ± 0.024	0.731TrpThr: 0.731 ± 0.021	0.771TrpVal: 0.771 ± 0.022	0.235TrpTrp: 0.235 ± 0.012	0.296TrpTyr: 0.296 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.544TyrAla: 2.544 ± 0.041	0.248TyrCys: 0.248 ± 0.013	1.522TyrAsp: 1.522 ± 0.037	1.256TyrGlu: 1.256 ± 0.026	1.007TyrPhe: 1.007 ± 0.025	2.152TyrGly: 2.152 ± 0.035	0.471TyrHis: 0.471 ± 0.017	1.023TyrIle: 1.023 ± 0.026	0.691TyrLys: 0.691 ± 0.023	2.379TyrLeu: 2.379 ± 0.039	0.484TyrMet: 0.484 ± 0.016	0.62TyrAsn: 0.62 ± 0.018	1.194TyrPro: 1.194 ± 0.023	0.731TyrGln: 0.731 ± 0.02	1.709TyrArg: 1.709 ± 0.028	1.289TyrSer: 1.289 ± 0.028	1.079TyrThr: 1.079 ± 0.027	1.688TyrVal: 1.688 ± 0.028	0.352TyrTrp: 0.352 ± 0.017	0.61TyrTyr: 0.61 ± 0.017	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.2XaaXaa: 0.2 ± 0.091

Statistics based on 5422 proteins (1788006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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