Atta colombica
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14143 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A195BD99|A0A195BD99_9HYME Tau-tubulin kinase 1 OS=Atta colombica OX=520822 GN=ALC53_07422 PE=4 SV=1
MM1 pKa = 7.16 FCKK4 pKa = 10.33 IKK6 pKa = 9.89 TKK8 pKa = 10.15 RR9 pKa = 11.84 PRR11 pKa = 11.84 SAEE14 pKa = 4.02 VAFEE18 pKa = 3.87 EE19 pKa = 4.71 ANRR22 pKa = 11.84 SIDD25 pKa = 3.64 PVDD28 pKa = 3.77 GKK30 pKa = 10.79 NSGSVDD36 pKa = 3.54 DD37 pKa = 5.29 AFLKK41 pKa = 10.64 ARR43 pKa = 11.84 EE44 pKa = 4.13 TRR46 pKa = 11.84 GSIDD50 pKa = 3.08 CAYY53 pKa = 10.46 ALEE56 pKa = 4.39 RR57 pKa = 11.84 FMRR60 pKa = 11.84 EE61 pKa = 4.2 YY62 pKa = 11.02 YY63 pKa = 10.38 SVPGSLMIIEE73 pKa = 4.88 NYY75 pKa = 7.84 YY76 pKa = 10.7 HH77 pKa = 7.54 CDD79 pKa = 3.43 DD80 pKa = 6.11 DD81 pKa = 4.62 EE82 pKa = 7.27 DD83 pKa = 4.71 NDD85 pKa = 5.09 EE86 pKa = 5.2 EE87 pKa = 6.78 DD88 pKa = 5.99 DD89 pKa = 6.09 DD90 pKa = 7.44 DD91 pKa = 7.59 DD92 pKa = 7.59 DD93 pKa = 6.9 DD94 pKa = 6.78 FDD96 pKa = 6.76 DD97 pKa = 5.55 DD98 pKa = 4.46 YY99 pKa = 11.87 YY100 pKa = 11.7 YY101 pKa = 11.36 YY102 pKa = 10.7 YY103 pKa = 11.15 DD104 pKa = 4.2 NIINII109 pKa = 4.11
Molecular weight: 12.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.884
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.846
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.902
Protein with the highest isoelectric point:
>tr|A0A195BCH2|A0A195BCH2_9HYME Uncharacterized protein OS=Atta colombica OX=520822 GN=ALC53_07686 PE=4 SV=1
MM1 pKa = 7.2 VSCSSSQPWKK11 pKa = 10.72 VIEE14 pKa = 4.58 KK15 pKa = 10.3 LDD17 pKa = 3.53 IFISRR22 pKa = 11.84 LRR24 pKa = 11.84 GCEE27 pKa = 3.35 ASEE30 pKa = 4.07 PVHH33 pKa = 5.97 EE34 pKa = 4.6 RR35 pKa = 11.84 ASAFNLASSSNFRR48 pKa = 11.84 HH49 pKa = 5.57 NASVNIFIAYY59 pKa = 8.55 LARR62 pKa = 11.84 PEE64 pKa = 3.97 WAGSNNKK71 pKa = 9.11 LLCLTFSANSCAVGAILHH89 pKa = 6.15 ARR91 pKa = 11.84 ALLLAVLSCLLIIALRR107 pKa = 11.84 FLAGLPRR114 pKa = 11.84 LRR116 pKa = 11.84 SLLLLLLLFLLRR128 pKa = 11.84 VGWHH132 pKa = 6.33 CLSPGIYY139 pKa = 9.22 QRR141 pKa = 11.84 LRR143 pKa = 11.84 LRR145 pKa = 11.84 YY146 pKa = 7.75 PWRR149 pKa = 11.84 EE150 pKa = 3.38 EE151 pKa = 4.15 SQRR154 pKa = 11.84 SAHH157 pKa = 6.05 GVFARR162 pKa = 11.84 YY163 pKa = 8.92 VRR165 pKa = 11.84 HH166 pKa = 6.58 RR167 pKa = 11.84 LGTCVIRR174 pKa = 11.84 KK175 pKa = 9.19 GKK177 pKa = 9.82 ILWEE181 pKa = 4.22 RR182 pKa = 11.84 KK183 pKa = 9.04 RR184 pKa = 11.84 EE185 pKa = 4.09 LTYY188 pKa = 11.07 GVASLIKK195 pKa = 10.47 SRR197 pKa = 11.84 NDD199 pKa = 3.23 CDD201 pKa = 3.91 AKK203 pKa = 11.13
Molecular weight: 22.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.545
IPC_protein 10.248
Toseland 10.452
ProMoST 10.218
Dawson 10.599
Bjellqvist 10.335
Wikipedia 10.804
Rodwell 10.789
Grimsley 10.657
Solomon 10.687
Lehninger 10.643
Nozaki 10.511
DTASelect 10.306
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.482
IPC_peptide 10.687
IPC2_peptide 9.692
IPC2.peptide.svr19 8.462
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14143
0
14143
6716947
18
20976
474.9
53.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.979 ± 0.02
2.055 ± 0.028
5.333 ± 0.02
6.619 ± 0.034
3.658 ± 0.019
5.223 ± 0.027
2.553 ± 0.012
6.272 ± 0.024
6.375 ± 0.031
9.008 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.333 ± 0.01
5.2 ± 0.021
4.85 ± 0.028
4.167 ± 0.02
5.947 ± 0.024
8.249 ± 0.027
6.029 ± 0.018
5.838 ± 0.014
1.073 ± 0.008
3.198 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here